scholarly journals Electric field mediated fibronectin-hydroxyapatite interaction: A molecular insight

2020 ◽  
Author(s):  
Subhadip Basu ◽  
Biswajit Gorai ◽  
Bikramjit Basu ◽  
Prabal K. Maiti
Keyword(s):  
Field Strength ◽  
Electric Field ◽  
Protein Adsorption ◽  
Structural Integrity ◽  
Critical Role ◽  
Field Stimulation ◽  
Electric Field Stimulation ◽  
Cell Material ◽  
Material Interaction

AbstractIn experimental research driven biomaterials science, the influence of different material properties (elastic stiffness, surface energy, etc.), and to a relatively lesser extent, the biophysical stimulation (electric/magnetic) on the cell-material interaction has been extensively investigated. Considering the central importance of the protein adsorption on cell-material interaction, the role of physiochemical factors on the protein adsorption is also probed. Despite its significance, the quantitative analysis of many such aspects remains largely unexplored in biomaterials science. In recent studies, the critical role of electric field stimulation towards modulation of cell functionality on implantable biomaterials has been experimentally demonstrated. Given this background, we investigated the influence of external electric field stimulation (upto 1.00 V/nm) on fibronectin (FN) adsorption on hydroxyapatite, HA (100) surface at 300K using all-atom MD simulation method. Fibronectin adsorption was found to be governed by the attractive electrostatic interaction, which changed with the electric field strength. Non-monotonous changes in structural integrity of fibronectin were recorded with the change in field strength and direction. This can be attributed to the spatial rearrangement of local charges and global structural changes of the protein. The dipole moment vectors of fibronectin, water and HA quantitatively exhibited similar pattern of orienting themselves parallel to the field direction, with field strength dependent increase in their magnitudes. No significant change has been recorded for radial distribution function of water surrounding fibronectin. Field dependent variation in the salt bridge nets and number of hydrogen bonds between fibronectin and hydroxyapatite were also examined. One of the important results in the context of the cell-material interaction is that the RGD sequence of FN was exposed to solvent side, when the field was applied along a direction outward perpendicular to HA (001) surface. Summarizing, the present study provides quantitative insights into the influence of electric field stimulation on biomolecular interactions involved in fibronectin adsorption on hydroxyapatite surface.

scholarly journals Role of cortical cell type and morphology in subthreshold and suprathreshold uniform electric field stimulation in vitro

2009 ◽  
Vol 2 (4) ◽  
pp. 215-228.e3 ◽  
Author(s):  
Thomas Radman ◽  
Raddy L. Ramos ◽  
Joshua C. Brumberg ◽  
Marom Bikson
Keyword(s):  
Electric Field ◽  
Cortical Cell ◽  
Uniform Electric Field ◽  
Field Stimulation ◽  
Cell Type ◽  
Electric Field Stimulation

Role of cortical cell type and neuronal morphology in electric field stimulation

2008 ◽  
Vol 1 (3) ◽  
pp. 247-248 ◽  
Author(s):  
T. Radman ◽  
R. Ramos ◽  
J.C. Brumberg ◽  
M. Bikson ◽  
J.C. Brumberg
Keyword(s):  
Electric Field ◽  
Cortical Cell ◽  
Neuronal Morphology ◽  
Field Stimulation ◽  
Cell Type ◽  
Electric Field Stimulation

scholarly journals Ih interacts with somato-dendritic structure to determine frequency response to weak alternating electric field stimulation

2018 ◽  
Vol 119 (3) ◽  
pp. 1029-1036 ◽  
Author(s):  
Enrique H. S. Toloza ◽  
Ehsan Negahbani ◽  
Flavio Fröhlich
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Cellular Response ◽  
Current Injection ◽  
Field Stimulation ◽  
Electric Field Stimulation ◽  
Cation Current ◽  
Network Oscillations ◽  
Alternating Electric Field

Transcranial current stimulation (tCS) modulates brain dynamics using weak electric fields. Given the pathological changes in brain network oscillations in neurological and psychiatric illnesses, using alternating electric field waveforms that engage rhythmic activity has been proposed as a targeted, network-level treatment approach. Previous studies have investigated the effects of electric fields at the neuronal level. However, the biophysical basis of the cellular response to electric fields has remained limited. Here, we characterized the frequency-dependent response of different compartments in a layer V pyramidal neuron to exogenous electric fields to dissect the relative contributions of voltage-gated ion channels and neuronal morphology. Hyperpolarization-activated cation current (Ih) in the distal dendrites was the primary ionic mechanism shaping the model’s response to electric field stimulation and caused subthreshold resonance in the tuft at 20 ± 4 Hz. In contrast, subthreshold Ih-mediated resonance in response to local sinusoidal current injection was present in all model compartments at 11 ± 2 Hz. The frequencies of both resonance responses were modulated by Ih conductance density. We found that the difference in resonance frequency between the two stimulation types can be explained by the fact that exogenous electric fields simultaneously polarize the membrane potentials at the distal ends of the neuron (relative to field direction) in opposite directions. Our results highlight the role of Ih in shaping the cellular response to electric field stimulation and suggest that the common model of tCS as a weak somatic current injection fails to capture the cellular effects of electric field stimulation. NEW & NOTEWORTHY Modulation of cortical oscillation by brain stimulation serves as a tool to understand the causal role of network oscillations in behavior and is a potential treatment modality that engages impaired network oscillations in disorders of the central nervous system. To develop targeted stimulation paradigms, cellular-level effects must be understood. We demonstrate that hyperpolarization-activated cation current (Ih) and cell morphology cooperatively shape the response to applied alternating electric fields.

Electric Field Stimulation of Delayed Fluorescence in Dry Films of Chloroplasts and Subchloroplast Particles Enriched in PSII

1979 ◽  
Vol 174 (2) ◽  
pp. 85-91 ◽  
Author(s):  
T.V. Ortoidze ◽  
Galina P. Borisevitch ◽  
P.S. Venediktov ◽  
A.A. Kononenko ◽  
D.N. Matorin ◽  
...  
Keyword(s):  
Electric Field ◽  
Delayed Fluorescence ◽  
Field Stimulation ◽  
Electric Field Stimulation ◽  
Stimulation Of

Neuromodulation‐dependent effect of gated high‐frequency, LFMS ‐like electric field stimulation in mouse cortical slices

2018 ◽  
Author(s):  
Ehsan Negahbani ◽  
Stephen L. Schmidt ◽  
Nadia Mishal ◽  
Flavio Fröhlich
Keyword(s):  
Electric Field ◽  
High Frequency ◽  
Field Stimulation ◽  
Electric Field Stimulation ◽  
Dependent Effect ◽  
Cortical Slices

scholarly journals Electric field stimulation through a biodegradable polypyrrole-co-polycaprolactone substrate enhances neural cell growth

2013 ◽  
Vol 102 (8) ◽  
pp. 2554-2564 ◽  
Author(s):  
Hieu T. Nguyen ◽  
Shawn Sapp ◽  
Claudia Wei ◽  
Jacqueline K. Chow ◽  
Alvin Nguyen ◽  
...  
Keyword(s):  
Cell Growth ◽  
Electric Field ◽  
Neural Cell ◽  
Field Stimulation ◽  
Electric Field Stimulation

Influence of low voltage electric field stimulation on hydrogen generation from anaerobic digestion of waste activated sludge

2020 ◽  
Vol 704 ◽  
pp. 135849
Author(s):  
Ziletao Tao ◽  
Dongbo Wang ◽  
Fubing Yao ◽  
Xiaoding Huang ◽  
You Wu ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Electric Field ◽  
Activated Sludge ◽  
Hydrogen Generation ◽  
Low Voltage ◽  
Waste Activated Sludge ◽  
Field Stimulation ◽  
Electric Field Stimulation

scholarly journals Vimentin filaments drive migratory persistence in polyploidal cancer cells

2020 ◽  
Vol 117 (43) ◽  
pp. 26756-26765
Author(s):  
Botai Xuan ◽  
Deepraj Ghosh ◽  
Joy Jiang ◽  
Rachelle Shao ◽  
Michelle R. Dawson
Keyword(s):  
Cancer Cells ◽  
Cancer Progression ◽  
Structural Integrity ◽  
Single Cell Analysis ◽  
Critical Role ◽  
Critical Function ◽  
Rna Knockdown ◽  
Interfering Rna ◽  
The Impact

Polyploidal giant cancer cells (PGCCs) are multinucleated chemoresistant cancer cells found in heterogeneous solid tumors. Due in part to their apparent dormancy, the effect of PGCCs on cancer progression has remained largely unstudied. Recent studies have highlighted the critical role of PGCCs as aggressive and chemoresistant cancer cells, as well as their ability to undergo amitotic budding to escape dormancy. Our recent study demonstrated the unique biophysical properties of PGCCs, as well as their unusual migratory persistence. Here we unveil the critical function of vimentin intermediate filaments (VIFs) in maintaining the structural integrity of PGCCs and enhancing their migratory persistence. We performed in-depth single-cell analysis to examine the distribution of VIFs and their role in migratory persistence. We found that PGCCs rely heavily on their uniquely distributed and polarized VIF network to enhance their transition from a jammed to an unjammed state to allow for directional migration. Both the inhibition of VIFs with acrylamide and small interfering RNA knockdown of vimentin significantly decreased PGCC migration and resulted in a loss of PGCC volume. Because PGCCs rely on their VIF network to direct migration and to maintain their enlarged morphology, targeting vimentin or vimentin cross-linking proteins could provide a therapeutic approach to mitigate the impact of these chemoresistant cells in cancer progression and to improve patient outcomes with chemotherapy.

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