scholarly journals Terpenoid components, antioxidant and antibacterial activities of essential oils from fresh immature and mature leaves of Blumea balsamifera, extracted with different hydrodistillation periods

2021 ◽  
Author(s):  
Sirinapha Jirakitticharoen ◽  
Wudtichai Wisuitiprot ◽  
Pongphen Jitareerat ◽  
Chalermchai Wongs-Aree
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Antibacterial Activities ◽  
Leaf Extracts ◽  
Mature Leaves ◽  
Antioxidant Capacities

AbstractVolatiles and antioxidant capacities in essential oils (EOs) from fresh immature and mature leaves of Blumea balsamifera, extracted with different extraction periods of hydrodistillation, were investigated. There were seven major terpenoid compounds in the leaf extracts, including 2 monoterpenes of camphor and L-borneol, and 5 sesquiterpenes of silphiperfol-5-ene, 7-epi-silphiperfol-5-ene, ß-caryophyllene, ɤ-eudesmol, and α-eudesmol. Different hydrodistillation periods resulted in different quantitates and compositions of the terpenoids in EOs. The yield of EOs from the immature leaves was 1.4 times higher than the mature ones, whereas 73% of the yield was collected from the first 6 h of hydrodistillation. Camphor and L-borneol were almost collected in the first 6 h, while ß-caryophyllene, silphiperfolene, and 7-epi-silphiperfolene were above 80%, but ɤ-eudesmol and α-eudesmol were only 32 and 54% released. ß-Caryophyllene, ɤ-eudesmol, and α-eudesmol were found higher in the mature leaf EOs. Antioxidant capacities in EOs were positively related to terpenoid contents. Antibacterial activity of EOs from the immature leaves was subsequently tested. Although EOs from the hydrodistillation period of 12-18 h contained fewer terpenoid compositions, it showed the same minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) on Escherichia coli and Pseudomonas aeruginosa, compared to 0-6 h EOs.

scholarly journals Antimicrobial Susceptibility ofEscherichia coliStrains Isolated fromAlouattaspp. Feces to Essential Oils

2016 ◽  
Vol 2016 ◽  
pp. 1-4 ◽  
Author(s):  
Valéria Maria Lara ◽  
Adriano Bonfim Carregaro ◽  
Deise Flores Santurio ◽  
Mariangela Facco de Sá ◽  
Janio Moraes Santurio ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Thymus Vulgaris ◽  
Broth Microdilution ◽  
Eucalyptus Citriodora ◽  
Cymbopogon Citratus

This study evaluated thein vitroantibacterial activity of essential oils fromLippia graveolens(Mexican oregano),Origanum vulgaris(oregano),Thymus vulgaris(thyme),Rosmarinus officinalis(rosemary),Cymbopogon nardus(citronella),Cymbopogon citratus(lemongrass), andEucalyptus citriodora(eucalyptus) againstEscherichia coli(n=22) strains isolated fromAlouattaspp. feces. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined for each isolate using the broth microdilution technique. Essential oils of Mexican oregano (MIC mean = 1818 μg mL−1; MBC mean = 2618 μg mL−1), thyme (MIC mean = 2618 μg mL−1; MBC mean = 2909 μg mL−1), and oregano (MIC mean = 3418 μg mL−1; MBC mean = 4800 μg mL−1) showed the best antibacterial activity, while essential oils of eucalyptus, rosemary, citronella, and lemongrass displayed no antibacterial activity at concentrations greater than or equal to 6400 μg mL−1. Our results confirm the antimicrobial potential of some essential oils, which deserve further research.

Antibacterial Activities of Sirih Merah (Piper crocatum) Leaf Extracts

2019 ◽  
Vol 5 (3) ◽  
pp. 1-10
Author(s):  
Puspa Julistia Puspita ◽  
Mega Safithri ◽  
Nirmala Peni Sugiharti
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Agent ◽  
Leaf Extract ◽  
Antibacterial Activities ◽  
Leaf Extracts ◽  
Herbal Plants

Piper crocatum is one of medicinal herbal plants with a large number of benefits. Usually herbal plants have activity as antibacterial agent. Therefore, the objectives of this research were to obtain information on antibacterial activities of the leaf extracts of Piper crocatum againts four types of bacteria, in that Staphylococcus, Bacillus substilis, Escherichia coli, and Pseudomonas aeruginosa and then to analyze the phytochemistry of the leaf extracts of Piper crocatum. The leaves of Piper crocatum were extracted by maceration and reflux using ethanol 30%. The assays of the antibacterial activities and phytochemistry on the extracts were carried out using the method of Maria Bintang. Results showed that the yield of the extraction using ethanol by maceration method was 20.8%. Meanwhile, using the reflux method, the yield was obtained about 26.25%. The phytochemistry analysis showed that the leaf extracts of Piper crocatum contained alkaloid, steroid and tanin. According to this study, it was found that the leaf extract of Piper crocatum can be used to inhibit the growth of B. subtilis and P. aeuruginosa, but can not inhibit the growth of E.coli and S. aureus.

Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

scholarly journals Antibacterial Activities ofGinkgo bilobaL. Leaf Extracts

2011 ◽  
Vol 11 ◽  
pp. 2237-2242 ◽  
Author(s):  
S. C. Sati ◽  
Savita Joshi
Keyword(s):  
Escherichia Coli ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Antibacterial Activities ◽  
Erwinia Chrysanthemi ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Leaf Extracts ◽  
Hexane Extracts ◽  
Broth Dilution

The antibacterial activity of methanol, ethanol, chloroform, and hexane extracts of the leaves of Himalayan gymnospermous plantGinkgo bilobaL. was assessed against five animal and plant pathogenic strains (Agrobacterium tumefaciens, Bacillus subtilis, Escherichia coli, Erwinia chrysanthemi, and Xanthomonas phaseoli) employing disc-diffusion and broth-dilution assays. The methanol extract showed the highest activity (zone of inhibition of 15–21 mm) followed by ethanol (14–19 mm), chloroform (15–20 mm), and hexane (14–19 mm) extracts at 250 μg/mL. A minimum inhibitory concentration (MIC) of 7.8 μg/mL was found for the methanol extract against most of the pathogens tested.

Antibacterial Activity and Chemical Composition of the Essential Oil of Grammosciadium platycarpum Boiss. from Iran

2005 ◽  
Vol 60 (1-2) ◽  
pp. 30-34 ◽  
Author(s):  
Ali Sonboli ◽  
Fereshteh Eftekhar ◽  
Morteza Yousefzadi ◽  
Mohammad Reza Kanani
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Antibacterial Activities ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Two Samples

The chemical composition of the essential oils obtained from two samples (GP1 and GP2) of Grammosciadium platycarpum Boiss. was analyzed by GC and GC-MS. The analysis of the oils resulted in the identification of twenty-two constituents. Linalool (79.0% - GP1, 81.8% - GP2) and limonene (10.0%, 5.8%) were found to be the major components, respectively. The in vitro antibacterial activities of these oils and their main compounds against seven Gram-positive and Gram-negative bacteria were investigated. The results exhibited that the total oils and their major components possess strong to moderate activities against all the tested bacteria except for Pseudomonas aeruginosa.

scholarly journals Antibacterial and Hemolytic Activity of Crotalus triseriatus and Crotalus ravus Venom

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 281
Author(s):  
Adrian Zaragoza-Bastida ◽  
Saudy Consepcion Flores-Aguilar ◽  
Liliana Mireya Aguilar-Castro ◽  
Ana Lizet Morales-Ubaldo ◽  
Benjamín Valladares-Carranza ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Hemolytic Activity ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
The Other ◽  
Medical Treatments ◽  
First Time

Rattlesnakes have venoms with a complex toxin mixture comprised of polypeptides and proteins. Previous studies have shown that some of these polypeptides are of high value for the development of new medical treatments. The aim of the present study is to evaluate, in vitro, the antibacterial and hemolytic activity of Crotalus triseriatus and Crotalus ravus venoms. A direct field search was conducted to obtain Crotalus triseriatus and Crotalus ravus venom samples. These were evaluated to determine their antibacterial activity against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa through the techniques of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Hemolytic activity was also determined. Antibacterial activity was determined for treatments (Crotalus triseriatus 2) CT2 and (Crotalus ravus 3) CR3, obtaining a Minimum Inhibitory Concentration of 50 µg/mL and a Minimum Bactericidal Concentration of 100 µg/mL against Pseudomonas aeruginosa. CT1 (Crotalus triseriatus 1), CT2, and CR3 presented hemolytic activity; on the other hand, Crotalus ravus 4 (CR4) did not show hemolytic activity. The results of the present study indicate for the first time that Crotalus triseriatus and Crotalus ravus venoms contain some bioactive compounds with bactericidal activity against Pseudomonas aeruginosa which could be used as alternative treatment in diseases caused by this pathogenic bacterium.

Synthesis, resolution, derivatization and antibacterial activity of chuangxinmycin

2019 ◽  
Vol 11 (22) ◽  
pp. 2877-2890
Author(s):  
Lianqi Sun ◽  
Shuo Zhang ◽  
Xinyue Hu ◽  
Jie Jin ◽  
Zhuorong Li
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Antibacterial Activities ◽  
Electron Cloud ◽  
Preparative Chromatography ◽  
Novel Synthesis ◽  
Novel Structures ◽  
And Staphylococcus Aureus ◽  
Cloud Density

Aim: An urgent need for the development of antibiotics with novel structures and unexploited targets. Materials & methods: Racemic chuangxinmycin was obtained via a novel synthesis route. Chiral preparative chromatography was used to separate chuangxinmycin from its epimers, and four stereoisomers were obtained. Fourteen derivatives were synthesized and their antibacterial activities were evaluated against Escherichia coli and Staphylococcus aureus. Results: Synthesized (3 S, 4 R)-chuangxinmycin showed antibacterial activity against S. aureus with minimum inhibitory concentration of 4–8 μg/ml (17.2–34.3 μM), which were consistent with the antibacterial activity of chuangxinmycin obtained by fermentation. The minimum inhibitory concentrations of other stereoscopic chuangxinmycin species and chuangxinmycin derivatives were >128 μg/ml. Conclusion: Results indicate that the antibacterial activity of chuangxinmycin is dependent on the stereoselectivity of structures, and that the electron cloud density and amphipathic properties of chuangxinmycin have little effect on its antibacterial activity.

Serum Inhibitory and Bactericidal Activity of Ciprofloxacin following Intravenous Administration

DICP ◽  
1989 ◽  
Vol 23 (6) ◽  
pp. 456-460
Author(s):  
Michael N. Dudley ◽  
Hilary D. Mandler ◽  
Kenneth H. Mayer ◽  
Stephen H. Zinner
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Minimum Inhibitory Concentration ◽  
Bactericidal Activity ◽  
Resistant Strain ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
E Coli ◽  
Dose Levels

Serum inhibitory and bactericidal titers were measured in nine healthy volunteers following single iv doses of ciprofloxacin 100, 150, and 200 mg. The median peak serum bactericidal titer (5 minutes following completion of a 30-minute infusion) against two highly susceptible strains of Escherichia coli ranged between 1:64 and 1:1024 and titers exceeded 1:8 for six hours for all dose levels. The bactericidal titers against two strains of Pseudomonas aeruginosa and a methicillin-resistant strain of Staphylococcus aureus were considerably lower, the median peak being 1:2 at all dose levels. Measured inhibitory and bactericidal titers at five minutes and one hour postinfusion were significantly greater than those predicted (measured serum ciprofloxacin concentration to minimum inhibitory concentration [MIC] or minimum bactericidal concentration [MBC]) for only one strain of E. coli. Intravenous doses of ciprofloxacin 100–200 mg produce high and sustained serum bactericidal titers against highly susceptible bacteria; considerably lower levels of activity are seen against bacteria having higher MICs and MBCs but still considered susceptible to the drug.

scholarly journals Antibacterial Activity and Composition of Essential Oils from Flower, Leaf and Stem of Chaerophyllum Macropodum Boiss. from Iran

2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Ali Shafaghat
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Essential Oils ◽  
Antibacterial Activities ◽  
Salmonella Typhi ◽  
Inhibitory Effects ◽  
Leaf Oil ◽  
Main Components ◽  
Microdilution Broth

The hydrodistilled essential oils from flower, leaf and stem of Chaerophyllum macropodum Boiss. were analyzed by GC and GC/MS. The flower oil was characterized by high amounts of myristicin (42.5%) and trans-β-ocimene (41.0%). In the leaf oil eighteen constituents, representing 99.3% of the total, were identified of which trans-β-ocimene (24.9%), myristicin (15.7%), terpinolene (14.5%), fenchyl acetate (13.9%), cis-β-ocimene (6.3%) and sabinene (6.1%) were major components. The main components of the stem oil were trans-β-ocimene (54.2%), myristicin (22.4%) and sabinene (8.9%). Antibacterial activities of the flower, leaf and stem oils were evaluated using the microdilution broth method. Inhibitory effects on Escherichia coli and Salmonella typhi were recorded.

scholarly journals Antibacterial Activity of Solanum surattense Burm. F

1970 ◽  
Vol 6 (1) ◽  
pp. 1-4 ◽  
Author(s):  
E Sheeba
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Salmonella Typhi ◽  
Shigella Dysenteriae ◽  
Leaf Extracts ◽  
Solanum Surattense ◽  
Engineering And Technology ◽  
Ethanol Extracts

Ethanol extracts of Solanum surattense used in traditional medicine for treatmentof various infections. The antibacterial activity detected was againstStaphylococcus aureus, Streptococcus sp.; Bacillus subtilis, Escherichia coli,Pseudomonas aeruginosa, Salmonella typhi, Shigella dysenteriae and Vibriocholerae. The highest antibacterial activity was observed in 500μg concentrationof leaf extracts of all bacteria screened except Shigella dysenteriae. The minimumzone of inhibition observed in 25μg concentration of leaf extract exceptPseudomonas aeruginosa and Shigella dysenteriae. These results indicate that theextracts were bacteriostatic at higher concentrations.Keywords: Antibacterial activity; Solanum surattense; pathogenicmicro organisms.DOI: 10.3126/kuset.v6i1.3278Kathmandu University Journal of Science, Engineering and Technology Vol.6(1) 2010, pp 1-4

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