scholarly journals Timing of meristem initiation and maintenance determines the morphology of fern gametophytes

2021 ◽  
Author(s):  
Xiao Wu ◽  
An Yan ◽  
Scott McAdam ◽  
Jo Ann Banks ◽  
Shaoling Zhang ◽  
...  
Keyword(s):  
Vascular Plants ◽  
Plant Architecture ◽  
Time Lapse ◽  
Seed Plants ◽  
Cellular Mechanisms ◽  
Free Living ◽  
Meristem Development ◽  
Insight Into ◽  
Fern Gametophytes ◽  
Alternation Of Generations

The alternation of generations in land plants occurs between the sporophyte phase and the gametophyte phase. The sporophytes of seed plants develop self-maintained, multicellular meristems, and these meristems determine plant architecture. The gametophytes of seed plants lack meristems and are heterotrophic. In contrast, the gametophytes of seed-free vascular plants, including ferns, are autotrophic and free-living, developing meristems to sustain their independent growth and proliferation. Compared to meristems in the sporophytes of seed plants, the cellular mechanisms underlying meristem development in fern gametophytes remain largely unknown. Here, using confocal time-lapse live imaging and computational segmentation and quantification, we determined different patterns of cell divisions associated with the initiation and proliferation of two distinct types of meristems in fern gametophytes. Our results reveal how the simple timing of a switch between two meristems has considerable consequences for the divergent gametophyte morphologies of two closely related ferns from Pteridaceae (Pteris and Ceratopteris). Our result provides evolutionary insight into the function and regulation of gametophyte meristems in seed-free vascular plants.

Ultrastructure of neuromuscular relationships in the canine heartworm (dirofilaria immitis)

1986 ◽  
Vol 44 ◽  
pp. 278-279
Author(s):  
W. L. Steffens ◽  
Nancy B. Roberts ◽  
J. M. Bowen
Keyword(s):  
Dirofilaria Immitis ◽  
Domestic Dogs ◽  
Structural Description ◽  
Pharmacological Effects ◽  
Free Living ◽  
Canine Heartworm ◽  
The World ◽  
Synaptic Region ◽  
Muscle Innervation ◽  
Insight Into

The canine heartworm is a common and serious nematode parasite of domestic dogs in many parts of the world. Although nematode neuroanatomy is fairly well documented, the emphasis has been on sensory anatomy and primarily in free-living soil species and ascarids. Lee and Miller reported on the muscular anatomy in the heartworm, but provided little insight into the peripheral nervous system or myoneural relationships. The classical fine-structural description of nematode muscle innervation is Rosenbluth's earlier work in Ascaris. Since the pharmacological effects of some nematacides currently being developed are neuromuscular in nature, a better understanding of heartworm myoneural anatomy, particularly in reference to the synaptic region is warranted.

scholarly journals Significance of Mast Cell Formed Extracellular Traps in Microbial Defense

2021 ◽  
Author(s):  
Daniel Elieh Ali Komi ◽  
Wolfgang M. Kuebler
Keyword(s):  
State Of The Art ◽  
Extracellular Dna ◽  
Cellular Mechanisms ◽  
Extracellular Traps ◽  
Synthetic Chemicals ◽  
Dna Strands ◽  
Associated Proteins ◽  
Microbial Defense ◽  
And Function ◽  
Insight Into

AbstractMast cells (MCs) are critically involved in microbial defense by releasing antimicrobial peptides (such as cathelicidin LL-37 and defensins) and phagocytosis of microbes. In past years, it has become evident that in addition MCs may eliminate invading pathogens by ejection of web-like structures of DNA strands embedded with proteins known together as extracellular traps (ETs). Upon stimulation of resting MCs with various microorganisms, their products (including superantigens and toxins), or synthetic chemicals, MCs become activated and enter into a multistage process that includes disintegration of the nuclear membrane, release of chromatin into the cytoplasm, adhesion of cytoplasmic granules on the emerging DNA web, and ejection of the complex into the extracellular space. This so-called ETosis is often associated with cell death of the producing MC, and the type of stimulus potentially determines the ratio of surviving vs. killed MCs. Comparison of different microorganisms with specific elimination characteristics such as S pyogenes (eliminated by MCs only through extracellular mechanisms), S aureus (removed by phagocytosis), fungi, and parasites has revealed important aspects of MC extracellular trap (MCET) biology. Molecular studies identified that the formation of MCET depends on NADPH oxidase-generated reactive oxygen species (ROS). In this review, we summarize the present state-of-the-art on the biological relevance of MCETosis, and its underlying molecular and cellular mechanisms. We also provide an overview over the techniques used to study the structure and function of MCETs, including electron microscopy and fluorescence microscopy using specific monoclonal antibodies (mAbs) to detect MCET-associated proteins such as tryptase and histones, and cell-impermeant DNA dyes for labeling of extracellular DNA. Comparing the type and biofunction of further MCET decorating proteins with ETs produced by other immune cells may help provide a better insight into MCET biology in the pathogenesis of autoimmune and inflammatory disorders as well as microbial defense.

scholarly journals Bichir external gills arise via heterochronic shift that accelerates hyoid arch development

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Jan Stundl ◽  
Anna Pospisilova ◽  
David Jandzik ◽  
Peter Fabian ◽  
Barbora Dobiasova ◽  
...  
Keyword(s):  
Pharyngeal Arch ◽  
Developmental Sequence ◽  
Free Living ◽  
Pharyngeal Arches ◽  
Hyoid Arch ◽  
Early Appearance ◽  
Evolutionary Changes ◽  
Insight Into ◽  
Living Group ◽  
Early Larvae

In most vertebrates, pharyngeal arches form in a stereotypic anterior-to-posterior progression. To gain insight into the mechanisms underlying evolutionary changes in pharyngeal arch development, here we investigate embryos and larvae of bichirs. Bichirs represent the earliest diverged living group of ray-finned fishes, and possess intriguing traits otherwise typical for lobe-finned fishes such as ventral paired lungs and larval external gills. In bichir embryos, we find that the anteroposterior way of formation of cranial segments is modified by the unique acceleration of the entire hyoid arch segment, with earlier and orchestrated development of the endodermal, mesodermal, and neural crest tissues. This major heterochronic shift in the anteroposterior developmental sequence enables early appearance of the external gills that represent key breathing organs of bichir free-living embryos and early larvae. Bichirs thus stay as unique models for understanding developmental mechanisms facilitating increased breathing capacity.

scholarly journals HARBOR STUDY FOR SAN NICOLAS BAY, PERU

1970 ◽  
Vol 1 (12) ◽  
pp. 133
Author(s):  
James M. Keith ◽  
Emmett J. Murphy
Keyword(s):  
Particle Velocity ◽  
Time Lapse ◽  
Wave Action ◽  
Ship Motion ◽  
Long Period ◽  
Wave Property ◽  
Directional Wave ◽  
Detailed Interpretation ◽  
Wave Phenomena ◽  
Insight Into

A pair of ducted impeller current meters, one mounted vertically and the other horizontally, were used to measure wave action at San Nicolas Harbor, Peru The horizontal water velocity records are superior to conventional wave records because they measure directly the wave property which induces adverse horizontal ship motion, and provide directional wave data Spectral analysis methods proved well-suited to detailed interpretation of the particle velocity records, while considerable insight into the wave phenomena was gained by simple, rational inspections and interpretations of the records Time-lapse movies of a moored ship, when correlated with simultaneous water particle velocity records, provided an exceptionally clear picture of ship response to wave action, and led to the rather surprising observation that long-period ship motion is not necessarily caused by long-period waves The foregoing ship response was duplicated in hydrau1ic model tests.

scholarly journals A family of T6SS antibacterial effectors related to L,D-transpeptidases targets the Peptidoglycan

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Stephanie Sibinelli de Sousa ◽  
Julia Takuno Hespanhol ◽  
Bruno Matsuyama ◽  
Stephane Mesnage ◽  
Gianlucca Nicastro ◽  
...  
Keyword(s):  
Cell Envelope ◽  
Point Mutations ◽  
Time Lapse ◽  
Secretion Systems ◽  
Bacterial Antagonism ◽  
New Family ◽  
Type Vi Secretion ◽  
Altered Cell ◽  
Bacterial Effectors ◽  
Insight Into

Type VI secretion systems (T6SSs) are contractile nanomachines widely used by bacteria to intoxicate competitors. Salmonella Typhimurium encodes a T6SS within the Salmonella pathogenicity island 6 (SPI-6) that is used during competition against species of the gut microbiota. We characterized a new SPI-6 T6SS antibacterial effector named Tlde1 (type VI L,D-transpeptidase effector 1). Tlde1 is toxic in target-cell periplasm and its toxicity is neutralized by co-expression with immunity protein Tldi1 (type VI L,D-transpeptidase immunity 1). Time-lapse microscopy revealed that intoxicated cells display altered cell division and lose cell envelope integrity. Bioinformatics analysis showed that Tlde1 is evolutionarily related to L,D-transpeptidases. Point mutations on conserved histidine121 and cysteine131 residues eliminated toxicity. Co-incubation of purified recombinant Tlde1 and peptidoglycan tetrapeptides showed that Tlde1 displays both L,D-carboxypeptidase activity by cleaving GM-tetrapeptides between meso-diaminopimelic acid3 and D-alanine4, and L,D-transpeptidase exchange activity by replacing D-alanine4 for a non-canonical D-amino acid. Tlde1 constitutes a new family of T6SS effectors widespread in Proteobacteria. This work increases our knowledge about the bacterial effectors used in interbacterial competitions and provides molecular insight into a new mechanism of bacterial antagonism.

scholarly journals Allosteric deregulation of phenylalanine biosynthesis evolved with the emergence of vascular plants

2019 ◽  
Author(s):  
Jorge El-Azaz ◽  
Francisco M. Cánovas ◽  
Belén Barcelona ◽  
Concepción Ávila ◽  
Fernando de la Torre
Keyword(s):  
Vascular Plants ◽  
Seed Plants ◽  
Type I ◽  
Type Ii ◽  
Adjacent Region ◽  
In Planta ◽  
Secondary Products ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Rate Limiting

AbstractPhenylalanine (Phe) is the precursor of essential secondary products in plants. Here we show that a key, rate-limiting step in Phe biosynthesis, which is catalyzed by arogenate dehydratase (ADT), experienced allosteric de-regulation during evolution. Enzymes from microorganisms and type-I ADTs from plants are strongly feedback-inhibited by Phe, while type-II isoforms remain active at high levels of Phe. We have found that type-II ADTs are widespread across seed plants and their overproduction resulted in a dramatic accumulation of Phe in planta, up to 40-times higher than those observed following the expression of type-I enzymes. Punctual changes in the allosteric binding site of Phe and adjacent region are responsible for the observed relaxed regulation. The phylogeny of plant ADTs evidences that the emergence of type-II isoforms with relaxed regulation occurred at some point in the transition between non-vascular plants and tracheophytes enabling the massive production of Phe-derived compounds, primarily lignin, which are attributes of vascular plants.

Cell behaviour in a polygonal cell sheet*

Development ◽  
1984 ◽  
Vol 83 (Supplement) ◽  
pp. 313-327
Author(s):  
H. Honda ◽  
R. Kodama ◽  
T. Takeuchi ◽  
H. Yamanaka ◽  
K. Watanabe ◽  
...  
Keyword(s):  
Cell Sheet ◽  
Cell Monolayer ◽  
Time Lapse ◽  
Geometrical Method ◽  
Cell Behaviour ◽  
Cell Monolayers ◽  
Polygonal Cell ◽  
Computer Based ◽  
Insight Into

Cell monolayers on culture dishes were divided into two groups: tensile monolayers and non-tensile ones. In the development of an epithelium, a non-tensile cell monolayer turns into a tightly bound tensile one. Detection of these states was carried out by using the boundary shortening procedure, a computer-based geometrical method to show how much the polygonal cell boundary contracts. Non-tensile monolayers were divided further into two groups according to their motility: a fluctuating monolayer in which cells move laterally, and a stable monolayer in which cells are immobilized. Quantitative determination of cell motility was performed by analysing time-lapse cellular patterns. These computer-based geometrical analyses enabled us to divide monolayers into three groups: tensile stable monolayers, non-tensile stable monolayers and fluctuating monolayers, and this study therefore gives an insight into the way in which changing conformations of cells may be assayed.

Biodiversity of intertidal marine flatworms (Polycladida, Platyhelminthes) in southeastern Australia

Zootaxa ◽  
2021 ◽  
Vol 5024 (1) ◽  
pp. 1-63
Author(s):  
JORGE RODRÍGUEZ ◽  
PAT A. HUTCHINGS ◽  
JANE E. WILLIAMSON
Keyword(s):  
Dna Sequences ◽  
New South ◽  
Marine Biota ◽  
Free Living ◽  
Marine Fauna ◽  
Southeastern Australia ◽  
Marine Habitats ◽  
South Wales ◽  
Molecular Sequencing ◽  
Insight Into

Flatworms of the Order Polycladida are a group of free-living invertebrates found in a diversity of marine habitats, with over 800 species described worldwide. Marine flatworms are a conspicuous component of Australia’s marine fauna yet have received little attention. Less than 30 scientific articles have been published on Australian marine flatworms since 1855, of which only nine include species from southeastern Australia. Here, the biodiversity and distribution of species belonging to the Order Polycladida inhabiting intertidal rocky beaches in southeastern Australian waters were identified and analysed. Sampling was conducted at low tide along the coasts of New South Wales and Victoria. Collected samples were serially sectioned for comparative anatomical studies, and tissue was removed from each individual for molecular sequencing and analyses. Both nuclear and mitochondrial DNA sequences were obtained and used as an additional source of evidence for the description of new species as well as providing further insight into the phylogenetic relationships between them. A total of 20 species, six of which are new (e.g., Eulatocestus australis sp. nov.), and a new genus (Parabolia gen. nov.) have been described, as well as two new records for Australia (e.g., Stylochoplana clara Kato, 1937) have been identified increasing our knowledge of this important component of the Australian marine biota.  

Sign in / Sign up

Export Citation Format

Share Document