Genotoxicity evaluation of levonorgestrel-releasing intrauterine system (LNG-IUS) in exfoliated cervical cells using the micronucleus (MN) test

Purpose: This study aimed to determine whether any relationships exist between the levonorgestrel-releasing intrauterine system (LNG-IUS) and micronuclei or other nuclear anomalies, including condensed chromatin, karyorrhexis, and karyolysis, on the cervical epithelium in young women. Methods: A prospective observational study was conducted. The study population comprised healthy women aged ≤40 years who were referred for birth control. Cervical smears that were obtained from 18 women before and three months after LNG-IUS insertion were tested for micronuclei and other nuclear anomaliesusing the micronucleus test. Results: The results revealed no statistically significant difference (P>0.05) in the frequency of micronucleated exfoliated cervical mucosa cells after LNG-IUS exposure. However, LNG-IUS was able to increase other nuclear alterations closely related to cytotoxicity. Conclusions: Data indicated that exposure to LNG-IUS may not be a factor in inducing chromosomal damage, but it can promote cytotoxicity.

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ANALYSIS OF GENOTOXICITY IN ERITHROCYTES OF TURTLES (Phrynops hilarii) FROM ANTHROPIZED AND NATURAL SITES OF ENTRE RÍOS, ARGENTINA

Journal of Basic and Applied Genetics ◽

10.35407/bag.2020.31.01.02 ◽

2020 ◽

Vol 31 (1) ◽

pp. 15-22

Author(s):

G. V. Castaño ◽

M. Cabagna Zenklusen ◽

Y. Prieto ◽

A. S. Manzano

Keyword(s):

Micronucleus Test ◽

Femoral Vein ◽

Sampling Site ◽

Natural Populations ◽

Urban System ◽

Bioindicator Species ◽

Significant Difference ◽

Nuclear Alterations ◽

Entre Rios ◽

Non Destructive

The micronucleus test (MN) is a biomarker of non-destructive genotoxicity that allows chromosomal damage and other nuclear alterations (NA) to be detected. Phrynops hilarii is a freshwater chelonium that inhabits regions of central-northern Argentina. The main objective was to determine the presence of MN and other NA in erythrocytes of natural populations of P. hilarii comparing their frequencies between three sites, two anthropized and one of control (cities of Diamante and Paraná) of Entre Ríos, Argentina, during the period 2015-2016. Eighteen individuals (six per sampling site) were evaluated at the sites: 1- PD: Pre-Delta National Park (control), 2- AG: Salto Ander Egg (agroecosystem) and 3- SU: Caleta Club Náutico (urban system). Blood was obtained from the femoral vein. The samples were stained with the May Grünwald-Giemsa method and observed under a microscope with an immersion objective. Micronucleus (MNF) and nuclear alterations (NAF) frequencies were determined every 1000 erythrocytes observed. A significant difference (p<0.05) was found between the PD site and the other sites (AG and SU), both for MNF (p=0.0021) and for NAF (p=0.0011). The highest frequency values corresponded to the AG site (MNF: 3.33 ± 0.62; NAF: 4.67 ± 0.56). Finally, biomonitoring with P. hilarii was useful, so it could be considered as a bioindicator species to assess the quality of Argentina’s environments. Key words: Genotoxicity biomarkers, micronucleus test, nuclear alterations

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PEMANFAATAN FENOMENA ALAM GUNUNG GALUNGGUNG SEBAGAI SUMBER BELAJAR UNTUK MENGEMBANGKAN DAYA ANALISIS MAHASISWA GEOGRAFI

NATURALISTIC : Jurnal Kajian Penelitian Pendidikan dan Pembelajaran ◽

10.35568/naturalistic.v1i2.6 ◽

2017 ◽

Vol 1 (2) ◽

pp. 186-192

Author(s):

Erwin Hilman Hakim

Keyword(s):

Power Analysis ◽

Field Studies ◽

Statistical Techniques ◽

Test Results ◽

Significant Difference ◽

Depth Analysis ◽

Quantitative Data Analysis ◽

Study Population ◽

Faculty Of Education ◽

Range Of Values

Natural phenomena Mountain Galunggung learning resources in understanding the symptoms of volcanism, these phenomena can be used as a source of learning geography student to improve the analysis of their environment. Geography is a natural laboratory and a lot of geography materials that can not be understood simply by studying a concept in the literature book is done in space by verbalistis. This research was conducted at the Department of Geography Students of the Faculty of Education Siliwangi University. This study population Geography students the semester II of classes using model experiments with field studies and grade control using model lecture. Quantitative data analysis techniques using statistical methods, there are two statistical techniques used, namely descriptive statistics for the description of research data and statistical techniques t-test. There is a significant difference to the results of the test and development of power analysis geography student this is evidenced by the results of tests experimental minimum 13, maximum numbers 23, the range of values 10, a mean of 18.15, median 18.00. The test results control class minimum 10, maximum 20, the range of values 10, mean 15.39, median 15.00. Power analysis geography student experiment class has a depth analysis of the phenomena that is the result of data processing that students can open up and develop the power of analysis, the material is easy to understand, the students more active and interactive, while for the control class learning process is less effective without a concrete example to be analytical.

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An Investigation of the Attitudes of Health & Safety Personnel toward Safety in Construction Projects

Archives of Occupational Health ◽

10.18502/aoh.v3i1.348 ◽

2019 ◽

Author(s):

Ahmad Ghorbani ◽

Ahmad Soltanzadeh

Keyword(s):

Factor Analysis ◽

Work Experience ◽

Pearson Correlation ◽

Correlation Coefficients ◽

T Test ◽

Test Results ◽

Entire Study ◽

Safety Attitudes ◽

Significant Difference ◽

Study Population

Background and objective: With the development of technology and its increased use, potential dangers in industrial environments has increased. The purpose of this study was to evaluate the attitudes of the Fajr Institute's Health & Safety Executive (HSE) personnel toward safety. Method: The study population consisted of HSE staff (n: 39). Data gathering tool in this descriptive-analytical study was a researcher-developed, 30-item questionnaire with acceptable validity (1.9) and reliability (0.87). Data were analyzed using the SPSS 19. Samples were selected by census sampling and the entire study population was studied. Statistical methods used were mean, variance, standard deviation, t-test, Pearson's correlation coefficient, and factor analysis. Result: The average age of the participants was 28.6 years, 56.5% of them were single and 43.5% married. The average work experience of the participants was 5.2 years. Bachelor's degree (54%) and Master's degree (23%) were the most and least frequent academic degrees, respectively. The Pearson correlation coefficients showed age and work experience were not correlated with safety attitudes. The t-test results showed there was a significant difference between the viewpoints of single and married workers and attitude variable (P<0.01).The t-test results also showed there was no significant difference between education level and attitude. Conclusion: According to the factor analysis results, items were classified into four categories: management factors, educational factors, communication factors, and regulatory factors.

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Allergic Rhinitis and its Impact on Asthma classification correlations

The Journal of Laryngology & Otology ◽

10.1017/s0022215114000693 ◽

2014 ◽

Vol 128 (5) ◽

pp. 431-437 ◽

Cited By ~ 6

Author(s):

H Baran ◽

K M Ozcan ◽

A Selcuk ◽

M A Cetin ◽

S Cayir ◽

...

Keyword(s):

Allergic Rhinitis ◽

Symptom Severity ◽

Test Results ◽

House Dust Mite Allergy ◽

Prick Test ◽

Mite Allergy ◽

Significant Difference ◽

Symptom Frequency ◽

Study Population

AbstractObjective:Allergic rhinitis is inflammation of the nasal mucosa following exposure to allergens. A few studies have investigated how the Allergic Rhinitis and its Impact on Asthma classification correlates with symptom severity and skin prick test results in allergic rhinitis patients. Our objective was to evaluate such a correlation.Materials and methods:The study population consisted of 268 patients who had allergic symptoms and were diagnosed with allergic rhinitis.Results:Analysis of the Allergic Rhinitis and its Impact on Asthma classification with regard to symptom severity revealed that there were statistically significant differences between the groups for nasal symptoms, wheezing, eye itching/watering and eye redness. Symptom frequency and severity increased gradually towards the moderate to severe persistent allergic group. There was a statistically significant difference between the groups for grass pollen and house dust mite allergy.Conclusion:The Allergic Rhinitis and its Impact on Asthma classification, which takes symptoms and quality of life into consideration in addition to allergen exposure, is a useful classification system for allergic rhinitis.

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Mutagenicity and cytotoxicity in patients submitted to ionizing radiation

The Angle Orthodontist ◽

10.2319/013112-88.1 ◽

2012 ◽

Vol 83 (1) ◽

pp. 104-109 ◽

Cited By ~ 17

Author(s):

Diego Coelho Lorenzoni ◽

Ana Carolina Cuzzuol Fracalossi ◽

Viviane Carlin ◽

Daniel Araki Ribeiro ◽

Eduardo Franzotti Sant'Anna

Keyword(s):

Buccal Mucosa ◽

Micronucleus Test ◽

Mutagenic Effect ◽

Cone Beam ◽

Healthy Children ◽

Conventional Radiograph ◽

Buccal Mucosa Cells ◽

Significant Difference ◽

Before And After ◽

Nuclear Alterations

Abstract Objectives: To evaluate and compare mutagenicity (micronucleus) and cytotoxicity (karyorrhexis, pyknosis, and karyolysis) in exfoliated buccal mucosa cells of children following cone beam computed tomography (CBCT) or conventional radiograph exposure necessary for orthodontic planning. Materials and Methods: A total of 49 healthy children were submitted to CBCT or a conventional orthodontic radiographic protocol; they were divided into two groups based on exam: CBCT (n = 24) and Radiographic Set (n = 25) groups. The micronucleus test in the exfoliated buccal mucosa cells was applied. Results: There was not a statistically significant difference (P > .05) found between the number of micronucleated buccal mucosa cells (MNC) before and after exposure to radiation in either group, showing that neither group experienced a mutagenic effect. However, radiation did cause other nuclear alterations closely related to cytotoxicity, including karyorrhexis, pyknosis, and karyolysis, in both groups (P < .05). The CBCT group presented a greater increase in cell death than was noted in the Radiographic Set group (P < .044). Conclusion: According to the micronucleus test, mutagenicity was not induced by the CBCT or the conventional radiographs, but cytotoxicity was verified after these exams, especially after CBCT. That might have happened once the CBCT group received a greater radiation dose than the Radiographic Set group as a result of the protocols used in orthodontic planning for this study.

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Perbedaan sensitivitas tetes telinga antibiotik terhadap Pseudomonas aeruginosa pada otitis media supuratif kronik

Oto Rhino Laryngologica Indonesiana ◽

10.32637/orli.v42i2.22 ◽

2012 ◽

Vol 42 (2) ◽

Author(s):

Anton Budhi Darmawan ◽

Dwi Utami Anjarwati

Keyword(s):

Pseudomonas Aeruginosa ◽

Otitis Media ◽

Chronic Suppurative Otitis Media ◽

Test Results ◽

Sectional Study ◽

Suppurative Otitis Media ◽

Number Of Patients ◽

Significant Difference ◽

Cochran Test ◽

Post Hoc

Background: Chronic suppurative otitis media (CSOM) is one infectious disease of the middle ear, most commonly caused by Pseudomonas aeruginosa. A high number of patients come to the ENT outpatient clinic with active benign type of CSOM. The bacteria Pseudomonas aeruginosa is capable of producing biofilm which protects itself from penetration of antibiotics, and therefore creates resistance towards antibiotics and difficult to eradicate. Objective: The aim of this study was to compare the sensitivity levels of chloramphenicol, polymyxin-neomycin, cyprofloxacin and ofloxacine against Pseudomonas aeruginosa in patients with active benign type CSOM in ENT clinic. Method: The method used was across sectional study on 25 patients, from August 2010 until December 2010. Samples were taken withear swab and then put on sensitivity test to chloramphenicol, polymyxin-neomycin, cyprofloxacin andofloxacine using the diffusion disc method. The analysis used in this study was Cochran test. Results: Results showed a significant difference in sensitivity among chloramphenicol (38,70%), polymyxinneomycin(83,87%),cyprofloxacin(90,32%)andofloxacin(58,06%)withp=0,000(p<0,05).PostHocanalysisusing the Mc Nemar indicated that there were significant differences in sensitivity betweenpolymyxin-neomycin to chloramphenicol with p=0,000 (p<0,05), ciprofloxacin to chloramphenicol andciprofloxacin to ofloxacine with p= 0,002, but there were no significant differences between cyprofloxacinto polymyxin-neomycin with p=0,687, polymyxin-neomycin to ofloxacin p=0.057 and ofloxacin tochloramphenicol p=0,109. There were significant differences in antibiotic ear dropssensitivity to Pseudomonas aeruginosa in patients with active benign type of CSOM. Cyprofloxacin andpolymyxin-neomycin were more sensitive than ofloxacin and chloramphenicol. Keywords: Pseudomonas aeruginosa, active benign type of chronic suppurative otitis media, antibioticear drops. Abstrak : Latar belakang: Otitis media supuratif kronik (OMSK) merupakan penyakit infeksi kronik telinga tengah yang sering dijumpai di klinik THT. Penyebab tersering OMSK adalah bakteri Pseudomonasaeruginosa. Pseudomonas aeruginosa mempunyai kemampuan untuk membentuk biofilm yangmelindunginya dari penetrasi antibiotik sehingga menimbulkan resistensi terhadap antibiotik dan sulituntuk eradikasinya. Tujuan: Untuk mengetahui perbandingan tingkat sensitivitas kloramfenikol,polimiksin-neomisin, ciprofloksasin dan ofloksasin terhadap isolat Pseudomonas aeruginosa padapasien OMSK benigna aktif di klinik THT RSMS. Metode: Metode yang digunakan adalah crosssectional terhadap 29 pasien OMSK di klinik THT RSMS periode bulan Agustus 2010 - Desember2010. Pengambilan sampel dilakukan dengan swab telinga. Uji sensitivitas terhadap kloramfenikol,polimiksin-neomisin, ciprofloksasin dan ofloksasin dilakukan dengan metode cakram secara difusi.Analisis yang digunakan dalam penelitian ini adalah Cochran dan analisis post hoc. Hasil: Didapatkansensitivitas kloramfenikol sebesar 38,70%, polimiksin-neomisin sebesar 83,87%, ciprofloksasin sebesar90,32% dan ofloksasin sebesar 58,06% dengan p=0,01 (P<0,05), yang menunjukkan adanya perbedaansensitivitas yang bermakna antara kloramfenikol, polimiksin-neomisin, ciprofloksasin dan ofloksasinterhadap Pseudomonas aeruginosa. Analisis post hoc menggunakan Mc Nemar menunjukkan bahwaterdapat perbedaan sensitivitas yang bermakna antara tetes telinga polimiksin-neomisin terhadapkloramfenikol, dan ciprofloksasin terhadap kloramfenikol p=0,000 (p<0,05), serta terdapat perbedaanyang bermakna antara tetes telinga ciprofloksasin terhadap ofloksasin, p=0,002, tetapi tidak terdapatperbedaan yang bermakna antara ciprofloksasin terhadap polimiksin-neomisin, p=0,687, polimiksinneomisinterhadap ofloksasin p=0,057, dan kloramfenikol terhadap ofloksasin p=0,109. Kesimpulan:Terdapat perbedaan sensitivitas yang bermakna tetes telinga antibiotik terhadap Pseudomonas aeruginosa pada pasien OMSK benigna aktif. Ciprofloksasin dan polimiksin-neomisin tetes telinga mempunyai sensitivitas yang lebih baik dibanding ofloksasin dan kloramfenikol. Kata kunci: Pseudomonas aeruginosa, otitis media supuratif kronik, tetes telinga antibiotik

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A STUDY OF CONDYLAR POSITION IN CENTRIC RELATION AND MAXIMAL INTERCUSPATION USING CONEBEAM TOMOGRAPHY

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2019.2.14 ◽

2019 ◽

pp. 84-92

Author(s):

Anh Chi Phan ◽

Xuan Anh Ngoc Ho

Keyword(s):

Dental Students ◽

Cone Beam ◽

Test Results ◽

Reference Position ◽

Centric Relation ◽

Temporomandibular Joints ◽

Significant Difference ◽

Mandibular Fossa ◽

Condylar Position ◽

Cone Beam Tomography

Background: The condylar position discrepancy between centric relation and maximal intercuspation has been still a controversial issue. Aims: To compare the condylar position between centric relation and maximal intercuspation using cone-beam tomography in patients without temporomandibular joints disorder. Materials and methods: To assess the condylar position in centric relation and maximal intercuspation using cone-beam tomography on 40 fifth-year and sixth-year dental students of Hue University of Medicine and Pharmacy without temporomandibular joints disorder. The condylar positions are assessed following Sener classification (2009) and are compared between centric relation and maximal intercuspation using pairedsamples t-test and Mann-Whitney U-test. Results: Among 480 condye-to-fossa measurement pairs, there are 91.2% pairs having difference between two reference position but there is no significant difference. The condylar position at the superior of mandibular fossa has the greatest percentage in both centric relation and maximal intercuspation (43.8% in centric relation and 51.2% in maximal intercuspation). This greatest percentage is followed by the condylar position at posterior of mandibular fossa (32.5% in centric relation and 36.3% in maximal intercuspation). Lastly, the condylar position at the anterior of mandibular fossa has the fewest percentage (23.7% in centric relation and 12.5% in maximal intercuspation). Conclusion: There is no significant difference of condylar position between centric relation and maximal intercuspation in patients without temporomandibular joints disorder. Key words: Condylar position, centric relation, maximal intercuspation, cone-beam tomography

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Reducing Gingivitis Remotely using the iGAM mHealth app (part 2): Prospective Observational Study (Preprint)

10.2196/preprints.24955 ◽

2020 ◽

Author(s):

Guy Tobias ◽

Assaf B Spanier

Keyword(s):

Oral Health ◽

Tooth Loss ◽

Prospective Observational Study ◽

Dental Clinics ◽

Significant Difference ◽

Observational Cohort ◽

Group 2 ◽

And Behavior ◽

Oral Hygiene Habits ◽

Group 1

BACKGROUND Gingivitis is a non-painful, inflammatory condition that can be treated with home remedies. Left untreated gingivitis can lead to tooth loss. Periodic dental examinations are important for early diagnosis and treatment of gum diseases. In order to contain the spread of the corona virus, governments, including in Israel, have restricted movements of their citizens which has caused routine dental checkups to be postponed. OBJECTIVE This study aimed to examine the ability of an mHealth app- iGAM to reduce gingivitis. METHODS A prospective observational cohort study was performed, 160 unpaid participants were divided into 2 equal groups and downloaded the iGAM app. Group 1 photographed their gums weekly for eight weeks. Group 2 photographed their gums at the time of recruitment and 8 weeks later. After photo submission, the participants received the message "It is recommended to read the information contained within the app regarding maintaining oral hygiene habits". A single blinded researcher examined the images and scored them according to the Modified Gingival Index (MGI). RESULTS The average age of group 1 was 26.77 (S.D. ± 7.43), and 28.53 (S.D. ± 10.44) for group 2. The majority were male (74.7% in group 1 vs. 66.7% in group 2), most participants described themselves as "secular", most were "single", non-smokers (74.7% vs. 78.4%) and did not take medications (85.3% vs. 78.4%). 126 subjects completed the study. A statistically significant difference (P <.001) was found in the dependent variable (MGI) in a linearly negative manner. As time passed, the gum condition improved, there were significantly lower gingivitis scores in group 1 (M = 1.16, S.D. ± 1.18) compared to group 2 (M = 2.16, S.D. ± 1.49), after eight weeks. Those with more recent dental visits had a lower MGI (p = .037). No association was found between knowledge and behavior, most participants were familiar with the recommendations for maintaining oral health, yet they only performed some. CONCLUSIONS A dental selfie taken once a week using an mHealth app (iGAM) reduced the signs of gingivitis and promoted oral health. During the current pandemic where social distancing recommendations may be causing people to avoid dental clinics, this app can remotely promote gum health. CLINICALTRIAL The protocol was approved by Hadassah research ethics committee (IRB, 0212-18-HMO)

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Assessing the Attitude of Tampa Bay Youth toward HIV Self-Testing Kits

Tropical Medicine and Infectious Disease ◽

10.3390/tropicalmed6030111 ◽

2021 ◽

Vol 6 (3) ◽

pp. 111

Author(s):

Sherry Zhang ◽

Isabella Lopez ◽

Bernard Washington ◽

Brittney Gaudet ◽

Carina A. Rodriguez ◽

...

Keyword(s):

Tampa Bay ◽

Hiv Status ◽

Health Clinics ◽

Home Testing ◽

Self Testing ◽

Significant Difference ◽

The Us ◽

Study Population ◽

Location Study ◽

Sexual Health Clinics

In adults, data support the utility and acceptance of home HIV testing; however, in youth, particularly in the US, this has not been well studied. In this exploratory study, we surveyed Tampa Bay youth aged 16−27 and attending sexual health clinics between 1 June and 31 June 2018 (n = 133) regarding attitudes and perceptions towards HIV self-testing. While most indicated the clinic over home when asked for preferred testing location, study population and subgroup analysis demonstrated a positive response (agree) to Likert-scale questions regarding the use of home HIV self-testing kits and negative responses (strongly disagree) to “would not use self-testing kit”. There was a significant difference between genders in testing location preference (p = 0.031) for those respondents that specified gender (n = 123), with males more likely to prefer home testing than females. This study suggests an openness of youth towards HIV home testing that could help to expand the number of youth aware of their HIV status.

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Sinonasal effects of external dacryocystorhinostomy

The Journal of Laryngology & Otology ◽

10.1017/s002221512100181x ◽

2021 ◽

pp. 1-4

Author(s):

H Eyigor ◽

E A Cetinkaya ◽

D T Coban ◽

G Ozturk ◽

Ö Erdem

Keyword(s):

Mucociliary Clearance ◽

Nasal Symptom ◽

Ciliary Activity ◽

Test Results ◽

Saccharine Test ◽

Significant Difference ◽

Symptom Scores ◽

Endoscopic Score ◽

External Dacryocystorhinostomy

Abstract Objective External dacryocystorhinostomy is thought to cause mucociliary dysfunction by damaging the mucosa, in turn affecting ciliary activity and mucus quality. This study investigated the effect of external dacryocystorhinostomy on sinonasal function. Methods Patients scheduled for unilateral external dacryocystorhinostomy who underwent endoscopic nasal examination and paranasal sinus computed tomography were included in this study. A saccharine test was performed on the planned surgical side and the mucociliary clearance time was determined. The sinonasal quality of life was measured in all patients, pre-operatively and at six months post-operatively, using the Sino-Nasal Outcome Test-22. The Lund–Kennedy endoscopic score was also determined in all patients, both pre- and post-operatively. Results The study comprised 28 patients (22 females and 6 males). A statistically significant difference was found between the pre- and post-operative saccharine test results (p = 0.006), but not between the pre- and post-operative Sino-Nasal Outcome Test-22 scores (p > 0.05). Conclusion This study is one of only a few to investigate the effect of external dacryocystorhinostomy on sinonasal function. The results showed that external dacryocystorhinostomy impairs mucociliary clearance. The surgical procedure is well tolerated and does not significantly change nasal symptom scores.

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