scholarly journals Insights into the nutritional properties and microbiome diversity in sweet and sour yogurt manufactured in Bangladesh

2021 ◽  
Author(s):  
S. M. Rafiqul Islam ◽  
Afsana Yeasmin Tanzina ◽  
Md Javed Foysal ◽  
M. Nazmul Hoque ◽  
AMAM Z SIDDIKI ◽  
...  
Keyword(s):  
Microbial Diversity ◽  
High Throughput Sequencing ◽  
Starter Culture ◽  
Total Solid ◽  
Nutritional Composition ◽  
Taxonomic Richness ◽  
Nutritional Properties ◽  
Safe Production ◽  
Microbiome Diversity ◽  
Significant Difference

Yogurt quality mainly depends on nutritional properties, microbial diversity and purity of starter culture. This study aimed to assess the nutritional composition and microbiome diversity in yogurt. Microbial diversity was analyzed by 16S and 18S rRNA based high-throughput sequencing. Significantly (P<0.05) higher pH, fat, moisture, total solid and solid-non-fat contents (%) were observed in sweet yogurt whereas sour varieties had significantly higher ash and minerals. Metagenomic investigation showed that 44.86% and 55.14% reads were assigned to bacterial and fungal taxa, respectively, with significantly higher taxonomic richness in sour yogurt. A significant difference in bacterial (Ppermanova=0.001) and fungal (Ppermanova=0.013) diversity between sweet and sour yogurt was recorded. We detected 76 bacterial and 70 fungal genera across these samples which were mostly represented by Firmicutes (>92%) and Ascomycota (98%) phyla, respectively. Among the detected genera, 36.84% bacterial and 22.86% fungal genera were found in both yogurt types. Our results suggest that Streptococcus (50.82%), Lactobacillus (39.92%), Enterobacter (4.85%), Lactococcus (2.84%) and Aeromonas (0.65%) are the most abundant bacterial genera, while Kluyveromyces (65.75%), Trichosporon (8.21%), Clavispora (7.19%), Candida (6.71%), Iodophanus (2.22%), Apiotrichum (1.94%), and Issatchenkia (1.35%) are the most abundant fungal genera in yogurt metagenomes. This is the first study on nutritional properties and microbiome diversity of Bangladeshi yogurt that would be a benchmark for safe production of quality yogurt by commercial manufacturers.

scholarly journals Insights into the nutritional properties and microbiome diversity in sweet and sour yogurt manufactured in Bangladesh

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
S. M. Rafiqul Islam ◽  
Afsana Yeasmin Tanzina ◽  
Md Javed Foysal ◽  
M. Nazmul Hoque ◽  
Meheadi Hasan Rumi ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Its Region ◽  
Total Solid ◽  
Nutritional Composition ◽  
Rrna Gene ◽  
Food Borne ◽  
Nutritional Analysis ◽  
Microbiome Diversity ◽  
Significant Difference ◽  
Nutritional Benefits

AbstractYogurt is one of the most frequently consumed dairy products for nutritional benefits. Although yogurt is enriched with probiotics, it is susceptible to spoilage because of the presence of pathogenic microbes. Spoiled yogurt if consumed can cause food-borne diseases. This study aimed to assess the nutritional composition and microbiome diversity in yogurt manufactured in Bangladesh. Microbial diversity was analyzed through high-throughput sequencing of bacterial 16S rRNA gene and fungal internal transcribed spacer (ITS) region. From nutritional analysis, significantly (P < 0.05) higher pH, fat, moisture, total solid and solid-non-fat contents (%) were observed in sweet yogurt. Following the classification of Illumina sequences, 84.86% and 72.14% of reads were assigned to bacterial and fungal genera, respectively, with significantly higher taxonomic richness in sour yogurt prepared from buffalo. A significant difference in bacterial (Ppermanova = 0.001) and fungal (Ppermanova = 0.013) diversity between sweet and sour yogurt was recorded. A total of 76 bacterial and 70 fungal genera were detected across these samples which were mostly represented by Firmicutes (92.89%) and Ascomycota (98%) phyla, respectively. This is the first study that accentuates nutritional profiles and microbiome diversity of Bangladeshi yogurt which are crucial in determining both active and passive health effects of yogurt consumption in individuals.

scholarly journals 1381. Do Gut Microbiome Profiles Correlate with Hospital Length of Stay During Hematopoietic Stem Cell Transplantation?

2021 ◽  
Vol 8 (Supplement_1) ◽  
pp. S776-S777
Author(s):  
Angelico Mendy ◽  
Kitty Tierney ◽  
Tara Mink ◽  
Walaa Hussein ◽  
Peter Monaco ◽  
...  
Keyword(s):  
Stem Cell ◽  
Length Of Stay ◽  
Microbial Diversity ◽  
Hematopoietic Stem Cell ◽  
Gut Microbiome ◽  
P Value ◽  
Inpatient Setting ◽  
Hematopoietic Stem ◽  
Microbiome Diversity ◽  
Significant Difference

Abstract Background Length of stay is not only an indicator of how successful a hospitalized patient’s treatment and recovery is, but is also an indicator of fiscal costs to the hospital. Hematopoietic stem cell transplants (HSCT) patients typically experience extended hospital admissions that can vary significantly patient to patient with hospital discharge dependent upon a recovered white blood cell count. Recent literature suggests a gut microbial influence on hematopoiesis. We sought to explore potential associations between gut microbiome diversity and the length of stay in patients undergoing HSCT in the inpatient setting. Methods Within two healthcare systems, we identified patients who would receive conditioning chemotherapy and subsequent HSCT in the inpatient setting. Pre-chemotherapy stool was collected, sequenced with shotgun metagenomics, and analyzed for gut microbial diversity using Inverse-Simpson index. The length of admission or length of stay during their transplant process was recorded. We assessed whether there was an association with gut microbial diversity and length of stay. Results 24 patients we evaluated for diversity and length of stay. There was no significant correlation between age or gender and length of stay. Significant difference in length of stay was seen between allogenic vs autogenic transplants (p value ≤0.01). Within the 24 patients, lengths of stay ranged from 8 to 36 days with a mean average of 20.9 days. Gut diversity ranged from 1.8 to 23.9. An overall negative association between length of stay and diversity was seen, though this was determined not statistically significant (p value 0.09). Length of Stay correlation with pre-chemotherapy Gut Microbiome diversity Conclusion Our study showed no significant association between gut microbial diversity and inpatient length of stay during HSCT. Overall, a trend towards increased length of stay in patients with decreased diversity was noted. Additional studies of greater participant size are necessary to confirm or further study these findings. Disclosures All Authors: No reported disclosures

scholarly journals EFFECT OF DIFFERENT PROCESSING METHODS ON THE NUTRITIONAL COMPOSITION AND ANTINUTRITIONAL FACTORS OF PEANUT MILK

2021 ◽  
Vol 108 (March) ◽  
pp. 1-5
Author(s):  
Siva sakthi T ◽  
◽  
Meenakshi V ◽  
Kanchana S ◽  
Vellaikumar S ◽  
...  
Keyword(s):  
Trypsin Inhibitor ◽  
Iron Content ◽  
Total Solid ◽  
Antinutritional Factors ◽  
Nutritional Composition ◽  
Solid Content ◽  
Antinutritional Factor ◽  
Nutritional Properties ◽  
Total Solid Content ◽  
Processing Methods

Comparative effects of different processing methods like blanching, soaking, roasting and germination methods on nutritional composition and antinutritional factors in peanut milk were investigated. Local and CO 6 peanut varieties were selected for peanut milk preparation and subjected to different processing methods. Peanut milk was extracted from fresh (control), blanched (2 mins), soaked (3 hrs), roasted (roasting 5 mins followed by soaking 3 hrs) and germinated (8 hrs) peanuts. Peanuts were washed, ground, slurry separated, filtered, homogenized, double pasteurized and stored. Different processing methods influenced the nutritional composition of local and CO 6 variety peanut milk. The nutritional composition like total solids, protein, fat, calcium and iron content of the different processed peanut milk were analyzed. The total solid content of the peanut milk does not change in soaking (13.98 and 15 g/ 100 mL), germination method (13.99 and 15.26 g/100 mL), less reduction in roasting method (13.96 and 15.20 g/ 100 mL), more significant reduction in blanching method (12.36 and 13.97 g/ 100 mL) in local and CO 6 variety of peanut milk respectively were recorded. Protein content was increased in germination ((6.1 and 6.4 g/ 100 mL), soaking (6.0 and 6.3 g / 100 mL), roasting method (5.6 and 5.8 g / 100 mL) and reduction in blanching method (4.1 and 5.0 g / 100 mL) in local and CO 6 variety peanut milk respectively. Fat content was significantly reduced in control (3.82 and 3.54 g / 100 mL), blanching method (3.63 and 3.32 g / 100 mL), soaking method ( 3.80 and 3.55 g/ 100 mL) and germination method (3.60 and 3.50 g/ 100 mL) in local and CO 6 variety peanut milk. Calcium and iron content was greatly reduced in blanching and lesser reduction in roasting method. Antinutritional factors like phytic acid, tannin, trypsin inhibitor, oxalate and phenol was greatly reduced in roasting and germination methods. It was observed that the nutritional properties were highly conserved and the antinutritional factor was greatly reduced in the roasting method.

scholarly journals An Active Gelatin Coating Containing Eugenol and Vacuum Delays the Decay of Chinese Seabass (Lateolabrax maculatus) Fillets during Cold Storage: A Microbiome Perspective

Coatings ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 147
Author(s):  
Xuan Ma ◽  
Qianqian Zhou ◽  
Weiqiang Qiu ◽  
Jun Mei ◽  
Jing Xie
Keyword(s):  
Microbial Diversity ◽  
High Throughput Sequencing ◽  
Vacuum Treatment ◽  
C Storage ◽  
Basic Nitrogen ◽  
Spoilage Bacteria ◽  
Lateolabrax Maculatus ◽  
Gelatin Coating ◽  
Microbial Enumeration ◽  
Growth And Reproduction

The purpose of this study was to evaluate the effect of an active gelatin coating containing eugenol and vacuum on the microbial diversity of Chinese seabass (Lateolabrax maculatus) during cold (−0.9 °C) storage. The bacterial sequences in Chinese seabass were observed using a high-throughput sequencing technique targeting the V3–V4 region of the 16S Ribosomal DNA (rDNA) on 0, 12th, and 24th day, which showed a more comprehensive estimate of the microbial diversity in seabass samples compared with microbial enumeration. The results revealed that the species diversity of fresh seabass was rich, mainly including Carnobacterium, Glutamicibacter, and Pseudomonas, with abundance ratios of 0.286, 0.160, and 0.130, respectively. Pseudomonas and Shewanella were the primary contaminants in the spoiled control samples, where the abundance ratios increased from 0.220 and 0.174 on the 12th day to 0.802 and 0.163 on the 24th day, respectively. Vacuum treatment could inhibit the growth of Pseudomonas and Shewanella such that when stored on the 12th day, Brochothrix became the superior genus. However, Pseudomonas and Shewanella dominated the storage until the 24th day, where their abundance ratios were 0.343 and 0.279, respectively. The inhibition of Pseudomonas and Carnobacterium was gradually enhanced with increasing concentrations of eugenol. Furthermore, an active gelatin coating containing eugenol and vacuum treatment was more effective at inhibiting the increase of the total volatile basic nitrogen. This study confirmed that an active gelatin coating containing eugenol and vacuum could reduce the species of bacteria, inhibit the growth and reproduction of the main dominant spoilage bacteria, and delay the spoilage of seabass.

scholarly journals Nutritional and Functional Evaluation of Inula crithmoides and Mesembryanthemum nodiflorum Grown in Different Salinities for Human Consumption

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4543
Author(s):  
Alexandre Lima ◽  
Florinda Gama ◽  
Viana Castañeda-Loaiza ◽  
Camila Costa ◽  
Lisa M. Schüler ◽  
...  
Keyword(s):  
Salt Stress ◽  
Vitamin B1 ◽  
Nutritional Composition ◽  
Human Consumption ◽  
Functional Evaluation ◽  
Cultivation System ◽  
Safe Production ◽  
Soilless Cultivation ◽  
Relative Productivity ◽  
Β Carotene

The nutritional composition and productivity of halophytes is strongly related to the biotic/abiotic stress to which these extremophile salt tolerant plants are subjected during their cultivation cycle. In this study, two commercial halophyte species (Inula crithmoides and Mesembryanthemum nodiflorum) were cultivated at six levels of salinity using a soilless cultivation system. In this way, it was possible to understand the response mechanisms of these halophytes to salt stress. The relative productivity decreased from the salinities of 110 and 200 mmol L−1 upwards for I. crithmoides and M. nodiflorum, respectively. Nonetheless, the nutritional profile for human consumption remained balanced. In general, I. crithmoides vitamin (B1 and B6) contents were significantly higher than those of M. nodiflorum. For both species, β-carotene and lutein were induced by salinity, possibly as a response to oxidative stress. Phenolic compounds were more abundant in plants cultivated at lower salinities, while the antioxidant activity increased as a response to salt stress. Sensory characteristics were evaluated by a panel of culinary chefs showing a preference for plants grown at the salt concentration of 350 mmol L−1. In summary, salinity stress was effective in boosting important nutritional components in these species, and the soilless system promotes the sustainable and safe production of halophyte plants for human consumption.

scholarly journals Environmental Sequencing Provides Reasonable Estimates of the Relative Abundance of Specific Picoeukaryotes

2016 ◽  
Vol 82 (15) ◽  
pp. 4757-4766 ◽  
Author(s):  
Caterina R. Giner ◽  
Irene Forn ◽  
Sarah Romac ◽  
Ramiro Logares ◽  
Colomban de Vargas ◽  
...  
Keyword(s):  
Microbial Diversity ◽  
High Throughput ◽  
Relative Abundance ◽  
Ribosomal Dna ◽  
18S Rdna ◽  
High Throughput Sequencing ◽  
454 Sequencing ◽  
Epifluorescence Microscopy ◽  
Environmental Sequencing

ABSTRACTHigh-throughput sequencing (HTS) is revolutionizing environmental surveys of microbial diversity in the three domains of life by providing detailed information on which taxa are present in microbial assemblages. However, it is still unclear how the relative abundance of specific taxa gathered by HTS correlates with cell abundances. Here, we quantified the relative cell abundance of 6 picoeukaryotic taxa in 13 planktonic samples from 6 European coastal sites using epifluorescence microscopy on tyramide signal amplification-fluorescencein situhybridization preparations. These relative abundance values were then compared with HTS data obtained in three separate molecular surveys: 454 sequencing of the V4 region of the 18S ribosomal DNA (rDNA) using DNA and RNA extracts (DNA-V4 and cDNA-V4) and Illumina sequencing of the V9 region (cDNA-V9). The microscopic and molecular signals were generally correlated, indicating that a relative increase in specific 18S rDNA was the result of a large proportion of cells in the given taxa. Despite these positive correlations, the slopes often deviated from 1, precluding a direct translation of sequences to cells. Our data highlighted clear differences depending on the nucleic acid template or the 18S rDNA region targeted. Thus, the molecular signal obtained using cDNA templates was always closer to relative cell abundances, while the V4 and V9 regions gave better results depending on the taxa. Our data support the quantitative use of HTS data but warn about considering it as a direct proxy of cell abundances.IMPORTANCEDirect studies on marine picoeukaryotes by epifluorescence microscopy are problematic due to the lack of morphological features and due to the limited number and poor resolution of specific phylogenetic probes used in fluorescencein situhybridization (FISH) routines. As a consequence, there is an increasing use of molecular methods, including high-throughput sequencing (HTS), to study marine microbial diversity. HTS can provide a detailed picture of the taxa present in a community and can reveal diversity not evident using other methods, but it is still unclear what the meaning of the sequence abundance in a given taxon is. Our aim is to investigate the correspondence between the relative HTS signal and relative cell abundances in selected picoeukaryotic taxa. Environmental sequencing provides reasonable estimates of the relative abundance of specific taxa. Better results are obtained when using RNA extracts as the templates, while the region of 18S ribosomal DNA had different influences depending on the taxa assayed.

COMPARATIVE EVALUATION OF YOGHURT SAMPLES FROM GOATS AND COWS MILK AND COMMERCIAL RETAIL OUTLET

2021 ◽  
Vol 34 (1) ◽  
Author(s):  
V. A. Obatolu ◽  
E. A. Adebowale ◽  
F. Omidokun ◽  
E. O. Farinde
Keyword(s):  
Goat Milk ◽  
Storage Period ◽  
Total Solid ◽  
Cow Milk ◽  
Microbial Count ◽  
Significant Drop ◽  
Overall Acceptability ◽  
Count Range ◽  
Significant Difference ◽  
Mineral Acidity

This paper compared the chemical (Proximate and mineral), acidity, microbial count and organoleptic properties of yoghurt samples produced from Nigeria locally bred goat and cow milk with commercially retail yoghurt during 14days of refrigerated storage. Yoghurt production was conducted by inoculation of the milk samples at 43oC for 6hrs with 2% of streptococcus thermophillus and lactobacillus bulgarus active culture. The total ash content of yoghurt ranges from 0.23g/100g in the commercial yoghurt sample to 0.84g/100g in yoghurt from goat  milk which is considered significantly higher than that observed for yoghurt from cow milk. The commercially retail yoghurt had a significantly higher (P < 0.05) total solid matter than yoghurt from goat and cow milk. Calcium and phosphorus contents were significantly (P < 0.05) lowest in the commercial yoghurt (0.28%) to a significant higher value of 0.28% in goat milk. A statisitically significant drop in pH values was observed in the commercial yoghurt sample after one and two week storage compared to yoghurt from goat and cow milk. High lactic acid bacteria were observed in yoghurt from cow milk with lactococci group being higher than the lactobacilli group throughout the storage period. The yeast count range from a significant low (P < 05) value of 2.22 cfu/ml-1 in commercial yoghurt sample to 3.22 and 3.24 in goat and cow milk respectively on day zero of storage. By the 7days of storage, the commercially retail yoghurt was significant (P < 0.05) least desirable with respect to colour, taste and overall acceptability while there was no significant difference in the consistency of all the yoghurt samples.

Periphyton diversity in two different Antarctic lakes assessed using metabarcoding

2021 ◽  
pp. 1-9
Author(s):  
Paulo E.A.S. Câmara ◽  
Láuren M.D. De Souza ◽  
Otávio Henrique Bezerra Pinto ◽  
Peter Convey ◽  
Eduardo T. Amorim ◽  
...  
Keyword(s):  
Dna Sequences ◽  
High Throughput Sequencing ◽  
Shannon Index ◽  
South Shetland Islands ◽  
King George Island ◽  
Deception Island ◽  
Maritime Antarctic ◽  
Shetland Islands ◽  
Antarctic Lakes ◽  
Significant Difference

Abstract Antarctic lakes have generally simple periphyton communities when compared with those of lower latitudes. To date, assessment of microbial diversity in Antarctica has relied heavily on traditional direct observation and cultivation methods. In this study, sterilized cotton baits were left submerged for two years in two lakes on King George Island and Deception Island, South Shetland Islands (Maritime Antarctic), followed by assessment of diversity by metabarcoding using high-throughput sequencing. DNA sequences of 44 taxa belonging to four kingdoms and seven phyla were found. Thirty-six taxa were detected in Hennequin Lake on King George Island and 20 taxa were detected in Soto Lake on Deception Island. However, no significant difference in species composition was detected between the two assemblages (Shannon index). Our data suggest that metabarcoding provides a suitable method for the assessment of periphyton biodiversity in oligotrophic Antarctic lakes.

scholarly journals Comparison of Oral Hygiene Status and Relative Abundance of Periodontal Pathogens Between Individuals With and Without Intellectual Disabilities: a Cross-Sectional Study

2020 ◽  
Author(s):  
Yuki Oda ◽  
Chiaki Furutani ◽  
Reo Kawano ◽  
Jumpei Murakami ◽  
Yuika Mizota ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Relative Abundance ◽  
Outpatient Clinic ◽  
High Throughput Sequencing ◽  
Analysis Of Covariance ◽  
University Hospital ◽  
Permanent Teeth ◽  
Control Group ◽  
Periodontal Pathogens ◽  
Significant Difference

Abstract Background: Studies have reported a high prevalence of periodontal disease in individuals with intellectual disability (ID). The aim of this study was to compare the relative abundance of periodontal pathogens (red, orange, yellow, purple, and green complexes) between individuals with ID and healthy controls.Methods: Of the 31 subjects enrolled in this study, 16 with severe ID were selected from the outpatient clinic of the Special Care Dentistry of Hiroshima University Hospital, and 14 healthy subjects were selected from the outpatient clinic of another department at the same hospital. Dental plaque was sampled after oral examination. Decayed, missing, and filled permanent teeth (DMFT) counts were obtained and periodontal measurements were taken using the papillary-marginal-attached (PMA) index, plaque index (PI), gingival index (GI), and probing depth (PD). Gene sequencing (16S rRNA) was performed for each sample using next-generation high-throughput sequencing methods. The relative abundance of the periodontal pathogens and the clinical parameters were compared. An unpaired t-test was used to compare the oral health status in both groups and analysis of covariance was performed to compare the relative abundance of each pathogen.Results: No statistically significant difference in DMFT was observed between the two groups. However, significant differences in the median PMA index, PI, and GI were noted between the groups (P < 0.0001). In addition, the mean PD in the ID group was significantly higher than that in the control group (P < 0.0001). The relative abundances of Tannerella spp. and Treponema spp. were significantly higher in the ID group when compared to the control group at the genus level (P = 0.0383 and 0.0432, respectively); alternatively, the relative abundance of Porphyromonas spp. was significantly lower in the ID group (P < 0.0001).Conclusions: The PMA index, PI, GI, and PD were significantly lower in the ID group than in the control group. On the other hand, no significant difference in DMFT was observed between the two groups. Furthermore, our findings indicate that Tannerella forsythia might be more closely associated with periodontal disease than Porphyromonas gingivalis in individuals with ID.

scholarly journals Glucocorticoids coordinate changes in gut microbiome composition in wild North American red squirrels

2021 ◽  
Author(s):  
Lauren Petrullo ◽  
Tiantian Ren ◽  
Martin Wu ◽  
Rudy Boonstra ◽  
Rupert Palme ◽  
...  
Keyword(s):  
Microbial Diversity ◽  
North American ◽  
Gut Microbiome ◽  
Pathogenic Bacteria ◽  
Experimental Studies ◽  
Ecological Framework ◽  
Ecological Change ◽  
Red Squirrels ◽  
Microbiome Diversity ◽  
North American Red Squirrels

Gut microbiome diversity plays an important role in host health and fitness, in part through the diversification of gut metabolic function and pathogen protection. Elevations in glucocorticoids (GCs) appear to reduce gut microbiome diversity in experimental studies, suggesting that a loss of microbial diversity may be a negative consequence of increased GCs. However, given that ecological factors like food availability and population density may independently influence both GCs and microbial diversity, understanding how these factors structure the GC-microbiome relationship is crucial to interpreting its significance in wild populations. Here, we used an ecological framework to investigate the relationship between GCs and gut microbiome diversity in wild North American red squirrels (Tamiasciurus hudsonicus). We found that higher GCs predicted lower gut microbiome diversity and an increase in metabolic taxa. In addition, we identified a loss of potentially pathogenic bacteria with increasing GCs. Both dietary heterogeneity and an upcoming masting event exhibited direct effects on gut microbiome diversity, whereas conspecific density and host reproductive activity impacted diversity indirectly via changes in GCs. Together, our results suggest that GCs coordinate the effects of ecological change and host biology on gut microbiome diversity, and highlight the importance of situating the GC-microbiome relationship within an ecological framework.

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