scholarly journals ITRAQ-based quantitative proteomic analysis of japonica rice seedling during cold stress

2021 ◽  
Author(s):  
Dongjin Qing ◽  
Yinghua Pan ◽  
Gaoxing Dai ◽  
Lijun Gao ◽  
Haifu Liang ◽  
...  
Keyword(s):  
Cold Stress ◽  
Stress Responses ◽  
Protein Level ◽  
Quantitative Proteomics ◽  
Rice Variety ◽  
Mrna Level ◽  
Growth And Yield ◽  
Pcr Analysis ◽  
Control Group ◽  
Japonica Rice

Low temperature is one of the important environmental factors that affect rice growth and yield. To better understand the japonica rice responses to cold stress, isobaric tags for relative and absolute quantification (iTRAQ) labeling based quantitative proteomics approach was used to detected changes in protein level. Two-week-old seedlings of the cold tolerance rice variety Kongyu131 were treated at 8? for 24, 48 and 72 h, then the total proteins were extracted from tissues and used for quantitative proteomics analysis. A total of 5082 proteins were detected for quantitative analysis, of which 289 proteins were significantly regulated, consisting of 169 uniquely up-regulated proteins and 125 uniquely down-regulated proteins in cold stress groups relative to control group. Functional analysis revealed most of regulation proteins involved in photosynthesis, metabolic pathway, biosynthesis of secondary metabolites and carbon metabolism. Western blot analysis showed that protein regulation was consistent with the iTRAQ data. The corresponding genes of 25 regulation proteins were used for quantitative real time PCR analysis, and the results showed that the mRNA level was not always parallel to the corresponding protein level. The importance of our study is providing new insights into cold stress responses in rice on proteomic aspect.

scholarly journals Effects of Acupuncture, RU-486 on the Hypothalamic-Pituitary-Adrenal Axis in Chronically Stressed Adult Male Rats

Endocrinology ◽  
2015 ◽  
Vol 156 (10) ◽  
pp. 3649-3660 ◽  
Author(s):  
Ladan Eshkevari ◽  
Susan E. Mulroney ◽  
Rupert Egan ◽  
Lixing Lao
Keyword(s):  
Cold Stress ◽  
Adult Male ◽  
Stress Responses ◽  
Male Rats ◽  
Control Group ◽  
Ru 486 ◽  
Adrenal Axis ◽  
Stomach Meridian ◽  
Anxious Behavior ◽  
Pituitary Adrenal Axis

We have recently reported that pretreatment with electroacupuncture (EA) at stomach meridian point 36 (St36) prevents the chronic cold-stress increase in the hypothalamus-pituitary-adrenal axis (HPA), an action that may be under central control. Given that treatment for stress-related symptoms usually begins after onset of the stress responses, the objectives of the present study were to determine the efficacy of EA St36 on HPA hormones when EA St36 is given after stress was initiated, if the results are long lasting, and if blocking the glucocorticoid receptor (GR) using RU-486 had the same effects as EA St36. Adult male rats were placed in 4 groups of animals, 3 of which were exposed to cold and 1 of which was a nontreatment control group. After exposure to the cold stress, 2 groups were treated with either EA St36 or sham-EA, repeated over 10 days. The increase in ACTH and corticosterone observed in stress-only rats was prevented in EA St36 animals, and the effects remained intact 4 days after withdrawal of EA but continuation of cold stress. When the GR was blocked with RU-486, the efficacy of EA St36 remained unchanged. GR blockade did significantly elevate ACTH, which is not seen with EA St36, suggesting that EA St36 does act centrally. The elevated HPA hormones in stress-only rats were associated with a significant increase in depressive and anxious behavior; this was not observed in the stressed EA St36 animals. The results indicate that EA specifically at St36 vs sham-EA is effective in treating chronic poststress exposure.

The Wheat Aquaporin Gene TaAQP7 Confers Tolerance to Cold Stress in Transgenic Tobacco

2014 ◽  
Vol 69 (3-4) ◽  
pp. 142-148 ◽  
Author(s):  
Chao Huang ◽  
Shiyi Zhou ◽  
Wei Hu ◽  
Xiaomin Deng ◽  
Shuya Wei ◽  
...  
Keyword(s):  
Cold Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Stress Responses ◽  
Cold Treatment ◽  
Membrane Damage ◽  
Pcr Analysis ◽  
Ion Leakage ◽  
Chlorophyll Contents ◽  
Aquaporin Gene

Aquaporin proteins (AQPs) have been shown to be involved in abiotic stress responses. However, the precise role of AQPs, especially in response to cold stress, is not understood in wheat (Triticum aestivum). In the present study, quantitative real time polymerase chain reaction (qRT-PCR) analysis revealed that TaAQP7 expression increased in leaves, but decreased in roots after cold treatment. Expression of TaAQP7 in tobacco plants resulted in increased root elongation and better growth compared with wild-type (WT) plants under cold stress. Moreover, after cold treatment, the transgenic tobacco lines exhibited higher chlorophyll contents, lower levels of malondialdehyde (MDA), and less ion leakage (IL) than WT plants. Thus, expression of TaAQP7 enhanced cold stress tolerance in transgenic tobacco. Taken together, our results suggest that TaAQP7 confers cold stress tolerance by relieving membrane damage in the transgenic plants.

scholarly journals The effects of the metformin on inhibition of UVA-induced expression of MMPs and COL-I in human skin fibroblasts

2019 ◽  
Vol 17 ◽  
pp. 205873921987642
Author(s):  
Bangsheng Cui ◽  
Qi Liu ◽  
Ling Tong ◽  
Xuefeng Feng
Keyword(s):  
Human Skin ◽  
Protein Level ◽  
Messenger Rna ◽  
Mrna Level ◽  
Skin Fibroblasts ◽  
Control Group ◽  
Type I ◽  
Mrna Levels ◽  
Human Skin Fibroblasts ◽  
Induced Expression

This study was to investigate the effects of metformin (MF) on ultraviolet A (UVA)-induced expression of matrix metalloproteinases (MMPs) and type I collagen (COL-I) in human skin fibroblasts (HSFs). HSFs were cultured in vitro and divided into control group, UVA group, and UVA + MF group. Cell proliferation was detected by CCK-8 method, and intracellular reactive oxygen species (ROS) level was detected by flow cytometry with fluorescent probe 2′,7′-dichlorofluorescein diacetate (DCF-DA) staining. Meanwhile, real-time polymerase chain reaction (PCR) was used to examine the relative messenger RNA (mRNA) expression of aging-related genes, including MMP1, MMP3, and COL-I. Moreover, the expression of MMP1, MMP3, and COL-I proteins was further detected by western blot. Compared with the control group, the ROS content in the UVA group was increased significantly ( P < 0.05), while the ROS content in the UVA + MF group was evidently lower than that in the UVA group ( P < 0.05). In addition, the MMP1 and MMP3 mRNA levels were significantly elevated, while the COL-I mRNA was significantly decreased in UVA-induced HSF cells compared with the control cells. However, MF could significantly inhibit the improved MMP1 and MMP3 mRNA level and increase the COL-I mRNA level. Moreover, MF could significantly reverse the increasing MMP1 and MMP3 protein level and decreasing COL-I protein level induced by UVA. In conclusion, MF can increase the antioxidant capacity of cells and increase the synthesis of COL-I by inhibiting the level of intracellular ROS and the expression of related MMPs, thereby inhibiting the UVA-induced photoaging effect of HSF.

Development of transgenic Brassica napus with an optimized cry1C* gene for resistance to diamondback moth (Plutella xylostella)

2014 ◽  
Vol 94 (8) ◽  
pp. 1501-1506 ◽  
Author(s):  
Yaqin Wang ◽  
Yan Zhang ◽  
Fang Wang ◽  
Chao Liu ◽  
Kede Liu
Keyword(s):  
Brassica Napus ◽  
Plutella Xylostella ◽  
Protein Level ◽  
Diamondback Moth ◽  
Mrna Level ◽  
Pcr Analysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Foreign Gene ◽  
Transgenic Lines ◽  
Transgenic Rapeseed

Wang, Y., Zhang, Y., Wang, F., Liu, C. and Liu, K. 2014. Development of transgenic Brassica napus with an optimized cry1C* gene for resistance to diamondback moth (Plutella xylostella). Can. J. Plant Sci. 94: 1501–1506. Bacillus thuringiensis (Bt) cry1Ac gene has been transformed into rapeseed to control diamondback moth (DBM, Plutella xylostella), which is one of the major lepidopteran pests of rapeseed (Brassica napus). However, Cry1A-resistant DBM populations have already developed in the field. Cry1C* is a new synthetic Bt gene based on the original cry1Ca5 sequence through optimizing its codons as well as removing AT-rich sequences and inverted repeats. In our present study, the cry1C* gene was introduced into rapeseed via Agrobacterium-mediated transformation, and a total of 42 transgenic lines were recovered. The results of polymerase chain reaction (PCR) and Southern blot both confirmed the expression of the cry1C* gene in the genomes of the transformants. We also assessed the expression of this foreign gene at the mRNA level in some selected transgenic lines by real-time reverse transcription (RT) PCR analysis. Enzyme-linked immunosorbent assay (ELISA) showed that the Cry1C* expression at the protein level greatly varied among individual transgenic plants, and transgenic line 1C-8 had the highest protein level of 799.32 ng g−1. The transgenic rapeseed plants expressing cry1C* gene showed a high efficacy against DBM. Taken together, the cry1C*-transgenic rapeseed could be employed as a useful germplasm in pest management and in the broad bioinsecticidal spectrum to prevent and postpone the development of pest resistance.

The Respective Potential of Endurance Training on COUP-TF I and COUP-TF II Gene Expression in the Skeletal Muscle of Rats

2016 ◽  
Vol 13 (10) ◽  
pp. 6993-6998
Author(s):  
Haiyan Wang ◽  
Shuzhe Ding
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Blood Glucose ◽  
Endurance Training ◽  
Protein Level ◽  
Mrna Level ◽  
Control Group ◽  
Apparent Effect ◽  
Gk Rats ◽  
Sd Rats

Objective: The aim of this study was to investigate the effects of 6-week endurance training on COUP-TF I and COUP-TF II gene expression of SD rats and GK rats. Method: 20 male SD rats, about 4 weeks old, weighing 100 ± 5 g; 12 GK rats, about 8 weeks old, weighing 250 ± 5 g, SD rats were randomly divided into SD control group (C, n = 10), endurance group (E, n = 10), GK rats were randomly divided into GK control group (H, n = 6), endurance group (R, n = 6). Endurance training in rats for 6 weeks of treadmill training, training six days a week, once a day for a maximum speed of not more than 16.7 m/min, 1 hour a day. Real-time PCR was used to detect the mRNA level of COUP-TF I and COUP-TF II. Western Blotting was used to detect the protein level COUP-TF I. Results: (1) SD rats: endurance training significantly reduced blood glucose but had no apparent effect on IRI; endurance training significantly increased COUP-TF I mRNA level but significantly reduced COUP-TF II mRNA level; endurance exercises had no apparent effect on COUP-TF I protein level (2) GK rats: endurance training significantly reduced blood glucose and IRI; endurance training both significantly reduced the mRNA level of COUP-TF I and COUP-TF II; endurance training significantly reduced COUP-TF I protein level. Conclusion: (1) Endurance training was beneficial to reduce the level of blood glucose in GK rats, which may mitigate IR. (2) The effects of endurance exercise on COUP-TF I gene expression in skeletal muscle are opposite under normal physiological and diabetes, which may be related to the differences of strains, age and diet between GK rats and SD rats. (3) Compared with COUP-TF I, the adaptation of COUP-TF II mRNA level to endurance exercise was exactly the same for GK rats and SD rats, which indicating that the COUP-TF II mRNA level may play an important role in the effect of endurance training that can prevention and improvement of insulin resistance.

scholarly journals Losartan Protects the Heart Against Ischemia Reperfusion Injury: Sirtuin3 Involvement

2015 ◽  
Vol 18 (1) ◽  
pp. 112 ◽  
Author(s):  
Mohsen Sharifi Klishadi ◽  
Farideh Zarei ◽  
Seyyed Hassan Hejazian ◽  
Ali Moradi ◽  
Mahdieh Hemati ◽  
...  
Keyword(s):  
Protein Level ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Mrna Level ◽  
Experimental Studies ◽  
Control Group ◽  
Signaling Mechanism ◽  
Survival Factors ◽  
Protein Levels ◽  
Ischemic Tissue

PURPOSE: Sirtuin-3 (SIRT3) deacetylase protects the heart against oxidative stress via survival factors upregulation. Clinical and experimental studies have demonstrated that activation of systemic and local renin-angiotensin system (RAS) is implicated in ischemia-induced cardiac injury. However, the relation between RAS and SIRT3 in pathophysiology of myocardial ischemia reperfusion is unknown. In this study, the cardiac transcription and expression of SIRT3 levels was examined in response to ischemia reperfusion in untreated and losartan treated rats. METHODS: Rats were divided into control group, losartan group (L), and ischemia reperfusion (IR) groups with (L+IR) or without losatran pretreatment. Some rats were included as sham-operated and saline groups. IR was induced by left anterior descending artery occlusion. SIRT3 protein levels were determined by Western blot technique. The genes expression was specified by real-time RT-PCR. Arrhythmias were assessed according to the Lambeth conventions. RESULTS: In L+IR group a significant reduction was noted in the number of ventricular ectopic beats (VEBs) and episodes of ventricular tachycardia (VT) (VEBs: P<0.001; VT: P<0.01 vs. IR). In IR group, SIRT3 protein level was decreased in the ischemic tissue by 26.7±5.9% (P<0.01 vs. Control). However, in the non-ischemic tissue the changes of SIRT3 protein content were not significant. In L+IR group SIRT3 protein levels in the ischemic part of Left ventricle were significantly different from IR group (P<0.001). SIRT3 mRNA level did not change significantly among the experimental groups. Thioredoxin-1 and catalase transcription level was increased in L+IR group compared to IR group (P<0.01). CONCLUSION: A decreased SIRT3 protein levels subsequent to IR might be a novel signaling mechanism involved in IR injury. Losartan at non–hypotensive dose exerts anti-ischemic effects in part by normalizing the SIRT3 protein level and upregulating the survival factors encoding genes transcription in ischemic tissue of the heart. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

scholarly journals CpG Methylation of the GPX3 Promoter in Patients with Kashin-Beck Disease Potentially Promotes Chondrocyte Apoptosis

2021 ◽  
Author(s):  
Rongqiang Zhang ◽  
Di Zhang ◽  
Xiaoli Yang ◽  
Dandan Zhang ◽  
Qiang Li ◽  
...  
Keyword(s):  
Promoter Region ◽  
Healthy Subjects ◽  
Mrna Level ◽  
Chondrocyte Apoptosis ◽  
Pcr Analysis ◽  
Control Group ◽  
Mrna Levels ◽  
Injury Group ◽  
Blood Specimens ◽  
Methylation Patterns

Abstract Objective: To determine the methylation levels of CpGs in the GPX3 promoter region and explore their potential effects on the apoptosis of chondrocytes.Methods: Blood specimens were collected from 32 participants, including 16 KBD patients and 16 healthy subjects. Twenty-five CpGs in the promoter region of GPX3 were identified and detected by MALDI-TOF-MS. Methylation levels of CpGs were compared between KBD patients and healthy subjects as well as among the KBD patients with different degrees. C28/I2 human chondrocytes were treated with tBHP and Na2SeO3. Apoptosis in chondrocytes was examined under a fluorescence microscope.Results: The methylation levels of GPX3-8_CpG_11, GPX3-8_CpG_16, GPX3-8_CpG_20 in KBD patients were significantly higher than those of healthy subjects (P <0.05). The methylation levels of the other CpGs were not significantly different between the two groups (P> 0.05). The methylation level of GPX3-8_CpG_24 in KBD patients with degree III was significantly higher than those in KBD patients with degree I/II (P <0.05) MSP-PCR analysis indicated that the methylation rate of KBD group (9.41%) was significantly higher than that of healthy subjects (1.18%), and that GPX3 DNA methylation increased the risk of acquiring KBD 8 fold (OR = 8.000, 95% CI : 1.023-62.580); The mRNA expression of GPX3 in whole blood of KBD patients was lower than that of healthy subjects (P＜0.05); Compared with the control group, GPX3, GPX1 and GPX4 mRNA level of the tertbutyl hydroperoxide injury group decreased significantly (P <0.05), after supplementation with Na2SeO3. The logarithmic increase in mRNA levels of GPX3, GPX1 and GPX4 decreased the rate of apoptosis in chondrocytes.Conclusion: The methylation patterns of CpGs in GPX3 varied in KBD patients. The experiments indicated that the increased methylation of CpGs within the GPX3 promoter may down-regulate the expression of GPX3, thereby reducing the antioxidant function of GPX3 and promoting chondrocyte apoptosis, both of which accelerates the development of KBD.

scholarly journals Combining QTL-seq and linkage mapping to fine map a candidate gene in qCTS6 for cold tolerance at the seedling stage in rice

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Luomiao Yang ◽  
Jingguo Wang ◽  
Zhenghong Han ◽  
Lei Lei ◽  
Hua Long Liu ◽  
...  
Keyword(s):  
Cold Stress ◽  
Cold Tolerance ◽  
Regulatory Gene ◽  
Snp Markers ◽  
Seedling Stage ◽  
Pcr Analysis ◽  
Sequencing Data ◽  
Coding Region ◽  
Rice Varieties ◽  
Major Qtl

Abstract Background Cold stress caused by low temperatures is an important factor restricting rice production. Identification of cold-tolerance genes that can stably express in cold environments is crucial for molecular rice breeding. Results In this study, we employed high-throughput quantitative trait locus sequencing (QTL-seq) analyses in a 460-individual F2:3 mapping population to identify major QTL genomic regions governing cold tolerance at the seedling stage in rice. A novel major QTL (qCTS6) controlling the survival rate (SR) under low-temperature conditions of 9°C/10 days was mapped on the 2.60-Mb interval on chromosome 6. Twenty-seven single-nucleotide polymorphism (SNP) markers were designed for the qCST6 region based on re-sequencing data, and local QTL mapping was conducted using traditional linkage analysis. Eventually, we mapped qCTS6 to a 96.6-kb region containing 13 annotated genes, of which seven predicted genes contained 13 non-synonymous SNP loci. Quantitative reverse transcription PCR analysis revealed that only Os06g0719500, an OsbZIP54 transcription factor, was strongly induced by cold stress. Haplotype analysis confirmed that +376 bp (T>A) in the OsbZIP54 coding region played a key role in regulating cold tolerance in rice. Conclusion We identified OsbZIP54 as a novel regulatory gene associated with rice cold-responsive traits, with its Dongfu-104 allele showing specific cold-induction expression serving as an important molecular variation for rice improvement. This result is expected to further exploration of the genetic mechanism of rice cold tolerance at the seedling stage and improve cold tolerance in rice varieties by marker-assisted selection.

scholarly journals Semi-Transparent Organic Photovoltaics Applied as Greenhouse Shade for Spring and Summer Tomato Production in Arid Climate

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1152
Author(s):  
Rebekah Waller ◽  
Murat Kacira ◽  
Esther Magadley ◽  
Meir Teitel ◽  
Ibrahim Yehia
Keyword(s):  
Solar Radiation ◽  
Organic Photovoltaics ◽  
Crop Production ◽  
Production Systems ◽  
High Light ◽  
Growth And Yield ◽  
Control Group ◽  
Tomato Crop ◽  
Tomato Production ◽  
Control Section

Recognizing the growing interest in the application of organic photovoltaics (OPVs) with greenhouse crop production systems, in this study we used flexible, roll-to-roll printed, semi-transparent OPV arrays as a roof shade for a greenhouse hydroponic tomato production system during a spring and summer production season in the arid southwestern U.S. The wavelength-selective OPV arrays were installed in a contiguous area on a section of the greenhouse roof, decreasing the transmittance of all solar radiation wavelengths and photosynthetically active radiation (PAR) wavelengths (400–700 nm) to the OPV-shaded area by approximately 40% and 37%, respectively. Microclimate conditions and tomato crop growth and yield parameters were measured in both the OPV-shaded (‘OPV’) and non-OPV-shaded (‘Control’) sections of the greenhouse. The OPV shade stabilized the canopy temperature during midday periods with the highest solar radiation intensities, performing the function of a conventional shading method. Although delayed fruit development and ripening in the OPV section resulted in lower total yields compared to the Control section (24.6 kg m−2 and 27.7 kg m−2, respectively), after the fourth (of 10 total) harvests, the average weekly yield, fruit number, and fruit mass were not significantly different between the treatment (OPV-shaded) and control group. Light use efficiency (LUE), defined as the ratio of total fruit yield to accumulated PAR received by the plant canopy, was nearly twice as high as the Control section, with 21.4 g of fruit per mole of PAR for plants in the OPV-covered section compared to 10.1 g in the Control section. Overall, this study demonstrated that the use of semi-transparent OPVs as a seasonal shade element for greenhouse production in a high-light region is feasible. However, a higher transmission of PAR and greater OPV device efficiency and durability could make OPV shades more economically viable, providing a desirable solution for co-located greenhouse crop production and renewable energy generation in hot and high-light intensity regions.

Sign in / Sign up

Export Citation Format

Share Document