scholarly journals Reproductive neuronal circuitry in adaptive changes of energy balance

2021 ◽  
Author(s):  
Pilhwa Lee ◽  
Cristina Sáenz de Miera ◽  
Nicole Bellefontaine ◽  
Marina A Silveira ◽  
Thais T Zampieri ◽  
...  
Keyword(s):  
Dopamine Transporter ◽  
Leptin Receptor ◽  
Prolactin Receptor ◽  
Reproductive Function ◽  
Late Pregnancy ◽  
High Energy ◽  
Metabolic Dysfunction ◽  
Adaptive Responses ◽  
New Findings ◽  
Pregnancy And Lactation

AbstractThe crosstalk between metabolism and reproduction is essential for species survival. When dysfunctional, this interaction may decrease reproductive efficiency, but in physiological conditions of high energy demands, e.g., pregnancy and lactation, it is highly beneficial. Females display adaptive responses that assure offspring survival and health, including increased food intake and suppression of the reproductive function. Some of these physiological responses are modulated by leptin actions in neuronal pathways that are still unclear. The hypothalamic ventral premammillary nucleus (PMv) is a key integrative node of metabolic cues and reproductive status, comprised of either leptin-depolarized or -hyperpolarized neurons. Here, we show that the subset of leptin-hyperpolarized neurons coexpresses dopamine transporter (DAT) and prolactin receptor. DAT expression is higher in prepubertal conditions, when reproductive function is suppressed. These neurons innervate AgRP presynaptic terminals and may potentiate their inhibitory actions on reproduction. We further applied a mathematical model to reconcile our new findings with the current literature and to verify if those neurons are putative components of the metabolic control of reproduction. In our model, leptin-depolarized PMv neurons project to and directly stimulate kisspeptin and gonadotropin releasing hormone (GnRH) neurons. Leptin-hyperpolarized PMv DAT neurons are directly stimulated by prolactin and project to inhibitory control sites. During conditions of high prolactin levels, i.e., late pregnancy and lactation, this pathway may overcome the former, facilitating AgRP actions in the suppression of the reproductive function. Our model also predicts that overstimulation of this pathway may underlie earlier puberty and reproductive deficits observed in conditions of metabolic dysfunction.Significance StatementWomen with excess or low energy stores (e.g., obesity or anorexia) have reproductive deficits, including altered puberty onset, disruption of reproductive cycles and decreased fertility. If able to conceive, they show higher risks of miscarriages and preterm birth. The hypothalamic circuitry controlling the interplay between metabolism and reproduction is undefined. Neurons in the ventral premammillary nucleus express leptin receptor and project to reproductive control sites. Those neurons are essentially glutamatergic, but functionally and phenotypically heterogeneous. They either depolarize or hyperpolarize in response to leptin. We show that leptin-hyperpolarized neurons coexpress dopamine transporter and prolactin receptor, and project to AgRP inhibitory output. Computational modeling was applied to build a neuronal network integrating metabolism and reproduction in typical and dysfunctional physiology.

scholarly journals Transcript abundances of the prolactin receptor, the leptin receptor and their major suppressor in the sheep mammary gland during pregnancy and lactation

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Malgorzata Szczesna ◽  
Katarzyna Kirsz ◽  
Michal Nowakowski ◽  
Dorota A. Zieba
Keyword(s):  
Mammary Gland ◽  
Leptin Receptor ◽  
Prolactin Receptor ◽  
Signal Transduction Pathway ◽  
Late Pregnancy ◽  
Transcript Level ◽  
Transcript Abundance ◽  
Cytokine Signaling ◽  
Pregnancy And Lactation ◽  
Plasma Prl

AbstractThis study aimed to expand the knowledge of the interactions between prolactin (PRL) and leptin in the ovine mammary gland during pregnancy and lactation; we examined the mRNA expression of prolactin receptor (PRLR), the long form of the leptin receptor (LRb) and suppressor of cytokine signaling (SOCS)-3 in mammary gland biopsies collected on days 60, 90 and 120 of pregnancy and on days 30, 60 and 90 of lactation (n = 6 for each time point), along with the plasma PRL and leptin concentrations. The PRL concentrations were stable throughout pregnancy and increased during lactation. The plasma leptin concentrations were comparable among nonpregnant, early-pregnant, late-pregnant and lactating ewes, but this metric peaked during mid-pregnancy. Expression of PRLR and SOCS-3 in the mammary gland fluctuated during the transition from pregnancy to lactation, and differences in LRb expression occurred during the late stages of lactation. The LRb transcript abundance was approximately 31 times higher in ewes on day 60 of lactation than in early-lactating ewes. Expression of SOCS-3 mRNA in biopsies gradually decreased over the course of pregnancy and reached a minimum value during late pregnancy. After lambing, the transcript level of SOCS-3 increased and peaked on day 60 of lactation. During pregnancy, the plasma PRL concentration positively correlated with the abundances of PRLR (r = 0.971, P < 0.01) and SOCS-3 (r = 0.818, P < 0.05). Positive correlations were also observed between the transcript abundances of SOCS-3 and LRb (r = 0.854, P < 0.05). The variations observed in the plasma PRL and leptin concentrations and the changes in expression of key leptin and PRL signal transduction pathway components, such as PRLR, LRb and SOCS-3, indicate that the efficacies of both hormone actions are modulated in a multilevel manner throughout pregnancy and lactation. These interactions may regulate the ability of the mammary gland to respond to current energy requirements and challenges, thus affecting milk yield and lactation duration.

scholarly journals Expression of a hypomorphic Pomc allele alters leptin dynamics during late pregnancy

2020 ◽  
Vol 245 (1) ◽  
pp. 115-127 ◽  
Author(s):  
Hui Yu ◽  
Zoe Thompson ◽  
Sylee Kiran ◽  
Graham L Jones ◽  
Lakshmi Mundada ◽  
...  
Keyword(s):  
Fat Mass ◽  
Energy Homeostasis ◽  
Leptin Receptor ◽  
Late Pregnancy ◽  
Placental Development ◽  
Hypomorphic Mutation ◽  
Protein Levels ◽  
Maximal Arc ◽  
Hypothalamic Arcuate Nucleus ◽  
Pregnancy And Lactation

Proopiomelanocortin (POMC) neurons in the hypothalamic arcuate nucleus (ARC) are essential for normal energy homeostasis. Maximal ARC Pomc transcription is dependent on neuronal Pomc enhancer 1 (nPE1), located 12 kb upstream from the promoter. Selective deletion of nPE1 in mice decreases ARC Pomc expression by 70%, sufficient to induce mild obesity. Because nPE1 is located exclusively in the genomes of placental mammals, we questioned whether its hypomorphic mutation would also alter placental Pomc expression and the metabolic adaptations associated with pregnancy and lactation. We assessed placental development, pup growth, circulating leptin and expression of Pomc, Agrp and alternatively spliced leptin receptor (LepR) isoforms in the ARC and placenta of Pomc∆1/∆1 and Pomc+/+ dams. Despite indistinguishable body weights, lean mass, food intake, placental histology and Pomc expression and overall pregnancy outcomes between the genotypes, Pomc ∆1/∆1 females had increased pre-pregnancy fat mass that paradoxically decreased to control levels by parturition. However, Pomc∆1/∆1 dams had exaggerated increases in circulating leptin, up to twice of that of the typically elevated levels in Pomc+/+ mice at the end of pregnancy, despite their equivalent fat mass. Pomc∆1/∆1dams also had increased placental expression of soluble leptin receptor (LepRe), although the protein levels of LEPRE in circulation were the same as Pomc+/+ controls. Together, these data suggest that the hypomorphic Pomc∆1/∆1 allele is responsible for the perinatal super hyperleptinemia of Pomc∆1/∆1 dams, possibly due to upregulated leptin secretion from individual adipocytes.

Expression of prolactin receptor (PRL-R) mRNA in the caudal gland of Hokkaido sika deer (Cervus nippon yesoensis Heude, 1884)

2001 ◽  
Vol 79 (3) ◽  
pp. 416-419
Author(s):  
Masatsugu Suzuki ◽  
Yuri Kamewaka ◽  
Masami Yamanaka ◽  
Toshihiko Iwanaga ◽  
Noriyuki Ohtaishi
Keyword(s):  
Cervus Elaphus ◽  
Sika Deer ◽  
Prolactin Receptor ◽  
Cervus Nippon ◽  
Late Pregnancy ◽  
Blood Levels ◽  
Hybridization Technique ◽  
Pregnancy And Lactation ◽  
Caudal Gland ◽  
Cervus Nippon Yesoensis

We applied the in situ hybridization technique to the Hokkaido sika deer (Cervus nippon yesoensis) caudal glands using probes designed from the red deer (Cervus elaphus) prolactin receptor cDNA. Prolactin receptor mRNA expression was consistently detected in the secretory epithelial cells collected from fawns and lactating hinds. This suggests that the presence of developed caudal glands during late pregnancy and lactation may be caused by increased blood levels of prolactin. During late pregnancy and lacation, visual range is limited for deer in forests and grasslands because of the flourishing leaves and sprigs. Therefore, we suggest that the scent emitted from the caudal gland in a particular season coordinates with and reinforces the visual alarm effect of the rump patch and tail.

scholarly journals Central growth hormone action regulates metabolism during pregnancy

2019 ◽  
Vol 317 (5) ◽  
pp. E925-E940 ◽  
Author(s):  
Pryscila D. S. Teixeira ◽  
Gisele C. Couto ◽  
Isadora C. Furigo ◽  
Edward O. List ◽  
John J. Kopchick ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Food Intake ◽  
Energy Homeostasis ◽  
Leptin Receptor ◽  
Late Pregnancy ◽  
Metabolic Adaptations ◽  
Growth Hormone Action ◽  
Lower Glucose ◽  
Pregnancy And Lactation ◽  
Glucose Stimulated Insulin Secretion

The maternal organism undergoes numerous metabolic adaptations to become prepared for the demands associated with the coming offspring. These metabolic adaptations involve changes induced by several hormones that act at multiple levels, ultimately influencing energy and glucose homeostasis during pregnancy and lactation. Previous studies have shown that central growth hormone (GH) action modulates glucose and energy homeostasis. However, whether central GH action regulates metabolism during pregnancy and lactation is still unknown. In the present study, we generated mice carrying ablation of GH receptor (GHR) in agouti-related protein (AgRP)–expressing neurons, in leptin receptor (LepR)–expressing cells or in the entire brain to investigate the role played by central GH action during pregnancy and lactation. AgRP-specific GHR ablation led to minor metabolic changes during pregnancy and lactation. However, while brain-specific GHR ablation reduced food intake and body adiposity during gestation, LepR GHR knockout (KO) mice exhibited increased leptin responsiveness in the ventromedial nucleus of the hypothalamus during late pregnancy, although their offspring showed reduced growth rate. Additionally, both Brain GHR KO and LepR GHR KO mice had lower glucose tolerance and glucose-stimulated insulin secretion during pregnancy, despite presenting increased insulin sensitivity, compared with control pregnant animals. Our findings revealed that during pregnancy central GH action regulates food intake, fat retention, as well as the sensitivity to insulin and leptin in a cell-specific manner. Together, the results suggest that GH acts in concert with other “gestational hormones” to prepare the maternal organism for the metabolic demands of the offspring.

scholarly journals Interaction of late pregnancy and lactation in rats

Reproduction ◽  
1999 ◽  
Vol 115 (2) ◽  
pp. 341-347 ◽  
Author(s):  
T. R. Koiter ◽  
H. Moes ◽  
N. Valkhof ◽  
S. Wijkstra
Keyword(s):  
Late Pregnancy ◽  
Pregnancy And Lactation

ANALYSIS OF RIBOSOMES AND POLYSOMES DERIVED FROM RABBIT MAMMARY GLANDS DURING PREGNANCY AND LACTATION

1971 ◽  
Vol 49 (4) ◽  
pp. 667-NP ◽  
Author(s):  
I. D. HERRIMAN ◽  
G. D. BAIRD ◽  
JUDY M. BRUCE
Keyword(s):  
Electron Microscopy ◽  
Density Gradient ◽  
Gradient Analysis ◽  
Late Pregnancy ◽  
Sucrose Density Gradient ◽  
Mammary Glands ◽  
Pregnancy And Lactation

SUMMARY Whole-ribosome and polysome-enriched fractions were prepared from the mammary glands of rabbits during late pregnancy and lactation. The composition of the fractions was determined by sucrose density gradient analysis and electron microscopy. The range of size of polysomal aggregates was similar in the late-pregnant and lactating gland, with aggregates containing five to nine ribosomal units predominating. However, the amount of polysomes relative to monosomes was invariably found to increase after parturition. The greater portion of this increase was accounted for by the increased abundance of aggregates containing five to nine units.

Influence of temperature on maternity roost selection by noctule bats (Nyctalus noctula) and Leisler’s bats (N. leisleri) in Białowieża Primeval Forest, Poland

2006 ◽  
Vol 84 (6) ◽  
pp. 900-907 ◽  
Author(s):  
Ireneusz Ruczyński
Keyword(s):  
Late Pregnancy ◽  
Ground Level ◽  
Data Logger ◽  
Primeval Forest ◽  
Tree Cavities ◽  
Roost Selection ◽  
Pregnancy And Lactation ◽  
Maximum Temperatures ◽  
Białowieża Primeval Forest ◽  
Nyctalus Noctula

This study tests whether the temperature of tree cavities determines their selection by bats in Białowieża Primeval Forest (BPF), eastern Poland. Using a data logger, I simultaneously measured the temperature in cavities selected by bats as roosts and the temperature in available but unselected cavities. The maternity roosts chosen by noctule bats, Nyctalus noctula (Schreber, 1774), and Leisler’s bats, N. leisleri (Kuhl, 1817), during late pregnancy and lactation were warmer than unoccupied cavities, but temperature ranges in the two types of cavity did not differ. A logistic regression model showed that the mean cavity temperature during the night (2200–0400) and the minimum cavity temperature over a 24 h period were crucial for roost selection. This suggests that female noctule and Leisler’s bats selected roosts that promoted juvenile growth and used tree cavities that could save them energy while they were active because the cavity temperatures were close to the lower critical temperature of their thermoneutral zone. I also suggest that selection of warmer cavities minimized the bats' energy expenditure prior to emergence from the roost and that passive rewarming inside the cavity was an important factor in minimizing energetic costs of roosting by bats in BPF. Mean and maximum temperatures recorded near tree trunks increased with the height at which the temperature was measured (ground level, 10, 20, and 30 m), suggesting that bats can gain thermal benefits from insolation of the trunk by selecting highly placed cavities, as was observed in BPF.

The effect of level and pattern of energy intake during late pregnancy on the performance of housed Scottish Blackface ewes

1973 ◽  
Vol 16 (2) ◽  
pp. 165-171 ◽  
Author(s):  
T. H. McClelland ◽  
T. J. Forbes
Keyword(s):  
Body Weight ◽  
Energy Intake ◽  
Post Partum ◽  
Body Weight Loss ◽  
Late Pregnancy ◽  
High Energy ◽  
Metabolizable Energy ◽  
Feeding Period ◽  
Total Period ◽  
Constant Energy

SUMMARYSixty Scottish Blackface ewes were used in a 2 × 2 factorial experiment in which two levels of metabolizable energy (ME) were given during the final 6 weeks of pregnancy. In two treatments 1600 and 2000 kcal M E were given daily over the total period while in the remaining treatments daily ME intakes were 1200 and 1600 kcal ME during the first 3 weeks of the feeding period and 2000 and 2400 kcal ME during the last 3 weeks. Digestible crude protein (DCP) intakes were constant at approximately 30 g per head daily in the constant energy treatments and 15 and 45 g per head daily in the first and second periods respectively for the low-high energy treatments.Energy intake had no statistically significant effect on lamb birth weight nor on ewe net body-weight change (change from the start of the experimental feeding period to immediately post partum). Ewes on low-high energy intakes had a significantly lower net body-weight loss than did ewes on constant energy intakes. Pattern of feeding had no significant effect on lamb birth weights. Negative nitrogen balances were found during the first feeding period where the daily DCP intake was approximately 15 g per head.

scholarly journals Pregnancy- and lactation-associated osteoporosis with vertebral fractures: a systematic review

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Ying Qian ◽  
Lei Wang ◽  
Lili Yu ◽  
Weimin Huang
Keyword(s):  
Back Pain ◽  
Vertebral Fractures ◽  
Data Extraction ◽  
Treatment Options ◽  
Osteoporotic Fractures ◽  
Late Pregnancy ◽  
Mean Value ◽  
Thoracolumbar Region ◽  
Pregnancy And Lactation ◽  
Z Scores

Abstract Background To review, analyze and characterize the pregnancy and lactation-related osteoporosis (PLO) with vertebral fractures based on the extraction data in the previous studies. Methods A comprehensive literature search of electronic databases including the PubMed, Embase and Web of Science was conducted from January 1st,1990 to December 1st, 2020. The enrolled data were pooled to analyze the baseline characteristics, clinical features, risk factors and treatment options. Results A total of 65 articles with 338 cases were enrolled for data extraction. The enrolled cases aged from 19 to 47 years, with a mean value of 35.7 years old. The average body mass index (BMI) was 22.2 kg/m2 ranged from 16.0 to 39.0 kg/m2. Of the 173 cases, 149 cases with vertebral fractures occurred in the first pregnancy, 19 cases in the second pregnancy, four cases in the third pregnancy and one case in the fourth pregnancy. Up to 91.5% of the back pain occurred within the last 3 months of pregnancy and the first 3 months after delivery. The most involved vertebral levels were L2, L1 and T12 accounting for 32.6% of all the fractures. The average fracture numbers were 4.4 levels per patient. The lumbar Z-scores were mostly recorded with a mean value of − 3.2 ranged from − 7.8 to 0. Conclusions PLO with vertebral fractures is a rare clinical entity, which is more likely to occur in older and thinner pregnant women. Back pain is the clinical complaint and mostly occurs in the late pregnancy and early lactation periods. Most vertebral fractures appear in the first pregnancy but it can occur in any time of pregnancy. Thoracolumbar region is the mostly involved region. As compared with postmenopausal osteoporotic fractures, PLO usually has multiple levels fractures. Bisphosphonates are the most widely used treatment so far, however, many factors need to be taken into account to decide which drug to choose in PLO and further studies are necessary for clear recommendation in the future.

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