scholarly journals Net charges of the ribosomal proteins of the S10 and spc clusters of halophiles are inversely related to the degree of halotolerance

2021 ◽  
Author(s):  
Madhan Tirumalai ◽  
Daniela Anane-Bediakoh ◽  
Siddharth Rajesh ◽  
George E. Fox
Keyword(s):  
Ribosomal Proteins ◽  
Halophilic Bacteria ◽  
General Rule ◽  
Halophilic Archaea ◽  
Bacterial Strains ◽  
Extremely Halophilic Archaea ◽  
The Individual ◽  
Net Charges ◽  
Halophilic Fungi ◽  
Positively Charged

Net positive charge(s) on ribosomal proteins (r-proteins) have been reported to influence the assembly and folding of ribosomes. A high percentage of r-proteins from extremely halophilic archaea are known to be acidic or even negatively charged. Those proteins that remain positively charged are typically far less so. Herein the analysis is extended to the non-archaeal halophilic bacteria, eukaryotes and halotolerant archaea. The net charges (pH 7.4) of r-proteins that comprise the S10-spc operon/cluster from individual microbial and eukaryotic genomes were estimated and intercompared. It was observed that as a general rule, as the salt tolerance of the bacterial strains increased from 5 to 15%, the net charges of the individual proteins remained mostly basic. The most striking exceptions were the extremely halophilic bacterial strains, Salinibacter ruber SD01, Acetohalobium arabaticum DSM 5501 and Selenihalanaerobacter shriftii ATCC BAA-73, which are reported to require a minimum of 18%-21% of salt for their growth. All three strains have a higher number of acidic S10-spc cluster r-proteins than what is seen in the moderate halophiles or the halotolerant strains. Of the individual proteins, only uL2 never became acidic. uS14 and uL16 also seldom became acidic. The net negative charges on several of the S10-spc cluster r-proteins is a feature generally shared by all extremely halophilic archaea and bacteria. The S10-spc cluster r-proteins of halophilic fungi and algae (eukaryotes) were exceptions. They were positively charged despite the halophilicity of the organisms.

scholarly journals Net Charges of the Ribosomal Proteins of the S10 and spc Clusters of Halophiles Are Inversely Related to the Degree of Halotolerance

2021 ◽  
Author(s):  
Madhan R. Tirumalai ◽  
Daniela Anane-Bediakoh ◽  
Sidharth Rajesh ◽  
George E. Fox
Keyword(s):  
Ribosomal Proteins ◽  
Inverse Relationship ◽  
Halophilic Bacteria ◽  
Net Charges ◽  
Positively Charged

The net charges (at pH 7.4) of the ribosomal proteins (r-proteins) that comprise the S10-spc cluster show an inverse relationship with the halophilicity/halotolerance levels in both bacteria and archaea. In non-halophilic bacteria, the S10-spc cluster r-proteins are generally basic (positively charged), while the rest of the proteomes in these strains are generally acidic.

Survival of extremely and moderately halophilic isolates of Tunisian solar salterns after UV-B or oxidative stress

2011 ◽  
Vol 57 (11) ◽  
pp. 923-933 ◽  
Author(s):  
Hana Trigui ◽  
Salma Masmoudi ◽  
Céline Brochier-Armanet ◽  
Sami Maalej ◽  
Sam Dukan
Keyword(s):  
Halophilic Bacteria ◽  
Halophilic Archaea ◽  
Oxygen Species ◽  
Solar Saltern ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Moderately Halophilic ◽  
Nacl Concentration ◽  
Extremely Halophilic Archaea ◽  
Moderately Halophilic Bacteria

Adaptation to a solar saltern environment requires mechanisms providing tolerance not only to salinity but also to UV radiation (UVR) and to reactive oxygen species (ROS). We cultivated prokaryote halophiles from two different salinity ponds: the concentrator M1 pond (240 g·L–1NaCl) and the crystallizer TS pond (380 g·L–1NaCl). We then estimated UV-B and hydrogen peroxide resistance according to the optimal salt concentration for growth of the isolates. We observed a higher biodiversity of bacterial isolates in M1 than in TS. All strains isolated from TS appeared to be extremely halophilic Archaea from the genus Halorubrum . Culturable strains isolated from M1 included extremely halophilic Archaea (genera Haloferax , Halobacterium , Haloterrigena , and Halorubrum) and moderately halophilic Bacteria (genera Halovibrio and Salicola ). We also found that archaeal strains were more resistant than bacterial strains to exposure to ROS and UV-B. All organisms tested were more resistant to UV-B exposure at the optimum NaCl concentration for their growth, which is not always the case for H2O2. Finally, if these results are extended to other prokaryotes present in a solar saltern, we could speculate that UVR has greater impact than ROS on the control of prokaryote biodiversity in a solar saltern.

Functional analysis of seven ribosomal systems from extremely halophilic archaea

1993 ◽  
Vol 39 (3) ◽  
pp. 311-317 ◽  
Author(s):  
José L. Sanz ◽  
Irma Marín ◽  
Dionisio Ureña ◽  
Ricardo Amils
Keyword(s):  
Functional Analysis ◽  
Protein Synthesis ◽  
Halophilic Archaea ◽  
Reference Systems ◽  
Protein Synthesis Inhibitors ◽  
Structural Specificity ◽  
Archaeal Protein ◽  
Extremely Halophilic Archaea ◽  
Positively Charged ◽  
Specific Inhibitors

The sensitivity of seven extremely halophilic archaeal protein synthesis systems to 42 inhibitors with different domain (kingdom), functional, and structural specificity has been studied. The results obtained underline the functional variability of archaeal ribosomes when compared with bacterial and eukaryotic reference systems. Halophilic ribosomes are sensitive to some of the specific inhibitors of bacteria, such as thiostrepton, carbomycin A, and griseoviridin, or of eukaryotes, such as anisomycin and bruceantin, and extremely sensitive to some of the universal inhibitors, such as puromycin and sparsomycin. Suitable control experiments allow us to postulate that some negative inhibitory results may be caused by competition between positively charged antibiotics and the high ionic conditions required for halophilic protein synthesis.Key words: protein synthesis inhibitors, halophilic archaea, ribosomes.

scholarly journals Synthesis and Characterization of a Bioartificial Polymeric System with Potential Antibacterial Activity: Chitosan-Polyvinyl Alcohol-Ampicillin

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3109 ◽  
Author(s):  
Andres Bernal-Ballen ◽  
Jorge Lopez-Garcia ◽  
Martha-Andrea Merchan-Merchan ◽  
Marian Lehocky
Keyword(s):  
Antibacterial Activity ◽  
Polyvinyl Alcohol ◽  
Statistical Significance ◽  
Antibacterial Properties ◽  
Intermolecular Forces ◽  
Polymeric System ◽  
Bacterial Strains ◽  
Natural Polymers ◽  
The Matrix ◽  
The Individual

Bio-artificial polymeric systems are a new class of polymeric constituents based on blends of synthetic and natural polymers, designed with the purpose of producing new materials that exhibit enhanced properties with respect to the individual components. In this frame, a combination of polyvinyl alcohol (PVA) and chitosan, blended with a widely used antibiotic, sodium ampicillin, has been developed showing a moderate behavior in terms of antibacterial properties. Thus, aqueous solutions of PVA at 1 wt.% were mixed with acid solutions of chitosan at 1 wt.%, followed by adding ampicillin ranging from 0.3 to 1.0 wt.% related to the total amount of the polymers. The prepared bio-artificial polymeric system was characterized by FTIR, SEM, DSC, contact angle measurements, antibacterial activity against Staphylococcus aureus and Escherichia coli and antibiotic release studies. The statistical significance of the antibacterial activity was determined using a multifactorial analysis of variance with ρ < 0.05 (ANOVA). The characterization techniques did not show alterations in the ampicillin structure and the interactions with polymers were limited to intermolecular forces. Therefore, the antibiotic was efficiently released from the matrix and its antibacterial activity was preserved. The system disclosed moderate antibacterial activity against bacterial strains without adding a high antibiotic concentration. The findings of this study suggest that the system may be effective against healthcare-associated infections, a promising view in the design of novel antimicrobial biomaterials potentially suitable for tissue engineering applications.

scholarly journals Can the relation between fat and protein in milk be changed by selective breeding

1947 ◽  
Vol 19 (1) ◽  
pp. 8-21
Author(s):  
T. Lonka
Keyword(s):  
Milk Yield ◽  
Climatic Factors ◽  
Production Capacity ◽  
General Rule ◽  
Regression Equation ◽  
Fat Percentage ◽  
Protein Percentage ◽  
Coefficient Of Correlation ◽  
Rule Set ◽  
The Individual

In Finland the protein content of the feeds is in general so low that it restricts the milk yield particularly in herds of high production capacity. This is especially significant for the reason that owing primarily to climatic factors the quality of the feeds cannot be notably improved in this respect. Therefore an effort should be made to change the composition of milk by means of breeding in such a way that it would correspond to the possibilities of feed cultivation in Finland better than at present. This would mean that it should be endeavoured to breed cows whose milk is very fat-rich but at the same time poor in protein, in which case more of the commercially valuable butterfat could be produced on the feed grown on the estate. Since a correspondingly greater amount of milk of poor protein percentage could be produced than that of protein rich milk, the breeding of such cows would not decrease the yield of protein, which is of such great food value, but as great an amount in kilograms of milk protein would be available as earlier for domestic purposes as well as dairies. We have aimed at this goal in our country in general by endeavouring to raise the fat percentage of the milk by means of breeding. It is known that protein percentage does not increase exactly as markedly as the fat percentage, wherefore the protein yield per kilogram of fat decreases as the percentage rises. The influence of breeding would however be incomparably greater if low protein percentage were selected simultaneously with high fat percentage. In the investigation we have dealt with the possibilities of such selection in Finnish cattle, in connection with which we have striven to make clear how much the protein percentage of the milk varies independently of the fat percentage. The material comprises 54 West-Finnish Native cows; the fat and protein percentages of their milk yield has been determined once a month during one lactation. The relation of fat and protein is not the same throughout the whole lactation, but it changes so that at the end of the lactation the protein percentage as compared with the fat percentage is relatively greater than at the beginning of the period (Table and Figure 1). The averages of the fat and protein percentages and likewise the correlation between them depend consequently upon the length of the calving interval. Therefore the said averages have been estimated only on the basis of 2—7 production months. Fy means of these averages r = +0.60 ± 0.09 was derived as the coefficient of correlation between the fat and protein percentages y = 1.53 + 0.39 x as the regression equation, x = fat percentage and y = protein percentage. The individual cows diverge very much from the general rule set by the regression equation, which can be concluded already from the comparatively low value of the coefficient of correlation. The protein percentage of the milk yield of the West- Finnish Native cows diverges at least about one per cent independently of the fat percentage when judging on the basis of the extreme divergencies in the material. Although a part of the divergency evidently is caused by external factors, the role of the genetic factors is nevertheless so great that there appear to be great possibilities for breeding cows producing fat-rich milk by means of breeding but having a protein which would be considerably lower than the present average.

scholarly journals Production, characterization, and antitumor efficiency of l-glutaminase from halophilic bacteria

2022 ◽  
Vol 46 (1) ◽  
Author(s):  
Eman Zakaria Gomaa
Keyword(s):  
Cell Lines ◽  
Halophilic Bacteria ◽  
Temperature Stability ◽  
Inoculum Size ◽  
Dependent Manner ◽  
Bacterial Strains ◽  
Carcinoma Cell Lines ◽  
Initial Ph ◽  
Hct 116 ◽  
Optimization Of Fermentation

Abstract Background Halophiles are an excellent source of enzymes that are not only salt stable, but also can withstand and carry out reaction efficiently under extreme conditions. l-glutaminase has attracted much attention with respect to proposed applications in several fields such as pharmaceuticals and food industries. The aim of the present study was to investigate the anticancer activity of l-glutaminase produced by halophilic bacteria. Various halophilic bacterial strains were screened for extracellular l-glutaminase production. An attempt was made to study the optimization, purification, and characterization of l-glutaminase from Bacillus sp. DV2-37. The antitumor activity of the produced enzyme was also investigated. Results The potentiality of 15 halophilic bacterial strains isolated from the marine environment that produced extracellular l-glutaminase was investigated. Bacillus sp. DV2-37 was selected as the most potent strain and optimized for enzyme production. The optimization of fermentation process revealed that the highest enzyme activity (47.12 U/ml) was observed in a medium supplemented with 1% (w/v) glucose as a carbon source, 1% (w/v) peptone as a nitrogen source, 5% (w/v) NaCl, the initial pH was 7.0, at 37 °C, using 20% (v/v) inoculum size after 96 h of incubation. The produced crude enzyme was partially purified by ammonium sulfate precipitation and dialysis. Of the various parameters tested, pH 7, 40 °C, and 5% NaCl were found to be the best for l-glutaminase activity. The enzyme also exhibited high salt and temperature stability. The antitumor effect against human breast (MCF-7), hepatocellular (HepG-2), and colon (HCT-116) carcinoma cell lines revealed that l-glutaminase produced by Bacillus sp. DV2-37 showed potent cytotoxic activity of all the tested cell lines in a dose-dependent manner with an IC50 value of 3.5, 3.4, and 3.8 µg/ml, respectively. Conclusions The present study proved that l-glutaminase produced by marine bacteria holds proper features and it has a high potential to be useful for many therapeutic applications.

scholarly journals Methylomes of Two Extremely Halophilic Archaea Species, Haloarcula marismortui and Haloferax mediterranei

2019 ◽  
Vol 8 (27) ◽  
Author(s):  
Shiladitya DasSarma ◽  
Alexey Fomenkov ◽  
Satyajit L. DasSarma ◽  
Tamas Vincze ◽  
Priya DasSarma ◽  
...  
Keyword(s):  
Single Molecule ◽  
Gene Annotation ◽  
Halophilic Archaea ◽  
Restriction Enzymes ◽  
Haloferax Mediterranei ◽  
Haloarcula Marismortui ◽  
Content Type ◽  
Extremely Halophilic Archaea ◽  
Large Plasmids ◽  
Methyl Cytosine

ABSTRACT The genomes of two extremely halophilic Archaea species, Haloarcula marismortui and Haloferax mediterranei, were sequenced using single-molecule real-time sequencing. The ∼4-Mbp genomes are GC rich with multiple large plasmids and two 4-methyl-cytosine patterns. Methyl transferases were incorporated into the Restriction Enzymes Database (REBASE), and gene annotation was incorporated into the Haloarchaeal Genomes Database (HaloWeb).

scholarly journals Genome Sequences and Methylation Patterns of Natrinema versiforme BOL5-4 and Natrinema pallidum BOL6-1, Two Extremely Halophilic Archaea from a Bolivian Salt Mine

2019 ◽  
Vol 8 (33) ◽  
Author(s):  
Priya DasSarma ◽  
Brian P. Anton ◽  
Satyajit L. DasSarma ◽  
Fabiana L. Martinez ◽  
Daniel Guzman ◽  
...  
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Genome Annotation ◽  
Halophilic Archaea ◽  
Salt Mine ◽  
Genome Sequences ◽  
Content Type ◽  
Extremely Halophilic Archaea ◽  
Methylation Patterns

Two extremely halophilic archaea, namely, Natrinema versiforme BOL5-4 and Natrinema pallidum BOL6-1, were isolated from a Bolivian salt mine and their genomes sequenced using single-molecule real-time sequencing. The GC-rich genomes of BOL5-4 and BOL6-1 were 4.6 and 3.8 Mbp, respectively, with large chromosomes and multiple megaplasmids. Genome annotation was incorporated into HaloWeb and methylation patterns incorporated into REBASE.

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