scholarly journals A mechanistic investigation of enhanced nitrogen use efficiency in wheat seedlings after treatment with an Ascophyllum nodosum biostimulant

2021 ◽  
Author(s):  
Łukasz Łangowski ◽  
Oscar Goñi ◽  
Elomofe Ikuyinminu ◽  
Ewan Feeney ◽  
Shane O’Connell

ABSTRACTReduction in the emissions of the greenhouse gas nitrous oxide and nitrogen (N) pollution of ground water by improving nitrogen use efficiency (NUE) in crops is urgently required in pursuit of a sustainable agricultural future. Utilising an engineered biostimulant (PSI-362) derived from the brown seaweed Ascophyllum nodosum, we examined its effect on wheat seedling growth dynamics and mechanistic spatiotemporal changes at transcriptional and biochemical levels in relation to N uptake, assimilation and NUE. PSI-362-mediated biomass increase was associated with increased nitrate uptake and N assimilation in the form of glutamate, glutamine, free amino acids, soluble proteins and total chlorophyll. Phenotypical and biochemical analysis were supported by evaluation of differential expression of genetic markers involved in nitrate perception and transport (TaNRT1.1/NPF6.3), and assimilation (TaNR1 and TaNiR1, TaGDH2, TaGoGAT, TaGS1). Finally, a comparative analysis of the PSI-362 and two generic Ascophyllum nodosum extracts (ANEs) demonstrated that the NUE effect greatly differs depending on the ANE biostimulant used. In the current context of climate warming the transition of agriculture to a more sustainable model is urgently required. Application and adoption of precision biostimulants creates an opportunity for sustainable crop management, reduced production cost and environmental pollution, while maintaining yields.

Author(s):  
Eugenio Cozzolino ◽  
Ida Di Mola ◽  
Lucia Ottaiano ◽  
Christophe El-Nakhel ◽  
Youssef Rouphael ◽  
...  

Highlights- The effects of three plant-based biostimulants on yield and quality of processing tomato was explored.- Application of protein hydrolysates and seaweed extract improve marketable yield.- The biostimulants had different effect on nutritional and functional quality of tomato.- Hydrophilic antioxidant activity and ascorbic acid content increased under protein hydrolysate application.   Tomato (Solanum lycopersicum L.) is a diffused worldwide vegetable. Great amounts of fertilizers are often applied for increasing yield and quality, without considering the negative effect on the environment. A possible perspective for reducing this risk is to raise the nitrogen use efficiency (NUE) through the use of plant biostimulants, which also improve yield and quality concomitantly. The aim of the current study was to verify the potential beneficial effect of three vegetal-based biostimulants on agronomical, qualitative and nitrogen use efficiency of a processing tomato crop. The experiment provided three biostimulants (an extract of brown seaweed [SwE], a legume-derived protein hydrolysate [LDPH] and a tropical plant extract). The following assessments were carried out: marketable and unmarketable yields, mean fruits weight, firmness, pH, total soluble solids (TSS), color parameters (a/b), hydrophilic antioxidant activity (HAA), lipophilic antioxidant activity (LAA), total ascorbic acid content (AsA), total phenols, nitrate and total nitrogen content, nitrogen use efficiency, N-uptake efficiency, and N-utilization. The foliar application of biostimulants especially protein hydrolysates and seaweed extract significantly affected the marketable yield with an average increase of 18.3% over the control and 41.3% average decrease in unmarketable yield. The N-use and N-uptake efficiency followed a similar trend, with biostimulants boosting it higher than control, +18.4% and +59.3%, respectively; the nitrogen content was also higher in fruits of sprayed plants: + 21.3% over control. This finding also reflects on higher dry matter accumulation and firmness in fruits of treated plants (+10.9% and +14.1% over control, respectively). The biostimulants application, in particular SwE and LDPH, also boosted TSS (+12.8%), the a/b color ratio (+7.5%), HAA and AsA (9.8% and 114.6%, respectively). Therefore, the legume-derived protein hydrolysates and extract of brown seaweed Ecklonia maxima seem a good sustainable approach to improve yield and quality of tomato for canning industries.


2018 ◽  
Vol 5 (3) ◽  
pp. 79-88
Author(s):  
Chairunnisak Chairunnisak ◽  
Sugiyanta Sugiyanta ◽  
Edi Santosa

Nitrogen use efficiency (NUE) is a necessitate in order to enhance sustainable rice farming in Indonesia. Thus, objective of present research was to evaluate NUE of local and national Indonesian superior aromatic rice treated with different levels of nitrogen fertilizer (N). Planting plot was arranged using five levels of N as the main plot, i.e; 0, 45, 90, 135 and 180 kg ha-1; and two rice varieties as subplot, i.e: Sigupai Abdya (local) and Inpari 23 Bantul (national). The results showed application 180 kg N ha-1 to Sigupai  Abdya significantly increased the plant height. However, it also postponed the flowering time. Inpari 23 Bantul treated with 180 kg N ha-1 produced the highest number of tillers. Combination of 90 kg N ha-1 with Sigupai Abdya variety significantly reduced the number of empty grains. Sigupai Abdya variety has a higher number of grains per panicle and sampling plot yield than Inpari 23 Bantul, and  dosage 90 kg N ha-1 increases grain yield per clump also sampling plot yield significantly. Nitrogen at 180 kg N ha-1 made Sigupai Abdya variety has high N content and absorption N in primordia phase, and the Inpari 23 Bantul variety had grain with high N content. Nitrogen at 90 kg ha-1 caused Sigupai Abdya variety at primordia phase had NUE higher than Inpari 23 Bantul. This study showed that local variety Sigupai Abdya is suitable for development as rice with low input NKeywords: Aceh aromatic, low input, nitrogen dose, N uptake, Oryza sativa L. 


2021 ◽  
Vol 23 (1) ◽  
pp. 50
Author(s):  
Wilda Lumban Tobing ◽  
Mariani Sembiring

<p>The expansion of oil palm plantations continues to increase so that it requires quality seed. Nurseries need to be done before moving to the field. The use of Azotobacter and cocoa fruit skin compost is one way to increase the  growth and nitrogen use efficiency in oil palm in main nursery. This research was aimed to know the growth and nitrogen use efficiency of palm oil in main nursery. This research was conducted on the research area of the Agricultural Agribusiness College of Agriculture Practices (STIPAP) Medan and the Laboratory of Balai Pengkajian Teknologi Pertanian (BPTP) of North Sumatra and the Agricultural Laboratory of the University of North Sumatera from February until Juli 2013. The method used was Randomized Group Design factorial with 3 replications and followed by Duncan test at α=5%. The first factor is Azotobacter including without giving Azotobacter (A0), 20 ml/polybag (A1) and 40 ml/polybag (A2). The second factor is cocoa fruit skin compost, which consists of 3 of them, namely without compost (K0), 125 g/polybag (K1), and 250 g/polybag (K2). The research parameters were plant dry weight (g), nitrogen uptake (mg) and N use efficiency (EPN). The results showed that the use of Azotobacter and cocoa fruit skin compost were able to significantly increasing dry weight of plant and N uptake of oil palm seeds and gave the highest EPN value of 12.93.  </p>


2020 ◽  
Vol 12 (21) ◽  
pp. 8780
Author(s):  
Muhammad Muhaymin Mohd Zuki ◽  
Noraini Md. Jaafar ◽  
Siti Zaharah Sakimin ◽  
Mohd Khanif Yusop

Nitrogen (N) fertilizer is commonly used to supply sufficient N for plant uptake, for which urea is one of the highly preferred synthetic N fertilizers due to its high N content. Unfortunately, N provided by urea is rapidly lost upon urea application to soils through ammonia volatilization, leaching, and denitrification. Thus, treatment of urea with urease inhibitor (N-(n-Butyl) Thiophosphoric Triamide (NBPT)) is among the solutions to slow down urea hydrolysis, therefore reducing loss of NH3 and saving N available for plant uptake and growth. A field study was carried out to evaluate the effects of NBPT-coated urea (NCU) at varying rates on growth, yield, and nitrogen use efficiency (NUE) of maize in tropical soil. The experiment was conducted at Field 15, Universiti Putra Malaysia, Serdang, Selangor, Malaysia, and maize (Zea mays var. Thai Super Sweet) was used as the test crop. The results showed that all maize grown in soils applied with urea coated with NBPT (NCU) (T2, T3, T4, and T5) had significantly (P ≤ 0.05) higher chlorophyll content compared to the control (T0 and T1). The surface leaf area of maize grown in NCU-treated soils at 120 kg N h−1 (T3) was recorded as the highest. NCU at and 96 kg N ha−1 (T3 and T4) were relatively effective in increasing maize plant dry weight, yield, and N uptake. Improvement of NUE by 45% over urea was recorded in the treatment of NCU at 96 kg N ha−1. NBPT-coated urea (NCU) at 96 kg N ha−1 had potential to increase the growth, yield, nitrogen uptake, and NUE of maize by increasing the availability of N for plant growth and development.


Author(s):  
A.K. Dhaka ◽  
Satish Kumar ◽  
Bhagat Singh ◽  
Karmal Singh ◽  
Amit Kumar ◽  
...  

An experiment was conducted to study nitrogen use efficiency in pigeonpea at Research farm, CCS Haryana Agricultural University, Hisar, India having three nipping treatments (no nipping, nipping at just start of branching and nipping at flower initiation) and five fertility levels (control, 20 kg N + 40 kg P2O5/ha, 30 kg N + 40 kg P2O5 /ha, 40 kg N + 40 kg P2O5/ha and 20 kg N + 40 kg P2O5/ha + foliar spray of 2% N immediately after nipping) replicated thrice in split plot design during growing seasons of 2016 and 2017. Nipping at start of branching reduced the plant height, while increased primary and secondary branches, pods/plant and yield over no nipping. Significantly higher total N uptake, protein content, net return, B: C, agronomical NUE, physiologic NUE, agro-physiologic NUE, apparent recovery efficiency, utilization efficiency of N and partial N balance were improved with nipping at start of branching. Among fertility levels, 40 kg N + 40 kg P2O5 / ha recorded significantly higher yield attributes with 39.7 per cent higher seed yield over control. Significantly higher agronomic NUE, physiologic NUE, agro-physiological NUE, apparent recovery efficiency, utilization efficiency of N, partial N balance and NER were recorded with 20 kg/ha as compared to higher nitrogen doses.


Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1157
Author(s):  
Lawrence Aula ◽  
Peter Omara ◽  
Eva Nambi ◽  
Fikayo B. Oyebiyi ◽  
William R. Raun

Improvement of nitrogen use efficiency (NUE) via active optical sensors has gained attention in recent decades, with the focus of optimizing nitrogen (N) input while simultaneously sustaining crop yields. To the authors’ knowledge, a comprehensive review of the literature on how optical sensors have impacted winter wheat (Triticum aestivum L.) NUE and grain yield has not yet been performed. This work reviewed and documented the extent to which the use of optical sensors has impacted winter wheat NUE and yield. Two N management approaches were evaluated; optical sensor and conventional methods. The study included 26 peer-reviewed articles with data on NUE and grain yield. In articles without NUE values but in which grain N was included, the difference method was employed to compute NUE based on grain N uptake. Using optical sensors resulted in an average NUE of 42% (±2.8% standard error). This approach improved NUE by approximately 10.4% (±2.3%) when compared to the conventional method. Grain yield was similar for both approaches of N management. Optical sensors could save as much as 53 (±16) kg N ha−1. This gain alone may not be adequate for increased adoption, and further refinement of the optical sensor robustness, possibly by including weather variables alongside sound agronomic management practices, may be necessary.


Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 941
Author(s):  
Roxana Vidican ◽  
Anamaria Mălinaș ◽  
Ioan Rotar ◽  
Rozalia Kadar ◽  
Valeria Deac ◽  
...  

Nitrogen fertilization is indispensable in increasing wheat crop productivity but, in order to achieve maximum profitable production and minimum negative environmental impact, improving nitrogen use efficiency (NUE) should be considered. The aim of this study was to evaluate the nitrogen use efficiency (NUE) in a long-term wheat–maize–soybean rotation system with the final purpose of increasing the overall performance of the wheat cropping system. Research was undertaken at the Agricultural Research Development Station Turda (ARDS Turda), located in Western Transylvania Plain, Romania. The experimental field was carried out at a fixed place during seven wheat vegetation seasons. The plant material consisted of a wheat variety created by the ARDS Turda (Andrada), one variety of maize (Turda 332) and one variety of soybean (Felix). The experiment covered two planting patterns: wheat after maize and wheat after soybean and five levels of nitrogen fertilization (control-unfertilized, fertilization with 0—control plot, 30, 60, 90 and 120 kg N ha−1 y−1). The following indices were assessed: NUE (nitrogen use efficiency), N uptake and PFP (partial factor productivity). The results of the present study suggest that reduced N-fertilization doses could improve N uptake and utilization for both planting patterns.


2007 ◽  
Vol 55 (3) ◽  
pp. 383-391 ◽  
Author(s):  
M. Venugopalan ◽  
K. Hebbar ◽  
P. Tiwary ◽  
S. Chatterji ◽  
V. Ramamurthy ◽  
...  

A field experiment was conducted under rainfed conditions, on a shallow soil (Inceptisol) underlain with weathered basalt and on a deep soil (Vertisol) to evaluate three cotton cultivars [AKH 4 ( Gossypium arboreum ), LRK 516 ( G. hirsutum ) and NHH 44 (intra- hirsutum hybrid)] under four levels of N (0, 40, 80 and 120 kg ha −1 ) and to analyse the variations in productivity using the nitrogen use efficiency (NUE) parameter. The yield of AKH4 and NHH 44 was 101 and 89% higher than that of LRK 516. The yield and the response to N were higher on the Inceptisol. The enhanced yield and NUE of AKH 4 and NHH 44 were attributed to the improved efficiency of N uptake utilization. NUE declined from 21.6 at 40 kg ha −1 to 7.7 at 120 kg N ha −1 . The N uptake efficiency and N utilization efficiency were independent of each other, but complemented each other in improving NUE. The implications of variations in NUE, N uptake efficiency and N utilization efficiency and their components, N biomass production efficiency and HI, in cotton breeding and agronomy are also discussed.


1998 ◽  
Vol 78 (4) ◽  
pp. 611-613 ◽  
Author(s):  
R. Michaud ◽  
G. Bélanger ◽  
A. Brégard ◽  
J. Surprenant

Improvement of N use efficiency of timothy (Phleum pratense L.) involves higher DM yield but, for feeding purposes, N concentration should also be considered. Differences in N use efficiency were found among 40 genotypes of timothy. The analysis of residues of the linear regression between N uptake and DM yield of all genotypes allowed for the selection of genotypes with both high N use efficiency and contrasted N concentrations. Key words: Phleum pratense L., timothy, genetic selection, nitrogen use efficiency


2017 ◽  
Vol 54 (4) ◽  
pp. 577-591 ◽  
Author(s):  
VIJAY POONIYA ◽  
YASHBIR SINGH SHIVAY ◽  
MADAN PAL ◽  
RADHIKA BANSAL

SUMMARYDeficiencies of micro (Zn, B) and secondary (S) nutrients are well-documented in soil as well as in cereal crops, leading to decreased crop yields and low nutritional quality of food. We evaluated the effects of coated urea on maize yield, N and Zn uptake, and input cost relationships. Field experiments were conducted on maize to test boron-coated (BCU), sulphur-coated (SCU) and zinc-coated urea (ZnCU) during 2013 and 2014. On the basis of 2 years’ average data, the highest grain yield was achieved with 0.5% BCU, 5% SCU and 2.5% ZnCU as zinc sulphate hepta hydrate. These treatments increased yields by 5.4, 12.8 and 9.9% over prilled urea (PU), respectively. Application of 0.5% BCU (supplying 1.4 kg B ha−1), 5% SCU (supplying 14.1 kg S ha−1) and 2.5% ZnCU (supplying 7.05 kg Zn ha−1) registered the highest N concentrations and uptake in grain and stover. Total N uptake (grain + stover) was increased by 7.6, 16.7 and 17.1% with BCU, SCU and ZnCU treatments over PU. As compared to PU, Zn concentration in maize grain was significantly higher and total Zn uptake (grain + stover) increased by 32.4% with 2.5% ZnCU. Coated urea materials also enhanced the partial factor productivity (PFPN), agronomic efficiency (AEN), recovery efficiency (REN) and harvest index (HIN) over those of PU. From the economic viewpoint this study suggests that coating of urea with 0.3% boron, 5% sulphur or 2% zinc gives maximum net returns and benefit-cost ratio. Our data indicate that coating of B, Zn and S onto urea increases maize yield, profitability and nitrogen use efficiency in the western Indo-Gangetic plains of India.


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