Hydrogen sulfide promotes nodulation and nitrogen fixation in soybean-rhizobia symbiotic system

AbstractThe rhizobium-legume symbiotic system is crucial for nitrogen cycle balance in agriculture. Hydrogen sulfide (H2S), a gaseous signaling molecule, may regulate various physiological processes in plants. However, whether H2S has regulatory effect in this symbiotic system remains unknown. Herein, we investigated the possible role of H2S in the symbiosis between soybean (Glycine max) and rhizobium (Sinorhizobium fredii). Our results demonstrated that exogenous H2S donor (sodium hydrosulfide, NaHS) treatment promoted soybean growth, nodulation and nitrogenase (Nase) activity. Western blotting analysis revealed that the abundance of nitrogenase component nifH was increased by NaHS treatment in nodules. Quantitative real-time PCR data showed that NaHS treatment up-regulated the expressions of symbiosis-related genesnodCandnodDofS. fredii. Besides, expression of soybean nodulation marker genes including early nodulin 40 (GmENOD40), ERF required for nodulation (GmERN), nodulation signaling pathway2b (GmNSP2b) and nodulation inception genes (GmNIN1a, GmNIN2aandGmNIN2b) were up-regulated. Moreover, the expressions of glutamate synthase (GmGS), nitrite reductase (GmNiR), ammonia transporter (GmSAT1), andnifHinvolved in nitrogen metabolism were up-regulated in NaHS-treated soybean roots and nodules. Together, our results suggested that H2S may act as a positive signaling molecule in soybean-rhizobia symbiotic system and enhance their nitrogen fixation ability.HighlightWe demonstrated for the first time that H2S as a signaling molecule may promote the establishment of symbiotic relationship and nitrogen fixation ability in the soybean-rhizobia symbiotic system.

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Hydrogen Sulfide Promotes Nodulation and Nitrogen Fixation in Soybean–Rhizobia Symbiotic System

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-01-19-0003-r ◽

2019 ◽

Vol 32 (8) ◽

pp. 972-985 ◽

Cited By ~ 7

Author(s):

Hang Zou ◽

Ni-Na Zhang ◽

Qing Pan ◽

Jian-Hua Zhang ◽

Juan Chen ◽

...

Keyword(s):

Nitrogen Fixation ◽

Hydrogen Sulfide ◽

Glutamate Synthase ◽

Marker Genes ◽

Symbiotic System ◽

Signaling Molecule ◽

Sodium Hydrosulfide ◽

Physiological Processes ◽

Soybean Rhizobia ◽

Early Nodulin

The rhizobium–legume symbiotic system is crucial for nitrogen cycle balance in agriculture. Hydrogen sulfide (H2S), a gaseous signaling molecule, may regulate various physiological processes in plants. However, whether H2S has regulatory effect in this symbiotic system remains unknown. Herein, we investigated the possible role of H2S in the symbiosis between soybean (Glycine max) and rhizobium (Sinorhizobium fredii). Our results demonstrated that an exogenous H2S donor (sodium hydrosulfide [NaHS]) treatment promoted soybean growth, nodulation, and nitrogenase (Nase) activity. Western blotting analysis revealed that the abundance of Nase component nifH was increased by NaHS treatment in nodules. Quantitative real-time polymerase chain reaction data showed that NaHS treatment upregulated the expressions of symbiosis-related genes nodA, nodC, and nodD of S. fredii. In addition, expression of soybean nodulation marker genes, including early nodulin 40 (GmENOD40), ERF required for nodulation (GmERN), nodulation signaling pathway 2b (GmNSP2b), and nodulation inception genes (GmNIN1a, GmNIN2a, and GmNIN2b), were upregulated. Moreover, the expressions of glutamate synthase (GmGOGAT), asparagine synthase (GmAS), nitrite reductase (GmNiR), ammonia transporter (GmSAT1), leghemoglobin (GmLb), and nifH involved in nitrogen metabolism were upregulated in NaHS-treated soybean roots and nodules. Together, our results suggested that H2S may act as a positive signaling molecule in the soybean–rhizobia symbiotic system and enhance the system’s nitrogen fixation ability.

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Hydrogen Sulfide Inhibits High Glucose-Induced sFlt-1 Production via Decreasing ADAM17 Expression in 3T3-L1 Adipocytes

International Journal of Endocrinology ◽

10.1155/2017/9501792 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

Tian-xiao Hu ◽

Gang Wang ◽

Wei Wu ◽

Lu Gao ◽

Qing-ying Tan ◽

...

Keyword(s):

Hydrogen Sulfide ◽

High Glucose ◽

Vascular Complications ◽

Signaling Molecule ◽

Sodium Hydrosulfide ◽

Diabetic Vascular Complications ◽

Subsequent Decrease ◽

Therapeutic Value

Hydrogen sulfide (H2S) has recently been identified as an endogenous gaseous signaling molecule. The aim of the present study was to investigate the effect of H2S on high glucose- (HG-) induced ADAM17 expression and sFlt-1 production in 3T3-L1 adipocytes. Firstly, we found that HG DMEM upregulated the expression of ADAM17 and production of sFlt-1 in 3T3-L1 adipocytes. Knocking down ADAM17 attenuated the effect of high glucose on sFlt-1 production in adipocytes. HG decreased the expression of CSE and 3-MST, as well as the endogenous H2S production. Furthermore, knocking down CSE and 3-MST significantly increased ADAM17 expression and sFlt-1 production. The addition of exogenous H2S through the administration of sodium hydrosulfide (NaHS) inhibited HG-induced upregulation of ADAM17 expression and sFlt-1 production. In conclusion, decreased expression of CSE and 3-MST and the subsequent decrease in H2S production contribute to high glucose-induced sFlt-1 production via activating ADAM17 in adipocytes. Exogenous H2S donor NaHS has a potential therapeutic value for diabetic vascular complications.

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A fluorescent turn-on H2S-responsive probe: design, synthesis and application

Organic & Biomolecular Chemistry ◽

10.1039/c5ob01305d ◽

2015 ◽

Vol 13 (38) ◽

pp. 9760-9766 ◽

Cited By ~ 28

Author(s):

Yufeng Zhang ◽

Haiyan Chen ◽

Dan Chen ◽

Di Wu ◽

Xiaoqiang Chen ◽

...

Keyword(s):

Hydrogen Sulfide ◽

Insulin Secretion ◽

Ischemia Reperfusion ◽

Probe Design ◽

Signaling Molecule ◽

Design Synthesis ◽

The Third ◽

Turn On ◽

Physiological Processes

Hydrogen sulfide (H2S) is considered as the third signaling moleculein vivoand it plays an important role in various physiological processes and pathological processesin vivo, such as vasodilation, apoptosis, neurotransmission, ischemia/reperfusion-induced injury, insulin secretion and inflammation.

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Hydrogen sulfide reduces serum triglyceride by activating liver autophagy via the AMPK-mTOR pathway

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.00294.2015 ◽

2015 ◽

Vol 309 (11) ◽

pp. E925-E935 ◽

Cited By ~ 56

Author(s):

Li Sun ◽

Song Zhang ◽

Chengyuan Yu ◽

Zhenwei Pan ◽

Yang Liu ◽

...

Keyword(s):

Hydrogen Sulfide ◽

High Fat Diet ◽

Serum Triglyceride ◽

Mtor Pathway ◽

Autophagic Flux ◽

High Fat ◽

Sodium Hydrosulfide ◽

Metabolism Inhibition ◽

Qualitative Effect ◽

First Time

Autophagy plays an important role in liver triglyceride (TG) metabolism. Inhibition of autophagy could reduce the clearance of TG in the liver. Hydrogen sulfide (H2S) is a potent stimulator of autophagic flux. Recent studies showed H2S is protective against hypertriglyceridemia (HTG) and noalcoholic fatty liver disease (NAFLD), while the mechanism remains to be explored. Here, we tested the hypothesis that H2S reduces serum TG level and ameliorates NAFLD by stimulating liver autophagic flux by the AMPK-mTOR pathway. The level of serum H2S in patients with HTG was lower than that of control subjects. Sodium hydrosulfide (NaHS, H2S donor) markedly reduced serum TG levels of male C57BL/6 mice fed a high-fat diet (HFD), which was abolished by coadministration of chloroquine (CQ), an inhibitor of autophagic flux. In HFD mice, administration of NaSH increased the LC3BII-to-LC3BI ratio and decreased the p62 protein level. Meanwhile, NaSH increased the phosphorylation of AMPK and thus reduced the phosphorylation of mTOR in a Western blot study. In cultured LO2 cells, high-fat treatment reduced the ratio of LC3BII to LC3BI and the phosphorylation of AMPK, which were reversed by the coadministration of NaSH. Knockdown of AMPK by siRNA in LO2 cells blocked the autophagic enhancing effects of NaSH. The same qualitative effect was observed in AMPKα2−/− mice. These results for the first time demonstrated that H2S could reduce serum TG level and ameliorate NAFLD by activating liver autophagy via the AMPK-mTOR pathway.

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Physiological Implications of Hydrogen Sulfide in Plants: Pleasant Exploration behind Its Unpleasant Odour

Oxidative Medicine and Cellular Longevity ◽

10.1155/2015/397502 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 41

Author(s):

Zhuping Jin ◽

Yanxi Pei

Keyword(s):

Hydrogen Sulfide ◽

Growth And Development ◽

Defense Responses ◽

Plant Responses ◽

Plant Growth And Development ◽

Systematic Classification ◽

Physiological Processes ◽

Overwhelming Evidence

Recently, overwhelming evidence has proven that hydrogen sulfide (H2S), which was identified as a gasotransmitter in animals, plays important roles in diverse physiological processes in plants as well. With the discovery and systematic classification of the enzymes producing H2Sin vivo, a better understanding of the mechanisms by which H2S influences plant responses to various stimuli was reached. There are many functions of H2S, including the modulation of defense responses and plant growth and development, as well as the regulation of senescence and maturation. Additionally, mounting evidence indicates that H2S signaling interacts with plant hormones, hydrogen peroxide, nitric oxide, carbon monoxide, and other molecules in signaling pathways.

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Moving Past Quinone-Methides: Recent Advances toward Minimizing Electrophilic Byproducts from COS/H2S Donors

Current Topics in Medicinal Chemistry ◽

10.2174/1568026621666210622130002 ◽

2021 ◽

Vol 21 ◽

Author(s):

Michael Pluth

Keyword(s):

Hydrogen Sulfide ◽

Carbonic Anhydrase ◽

Therapeutic Potential ◽

Carbonyl Sulfide ◽

Quinone Methide ◽

Quinone Methides ◽

On Demand ◽

Physiological Processes ◽

Recent Approach ◽

Alternative Approaches

: Hydrogen sulfide (H2S) is an important biomolecule that plays key signaling and protective roles in different physiological processes. With the goals of advancing both the available research tools and the associated therapeutic potential of H2S, researchers have developed different methods to deliver H2S on-demand in different biological contexts. A recent approach to develop such donors has been to design compounds that release carbonyl sulfide (COS), which is quickly converted to H2S in biological systems by the ubiquitous enzyme carbonic anhydrase (CA). Although highly diversifiable, many approaches using this general platform release quinone methides or related electrophiles after donor activation. Many such electrophiles are likely scavenged by water, but recent efforts have also expanded alternative approaches that minimize the formation of electrophilic byproducts generated after COS release. This mini-review focuses specifically on recent examples of COS-based H2S donors that do not generate quinone methide byproducts after donor activation.

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Involvement of l-Cysteine Desulfhydrase and Hydrogen Sulfide in Glutathione-Induced Tolerance to Salinity by Accelerating Ascorbate-Glutathione Cycle and Glyoxalase System in Capsicum

Antioxidants ◽

10.3390/antiox9070603 ◽

2020 ◽

Vol 9 (7) ◽

pp. 603

Author(s):

Cengiz Kaya ◽

Bernardo Murillo-Amador ◽

Muhammad Ashraf

Keyword(s):

Hydrogen Sulfide ◽

Liquid Solution ◽

Sweet Pepper ◽

Monodehydroascorbate Reductase ◽

Glyoxalase System ◽

Sodium Hydrosulfide ◽

Chlorophyll Contents ◽

Cysteine Desulfhydrase ◽

Induced Tolerance ◽

Tolerance To Salinity

The aim of this study is to assess the role of l-cysteine desulfhydrase (l-DES) and endogenous hydrogen sulfide (H2S) in glutathione (GSH)-induced tolerance to salinity stress (SS) in sweet pepper (Capsicum annuum L.). Two weeks after germination, before initiating SS, half of the pepper seedlings were retained for 12 h in a liquid solution containing H2S scavenger, hypotaurine (HT), or the l-DES inhibitor dl-propargylglycine (PAG). The seedlings were then exposed for three weeks to control or SS (100 mmol L−1 NaCl) and supplemented with or without GSH or GSH+NaHS (sodium hydrosulfide, H2S donor). Salinity suppressed dry biomass, leaf water potential, chlorophyll contents, maximum quantum efficiency, ascorbate, and the activities of dehydroascorbate reductase, monodehydroascorbate reductase, and glyoxalase II in plants. Contrarily, it enhanced the accumulation of hydrogen peroxide, malondialdehyde, methylglyoxal, electrolyte leakage, proline, GSH, the activities of glutathione reductase, peroxidase, catalase, superoxide dismutase, ascorbate peroxidase, glyoxalase I, and l-DES, as well as endogenous H2S content. Salinity enhanced leaf Na+ but reduced K+; however, the reverse was true with GSH application. Overall, the treatments, GSH and GSH+NaHS, effectively reversed the oxidative stress and upregulated salt tolerance in pepper plants by controlling the activities of the AsA-GSH and glyoxalase-system-related enzymes as well as the levels of osmolytes.

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Magnesium Hydride-Mediated Sustainable Hydrogen Supply Prolongs the Vase Life of Cut Carnation Flowers via Hydrogen Sulfide

Frontiers in Plant Science ◽

10.3389/fpls.2020.595376 ◽

2020 ◽

Vol 11 ◽

Author(s):

Longna Li ◽

Yuhao Liu ◽

Shu Wang ◽

Jianxin Zou ◽

Wenjiang Ding ◽

...

Keyword(s):

Hydrogen Sulfide ◽

Hydrogen Generation ◽

Magnesium Hydride ◽

Hydrogen Gas ◽

Vase Life ◽

Buffer Solution ◽

Citrate Buffer ◽

Sodium Hydrosulfide ◽

Practical Applications ◽

Citrate Buffer Solution

Magnesium hydride (MgH2) is a promising solid-state hydrogen source with high storage capacity (7.6 wt%). Although it is recently established that MgH2 has potential applications in medicine because it sustainably supplies hydrogen gas (H2), the biological functions of MgH2 in plants have not been observed yet. Also, the slow reaction kinetics restricts its practical applications. In this report, MgH2 (98% purity; 0.5–25 μm size) was firstly used as a hydrogen generation source for postharvest preservation of flowers. Compared with the direct hydrolysis of MgH2 in water, the efficiency of hydrogen production from MgH2 hydrolysis could be greatly improved when the citrate buffer solution is introduced. These results were further confirmed in the flower vase experiment by showing higher efficiency in increasing the production and the residence time of H2 in solution, compared with hydrogen-rich water. Mimicking the response of hydrogen-rich water and sodium hydrosulfide (a hydrogen sulfide donor), subsequent experiments discovered that MgH2-citrate buffer solution not only stimulated hydrogen sulfide (H2S) synthesis but also significantly prolonged the vase life of cut carnation flowers. Meanwhile, redox homeostasis was reestablished, and the increased transcripts of representative senescence-associated genes, including DcbGal and DcGST1, were partly abolished. By contrast, the discussed responses were obviously blocked by the inhibition of endogenous H2S with hypotaurine, an H2S scavenger. These results clearly revealed that MgH2-supplying H2 could prolong the vase life of cut carnation flowers via H2S signaling, and our results, therefore, open a new window for the possible application of hydrogen-releasing materials in agriculture.

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Intra-articular injection of hydrogen sulfide decreased the progression of gonarthrosis

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2018-0574 ◽

2019 ◽

Vol 97 (1) ◽

pp. 47-54 ◽

Cited By ~ 1

Author(s):

Kürşad Aytekin ◽

Selma Şengiz Erhan ◽

Züleyha Erişgin ◽

Cem Zeki Esenyel ◽

Selçuk Takır

Keyword(s):

Anterior Cruciate Ligament ◽

Hydrogen Sulfide ◽

Synovial Fluid ◽

Scoring System ◽

Cruciate Ligament ◽

The Other ◽

Sodium Hydrosulfide ◽

Medial Meniscectomy ◽

Anterior Cruciate

Hydrogen sulfide (H2S) is found in both the plasma and synovial fluid of patients with gonarthrosis. In the present study, we investigated whether intra-articular injection of sodium hydrosulfide (NaSH) (1 mM, 30 μL), a H2S donor, might affect gonarthrosis in rats. Gonarthrosis was induced surgically in the left knees of rats and left for 6 weeks for the development of disease. Then, intra-articular injections of NaSH or methylprednisolone (1 mg/kg, 30 μL) were administered to rats. Half of each group was sacrificed at the end of the first day and the other half was sacrificed at the end of 4 weeks to evaluate early and later effects of injections on gonarthrosis. The injury induced by anterior cruciate ligament resection and medial meniscectomy in rats caused the development of gonarthrosis. As the duration lengthened after gonarthrosis induction, the progression of the disease continued. According to the modified Mankin Scoring System, intra-articular injection of NaSH histopathologically slowed the progression of gonarthrosis, whereas methylprednisolone was ineffective. In addition, NaSH decreased apoptosis in rat knees with gonarthrosis. Each treatment did not cause injury to healthy knees. Our results lead to the consideration that intra-articular NaSH administration may be effective in the progression of gonarthrosis.

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