scholarly journals Intravital optoacoustic ultrasound bio-microscopy reveals radiation-inhibited skull angiogenesis

2018 ◽  
Author(s):  
Héctor Estrada ◽  
Johannes Rebling ◽  
Wolfgang Sievert ◽  
Daniela Hladik ◽  
Urs Hofmann ◽  
...  
Keyword(s):  
Large Scale ◽  
Bone Repair ◽  
Bone Development ◽  
Vascular Networks ◽  
Vascular Morphology ◽  
Complex Interactions ◽  
Vessel Remodeling ◽  
Imaging Approach ◽  
Microscopy Techniques ◽  
Transcranial Imaging

AbstractAngiogenesis is critical in bone development and growth. Dense, large-scale, and multi-layered vascular networks formed by thin-walled sinusoidal vessels perfuse the plate bones and play an important role in bone repair. Yet, the intricate functional morphology of skull microvasculature remains poorly understood as it is difficult to visualize using existing intravital microscopy techniques. Here we introduced an intravital fully-transcranial imaging approach based on hybrid optoacoustic and ultrasound bio-microscopy, allowing for large-scale observations and quantitative analysis of the vascular morphology, angiogenesis, vessel remodeling, and subsurface roughness in murine skulls. Our approach also enabled high-throughput physiological studies to understand radiation-inhibited angiogenesis in the skull bone. We observed previously undocumented sinusoidal vascular networks spanning the entire skullcap, thus opening new vistas for studying the complex interactions between calvarian, pial, and cortical vascular systems.

scholarly journals Rapid synchronized fabrication of vascularized thermosets and composites

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Mayank Garg ◽  
Jia En Aw ◽  
Xiang Zhang ◽  
Polette J. Centellas ◽  
Leon M. Dean ◽  
...  
Keyword(s):  
Large Scale ◽  
Capillary Flow ◽  
External Heat ◽  
Frontal Polymerization ◽  
Self Healing ◽  
Vascular Networks ◽  
Ambient Conditions ◽  
External Energy ◽  
Imaging Phantoms ◽  
Vascular Structures

AbstractBioinspired vascular networks transport heat and mass in hydrogels, microfluidic devices, self-healing and self-cooling structures, filters, and flow batteries. Lengthy, multistep fabrication processes involving solvents, external heat, and vacuum hinder large-scale application of vascular networks in structural materials. Here, we report the rapid (seconds to minutes), scalable, and synchronized fabrication of vascular thermosets and fiber-reinforced composites under ambient conditions. The exothermic frontal polymerization (FP) of a liquid or gelled resin facilitates coordinated depolymerization of an embedded sacrificial template to create host structures with high-fidelity interconnected microchannels. The chemical energy released during matrix polymerization eliminates the need for a sustained external heat source and greatly reduces external energy consumption for processing. Programming the rate of depolymerization of the sacrificial thermoplastic to match the kinetics of FP has the potential to significantly expedite the fabrication of vascular structures with extended lifetimes, microreactors, and imaging phantoms for understanding capillary flow in biological systems.

Changes in mechanisms and intensity of Western U.S. floods, 1960-2013

2021 ◽  
Author(s):  
Jessica Fayne ◽  
Huilin Huang ◽  
Mike Fischella ◽  
Yufei Liu ◽  
Zhaoxin Ban ◽  
...  
Keyword(s):  
Extreme Precipitation ◽  
Large Scale ◽  
Critical Factor ◽  
Flood Frequency ◽  
Atmospheric Rivers ◽  
Convective Storms ◽  
Noticeable Increase ◽  
Complex Interactions ◽  
Different Types ◽  
The Impact

<p>Extreme precipitation, a critical factor in flooding, has selectively increased with warmer temperatures in the Western U.S. Despite this, the streamflow measurements have captured no noticeable increase in large-scale flood frequency or intensity. As flood studies have mostly focused on specific flood events in particular areas, analyses of large-scale floods and their changes have been scarce. For floods during 1960-2013, we identify six flood generating mechanisms (FGMs) that are prominent across the Western U.S., including atmospheric rivers and non-atmospheric rivers, monsoons, convective storms, radiation-driven snowmelt, and rain-on-snow, in order to identify to what extent different types of floods are changing based on the dominant FGM. The inconsistency between extreme precipitation and lack of flood increase suggests that the impact of climate change on flood risk has been modulated by hydro-meteorological and physiographic processes such as sharp increases in temperature that drive increased evapotranspiration and decreased soil moisture. Our results emphasize the importance of FGMs in understanding the complex interactions of flooding and climatic changes and explain the broad spatiotemporal changes that have occurred across the vast Western U.S. for the past 50 years.</p>

Exploring host-pathogen interactions at the epithelial surface: application of transcriptomics in lung biology

2007 ◽  
Vol 292 (2) ◽  
pp. L367-L377 ◽  
Author(s):  
Joost B. Vos ◽  
Nicole A. Datson ◽  
Klaus F. Rabe ◽  
Pieter S. Hiemstra
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Large Scale ◽  
Brain Research ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Host Pathogen Interactions ◽  
Epithelial Surface ◽  
Complex Interactions ◽  
Host Pathogen

The epithelial surface of the airways is the largest barrier-forming interface between the human body and the outside world. It is now well recognized that, at this strategic position, airway epithelial cells play an eminent role in host defense by recognizing and responding to microbial exposure. Conversely, inhaled microorganisms also respond to contact with epithelial cells. Our understanding of this cross talk is limited, requiring sophisticated experimental approaches to analyze these complex interactions. High-throughput technologies, such as DNA microarray analysis and serial analysis of gene expression (SAGE), have been developed to screen for gene expression levels at large scale within single experiments. Since their introduction, these hypothesis-generating technologies have been widely used in diverse areas such as oncology and brain research. Successful application of these genomics-based technologies has also revealed novel insights in host-pathogen interactions in both the host and pathogen. This review aims to provide an overview of the SAGE and microarray technology illustrated by their application in the analysis of host-pathogen interactions. In particular, the interactions between epithelial cells in the human lungs and clinically relevant microorganisms are the central focus of this review.

Flow Patterns Downstream of a Mechanical Heart Valve During Systole

2007 ◽  
Author(s):  
P. Oshkai ◽  
F. Haji-Esmaeili
Keyword(s):  
Turbulent Flow ◽  
Heart Valve ◽  
Large Scale ◽  
Cardiac Cycle ◽  
Mechanical Heart Valve ◽  
Opening Angle ◽  
Image Velocimetry ◽  
Imaging Approach ◽  
Systolic Phase ◽  
Flow Through

Digital particle image velocimetry is employed to study turbulent flow through a bileaflet mechanical heart valve during systolic phase of a cardiac cycle. Unsteady vortex shedding from the valve’s leaflets displays distinct characteristic frequencies, depending on the opening angle of each leaflet. Small- and large-scale transverse oscillations of the separated shear layers are studied using global quantitative flow imaging approach. Turbulent flow structures including jet-like regions and shed vortices are characterized in terms of patterns of instantaneous and time-averaged velocity, vorticity, and turbulence statistics.

scholarly journals Functional Relationship between Osteogenesis and Angiogenesis in Tissue Regeneration

2020 ◽  
Vol 21 (9) ◽  
pp. 3242 ◽  
Author(s):  
Francesca Diomede ◽  
Guya Diletta Marconi ◽  
Luigia Fonticoli ◽  
Jacopo Pizzicanella ◽  
Ilaria Merciaro ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Growth Factors ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Development ◽  
Bone Substitutes ◽  
Primary Role ◽  
Vascular Networks ◽  
Tissue Restoration

Bone tissue renewal can be outlined as a complicated mechanism centered on the interaction between osteogenic and angiogenic events capable of leading to bone formation and tissue renovation. The achievement or debacle of bone regeneration is focused on the primary role of vascularization occurrence; in particular, the turning point is the opportunity to vascularize the bulk scaffolds, in order to deliver enough nutrients, growth factors, minerals and oxygen for tissue restoration. The optimal scaffolds should ensure the development of vascular networks to warrant a positive suitable microenvironment for tissue engineering and renewal. Vascular Endothelial Growth Factor (VEGF), a main player in angiogenesis, is capable of provoking the migration and proliferation of endothelial cells and indirectly stimulating osteogenesis, through the regulation of the osteogenic growth factors released and through paracrine signaling. For this reason, we concentrated our attention on two principal groups involved in the renewal of bone tissue defects: the cells and the scaffold that should guarantee an effective vascularization process. The application of Mesenchymal Stem Cells (MSCs), an excellent cell source for tissue restoration, evidences a crucial role in tissue engineering and bone development strategies. This review aims to provide an overview of the intimate connection between blood vessels and bone formation that appear during bone regeneration when MSCs, their secretome—Extracellular Vesicles (EVs) and microRNAs (miRNAs) —and bone substitutes are used in combination.

MJO-Induced Variability of the Diurnal Cycle of Precipitation over the Maritime Continent

2020 ◽  
Author(s):  
Ajda Savarin ◽  
Shuyi Chen
Keyword(s):  
Diurnal Cycle ◽  
Large Scale ◽  
Complex Nature ◽  
Madden Julian Oscillation ◽  
Maritime Continent ◽  
Convective Envelope ◽  
Modes Of Variability ◽  
Complex Interactions ◽  
The Indian Ocean ◽  
The Western Pacific

<p>Large-scale convection associated with the Madden-Julian Oscillation (MJO) initiates over the Indian Ocean and propagates eastward across the Maritime Continent (MC) into the western Pacific. As an MJO enters the MC, it often weakens or completely dissipates due to complex interactions between the large-scale MJO and the MC landmass and its topography. This is referred to as the MC barrier effect, and it is responsible for the dissipation of 40-50% of observed MJO events. One of the main reasons for the MJO’s weakening and dissipation over the MC is the diurnal cycle (DC), one of the strongest modes of variability in the region. Due to the complex nature of the MJO and the MC’s complicated topography, the interaction between the DC and the MJO is not well understood.</p><p>In this study, we examine the MJO-induced variability of the DC of precipitation over the MC. We use gridded satellite precipitation products (TRMM 3B42 and GPM IMERG) to: (1) track the MJO convective envelope using the Large-scale Precipitation Tracking algorithm (LPT), (2) analyze the changes in the DC of precipitation over the MC relative to the passage of the MJO. We find that the presence of an MJO not only increases the amount of precipitation over the MC, but that the increase is more pronounced over water than over land. The results from observations are compared to those from two reanalysis datasets (ERA5, MERRA-2). The reanalysis datasets are then used to examine the dynamic and thermodynamic changes that drive the variability in the DC of precipitation relative to the MJO. In addition, we separate MJO events into two groups based on whether they cross the MC, and independently examine their influences on the evolution of the DC of precipitation.</p>

Developmental Dynamics in Real Time

2009 ◽  
Vol 17 (3) ◽  
pp. 3-5
Author(s):  
Stephen W. Carmichael
Keyword(s):  
Real Time ◽  
Quantitative Methods ◽  
Migration Patterns ◽  
New Methods ◽  
Complex Interactions ◽  
Imaging Approach ◽  
Static Images ◽  
Embryologic Development ◽  
Time Periods ◽  
Developmental Dynamics

Embryologic development is a dynamic process that has been previously studied by examining static (usually chemically-fixed) specimens at different time periods and then extrapolating results by assembling a series of static images. Recently, Amy McMahon, Willy Supatto, Scott Fraser, and Angelike Stathopoulos have developed new methods to look at developmental migration patterns in real time. They used an optimized imaging approach and quantitative methods to analyze a two hour period during which gastrulation occurred in the embryos of fruitflies (Drosophila). Specifically, they characterized the complex interactions between cells of the ectoderm and mesoderm by tracking the movements of over 1,500 cells, which involved the analysis of over 100,000 cell positions for each embryo!

scholarly journals Humanized large-scale expanded endothelial colony–forming cells function in vitro and in vivo

Blood ◽  
2009 ◽  
Vol 113 (26) ◽  
pp. 6716-6725 ◽  
Author(s):  
Andreas Reinisch ◽  
Nicole A. Hofmann ◽  
Anna C. Obenauf ◽  
Karl Kashofer ◽  
Eva Rohde ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Large Scale ◽  
Proliferative Potential ◽  
Vascular Networks ◽  
Endothelial Progenitor ◽  
Endothelial Colony Forming Cells ◽  
Promising Tool

Abstract Endothelial progenitor cells are critically involved in essential biologic processes, such as vascular homeostasis, regeneration, and tumor angiogenesis. Endothelial colony–forming cells (ECFCs) are endothelial progenitor cells with robust proliferative potential. Their profound vessel-forming capacity makes them a promising tool for innovative experimental, diagnostic, and therapeutic strategies. Efficient and safe methods for their isolation and expansion are presently lacking. Based on the previously established efficacy of animal serum–free large-scale clinical-grade propagation of mesenchymal stromal cells, we hypothesized that endothelial lineage cells may also be propagated efficiently following a comparable strategy. Here we demonstrate that human ECFCs can be recovered directly from unmanipulated whole blood. A novel large-scale animal protein-free humanized expansion strategy preserves the progenitor hierarchy with sustained proliferation potential of more than 30 population doublings. By applying large-scale propagated ECFCs in various test systems, we observed vascular networks in vitro and perfused vessels in vivo. After large-scale expansion and cryopreservation phenotype, function, proliferation, and genomic stability were maintained. For the first time, proliferative, functional, and storable ECFCs propagated under humanized conditions can be explored in terms of their therapeutic applicability and risk profile.

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