scholarly journals A Na2CO3-responsive chitinase gene from Chinese wildrye improve pathogen resistance and saline-alkali stress tolerance in transgenic tobacco and maize

2019 ◽  
Author(s):  
Xiang-Guo Liu ◽  
Ying Yu ◽  
Qing Liu ◽  
Suren Deng ◽  
Xue-Bo Jin ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Pathogen Resistance ◽  
Chitinase Gene ◽  
Plant Performance ◽  
Growth And Yield ◽  
Neutral Salt ◽  
Alkali Stress ◽  
Alkaline Salt

AbstractSalinity and microbial pathogens are the major limiting factors for crop production. Although the manipulation of many genes could improve plant performance under either of these stresses, few genes have reported that could improve both pathogen resistance and saline-alkali stress tolerance. In this study, we identified a new chitinase gene CHITINASE 2 (LcCHI2) that encodes a class II chitinase from a Chinese wildrye (Leymus Chinensis), which grows naturally on alkaline-sodic soil. Overexpression of LcCHI2 increased chitinase activity in transgenic plants. The transgenic tobacco and maize exhibited improved pathogen resistance and enhanced both neutral salt and alkaline salt stress tolerance. Overexpression of LcCHI2 reduced sodium (Na+) accumulation, malondialdehyde content and relative electrical conductivity in transgenic tobacco under salt stress. In addition, the transgenic tobacco showed diminished lesion against bacterial and fungal pathogen challenge, suggesting an improved disease resistance. Similar improved performance was also observed in LcCHI2-overexpressed maize under both pathogen and salt stresses. It is worth noting that this genetic manipulation does not impair the growth and yield of transgenic tobacco and maize under normal cultivation condition. Apparently, application of LcCHI2 provides a new train of thought for genetically engineering saline-alkali and pathogen resistant crops of both dicots and monocots.

scholarly journals Genome-Wide Mining and Identification of Protein Kinase Gene Family Impacts Salinity Stress Tolerance in Highly Dense Genetic Map Developed from Interspecific Cross between G. hirsutum L. and G. darwinii G. Watt

Agronomy ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 560 ◽  
Author(s):  
Muhammad Shehzad ◽  
Zhongli Zhou ◽  
Allah Ditta ◽  
Xiaoyan Cai ◽  
Majid Khan ◽  
...  
Keyword(s):  
Salt Stress ◽  
Gene Family ◽  
Stress Tolerance ◽  
Genetic Map ◽  
Interspecific Cross ◽  
Genetic Erosion ◽  
Growth And Yield ◽  
Limiting Factor ◽  
Kinase Gene ◽  
Cotton Species

Abiotic stress is an important limiting factor in crop growth and yield around the world. Owing to the continued genetic erosion of the upland cotton germplasm due to intense selection and inbreeding, attention has shifted towards wild cotton progenitors which offer unique traits that can be introgressed into the cultivated cotton to improve their genetic performance. The purpose of this study was to characterize the Pkinase gene family in a previously developed genetic map of the F2 population derived from a cross between two cotton species: Gossypium hirsutum (CCRI 12-4) and Gossypium darwinii (5-7). Based on phylogenetic analysis, Pkinase (PF00069) was found to be the dominant domain with 151 genes in three cotton species, categorized into 13 subfamilies. Structure analysis of G. hirsutum genes showed that a greater percentage of genes and their exons were highly conserved within the group. Syntenic analysis of gene blocks revealed 99 duplicated genes among G. hirsutum, Gossypium arboreum and Gossypium raimondii. Most of the genes were duplicated in segmental pattern. Expression pattern analysis showed that the Pkinase gene family possessed species-level variation in induction to salinity and G. darwinii had higher expression levels as compared to G. hirsutum. Based on RNA sequence analysis and preliminary RT-qPCR verification, we hypothesized that the Pkinase gene family, regulated by transcription factors (TFs) and miRNAs, might play key roles in salt stress tolerance. These findings inferred comprehensive information on possible structure and function of Pkinase gene family in cotton under salt stress.

Overexpression of SlMDHAR in transgenic tobacco increased salt stress tolerance involving S-nitrosylation regulation

Plant Science ◽  
2020 ◽  
Vol 299 ◽  
pp. 110609 ◽  
Author(s):  
Qi Qi ◽  
Dong Yanyan ◽  
Liang Yuanlin ◽  
Li Kunzhi ◽  
Xu Huini ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Salt Stress Tolerance

scholarly journals The Potentiality of Marine Macro-Algae as Bio-Fertilizers to Improve the Productivity and Salt Stress Tolerance of Canola (Brassica napus L.) Plants

Agronomy ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 146 ◽  
Author(s):  
Hashem H.A. ◽  
Mansour H.A. ◽  
El-Khawas S.A. ◽  
Hassanein R.A.
Keyword(s):  
Salt Stress ◽  
Brassica Napus ◽  
Stress Tolerance ◽  
Growth And Yield ◽  
Phytochemical Analysis ◽  
Salt Stress Tolerance ◽  
Brassica Napus L ◽  
Indole Butyric Acid ◽  
Total Carbohydrates ◽  
The Impact

The present study aimed to evaluate the potentiality of three seaweeds, which belong to different algal taxa (green alga Ulva lactuca Linnaeus, brown alga Cystoseira spp., and red alga Gelidium crinale (Hare ex Turner) Gaillon) as bio-fertilizers to improve the growth and yield of canola (Brassica napus L.) plants under greenhouse conditions. Furthermore, the impact of seaweeds in alleviating the effects of salt stress (75 and 150 mM NaCl) on canola plants was also investigated. The three examined seaweeds (applied as soil amendments) successfully alleviated the harmful effects of salinity on canola plants by significantly reducing the inhibition of chlorophyll a, b, total carbohydrate accumulation, and growth promoting hormones, while increasing antioxidative compounds, such as phenols, flavonoids, anthocyanin, and osmoprotectants, including total carbohydrates and proline. Phytochemical analysis of the three examined seaweeds suggests that their stimulatory effect on growth and productivity under normal and salinity growth conditions may be linked to their constituents of a wide variety of growth promotive hormones, including indole acetic acid, indole butyric acid, gibberellic acid, cytokinins, total carbohydrates, and phenolic compounds. U. lactuca was found to be the best candidate to be used as a bio-fertilizer to improve canola growth, yield, and salt stress tolerance.

scholarly journals TaCIPK29, a CBL-Interacting Protein Kinase Gene from Wheat, Confers Salt Stress Tolerance in Transgenic Tobacco

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e69881 ◽  
Author(s):  
Xiaomin Deng ◽  
Wei Hu ◽  
Shuya Wei ◽  
Shiyi Zhou ◽  
Fan Zhang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Protein Kinase ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Salt Stress Tolerance ◽  
Kinase Gene ◽  
Interacting Protein ◽  
Protein Kinase Gene

scholarly journals Coordinated bacterial and plant sulfur metabolism in Enterobacter sp. SA187–induced plant salt stress tolerance

2021 ◽  
Vol 118 (46) ◽  
pp. e2107417118
Author(s):  
Cristina Andrés-Barrao ◽  
Hanin Alzubaidy ◽  
Rewaa Jalal ◽  
Kiruthiga G. Mariappan ◽  
Axel de Zélicourt ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Sulfur Metabolism ◽  
Endophytic Bacterium ◽  
Growth And Yield ◽  
Common Mechanism ◽  
Salt Stress Tolerance ◽  
Bacterial Gene ◽  
Bacterial Gene Expression ◽  
Flagellar Biosynthesis

Enterobacter sp. SA187 is a root endophytic bacterium that maintains growth and yield of plants under abiotic stress conditions. In this work, we compared the metabolic wirings of Arabidopsis and SA187 in the free-living and endophytic interaction states. The interaction of SA187 with Arabidopsis induced massive changes in bacterial gene expression for chemotaxis, flagellar biosynthesis, quorum sensing, and biofilm formation. Besides modification of the bacterial carbon and energy metabolism, various nutrient and metabolite transporters and the entire sulfur pathway were up-regulated. Under salt stress, Arabidopsis resembled plants under sulfate starvation but not when colonized by SA187, which reprogramed the sulfur regulon of Arabidopsis. In accordance, salt hypersensitivity of multiple Arabidopsis sulfur metabolism mutants was partially or completely rescued by SA187 as much as by the addition of sulfate, L-cysteine, or L-methionine. Many components of the sulfur metabolism that are localized in the chloroplast were partially rescued by SA187. Finally, salt-induced accumulation of reactive oxygen species as well as the hypersensitivity of LSU mutants were suppressed by SA187. LSUs encode a central regulator linking sulfur metabolism to chloroplast superoxide dismutase activity. The coordinated regulation of the sulfur metabolic pathways in both the beneficial microorganism and the host plant is required for salt stress tolerance in Arabidopsis and might be a common mechanism utilized by different beneficial microbes to mitigate the harmful effects of different abiotic stresses on plants.

scholarly journals Meta-analysis of the effect of expression of MYB transcription factor genes on abiotic stress

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11268
Author(s):  
Zhaolan Han ◽  
Xiaowen Shang ◽  
Lingxia Shao ◽  
Ya Wang ◽  
Xujun Zhu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Meta Analysis ◽  
Plant Performance ◽  
Myb Transcription Factor ◽  
Published Data ◽  
Experimental Conditions ◽  
Donor Type

Background MYB proteins are a large group of transcription factors. The overexpression of MYB genes has been reported to improve abiotic stress tolerance in plant. However, due to the variety of plant species studied and the types of gene donors/recipients, along with different experimental conditions, it is difficult to interpret the roles of MYB in abiotic stress tolerance from published data. Methods Using meta-analysis approach, we investigated the plant characteristics involved in cold, drought, and salt stress in MYB-overexpressing plants and analyzed the degrees of influence on plant performance by experimental variables. Results The results show that two of the four measured plant parameters in cold-stressed plants, two of the six in drought-stressed, and four of the 13 in salt-stressed were significantly impacted by MYB overexpression by 22% or more, and the treatment medium, donor/recipient species, and donor type significantly influence the effects of MYB-overexpression on drought stress tolerance. Also, the donor/recipient species, donor type, and stress duration all significantly affected the extent of MYB-mediated salt stress tolerance. In summary, this study compiles and analyzes the data across studies to help us understand the complex interactions that dictate the efficacy of heterologous MYB expression designed for improved abiotic stress tolerance in plants.

scholarly journals The impacts of space mutation and host genotype on endophyte role in host perennial ryegrass stress tolerance

2021 ◽  
Author(s):  
Bihua Ma ◽  
Yang Luo ◽  
Pei Tian ◽  
Zhenjiang Chen ◽  
Chunjie Li ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Plant Height ◽  
Perennial Ryegrass ◽  
Plant Performance ◽  
Nutrient Elements ◽  
Host Genotype ◽  
Rational Distribution ◽  
Significant Difference ◽  
Consistent Performance

Abstract Aims This study reveal if host genotype, endophyte or space mutation have effects on host stress resistance and select the best performance genotype.Methods Stress tolerance of 8 different perennial ryegrass genotypes including endophyte infected (E+) and endophyte free (E-) with and without space mutation were evaluated. Four different treatments were established which included control {CK, 45% of relatively soil moisture content (RSWC)}, drought stress (D, 15% RSWC), salt stress (S, 250 mmol NaCl with 45% RSWC) and drought combined with salt stress (DS, 250 mmol NaCl with 15% RSWC).Results The results showed that stress treatments significantly inhibited (P< 0.05) the growth of perennial ryegrass as plant height, tiller numbers and biomass of most plants significantly decreased (P<0.05). Under both control and stress treatments, host genotypes had significant (P<0.05) effects on plant growth, contents of phytohormones, ion and nutrient elements. However, there is no consistent performance for each host genotype. For plants both with and without space mutation, endophyte promoted host growth, regulated the rational distribution of ion content and nutrient elements in host plant. The space mutation had effects on plants performance as there was significant difference (P< 0.05) between mutation and without mutant for all of the tested indices except with plant height, aboveground biomass, CTK contents, underground Na+ and C/P, SK, Na, aboveground C.Conclusions The effects of host genotype on plant performance was much more pronounced and far greater than that of endophyte infection and mutation. Endophyte had effects on more indices of plants without mutant which suggest mutation may hide some effects of endophyte as its strong effects.

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