scholarly journals A model to study orienting responses in zebrafish, and applications towards the emotion-cognition interaction

2019 ◽  
Author(s):  
Bianca Gomes do Nascimento ◽  
Hingrid Suzzan Tarso Oliveira e Oliveira ◽  
Hadda Tercya Lima Silva ◽  
Diógenes Henrique de Siqueira-Silva ◽  
Monica Lima-Maximino ◽  
...  

AbstractOrienting responses (ORs) are whole-organism reflexes that are elicited by innocuous stimuli, and which decrease in magnitude after stimulus repetition. ORs represent relatively simple responses that can be used to study attentional processes, and are modulated by the organism’s state, including arousal and activation levels, as well as by emotional processes. Here we describe a simple method to study ORs in zebrafish, a model organism increasingly being used in behavioural neuroscience. After presentation of a static visual stimulus, an OR is elicited, characterized by approaching the stimulus and orienting towards it. After repeated stimulation, OR decreases, suggesting habituation. These responses are qualitatively altered by exposure to a fear-eliciting alarm substance (i.e., derived from the skin of a conspecific), since exposed animals avoid the visual stimulus and orient either away from the stimulus or towards it, but at a distance. The protocol can be used to study orienting responses, as well as the impact of fear and arousal on these reflexes.


2021 ◽  
Vol 13 (10) ◽  
pp. 1927
Author(s):  
Fuqin Li ◽  
David Jupp ◽  
Thomas Schroeder ◽  
Stephen Sagar ◽  
Joshua Sixsmith ◽  
...  

An atmospheric correction algorithm for medium-resolution satellite data over general water surfaces (open/coastal, estuarine and inland waters) has been assessed in Australian coastal waters. In situ measurements at four match-up sites were used with 21 Landsat 8 images acquired between 2014 and 2017. Three aerosol sources (AERONET, MODIS ocean aerosol and climatology) were used to test the impact of the selection of aerosol optical depth (AOD) and Ångström coefficient on the retrieved accuracy. The initial results showed that the satellite-derived water-leaving reflectance can have good agreement with the in situ measurements, provided that the sun glint is handled effectively. Although the AERONET aerosol data performed best, the contemporary satellite-derived aerosol information from MODIS or an aerosol climatology could also be as effective, and should be assessed with further in situ measurements. Two sun glint correction strategies were assessed for their ability to remove the glint bias. The most successful one used the average of two shortwave infrared (SWIR) bands to represent sun glint and subtracted it from each band. Using this sun glint correction method, the mean all-band error of the retrieved water-leaving reflectance at the Lucinda Jetty Coastal Observatory (LJCO) in north east Australia was close to 4% and unbiased over 14 acquisitions. A persistent bias in the other strategy was likely due to the sky radiance being non-uniform for the selected images. In regard to future options for an operational sun glint correction, the simple method may be sufficient for clear skies until a physically based method has been established.



2003 ◽  
Vol 47 (11) ◽  
pp. 85-92 ◽  
Author(s):  
E. Cotteux ◽  
P. Duchene

The bulking that occurs in biological wastewater treatment plants using activated sludge is very often controlled by the injection of sodium hypochlorite into the return activated sludge (RAS) stream. In the present study undertaken at two pilot plants fed with synthetic wastewater, the impact of the pass frequency of the sludge at the chlorine dosing point on the nitrifying flora is analysed. The pass frequency is one for the pilot plant 1 and two for the pilot plant 2. A dose of chlorine of 4.85 ± 0.05 g/kg/MLVSS per day was applied at both pilots. The preservative effect on nitrifying activity of the lowest concentration of chlorine at the dosing point and therefore of the highest pass frequency was evidenced. Among other tools, a simple method of measurement of the oxygen uptake rate enabled us to monitor the effect of chlorination on nitrification before recording an increase in the ammonia concentration in the bulking.



2018 ◽  
Vol 2018 ◽  
pp. 1-14
Author(s):  
José Carlos Ortiz-Bayliss ◽  
Ivan Amaya ◽  
Santiago Enrique Conant-Pablos ◽  
Hugo Terashima-Marín

When solving constraint satisfaction problems (CSPs), it is a common practice to rely on heuristics to decide which variable should be instantiated at each stage of the search. But, this ordering influences the search cost. Even so, and to the best of our knowledge, no earlier work has dealt with how first variable orderings affect the overall cost. In this paper, we explore the cost of finding high-quality orderings of variables within constraint satisfaction problems. We also study differences among the orderings produced by some commonly used heuristics and the way bad first decisions affect the search cost. One of the most important findings of this work confirms the paramount importance of first decisions. Another one is the evidence that many of the existing variable ordering heuristics fail to appropriately select the first variable to instantiate. Another one is the evidence that many of the existing variable ordering heuristics fail to appropriately select the first variable to instantiate. We propose a simple method to improve early decisions of heuristics. By using it, performance of heuristics increases.



Environments ◽  
2019 ◽  
Vol 6 (3) ◽  
pp. 27 ◽  
Author(s):  
Michel Bérengier ◽  
Judicaël Picaut ◽  
Bettina Pahl ◽  
Denis Duhamel ◽  
Benoit Gauvreau ◽  
...  

Traffic noise is considered by people as one of the most important sources of environmental discomfort. A way to limit the traffic noise is to reduce the noise emission, for example, by using specific low noise pavements, particularly in suburban areas. However, in real situations, it can be difficult to evaluate the impact of a given pavement, because it depends, for example, on the road geometry, the meteorological conditions, or the distance of the receiver position. Finally it can be difficult to select the most appropriate pavement for a given noise reduction objective. In this paper, a simple method is proposed to evaluate the noise impact of a pavement, in typical road geometries and environmental conditions. The proposed approach uses two databases, the first one based on measurements of emission spectra of road vehicles on several typical pavements, the second one made of pre-calculations of noise propagation for typical road configurations. Finally, the method is implemented in an interactive web tool, called DEUFRABASE, which allows one to obtain a fast estimation of the L Aeq (1 h or 24 h) and L den noise levels for various pavements and road configurations, as functions of the traffic flow and composition. By comparing the method with measurements, it is showed that the tool, although based on a restricted number of pavements and on several simplifications, can predict the noise impact of typical road configurations, with an acceptable error, most often less than 2 dB.



2021 ◽  
Author(s):  
Ge Cheng ◽  
David Grawe ◽  
K. Heinke Schlünzen

<p>Nudging is a simple method that aims to dynamically adjust the model toward the observations by including an additional feedback term in the model governing equation. This method is widely applied in data assimilation due to its simple implementation and reasonable model results. The basic concept of nudging is similar to that of urban canopy parameterization, in which additional terms are usually added in the conservation equations of momentum and energy aiming to simulate the canopy effects. However, few studies have investigated the implementation of nudging methods in urban canopy parameterizations. In this study we developed a multi-layer urban canopy parameterization (UCP) by using a nudging approach to represent the impacts of vegetated urban canopies on temperatures and winds in mesoscale models.</p><p>The difficulty of developing UCP by using a nudging method lies in defining appropriate values for the nudging coefficients and the forcing fields (e.g. indoor temperature fields for temperature nudging). To determine nudging coefficients, we use three major urban canopy morphological parameters: building height, frontal area density and building density. The ranges of these parameters are taken from the values for the Local Climate Zones datasets, in our case for the city of Hamburg. The UCP is employed in the three -dimensional atmospheric mesoscale model METRAS. Results show that this UCP can well simulate wind-blocking effects induced from obstacles as buildings and trees and urban heat island phenomenon for cities. Thus, nudging is an efficient and effective method that can be used for urban canopy parameterizations. However, as well known for nudging, it is not conserving energy. Therefore, we investigated the energy loss by tracking the reduced kinetic energy and internal energy. The UCP and model results will be presented.</p>



2021 ◽  
Vol 13 (23) ◽  
pp. 13085
Author(s):  
Jan Kowalski ◽  
Mieczysław Połoński ◽  
Marzena Lendo-Siwicka ◽  
Roman Trach ◽  
Grzegorz Wrzesiński

Exceeding the approved budget is often an integral part of the implementation of construction projects, especially those where unforeseen threats may occur. Therefore, each construction investment should contain elements of risk forecasting, mainly in terms of the cost of its implementation. Only a small number of institutions apply effective cost control methods, taking into account the specifics of a given industry. Especially small construction companies that participate in the structure of the implementation of large construction projects as subcontractors. The article presents a method by which it is possible to determine, with certain probability, the final cost of railway construction investments carried out in Poland. The method was based on a reliable database of risk factors published in sources. In this article, the main presumptions of the original method are presented, which take into account the impact of potential, previously recognized, risks specific to railway investments, and enable project managers to relate them to the conditions where the implementation of a specific object is planned. The authors assumed that such a relatively simple method, supported by a suitable computational program, would encourage teams that plan to implement railway projects to use it and increase the credibility of their schedules.



2020 ◽  
Author(s):  
Helena Rawsthorne ◽  
Fernando Calahorro ◽  
Emily Feist ◽  
Lindy Holden-Dye ◽  
Vincent O’Connor ◽  
...  

Abstract Autism spectrum disorder (ASD) is characterized by a triad of behavioural impairments including social behaviour. Neuroligin, a trans-synaptic adhesion molecule, has emerged as a penetrant genetic determinant of behavioural traits that signature the neuroatypical behaviours of autism. However, the function of neuroligin in social circuitry and the impact of genetic variation to this gene is not fully understood. Indeed, in animal studies designed to model autism, there remains controversy regarding the role of neuroligin dysfunction in the expression of disrupted social behaviours. The model organism, Caenorhabditis elegans, offers an informative experimental platform to investigate the impact of genetic variants on social behaviour. In a number of paradigms, it has been shown that inter-organismal communication by chemical cues regulates C. elegans social behaviour. We utilize this social behaviour to investigate the effect of autism-associated genetic variants within the social domain of the research domain criteria. We have identified neuroligin as an important regulator of social behaviour and segregate the importance of this gene to the recognition and/or processing of social cues. We also use CRISPR/Cas9 to edit an R-C mutation that mimics a highly penetrant human mutation associated with autism. C. elegans carrying this mutation phenocopy the behavioural dysfunction of a C. elegans neuroligin null mutant, thus confirming its significance in the regulation of animal social biology. This highlights that quantitative behaviour and precision genetic intervention can be used to manipulate discrete social circuits of the worm to provide further insight into complex social behaviour.



2019 ◽  
Vol 121 (6) ◽  
pp. 2202-2214 ◽  
Author(s):  
John P. McClure ◽  
Pierre-Olivier Polack

Multimodal sensory integration facilitates the generation of a unified and coherent perception of the environment. It is now well established that unimodal sensory perceptions, such as vision, are improved in multisensory contexts. Whereas multimodal integration is primarily performed by dedicated multisensory brain regions such as the association cortices or the superior colliculus, recent studies have shown that multisensory interactions also occur in primary sensory cortices. In particular, sounds were shown to modulate the responses of neurons located in layers 2/3 (L2/3) of the mouse primary visual cortex (V1). Yet, the net effect of sound modulation at the V1 population level remained unclear. In the present study, we performed two-photon calcium imaging in awake mice to compare the representation of the orientation and the direction of drifting gratings by V1 L2/3 neurons in unimodal (visual only) or multimodal (audiovisual) conditions. We found that sound modulation depended on the tuning properties (orientation and direction selectivity) and response amplitudes of V1 L2/3 neurons. Sounds potentiated the responses of neurons that were highly tuned to the cue’s orientation and direction but weakly active in the unimodal context, following the principle of inverse effectiveness of multimodal integration. Moreover, sound suppressed the responses of neurons untuned for the orientation and/or the direction of the visual cue. Altogether, sound modulation improved the representation of the orientation and direction of the visual stimulus in V1 L2/3. Namely, visual stimuli presented with auditory stimuli recruited a neuronal population better tuned to the visual stimulus orientation and direction than when presented alone. NEW & NOTEWORTHY The primary visual cortex (V1) receives direct inputs from the primary auditory cortex. Yet, the impact of sounds on visual processing in V1 remains controverted. We show that the modulation by pure tones of V1 visual responses depends on the orientation selectivity, direction selectivity, and response amplitudes of V1 neurons. Hence, audiovisual stimuli recruit a population of V1 neurons better tuned to the orientation and direction of the visual stimulus than unimodal visual stimuli.



Agronomy ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 120 ◽  
Author(s):  
Georg Röll ◽  
William Batchelor ◽  
Ana Castro ◽  
María Simón ◽  
Simone Graeff-Hönninger

Developing disease models to simulate and analyse yield losses for various pathogens is a challenge for the crop modelling community. In this study, we developed and tested a simple method to simulate septoria tritici blotch (STB) in the Cropsim-CERES Wheat model studying the impacts of damage on wheat (Triticum aestivum L.) yield. A model extension was developed by adding a pest damage module to the existing wheat model. The module simulates the impact of daily damage on photosynthesis and leaf area index. The approach was tested on a two-year dataset from Argentina with different wheat cultivars. The accuracy of the simulated yield and leaf area index (LAI) was improved to a great extent. The Root mean squared error (RMSE) values for yield (1144 kg ha−1) and LAI (1.19 m2 m−2) were reduced by half (499 kg ha−1) for yield and LAI (0.69 m2 m−2). In addition, a sensitivity analysis of different disease progress curves on leaf area index and yield was performed using a dataset from Germany. The sensitivity analysis demonstrated the ability of the model to reduce yield accurately in an exponential relationship with increasing infection levels (0–70%). The extended model is suitable for site specific simulations, coupled with for example, available remote sensing data on STB infection.



2019 ◽  
Vol 22 (11) ◽  
pp. 2490-2503 ◽  
Author(s):  
YT Zhang ◽  
B Shan ◽  
Y Xiao

Existing research on the widely used concrete-filled steel tubes is mainly focused on static or cyclic loading, and the studies on effects of high strain rate are relatively rare. In this article, seven stub concrete-filled steel tubular columns with square section were tested under both static and impact loads, using a large-capacity drop-weight testing machine. The research parameters were variable height of the drop-weight and different load types. The experimental results show that the failure modes of the concrete-filled steel tube columns from the impact tests are similar with those under static load, characterized by the local buckling of the steel tube. The time history curves of impact force and steel strain were investigated. The results indicate that with increasing impact energy, the concrete-filled steel tube stub columns had a stronger impact-resistant behavior. The dynamic analysis software LS-DYNA was employed to simulate the impact behaviors of the concrete-filled steel tube specimens, and the finite element results were reasonable compared with the test results. The parameter analysis on the impact behavior of concrete-filled steel tube columns was performed using the finite element model as well. A simple method was proposed to calculate the impact strength of square concrete-filled steel tube columns and compared favorably with experimental results.



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