scholarly journals A novel bacterial class V dye-decolourizing peroxidase from the extremophile Deinococcus radiodurans: cloning, expression optimization, purification, crystallization, initial characterization and X-ray diffraction analysis

2018 ◽  
Vol 74 (7) ◽  
pp. 419-424 ◽  
Author(s):  
Kelly Stefany Tuna Frade ◽  
Andreia Cecília Pimenta Fernandes ◽  
Celia Marisa Silveira ◽  
Carlos Frazão ◽  
Elin Moe
Keyword(s):  
Large Scale ◽  
Deinococcus Radiodurans ◽  
Resistance Mechanism ◽  
Ros Scavenging ◽  
Cell Parameters ◽  
Unit Structure ◽  
Crystallization Experiment ◽  
Initial Characterization ◽  
Protection Mechanisms ◽  
Different Temperatures

Deinococcus radiodurans is a bacterium with extreme resistance to desiccation and radiation. The resistance mechanism is unknown, but an efficient reactive oxygen species (ROS) scavenging system and DNA-repair and DNA-protection mechanisms are believed to play important roles. Here, the cloning and small- and medium-scale expression tests of a novel dye-decolourizing peroxidase from D. radiodurans (DrDyP) using three different Escherichia coli strains and three different temperatures in order to identify the optimum conditions for the expression of recombinant DrDyP are presented. The best expression conditions were used for large-scale expression and yielded ∼10 mg recombinant DrDyP per litre of culture after purification. Initial characterization experiments demonstrated unusual features with regard to the haem spin state, which motivated the crystallization experiment. The obtained crystals were used for data collection and diffracted to 2.2 Å resolution. The crystals belonged to the trigonal space group P31 or P32, with unit-cell parameters a = b = 64.13, c = 111.32 Å, and are predicted to contain one DrDyP molecule per asymmetric unit. Structure determination by molecular replacement using previously determined structures of dye-decolourizing peroxidases with ∼30% sequence identity at ∼2 Å resolution as templates are ongoing.

scholarly journals Allanite at high temperature: effect of REE on the thermal behaviour of epidote-group minerals

2021 ◽  
Vol 48 (9) ◽  
Author(s):  
G. Diego Gatta ◽  
Francesco Pagliaro ◽  
Paolo Lotti ◽  
Alessandro Guastoni ◽  
Laura Cañadillas-Delgado ◽  
...  
Keyword(s):  
Cell Volume ◽  
Thermal Behaviour ◽  
Unit Cell Volume ◽  
Unit Cell ◽  
Atomic Scale ◽  
Elastic Behaviour ◽  
Positive Trend ◽  
Cell Parameters ◽  
Thermal Anisotropy ◽  
Different Temperatures

AbstractThe thermal behaviour of a natural allanite-(Ce) has been investigated up to 1073 K (at room pressure) by means of in situ synchrotron powder X-ray diffraction and single-crystal neutron diffraction. Allanite preserves its crystallinity up to 1073 K. However, up to 700 K, the thermal behaviour along the three principal crystallographic axes, of the monoclinic β angle and of the unit-cell volume follow monotonically increasing trends, which are almost linear. At T > 700–800 K, a drastic change takes place: an inversion of the trend is observed along the a and b axes (more pronounced along b) and for the monoclinic β angle; in contrast, an anomalous increase of the expansion is observed along the c axis, which controls the positive trend experienced by the unit-cell volume at T > 700–800 K. Data collected back to room T, after the HT experiments, show unit-cell parameters significantly different with respect to those previously measured at 293 K: allanite responds with an ideal elastic behaviour up to 700 K, and at T > 700–800 K its behaviour deviates from the elasticity field. The thermo-elastic behaviour up to 700 K was modelled with a modified Holland–Powell EoS; for the unit-cell volume, we obtained the following parameters: VT0 = 467.33(6) Å3 and αT0(V) = 2.8(3) × 10–5 K−1. The thermal anisotropy, derived on the basis of the axial expansion along the three main crystallographic directions, is the following: αT0(a):αT0(b):αT0(c) = 1.08:1:1.36. The T-induced mechanisms, at the atomic scale, are described on the basis of the neutron structure refinements at different temperatures. Evidence of dehydroxylation effect at T ≥ 848 K are reported. A comparison between the thermal behaviour of allanite, epidote and clinozoisite is carried out.

Production, crystallization and preliminary X-ray analysis of the human integrin \alpha_1 I domain

1999 ◽  
Vol 55 (7) ◽  
pp. 1365-1367 ◽  
Author(s):  
Tiina A. Salminen ◽  
Yvonne Nymalm ◽  
Jussi Kankare ◽  
Jarmo Käpylä ◽  
Jyrki Heino ◽  
...  
Keyword(s):  
Large Scale ◽  
Cell Types ◽  
Diffusion Method ◽  
Scale Production ◽  
Data Set ◽  
Cell Parameters ◽  
Collagen Receptors ◽  
Large Scale Production ◽  
Peg 4000 ◽  
Reservoir Solution

Integrin α1β1 is one of the main collagen receptors in many cell types. A fast large-scale production, purification and crystallization method for the integrin α1 I domain is reported here. The α1 I domain was crystallized using the vapour-diffusion method with a reservoir solution containing a mixture of PEG 4000, sodium acetate, glycerol and Tris–HCl buffer. The crystals beong to the C2 space group, with unit-cell parameters a = 74.5, b = 81.9, c = 37.3 Å, α = γ = 90.0, β = 90.8°. The crystals diffract to 2.0 Å and a 94.2% complete data set to 2.2 Å has been collected from a single crystal with an R merge of 5.8%.

scholarly journals ELECTRICAL CONDUCTIVITY AND QUALITY OF DIFFERENT UMBEL ORDER CARROT SEEDS

2020 ◽  
Vol 9 (04) ◽  
pp. 75-81
Author(s):  
Luana de Souza Marinke ◽  
Hugo César Rodrigues Moreira Catão ◽  
Gabrielly Fernanda Francisco ◽  
Ítala Menegon Castilho ◽  
Letícia Rodrigues de Oliveira ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Germination Rate ◽  
Statistical Design ◽  
Germination Test ◽  
Aerial Portion ◽  
Initial Characterization ◽  
Different Temperatures ◽  
Physiological Quality ◽  
Final Score

Carrot seeds exhibit irregular vigor due to the wide blooming period of the crop. Results from germination tests are insufficient to evaluate the physiological potential. Thus, vigor tests are used in combination with germination tests. The objective of this work was to evaluate the quality of carrot seeds from different umbel order and the electrical conductivity under different temperatures and imbibing periods. Carrot seeds from cultivar Brasilia were used to perform the experiments. Content of water, the first and the final score of the germination test, index of germination rate, and length of roots and of the aerial portion were determined in the initial characterization. The electrical conductivity test was completed with replicates containing 50 seeds, in 25 mL deionized water at 20, 25 and 30oC. Evaluations were performed with 2, 4, 6, 8, 12 and 24 hours of imbibing period. The experiment was accomplished under a completely random statistical design. The quality of carrot seeds varied according to the umbel order. Seeds of secondary umbels showed better physiological quality. The electrical conductivity test is efficient to evaluate the vigor of carrot seeds with 8 hours of imbibing period at 25°C.

scholarly journals A putative siderophore-interacting protein from the marine bacteriumShewanella frigidimarinaNCIMB 400: cloning, expression, purification, crystallization and X-ray diffraction analysis

2016 ◽  
Vol 72 (9) ◽  
pp. 667-671 ◽  
Author(s):  
Inês B. Trindade ◽  
Bruno M. Fonseca ◽  
Pedro M. Matias ◽  
Ricardo O. Louro ◽  
Elin Moe
Keyword(s):  
Iron Acquisition ◽  
Crystallographic Analysis ◽  
Monoclinic Space Group ◽  
Molecular Replacement ◽  
X Ray Diffraction ◽  
Interacting Protein ◽  
X Ray ◽  
Cell Parameters ◽  
Unit Structure ◽  
Shewanella Frigidimarina

Siderophore-binding proteins (SIPs) perform a key role in iron acquisition in multiple organisms. In the genome of the marine bacteriumShewanella frigidimarinaNCIMB 400, the gene tagged as SFRI_RS12295 encodes a protein from this family. Here, the cloning, expression, purification and crystallization of this protein are reported, together with its preliminary X-ray crystallographic analysis to 1.35 Å resolution. The SIP crystals belonged to the monoclinic space groupP21, with unit-cell parametersa= 48.04,b= 78.31,c= 67.71 Å, α = 90, β = 99.94, γ = 90°, and are predicted to contain two molecules per asymmetric unit. Structure determination by molecular replacement and the use of previously determined ∼2 Å resolution SIP structures with ∼30% sequence identity as templates are ongoing.

scholarly journals Bulachite, [Al6(AsO4)3(OH)9(H2O)4]⋅2H2O from Cap Garonne, France: Crystal structure and formation from a higher hydrate

2020 ◽  
Vol 84 (4) ◽  
pp. 608-615
Author(s):  
Ian E. Grey ◽  
Emre Yoruk ◽  
Stéphanie Kodjikian ◽  
Holger Klein ◽  
Catherine Bougerol ◽  
...  
Keyword(s):  
Unit Cell ◽  
Orthorhombic Symmetry ◽  
X Ray Diffraction ◽  
Unit Cell Parameters ◽  
Space Group ◽  
X Ray ◽  
Cell Parameters ◽  
Different Temperatures ◽  
Using Data ◽  
Hydrated Aluminium

AbstractBulachite specimens from Cap Garonne, France, comprise two intimately mixed hydrated aluminium arsenate minerals with the same Al:As ratio of 2:1 and with different water contents. The crystal structures of both minerals have been solved using data from low-dose electron diffraction tomography combined with synchrotron powder X-ray diffraction. One of the minerals has the same powder X-ray diffraction pattern (PXRD) as for published bulachite. It has orthorhombic symmetry, space group Pnma with unit-cell parameters a = 15.3994(3), b = 17.6598(3), c = 7.8083(1) Å and Z = 4, with the formula [Al6(AsO4)3(OH)9(H2O)4]⋅2H2O. The second mineral is a higher hydrate with composition [Al6(AsO4)3(OH)9(H2O)4]⋅8H2O. It has the same Pnma space group and unit-cell parameters a = 19.855(4), b = 17.6933(11) and c = 7.7799(5) Å i.e. almost the same b and c parameters but a much larger a parameter. The structures are based on polyhedral layers, parallel to (100), of composition [Al6(AsO4)3(OH)9(H2O)4] and with H-bonded H2O between the layers. The layers contain [001] spiral chains of edge-shared octahedra, decorated with corner connected AsO4 tetrahedra that are the same as in the mineral liskeardite. The spiral chains are joined together by octahedral edge-sharing to form layers parallel to (100). Synchrotron PXRD patterns collected at different temperatures during heating of the specimen show that the higher-hydrate mineral starts transforming to bulachite when heated to 50°C, and the transformation is complete between 75 and 100°C.

scholarly journals Analysis of the impact of quick charge technology on the charging process parameters of the lithium-ion storage at various temperatures

2018 ◽  
Vol 19 ◽  
pp. 01035 ◽  
Author(s):  
Damian Burzyński ◽  
Damian Głuchy ◽  
Maksymilian Godek
Keyword(s):  
Process Parameters ◽  
Lithium Ion ◽  
Cell Parameters ◽  
Lithium Ion Cells ◽  
Lithium Ion Cell ◽  
Ion Storage ◽  
Different Temperatures ◽  
The Subject ◽  
The Impact

The paper deals with the subject of influence of the Quick Charge technology on the parameters of the charging process of lithium-ion cells. Tests of lithium-ion cell parameters during the charging process were performed at three different temperatures using conventional and accelerated charging. Also, the following paper comprises conclusions related to the conducted tests.

scholarly journals Purification, crystallization and preliminary crystallographic analysis of DR0248, an MNT–HEPN fused protein fromDeinococcus radiodurans

2015 ◽  
Vol 71 (1) ◽  
pp. 49-53
Author(s):  
Gaelle Pesce ◽  
Simone Pellegrino ◽  
Sean McSweeney ◽  
AnaMaria Goncalves ◽  
Daniele de Sanctis
Keyword(s):  
Deinococcus Radiodurans ◽  
Crystallographic Analysis ◽  
Unit Cell ◽  
Nucleotide Binding Domain ◽  
Unit Cell Parameters ◽  
Dodecyldimethylamine Oxide ◽  
Space Group ◽  
Cell Parameters ◽  
Nucleotidyl Transferase

DR0248 is a protein identified in theDeinococcus radiodurans(DR) genome that is predicted to encompass two domains: an N-terminal minimal nucleotidyl transferase domain (MNT) and a C-terminal higher eukaryotes and prokaryotes nucleotide-binding domain (HEPN). These two domains, usually encoded in two ORFs, have been suggested to play the role of a toxin–antitoxin (TA) system in prokaryotes. Recombinant DR0248 was overexpressed and purified fromEscherichia coliand diffraction-quality crystals were obtained in the presence of the detergent molecules dodecyldimethylamine oxide (DDAO) and octaethylene glycol monododecyl ether (C12E8), which were used as crystallization additives. Crystals grown with DDAO diffracted to a resolution of 2.24 Å and belonged to space groupC2221, with unit-cell parametersa= 98.4,b= 129.9,c= 59.2 Å. Crystals grown with C12E8 diffracted to a resolution of 1.83 Å and belonged to space groupP212121, with unit-cell parametersa= 51.6,b= 87.2,c= 108.2 Å. The structure was solved by multiwavelength anomalous dispersion from zinc bound to the protein using a single crystal obtained in the presence of DDAO.

scholarly journals Facile Synthesis, Microstructure, and Gas Sensing Properties of NdCoO3 Nanoparticles

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Lorenzo Gildo-Ortiz ◽  
Héctor Guillén-Bonilla ◽  
Juan Reyes-Gómez ◽  
Verónica María Rodríguez-Betancourtt ◽  
M. de la L. Olvera-Amador ◽  
...  
Keyword(s):  
Gas Sensing ◽  
Cobalt Nitrate ◽  
Diffusional Growth ◽  
Orthorhombic Crystal ◽  
Cell Parameters ◽  
Standard Atmosphere ◽  
Sensing Applications ◽  
Water As Solvent ◽  
Different Temperatures ◽  
Precursor Powders

NdCoO3 nanoparticles were successfully synthesized by a simple, inexpensive, and reproducible solution method for gas sensing applications. Cobalt nitrate, neodymium nitrate, and ethylenediamine were used as precursors and distilled water as solvent. The solvent was evaporated later by means of noncontinuous microwave radiation at 290 W. The obtained precursor powders were calcined at 200, 500, 600, and 700°C in a standard atmosphere. The oxide crystallized in an orthorhombic crystal system with space group Pnma (62) and cell parameters a=5.33 Å, b=7.52 Å, and c=5.34 Å. The nanoparticles showed a diffusional growth to form a network-like structure and porous adsorption configuration. Pellets prepared from NdCoO3 were tested as gas sensors in atmospheres of carbon monoxide and propane at different temperatures. The oxide nanoparticles were clearly sensitive to changes in gas concentrations (0–300 ppm). The sensitivity increased with increasing concentration of the gases and operating temperatures (25, 100, 200, and 300°C).

Preparation and Negative Thermal Expansion Property of ZrWMoO8

2010 ◽  
Vol 177 ◽  
pp. 245-248
Author(s):  
Juan Yang ◽  
Qin Qin Liu ◽  
Chuang Liang Zang ◽  
Xiao Nong Cheng
Keyword(s):  
Thermal Expansion ◽  
Negative Thermal Expansion ◽  
Mechanical Analysis ◽  
Cell Parameters ◽  
Thermal Expansion Property ◽  
In Situ Xrd ◽  
Structure And Morphology ◽  
Expansion Property ◽  
Different Temperatures

Negative thermal expansion (NTE) material ZrWMoO8 was prepared by hydrothermal method. The phase structure and morphology of the obtained precursor and final product were examined by XRD and SEM, respectively. To study its negative thermal expansion property, two methods were utilized. One is based on the in-situ XRD results and the thermal expansion coefficient (CTE) was calculated by the cell parameters obtained at different temperatures. Another is based on the thermo-mechanical analysis (TMA) and the CTE was calculated by the length of ZrWMoO8 bar at different temperatures. The differences between these two methods were also discussed.

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