Multi-analyte detection handheld analyzer for point-of-care application with disposable biochips

2004 ◽  
Author(s):  
M. Dutta ◽  
S. Chilukuru ◽  
L. Ramasamy ◽  
Xiaoshan Zhu ◽  
Jaephil Do ◽  
...  
Keyword(s):  
Point Of Care ◽  
Analyte Detection

scholarly journals Lab-on-a-Chip Systems for Aptamer-Based Biosensing

Micromachines ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 220 ◽  
Author(s):  
Niazul I. Khan ◽  
Edward Song
Keyword(s):  
Point Of Care ◽  
Lab On A Chip ◽  
Review Article ◽  
Microfluidic Chips ◽  
High Affinity ◽  
Sensing Platforms ◽  
Personal Medicine ◽  
Paper Based Microfluidics ◽  
Recognition Elements ◽  
Analyte Detection

Aptamers are oligonucleotides or peptides that are selected from a pool of random sequences that exhibit high affinity toward a specific biomolecular species of interest. Therefore, they are ideal for use as recognition elements and ligands for binding to the target. In recent years, aptamers have gained a great deal of attention in the field of biosensing as the next-generation target receptors that could potentially replace the functions of antibodies. Consequently, it is increasingly becoming popular to integrate aptamers into a variety of sensing platforms to enhance specificity and selectivity in analyte detection. Simultaneously, as the fields of lab-on-a-chip (LOC) technology, point-of-care (POC) diagnostics, and personal medicine become topics of great interest, integration of such aptamer-based sensors with LOC devices are showing promising results as evidenced by the recent growth of literature in this area. The focus of this review article is to highlight the recent progress in aptamer-based biosensor development with emphasis on the integration between aptamers and the various forms of LOC devices including microfluidic chips and paper-based microfluidics. As aptamers are extremely versatile in terms of their utilization in different detection principles, a broad range of techniques are covered including electrochemical, optical, colorimetric, and gravimetric sensing as well as surface acoustics waves and transistor-based detection.

An inkjet-printed smartphone-supported electrochemical biosensor system for reagentless point-of-care analyte detection

2021 ◽  
pp. 130447
Author(s):  
Yang Bai ◽  
Qiuquan Guo ◽  
Junfeng Xiao ◽  
Mingyue Zheng ◽  
Dongxing Zhang ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Point Of Care ◽  
Biosensor System ◽  
Analyte Detection

scholarly journals The use of nanobodies in a sensitive ELISA test for SARS-CoV-2 Spike 1 protein

2021 ◽  
Vol 8 (9) ◽  
Author(s):  
Georgina C. Girt ◽  
Abirami Lakshminarayanan ◽  
Jiandong Huo ◽  
Joshua Dormon ◽  
Chelsea Norman ◽  
...  
Keyword(s):  
Point Of Care ◽  
Sandwich Elisa ◽  
Elisa Test ◽  
Spike Protein ◽  
Receptor Binding Domain ◽  
Established Method ◽  
Compact Structure ◽  
Antigen Capture ◽  
Analyte Detection ◽  
Secondary Ligand

Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens in the fluid has important uses in biotechnology, and is integral to many point-of-care SARS-CoV-2 diagnostics. Sandwich enzyme-linked immunosorbent assays (ELISAs) are a sensitive, well-established method of measuring antigens in solutions. They use one ligand to capture and the other ligand to detect the target analyte. Detection is commonly achieved using colorimetric readout obtained upon the reaction of a substrate with HRP-conjugated secondary ligand. Nanobodies, the V H H domain of camelid antibodies, have expanded the repertoire of molecules used in antigen detection. Nanobodies' high affinity for target antigens, their compact structure, their high stability and ease of production has driven research into their use as diagnostic reagents. Guided by a structural understanding of epitopes on the receptor-binding domain of the SARS-CoV-2 Spike protein, we investigated various combinations of engineered nanobodies in a sandwich ELISA to detect the Spike protein of SARS-CoV-2. We have identified an optimal combination of nanobodies. These were selectively functionalized to further improve antigen capture, enabling the measurement of sub-picomolar amounts of SARS-CoV-2 Spike protein in solution. With this combination, the routine detection limit in samples inactivated by heat and detergent corresponded to less than seven focus-forming units of infectious SARS-CoV-2.

scholarly journals Towards an Integrated System as Point-of-Care Device for the Optical Detection of Sepsis Biomarkers

Chemosensors ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 12 ◽  
Author(s):  
Ambra Giannetti ◽  
Cosimo Trono ◽  
Giampiero Porro ◽  
Claudio Domenici ◽  
Mariarita Puntoni ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Severe Infection ◽  
Integrated System ◽  
Serum Samples ◽  
Delay In Diagnosis ◽  
Sepsis Diagnosis ◽  
Reactive Protein ◽  
Inflammatory Processes ◽  
Analyte Detection

Severe infection and sepsis are a common, expensive, and frequently fatal conditions in critically ill patients. The sepsis diagnosis is not trivial, since it is an extremely complex chain of events involving inflammatory and anti-inflammatory processes, cellular reactions, and circulatory disorders. For these reasons, delay in diagnosis and initiation of drug treatments have shown to be crucial for this pathology. Moreover, a multitude of biomarkers has been proposed, many more than for other pathologies. In order to select optimal treatments for the highly heterogeneous group of sepsis patients and to reduce costs, novel multiplexed tools that better characterize the patient and his or her specific immune response are highly desired. In order to achieve the fundament of drastically improved multi-analyte detection and to attain low limits of detection in diagnostics, the area of point-of-care testing (POCT) technology is developing quickly, leading to the production of instruments, the reliability of which is continuously increasing. For this purpose, a selection of two biomarkers—C-reactive protein (CRP) and neopterin (NP)—was studied in this paper and a fluorescence-based integrated optical system, suitable for future POCT applications, was implemented that is capable of performing the simultaneous measurement of the two different biomarkers in replicate. A limit of detection of 10 and 2.1 µg L−1 was achieved for CRP and NP spiked in commercially available human serum, respectively. Moreover, measurements on both biomarkers were also performed on serum samples collected from septic patients.

scholarly journals Lab-on-chip systems for integrated bioanalyses

2016 ◽  
Vol 60 (1) ◽  
pp. 121-131 ◽  
Author(s):  
João Pedro Conde ◽  
Narayanan Madaboosi ◽  
Ruben R.G. Soares ◽  
João Tiago S. Fernandes ◽  
Pedro Novo ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Point Of Care ◽  
Qualitative Assessment ◽  
Biomolecular Detection ◽  
Seamless Integration ◽  
Lab On Chip ◽  
Point Of Use ◽  
Analyte Detection ◽  
On Chip ◽  
The Right

Biomolecular detection systems based on microfluidics are often called lab-on-chip systems. To fully benefit from the miniaturization resulting from microfluidics, one aims to develop ‘from sample-to-answer’ analytical systems, in which the input is a raw or minimally processed biological, food/feed or environmental sample and the output is a quantitative or qualitative assessment of one or more analytes of interest. In general, such systems will require the integration of several steps or operations to perform their function. This review will discuss these stages of operation, including fluidic handling, which assures that the desired fluid arrives at a specific location at the right time and under the appropriate flow conditions; molecular recognition, which allows the capture of specific analytes at precise locations on the chip; transduction of the molecular recognition event into a measurable signal; sample preparation upstream from analyte capture; and signal amplification procedures to increase sensitivity. Seamless integration of the different stages is required to achieve a point-of-care/point-of-use lab-on-chip device that allows analyte detection at the relevant sensitivity ranges, with a competitive analysis time and cost.

“Aspirin - resistance”? A few critical considerations on definition, terminology, diagnosis, clinical value, natural course of atherosclerotic disease, and therapeutic consequences

VASA ◽  
2011 ◽  
Vol 40 (6) ◽  
pp. 429-438 ◽  
Author(s):  
Berent ◽  
Sinzinger
Keyword(s):  
Platelet Function ◽  
Point Of Care ◽  
Aspirin Resistance ◽  
Point Of View ◽  
Evidence Based ◽  
Platelet Function Tests ◽  
Clinical Value ◽  
Vascular Events ◽  
Therapeutic Consequences ◽  
Function Tests

Based upon various platelet function tests and the fact that patients experience vascular events despite taking acetylsalicylic acid (ASA or aspirin), it has been suggested that patients may become resistant to the action of this pharmacological compound. However, the term “aspirin resistance” was created almost two decades ago but is still not defined. Platelet function tests are not standardized, providing conflicting information and cut-off values are arbitrarily set. Intertest comparison reveals low agreement. Even point of care tests have been introduced before appropriate validation. Inflammation may activate platelets, co-medication(s) may interfere significantly with aspirin action on platelets. Platelet function and Cox-inhibition are only some of the effects of aspirin on haemostatic regulation. One single test is not reliable to identify an altered response. Therefore, it may be more appropriate to speak about “treatment failure” to aspirin therapy than using the term “aspirin resistance”. There is no evidence based justification from either the laboratory or the clinical point of view for platelet function testing in patients taking aspirin as well as from an economic standpoint. Until evidence based data from controlled studies will be available the term “aspirin resistance” should not be further used. A more robust monitoring of factors resulting in cardiovascular events such as inflammation is recommended.

Cloud-Computing: eHealth aus der Wolke

2018 ◽  
Vol 23 (11) ◽  
pp. 38-41
Author(s):  
Sebastian Krolop ◽  
Florian Benthin ◽  
Constanze Knahl
Keyword(s):  
Cloud Computing ◽  
Point Of Care

Cloud-Computing gewinnt auch in Kliniken zunehmend an Bedeutung. Über das Internet bereitgestellte Lösungen verändern nicht nur Verwaltung und Logistik – im klinischen Bereich geht es zum Beispiel um die Nutzung elektronischer Patientenakten am Point-of-Care.

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