Semi-Automated and Manual Methods for Counting Cells Expressing P75 Receptor in Endometriotic Lesions

A previously described comprehensive respiratory information system (CRIS) has been changed to introduce new spirometric tests which are sensitive to minor abnormalities, revise on the basis of additional data the regression equations which define normal values to various parameters of pulmonary function and refine the system’s interpretation scheme. The beneficial effects of transferring the system from a large IBM 360/65 to a small CDC 1700 are presented. An analysis of the costs of processing routine pulmonary function studies reveals that a 40°/o saving is realized when a computer is used in comparison to the use of the usual manual methods.

Download Full-text

Reballing/Rebumping Ultra-Fine Pitch Packages Less Than 0.5 mm Pitch for FA

ISTFA 2014: Conference Proceedings from the 40th International Symposium for Testing and Failure Analysis ◽

10.31399/asm.cp.istfa2014p0160 ◽

2014 ◽

Author(s):

Bob Wettermann

Keyword(s):

Solder Joint ◽

Failure Analysis ◽

Joint Strength ◽

Semiconductor Devices ◽

X Ray ◽

Fine Pitch ◽

Manual Methods

Abstract As the pitch and package sizes of semiconductor devices have shrunk and their complexity has increased, the manual methods by which the packages can be re-bumped or reballed for failure analysis have not kept up with this miniaturization. There are some changes in the types of reballing preforms used in these manual methods along with solder excavation techniques required for packages with pitches as fine as 0.3mm. This paper will describe the shortcomings of the previous methods, explain the newer methods and materials and demonstrate their robustness through yield, mechanical solder joint strength and x-ray analysis.

Download Full-text

Faculty Opinions recommendation of A rapid switch in sympathetic neurotransmitter release properties mediated by the p75 receptor.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1007152.89505 ◽

2002 ◽

Author(s):

Yves-Alain Barde

Keyword(s):

Neurotransmitter Release ◽

P75 Receptor

Download Full-text

Impact Analysis of the Mechanization Program for Tribal Paddy Farmers of Goa

Journal of AgriSearch ◽

10.21921/jas.v6i1.14922 ◽

2019 ◽

Vol 6 (1) ◽

Author(s):

MATHALA JULIET GUPTA ◽

ASHISH M. PITRE ◽

SUMATI CHAVAN PANDURNAG ◽

SALONI SALIL VANJARI

Keyword(s):

Impact Analysis ◽

Field Capacity ◽

Significant Saving ◽

Manual Methods ◽

Paddy Farmers ◽

The Impact ◽

And Training

This paper assessed the impact of the mechanization of the 8 tribal paddy farmers’ groups of Goa benefited in the year 2011 through the Tribal sub-plan program of ICAR-CCARI through results of surveys conducted in 2012 and 2015. Shift to mechanization among beneficiaries was significant in power tillers (64-100%) but less in power reapers(0-91%). Also significant saving in manpower (Power tillers:33.3% to 60%, power reapers: 33.3% to 83.3%), , time (field capacity increased (power tillers : 41.7% to141%, power reapers :58.1% to 912.8%) and cost(power tillers :44.7% to 59.1%, power reapers : 57.8% to 82.9%) was reportedthrough the use of equipment as compared to desi plough or manual methods of harvesting. Some constraints like lack of access roads and training in use and maintenance of the equipment were reported by the beneficiary farmers.

Download Full-text

Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma

Animals ◽

10.3390/ani11061478 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1478

Author(s):

Lorenzo G. T. M. Segabinazzi ◽

Giorgia Podico ◽

Michael F. Rosser ◽

Som G. Nanjappa ◽

Marco A. Alvarenga ◽

...

Keyword(s):

Blood Transfusion ◽

Platelet Rich Plasma ◽

Upright Position ◽

Platelet Counts ◽

Cell Counts ◽

Blood Cell Counts ◽

Platelet Concentration ◽

Manual Methods ◽

White Blood Cell Counts ◽

Over Time

In light of PRP’s increasing popularity in veterinary practice, this study aimed to compare three manual methods to prepare and cool equine PRP. The blood of 18 clinically healthy mares was collected via venipuncture in a blood transfusion bag (method 1), blood tubes (method 2), and a syringe (method 3). In method 1, samples were double centrifuged; method 2 involved one centrifugation, and in method 3 the syringe was kept in an upright position to sediment for 4 h. After processing with three methods, PRP and platelet-poor plasma (PPP) were extracted and assessed for red (RBC) and white blood cell counts (WBC), platelet counts, and viability. In a subset of mares (n = 6), samples were processed with the three methods, and PRP was evaluated at 6 and 24 h postcooling at 5 °C. Method 1 resulted in the highest and method 3 in the lowest platelet concentration (p < 0.05), and the latter also had greater contamination with WBC than the others (p < 0.001). Platelet viability was similar across treatments (p > 0.05). Cooling for 24 h did not affect platelet counts in all methods (p > 0.05); however, platelet viability was reduced after cooling PRP produced by method 3 (p = 0.04), and agglutination increased over time in all methods (p < 0.001). The three methods increased (1.8–5.6-fold) platelet concentration in PRP compared to whole blood without compromising platelet viability. In conclusion, all three methods concentrated platelets and while cooling affected their viability. It remains unknown whether the different methods and cooling would affect PRP’s clinical efficacy.

Download Full-text

Semi-automated cerebral aneurysm segmentation and geometric analysis for WEB sizing utilizing a cloud-based computational platform

Interventional Neuroradiology ◽

10.1177/15910199211009111 ◽

2021 ◽

pp. 159101992110091

Author(s):

Ansaar T Rai ◽

Ryan G Brotman ◽

Gerald R Hobbs ◽

SoHyun Boo

Keyword(s):

Geometric Analysis ◽

Good Reliability ◽

Moderate Agreement ◽

Manual Methods ◽

Web Device ◽

Computational Platform ◽

Neck Diameter ◽

Aneurysm Diameter ◽

Intra Class Correlation Coefficient ◽

The Web

Background Accurate aneurysm measurements are important for selecting the WEB device. The objective was to validate a cloud-based platform, SurgicalPreview (SP) against manual measurements for aneurysm analysis. Methods Two sets of measurements each for SP and manual methods were obtained for 40 aneurysms. Reliability and agreement were assessed with intra-class correlation coefficient (ICC) and Bland-Altman plots respectively. Kappa coefficient was used to assess agreement for predicting WEB size. Results There was good reliability for repeat SP measurements: aneurysm diameter (ICC-1, 95%CI 0.98–1), height (ICC-1, 95%CI 0.99–1) and neck diameter (ICC-0.96, 95%CI 0.93–0.98). There was good reliability for the two manual diameter (ICC-0.97, 95%CI 0.9–0.97) and height (ICC-0.93, 95%CI 0.87–0.96) measurements and moderate for neck diameter (ICC-0.76, 95%CI 0.54–0.87). There was greater agreement for SP versus manual repeat measurements on Bland-Altman plots. Reliability between the SP and manual methods was good for aneurysm diameter (ICC-0.98, 95%CI 0.95–1) and height (ICC-0.96, 95%CI-0.93–0.98) and moderate for neck. (ICC-0.6, 95%CI -0.22–0.87). The Bland-Altman plots confirmed better agreement between the two methods for the aneurysm diameter and height than the neck. There was strong agreement between the methods for predicting the WEB diameter (Kappa-0.84, 95%CI 0.71–0.97) and moderate for predicting WEB height (Kappa-0.66, 95%CI 0.43–0.89). There was moderate agreement for predicted versus deployed WEB diameter: SP (Kappa-0.56, 95%CI 0.38–0.74), Manual (Kappa-0.53, 95%CI 0.34–0.71). Conclusion The SurgicalPreview® had greater agreement for repeat measurements. There was good reliability between the two methods for predicting WEB diameter and height and moderate agreement between predicted versus deployed WEB diameter.

Download Full-text

Critical Aspects Concerning the Development of a Pooling Approach for SARS-CoV-2 Diagnosis Using Large-Scale PCR Testing

Viruses ◽

10.3390/v13050902 ◽

2021 ◽

Vol 13 (5) ◽

pp. 902

Author(s):

Daniel Cruceriu ◽

Oana Baldasici ◽

Loredana Balacescu ◽

Stefana Gligor-Popa ◽

Mirela Flonta ◽

...

Keyword(s):

Large Scale ◽

Rna Extraction ◽

Diagnostic Assay ◽

Assay Sensitivity ◽

Infected People ◽

Multiple Parameters ◽

Accurate Procedure ◽

Manual Methods ◽

Pooling Strategy ◽

Critical Aspects

The primary approach to controlling the spread of the pandemic SARS-CoV-2 is to diagnose and isolate the infected people quickly. Our paper aimed to investigate the efficiency and the reliability of a hierarchical pooling approach for large-scale PCR testing for SARS-CoV-2 diagnosis. To identify the best conditions for the pooling approach for SARS-CoV-2 diagnosis by RT-qPCR, we investigated four manual methods for both RNA extraction and PCR assessment targeting one or more of the RdRp, N, S, and ORF1a genes, by using two PCR devices and an automated flux for SARS-CoV-2 detection. We determined the most efficient and accurate diagnostic assay, taking into account multiple parameters. The optimal pool size calculation included the prevalence of SARS-CoV-2, the assay sensitivity of 95%, an assay specificity of 100%, and a range of pool sizes of 5 to 15 samples. Our investigation revealed that the most efficient and accurate procedure for detecting the SARS-CoV-2 has a detection limit of 2.5 copies/PCR reaction. This pooling approach proved to be efficient and accurate in detecting SARS-CoV-2 for all samples with individual quantification cycle (Cq) values lower than 35, accounting for more than 94% of all positive specimens. Our data could serve as a comprehensive practical guide for SARS-CoV-2 diagnostic centers planning to address such a pooling strategy.

Download Full-text