Comparative ejaculatory response, fresh and frozen semen quality and fertility to artificial vagina vs electroejaculation method of semen collection in mithun ( Bos frontalis ) bulls

Andrologia ◽  
2021 ◽  
Author(s):  
Saddamhusen Mabusab Nadaf ◽  
Vikram Ramesh ◽  
Moonmoon Mech ◽  
Meraj Haider Khan ◽  
Fazal Ali Ahmed ◽  
...  
Keyword(s):  
Semen Quality ◽  
Semen Collection ◽  
Frozen Semen ◽  
Artificial Vagina

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

104 COMPARING DIFFERENT LEVELS OF OSMOLALITY OF SUCROSE EXTENDER ON THE VIABILITY OF SPERMATOZOA IN BACTRIAN CAMEL (CAMELUS BACTRIANUS)

2006 ◽  
Vol 18 (2) ◽  
pp. 160 ◽  
Author(s):  
S. Mosaferi ◽  
A. Niasari-Naslaji ◽  
N. Bahmani ◽  
A. A. Gharahdaghi ◽  
A. Abarghani ◽  
...  
Keyword(s):  
Semen Quality ◽  
Egg Yolk ◽  
Bactrian Camel ◽  
Dromedary Camel ◽  
Camelus Bactrianus ◽  
Semen Collection ◽  
History Of ◽  
Different Levels ◽  
Artificial Vagina

Disaccharides have been used as an extender for dromedary camel semen (Bravo et al. 2000 Anim. Reprod. Sci. 62, 173-193). More recently we have investigated the effect of different concentrations of lactose extender on the viability of Bactrian camel spermatozoa (Mosafer et al. 2005 Reprod. Fertil. Dev. 17, 197). Considering the osmolality (316.1 � 1.48 mOsm/kg) and pH (7.4 � 0.03) of Bactrian camel semen (Mosaferi et al. 2005 Theriogenology 63, 92-101), the objective of this study was to investigate the effect of osmolality of sucrose extender on the viability of Bactrian camel spermatozoa. Sucrose at the concentrations of 9, 10, 11, 12, and 13% with osmolalities of 292, 331, 356, 386, and 410 mOsm/kg, respectively, were prepared. All extenders contained 20% egg yolk and antibiotics, with pH adjusted to 6.9. Semen was collected from camels with a sound history of semen quality and fertility (n = 3) using a modified artificial vagina and divided into different treatments after mechanical reduction of semen viscosity (3). Progressive forward motility of spermatozoa was examined at the time of semen collection and at 4, 12, and 24 h after incubation at 4�C. Data were analyzed using the GLM procedure in SAS/STAT after arcsin transformation. At the time of semen dilution, the progressive forward motility of spermatozoa was greater at osmolality of 331 (23%) compared with 292 (1%), 386 (6%), and 410 (3.5%) mOsm/kg (P < 0.05). No progressive forward motility of spermatozoa was noticed after 4 h incubation at 4�C at osmolalities of 292, 386, and 410 mOsm/kg. At this time, a significant decrease (P < 0.05) of progressive forward motility occurred at osmolalities of 331 (4%) and 356 (0.5%) compared with that of the time of dilution. After 12 and 24 h incubation at 4�C, no progressive forward motility of spermatozoa was detected in any of these extenders. In conclusion, 10% sucrose (331 mOsm/kg) at the adjusted pH of 6.9 was the most suitable concentration of this disaccharide for preserving Bactrian camel semen for less than 4 h under chilled conditions.

scholarly journals Karakteristik Semen dan Komposisi Kimia Plasma Seminalis Sapi Aceh yang Dipelihara di BIBD Saree Aceh Besar

2016 ◽  
Vol 16 (2) ◽  
pp. 121
Author(s):  
Zulyazaini Zulyazaini ◽  
Dasrul Dasrul ◽  
Sri Wahyuni ◽  
Muslim Akmal ◽  
Mohd. Agus Nashri Abdullah
Keyword(s):  
Chemical Composition ◽  
Seminal Plasma ◽  
Total Protein ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Plasma Chemical ◽  
Frozen Semen ◽  
Semen Characteristics ◽  
Fresh Semen ◽  
Artificial Vagina

ABSTRAK. Penelitian ini bertujuan untuk menentukan karakteristik dan komposisi plasma semen sapi aceh. Sampel semen dikoleksi dari 2 ekor sapi aceh jantan sehat berumur 3,0-3,5 tahun menggunakan vagina buatan. Koleksi semen dilakukan satu kali dalam seminggu selama enam minggu. Evaluasi karakteristik semen meliputi volume, warna, konsistensi, pH, konsentrasi spermatozoa, motilitas, viabilitas, abnormalitas spermatozoa dan komposisi kimia plasma semen. Data kualitas dan komposisi kimia plasma semen yang diperoleh dianalisa secara diskriptif. Hasil penelitian menunjukkan semen segar sapi aceh memiliki volume 3,82 ± 0,47 ml, warna krem keputihan, konsistensi kental, pH 6,84 ± 0,17, konsentrasi 1194 ± 4,80 × 106sp/ml, persentase motilitas 77,28 ± 3,17 % dan abnormalitas 5,98 ± 1,77%. Plasma seminalis semen segar sapi aceh mengandung protein total sebanyak 1,51 ± 0,21 g/100ml, Kalium 75,80 ± 22,63 mg/100 ml dan Magnesium 32,30 ± 17,64 mg/100 ml lebih tinggi, tetapi Natrium 170,00 ± 81,60 mg/100 ml; kalsium 40,00 ± 12,76 mg/100 ml, Fosfor 16,70 ± 6,17 mg/100 ml, dan Mangan 7,08 ± 2,48 mg/100 ml lebih rendah dibandingkan dengan sapi potong. Secara umum kuantitas dan komposisi plasma seminalis semen sapi aceh yang dihasilkan cukup baik, dan memenuhi syarat untuk diproses sebagai semen beku. (The characteristics of semen and chemical composition of the seminal plasma of aceh cattle maintaned in BIBD Saree Aceh Besar) ABSTRACT. This study aim were to determine characteristics and composition of semen plasma Aceh cattle. Semen samples were collected from two healthy Aceh bulls that aged from 3.0 to 3.5 year. Samples were yielded by using an artificial vagina. Samples collecting were conducted once a week for six consecutive weeks. The evaluation of semen characteristics includes volume, color, consistency, pH, sperm concentration, motility, viability, abnormal spermatozoa and seminal plasma chemical composition. Data of semen quality and chemical composition of seminal plasma were analyzed descriptively. The evaluation showed that Aceh Cattle semen had 3.82 ± 0.47 ml volume, beige whitish color, thick consistency, 6.84 ± 0.17 pH, 1194 ± 4.80×106 sp/ml sperm concentration, 77.28 ± 3.17% motility and 5.98 ± 1.77% abnormal spermatozoa. Plasma seminalis fresh semen of Aceh Cattle contained 1,51 ± 0,21 g/100ml total protein; 75,80 ± 22,63 mg/100ml potassium and 32,30 ± 17,64 mg/100ml magnesium higher than the heifer, but 170,00 ± 81,60 mg/100ml sodium; 40,00 ± 12,76 mg/100ml calcium, 16,70 ± 6,17 mg/100ml fosfor, and 7,08 ± 2,48 mg/100ml mangan lower. It was concluded that quality and composition of seminal plasma Aceh cattle semen could be made as frozen semen.

scholarly journals 94 COMPARING DIFFERENT LEVELS OF OSMOLARITY AND pH OF LACTOSE EXTENDER ON THE VIABILITY OF SPERMATOZOA IN THE BACTRIAN CAMEL (CAMELUS BACTRIANUS)

2005 ◽  
Vol 17 (2) ◽  
pp. 197 ◽  
Author(s):  
S. Mosaferi ◽  
A. Niasari-Naslaji ◽  
A.A. Gharahdaghi ◽  
A. Abarghani ◽  
A. Ghanbari ◽  
...  
Keyword(s):  
Semen Quality ◽  
Egg Yolk ◽  
Bactrian Camel ◽  
Mechanical Removal ◽  
Semen Collection ◽  
Arcsine Transformation ◽  
History Of ◽  
Semen Extender ◽  
Different Levels ◽  
Artificial Vagina

Lactose has been used widely as a semen extender for camels although in the absence of evidence illustrating its suitablility. Considering the osmolarity (316.1 ± 1.48 mOsm/kg) and pH (7.4 ± 0.03) of Bactrian camel semen (Mosaferi S et al. 2004 Theriogenology, in press), the objective of this study was to investigate the effect of osmolarity and pH of lactose extender on the viability of Bactrian camel spermatozoa. In Experiment I, with pH adjusted to 6.9, the effect of lactose concentrations of 9, 10, 11, 12, and 13% with an osmolarity of 290, 333, 350, 376, and 419 mOsm/kg, respectively, on the viability of spermatozoa was investigated. In Experiment II, with lactose fixed at 10%, the effect of extender with pH of 5.9, 6.9, 7.5, 7.9, and 8.9 on the viability of spermatozoa was examined. All extenders contained 20% egg yolk. In both experiments, semen was collected from camels with a sound history of semen quality and fertility (n = 3), using a modified artificial vagina, and divided into different treatments after mechanical removal of semen viscosity (Mosaferi et al. 2004). Progressive forward motility of spermatozoa was examined at the time of semen collection and at 4, 12, and 24 h after incubation at 4°C. Data were analyzed using the GLM procedure in SAS/STAT (SAS Institute, Inc., Cary, NC, USA) after arcsine transformation. At the time of semen dilution, the progressive forward motility of spermatozoa was greater at osmolarity of 290 (28.5%), 333 (34%), and 350 (31%) compared to 376 (13.5%) and 419 (1%) mOsm/kg (P < 0.05). The same trend in the progressive forward motility of spermatozoa was noticed after 4 h of incubation at 4°C; although a significant decrease (P < 0.05) occurred at 290 (11%), 333 (18 %) and 350 (16%). After 12 and 24 h of incubation at 4°C, the progressive forward motility of spermatozoa was less than 10% at 333 and 350 mOsm/kg (P < 0.05). At the time of semen dilution, the progressive forward motility of spermatozoa was greater (P < 0.05) at pH 6.9 (35.5%) and 7.5 (18%) compared to pH 5.9 (0%), 7.9 (7.5%) and 8.9 (2.5%). The same trend in the progressive forward motility of spermatozoa was observed after 4 h incubation at 4°C; although, a significant decrease (P < 0.05) occurred at pH 6.9 (15%) and 7.5 (9%) at this time. After 12 h incubation at 4°C, the progressive forward motility of spermatozoa was less than 5% at pH 6.9 and 7.5 (P < 0.05). In conclusion, 10% (333 mOsm/kg) and 11% (350 mOsm/kg) lactose, at the adjusted pH of 6.9, were the most suitable concentrations of lactose extender for preserving Bactrian camel semen for less than 4 h after which the viability of spermatozoa deteriorated significantly in this extender. The authors wish to thank the director and station staff of Bactrian Camel Research Center at Jahadabad, Meshkinshahr, Ardabil, for kind provision of facilities and assistance throughout the experiment.

scholarly journals Increased Integrity of Plasma Membrane and Acrosome Cap Spermatozoa Limousin Cattle at Post Thawing in Frozen Media by adding Seawater Extract

2017 ◽  
Vol 3 (6) ◽  
pp. 633
Author(s):  
Nur Faidah ◽  
Tatik Hernawati ◽  
Mirni Lamid ◽  
Ismudiono Ismudiono ◽  
Tri Wahyu Suprayogi ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Experimental Design ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Skim Milk ◽  
Egg Yolk ◽  
Duncan Multiple Range Test ◽  
Frozen Semen ◽  
Artificial Vagina ◽  
Range Test

This research was determined membrane integrity and acrosome cap of Limousin bull post thawing after adding seawater extract with different concentrations in extender skim milk and egg yolk to increased frozen semen quality. This research used fresh samples of Limousin bull’s semen collected by using artificial vagina, then devided into 4 treatments and 6 replications. The experimental design that used was Complete Random Design. Analysis of the data using Analysis of Variant (ANOVA) one way then proceeds to the Duncan Multiple Range Test to determine significant differences between treatments.The first treatment P0 no seawater extract added as a control. The second treatment P1 was treated with 0.109 µL seawater extract, P2 was treated with 0.426 µL seawater extract, and P3 was treated with 1.09 µL seawater extract. The result showed that determine significant differences between treatments. The post thawing membrane integrity’s result was P0= 22.00 ± 4.28, P1= 22.66 ± 3.61, P2= 25.00 ± 2.75, and P3= 29.00 ± 1.67. The post thawing acrosome cap’s result was P0= 30.50 ± 1.37, P1= 31.50 ± 3.27, P2= 34.83 ± 2.31, and P3= 38.00 ± 1.41. The highest concentration added seawater extract to increased membrane integrity and acrosome cap spermatozoa in this research was 1.09 µL. Keywords: Limousin bull; spermatozoa; seawater extract; membrane integrity; acrosome cap

Daya Motil dan Keutuhan Membran Plasma Spermatozoa Domba Garut (Ovis aries) Pada Penambahan Kolesterol Dalam Pengencer Semen Tris Kuning Telur

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Herdis
Keyword(s):  
Plasma Membrane ◽  
Semen Quality ◽  
Egg Yolk ◽  
Ovis Aries ◽  
Motile Sperm ◽  
Quality Of Control ◽  
Cholesterol Treatment ◽  
Frozen Semen ◽  
Artificial Vagina

The research was carried out to study the effect of egg-yolk consentration and cholesterol suplementationto tris - 1.2% maltosa extender on the quality of frozen semen of garut rams. Semen was collected once a week using artificial vagina from six mature Garut rams. Semen was equilibrated at 5oC for three hours, frozen and stored in liquid nitrogen. The thawing was carried out at the temperature 30oC for 30 seconds. Percentages of motility and intact plasma membrane (IPM) parameters were evaluated every stage of freezing semen. The results show that there were no interaction between both treatmenst on the quality of garut rams frozen semen. In the cholesterol treatment, percentages of progressive motile sperm and intact plasma membrane in control (50,50% and 57,92%) were significantly different (P<0,05) than cholesterol 1,0 mg /100 ml treatment (37,08% and 49,42%) respectively. In conclusion, addition of 10% egg yolk concentrationto Tris –1.2% maltosa extender was not significantly different than 20% egg yolk concentration. The frozen semen quality of control produced the best quality semen compared toboth addition of 0.5mg/100 ml and 1.0 mg/100 ml cholesterol treatments. Cholesterol addition to egg yolk Tris – 1.2% maltosa causes degradation of frozen semen quality.Penelitian dilakukan untuk melihat pengaruh konsentrasi kuning telur dan penambahan kolesterol pada pengencer semen Tris dengan maltosa 1,2% terhadap kualitas semen beku domba garut. Semen dikoleksi seminggu sekali dengan vagina buatan dari enam domba garut jantan. Semen diekuilibrasi pada suhu 5 oC selama tiga jam kemudian dibekukan dan disimpan di dalam nitrogen cair. Metode thawing dilakukan pada suhu 30oC selama 30 detik. Parameter persentase motilitas dan membran plasma utuh dievaluasi pada setiap tahap pembekuan. Hasil penelitian menunjukkan bahwa tidak ada interaksi antara kedua perlakuan terhadap kualitas semen beku domba garut. Perlakuan kolesterol menunjukan bahwa persentase motilitas dan membran plasma utuk pada perlakuan kontrol (50,50% dan 57,92%) lebih tinggi dan berbeda nyata dibandingkan perlakuan1,0 mg/100ml kolesterol (37,08% dan 49,42%). Penambahan kuning telur 10% pada pengencer Tris dengan maltosa 1,2% menghasilkan kualitas semen tidak berbeda dengan penambahan kuninf telur 20%, Penelitian menyimpulkan bahwa kualitas semen beku terbaik diperoleh pada perlakuan kontrol lebih tinggi dibandingkan perlakuan kolesterol 0,5 mg/100 ml dan 1,0 mg/100 ml. Penambahan kolesterol akan menurunkan kualitas semen beku domba garut.Keywords: cholesterol, motility, membrane plasm, garut ram, spermatozoa.

scholarly journals THE QUALITY OF BOAR FROZEN SEMEN DILUTED IN BTS® AND MII® WITH DIFFERENT CRYOPROTECTANT SUPPLEMENTED WITH SODIUM DODECYL SULPHATE

2017 ◽  
Vol 11 (1) ◽  
pp. 6-10
Author(s):  
Nancy Diana Frederika Katerina Foeh ◽  
Raden Iis Arifiantini ◽  
Tuty Laswardi Yusuf
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Sodium Dodecyl Sulphate ◽  
Semen Quality ◽  
Sodium Dodecyl ◽  
Sperm Concentration ◽  
Sperm Abnormalities ◽  
Semen Collection ◽  
Frozen Semen

This research was aimed to study the effect of administration of glycerol and dimetilacetamida (DMA) in BTS® and MIII®extender supplemented with sodium dodecyl sulphate (SDS) on boar frozen semen. A number of four boars were used in this study for semen collection (n=20). The collected semen was evaluated both macroscopically and microscopically. In this study, only the semen that demonstrated>70% sperm motility, >200.106/mL sperm concentration, and<20% sperm abnormalities were used and divided into eight tubes. A number of 4 tubes were diluted with 5 mL of BTS, and the rest with 5 mL MIII. The sampel was stored at 20-22° C for 2 hours, followed by centrifugation for 15 minutes (at 2000 rpm), and taken of pellet with 1 ml supernatant. The pellet that was resulted from centrifugation using BTS, then re-diluted with BTS-glycerol 5% (BTSG), BTS DMA 5% (BTSD), BTS-glycerol 5% and SDS (BTSG-S), BTS-DMA 5% and SDS (BTSD-S). Four other pellets that were centrifuged with MIII also re-diluted with MIII-glycerol 5% (MIIIG), MIII-DMA 5% (MIIID), MIII-glycerol 5% and SDS (MIIIG-S), MIII-DMA 5% and SDS (MIIID-S). Next, all of diluted semen were inserted into 0.5 mL straw and equilibrated for 2 hours (4° C), then frozen and stored in liquid nitrogen. The evaluation of frozen semen quality was conducted at 24 hours after frozen. The result of this study showed that post-thawing motility of spermatozoa in BTSD-S (40.17±0.2%) was found higher (P<0.05) compared to seven other dilution processes. Therefore, it is concluded that the concentration of 5% DMA that supplemented with SDS in BTS dilution much better for maintaining boars frozen semen quality.

scholarly journals Pengaruh Level Glutathione terhadap Kualitas Post-Thawing Semen Kambing Peranakan Etawah

2020 ◽  
Vol 7 (2) ◽  
pp. 138
Author(s):  
Nurcholidah Solihati ◽  
Soeparna Soeparna ◽  
Siti Darodjah Rasad ◽  
Rangga Setiawan ◽  
Annisaa Yusrina
Keyword(s):  
Lipid Peroxidation ◽  
Plasma Membrane ◽  
Semen Quality ◽  
Glutathione Level ◽  
Negative Effects ◽  
Test Parameter ◽  
Randomized Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAK Glutathione merupakan antioksidan yang berfungsi melindungi sel dari kerusakan akibat oksigen reaktif (ROS) sehingga dapat mencegah reaksi peroksidasi lipid. Penggunaan glutathione dalam pengencer harus sesuai supaya tidak menimbulkan efek negatif. Penelitian ini bertujuan untuk mengetahui pengaruh level glutathione terhadap kualitas semen kambing Peranakan Etawah post thawing dan mengetahui level glutathione yang menghasilkan kualitas semen yang terbaik. Metode penelitian yang digunakan adalah percobaan laboratorium. Penelitian ini mengunakan rancangan acak kelompok dengan lima kelompok kambing dan lima level glutathione (0, 4, 6, 8 dan 10 mM), setiap perlakuan diulang dua kali. Semen ditampung dengan vagina buatan dan dievaluasi secara makroskopis dan mikroskopis, selanjutnya dilakukan pengolahan menjadi semen beku Data dianalisis dengan analisis ragam dan perbedaan antar perlakuan diuji dengan uji lanjut Duncan. Parameter yang diamati adalah kualitas semen post-thawing meliputi motilitas, abnormalitas, membran plasma utuh dan tudung akrosom utuh (TAU). Hasil penelitian menunjukkan bahwa perlakuan level glutathione berpengaruh nyata (p<0,05) terhadap kualitas semen post-thawing. Hasil uji Duncan menunjukkan bahwa motilitas, TAU dari perlakuan level glutathione 6 mM dan 8 mM tidak berbeda nyata, namun MPU hasil 8 mM nyata lebih tinggi dari level lainnya. Disimpulkan bahwa level glutathione berpengaruh terhadap kualitas semen post-thawing, dan level glutathione 8 mM menghasilkan kualitas semen yang terbaik.Kata kunci: glutathione, kualitas semen, post-thawing, kambing peranakan etawahABSTRACT Glutathione is an antioxidant that functions to protect cells from damage caused by reactive oxygen (ROS) so that it can prevent lipid peroxidation reactions. The use of glutathione in diluents must be suitable so as not to cause negative effects The aim of the research was to knew the effect of glutathione level on semen quality of Etawah Crossbreed goat and to know the level of glutathione that produce the best post-thawed semen quality. This research used grouped randomized design with five groups of goat and five glutathione levels (0, 4, 6, 8, and 10 mM), every treatment was repeated twice. Semen was collected with an artificial vagina and was evaluated as macroscopic and microscopic, they were being processed as frozen semen. Data were analyzed with analysis of variance and differences between treatments were analyzed using the Duncan test. Parameter was observed is pot-thawed semen quality consist of motility, abnormality, intact plasma membrane (IPM) and intact acrosome cup (IAC). The result showed that treatment of glutathione level significantly (p<0.05) affect on post-thawed semen quality. Duncan test showed that motility and IAC from treatment glutathione of 6 mM and 8mM were not significant, nevertheless IPM from treatment 8 mM glutathione significantly higher than other levels. It is concluded that the glutathione level significantly affect on post-thawed semen quality, and 8 mM glutathione level resulted in the best quality.Keywords:  glutathione, semen quality, post-thawed, etawah crossbreed goat

scholarly journals The The Effect of Age and Breed on The Quality of Bull Semen in The Regional Artificial Insemination Centre

2021 ◽  
pp. 132-136
Author(s):  
Agung Budiyanto ◽  
Makruf Arif ◽  
Mandala Phivi Whelma Alfons ◽  
Rifia Tiara Fani ◽  
Ardian Faiz Hafid ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Age Groups ◽  
Bull Semen ◽  
Sperm Abnormalities ◽  
Frozen Semen ◽  
Cattle Reproduction ◽  
Effect Of Age ◽  
Artificial Vagina

The success of beef cattle reproduction development in Indonesia very much depends on the quality of the semen produced by the Indonesian Institute for Artificial Insemination. Good quality bull will benefit farmers, frozen semen producers, and the country in large. This study aimed to determine effect of age and breed on the libido and quality of bull semen collected in the Regional Artificial Insemination Center (RAIC). This study used PO, Simmental, and Brahman Bull in RAIC Yogyakarta, with a total amount of 15, aged 4-7 years old. Semen was collected using an artificial vagina, semen evaluation used a spectrophotometer, and eosin-nigrosin staining method used for sperm viability and abnormalities examination. The result of this study indicated that bull breed did not affect the libido value and the semen quality of bulls (P>0,05). Volume, concentration, motility, and viability in various age groups did not show significant differences (P>0,05), but age groups had a significant effect on sperm abnormalities (P<0,05). The group of bulls aged 5-6 years showed the best quality compared to other groups, with the volume 4,58 ± 1,37 ml, concentration 1,91 ± 0,36 x109/ml, motility 3,80 ± 1,30, viability 89.83 ± 10.69%, and sperm abnormalities 2.51 ± 0.81%. It can be concluded that breed did not affect the bull libido and semen quality, while age had an effect on bull sperm abnormalities.

Quality of ram spermatozoa separated with modified swim up method

2018 ◽  
Vol 33 (2) ◽  
pp. 62-70 ◽  
Author(s):  
A Hossain ◽  
MM Islam ◽  
F Naznin ◽  
RN Ferdousi ◽  
FY Bari ◽  
...  
Keyword(s):  
Semen Quality ◽  
Membrane Integrity ◽  
Quality Parameters ◽  
Fluid Medium ◽  
Normal Morphology ◽  
The Mean ◽  
Mass Activity ◽  
Fresh Semen ◽  
Artificial Vagina

Semen was collected from four rams, using artificial vagina and viability%, motility% and plasma membrane integrity% were measured. Fresh ejaculates (n = 32) were separated by modified swim-up separation using modified human tubal fluid medium. Four fractions of supernatant were collected at 15-minute intervals. The mean volume, mass activity, concentration, motility%, viability%, normal morphology and membrane integrity% (HOST +ve) of fresh semen were 1.0 ± 0.14, 4.1 ± 0.1 × 109 spermatozoa/ml, 85.0 ± 1.3, 89.4 ± 1.0, 85.5 ± 0.7, 84.7 ± 0.5 respectively. There was no significant (P>0.05) difference in fresh semen quality parameters between rams. The motility%, viability% and HOST +ve % of first, second, third and fourth fractions were 53.4 ± 0.5, 68.2 ± 0.3, 74.8 ± 0.3 and 65.5 ± 0.4; 55.5 ± 0.4, 66.2 ± 0.4, 74.5 ± 0.3 and 73.6 ± 0.3 and 66.7 ± 0.5, 66.8 ± 0.5, 65.2 ± 0.4 and 74.7 ± 0.5 respectively. The motility%, viability% and membrane integrity% of separated semen samples differed significantly (P<0.05) between four fractions. The mean motility% and viability% were significantly higher (P<0.05) in third fraction (74.8 ± 0.3%), whereas the mean HOST +ve% was significantly higher (P<0.05) in fourth fraction (74.7 ± 0.5). All quality parameters of separated spermatozoa were significantly (P<0.05) lower than that of fresh semen. The pregnancy rates were higher with fresh semen (71%) in comparison to that of separated sample (57%).Bangl. vet. 2016. Vol. 33, No. 2, 62-70

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