scholarly journals Pengaruh Level Glutathione terhadap Kualitas Post-Thawing Semen Kambing Peranakan Etawah

2020 ◽  
Vol 7 (2) ◽  
pp. 138
Author(s):  
Nurcholidah Solihati ◽  
Soeparna Soeparna ◽  
Siti Darodjah Rasad ◽  
Rangga Setiawan ◽  
Annisaa Yusrina
Keyword(s):  
Lipid Peroxidation ◽  
Plasma Membrane ◽  
Semen Quality ◽  
Glutathione Level ◽  
Negative Effects ◽  
Test Parameter ◽  
Randomized Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAK Glutathione merupakan antioksidan yang berfungsi melindungi sel dari kerusakan akibat oksigen reaktif (ROS) sehingga dapat mencegah reaksi peroksidasi lipid. Penggunaan glutathione dalam pengencer harus sesuai supaya tidak menimbulkan efek negatif. Penelitian ini bertujuan untuk mengetahui pengaruh level glutathione terhadap kualitas semen kambing Peranakan Etawah post thawing dan mengetahui level glutathione yang menghasilkan kualitas semen yang terbaik. Metode penelitian yang digunakan adalah percobaan laboratorium. Penelitian ini mengunakan rancangan acak kelompok dengan lima kelompok kambing dan lima level glutathione (0, 4, 6, 8 dan 10 mM), setiap perlakuan diulang dua kali. Semen ditampung dengan vagina buatan dan dievaluasi secara makroskopis dan mikroskopis, selanjutnya dilakukan pengolahan menjadi semen beku Data dianalisis dengan analisis ragam dan perbedaan antar perlakuan diuji dengan uji lanjut Duncan. Parameter yang diamati adalah kualitas semen post-thawing meliputi motilitas, abnormalitas, membran plasma utuh dan tudung akrosom utuh (TAU). Hasil penelitian menunjukkan bahwa perlakuan level glutathione berpengaruh nyata (p<0,05) terhadap kualitas semen post-thawing. Hasil uji Duncan menunjukkan bahwa motilitas, TAU dari perlakuan level glutathione 6 mM dan 8 mM tidak berbeda nyata, namun MPU hasil 8 mM nyata lebih tinggi dari level lainnya. Disimpulkan bahwa level glutathione berpengaruh terhadap kualitas semen post-thawing, dan level glutathione 8 mM menghasilkan kualitas semen yang terbaik.Kata kunci: glutathione, kualitas semen, post-thawing, kambing peranakan etawahABSTRACT Glutathione is an antioxidant that functions to protect cells from damage caused by reactive oxygen (ROS) so that it can prevent lipid peroxidation reactions. The use of glutathione in diluents must be suitable so as not to cause negative effects The aim of the research was to knew the effect of glutathione level on semen quality of Etawah Crossbreed goat and to know the level of glutathione that produce the best post-thawed semen quality. This research used grouped randomized design with five groups of goat and five glutathione levels (0, 4, 6, 8, and 10 mM), every treatment was repeated twice. Semen was collected with an artificial vagina and was evaluated as macroscopic and microscopic, they were being processed as frozen semen. Data were analyzed with analysis of variance and differences between treatments were analyzed using the Duncan test. Parameter was observed is pot-thawed semen quality consist of motility, abnormality, intact plasma membrane (IPM) and intact acrosome cup (IAC). The result showed that treatment of glutathione level significantly (p<0.05) affect on post-thawed semen quality. Duncan test showed that motility and IAC from treatment glutathione of 6 mM and 8mM were not significant, nevertheless IPM from treatment 8 mM glutathione significantly higher than other levels. It is concluded that the glutathione level significantly affect on post-thawed semen quality, and 8 mM glutathione level resulted in the best quality.Keywords:  glutathione, semen quality, post-thawed, etawah crossbreed goat

Daya Motil dan Keutuhan Membran Plasma Spermatozoa Domba Garut (Ovis aries) Pada Penambahan Kolesterol Dalam Pengencer Semen Tris Kuning Telur

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Herdis
Keyword(s):  
Plasma Membrane ◽  
Semen Quality ◽  
Egg Yolk ◽  
Ovis Aries ◽  
Motile Sperm ◽  
Quality Of Control ◽  
Cholesterol Treatment ◽  
Frozen Semen ◽  
Artificial Vagina

The research was carried out to study the effect of egg-yolk consentration and cholesterol suplementationto tris - 1.2% maltosa extender on the quality of frozen semen of garut rams. Semen was collected once a week using artificial vagina from six mature Garut rams. Semen was equilibrated at 5oC for three hours, frozen and stored in liquid nitrogen. The thawing was carried out at the temperature 30oC for 30 seconds. Percentages of motility and intact plasma membrane (IPM) parameters were evaluated every stage of freezing semen. The results show that there were no interaction between both treatmenst on the quality of garut rams frozen semen. In the cholesterol treatment, percentages of progressive motile sperm and intact plasma membrane in control (50,50% and 57,92%) were significantly different (P<0,05) than cholesterol 1,0 mg /100 ml treatment (37,08% and 49,42%) respectively. In conclusion, addition of 10% egg yolk concentrationto Tris –1.2% maltosa extender was not significantly different than 20% egg yolk concentration. The frozen semen quality of control produced the best quality semen compared toboth addition of 0.5mg/100 ml and 1.0 mg/100 ml cholesterol treatments. Cholesterol addition to egg yolk Tris – 1.2% maltosa causes degradation of frozen semen quality.Penelitian dilakukan untuk melihat pengaruh konsentrasi kuning telur dan penambahan kolesterol pada pengencer semen Tris dengan maltosa 1,2% terhadap kualitas semen beku domba garut. Semen dikoleksi seminggu sekali dengan vagina buatan dari enam domba garut jantan. Semen diekuilibrasi pada suhu 5 oC selama tiga jam kemudian dibekukan dan disimpan di dalam nitrogen cair. Metode thawing dilakukan pada suhu 30oC selama 30 detik. Parameter persentase motilitas dan membran plasma utuh dievaluasi pada setiap tahap pembekuan. Hasil penelitian menunjukkan bahwa tidak ada interaksi antara kedua perlakuan terhadap kualitas semen beku domba garut. Perlakuan kolesterol menunjukan bahwa persentase motilitas dan membran plasma utuk pada perlakuan kontrol (50,50% dan 57,92%) lebih tinggi dan berbeda nyata dibandingkan perlakuan1,0 mg/100ml kolesterol (37,08% dan 49,42%). Penambahan kuning telur 10% pada pengencer Tris dengan maltosa 1,2% menghasilkan kualitas semen tidak berbeda dengan penambahan kuninf telur 20%, Penelitian menyimpulkan bahwa kualitas semen beku terbaik diperoleh pada perlakuan kontrol lebih tinggi dibandingkan perlakuan kolesterol 0,5 mg/100 ml dan 1,0 mg/100 ml. Penambahan kolesterol akan menurunkan kualitas semen beku domba garut.Keywords: cholesterol, motility, membrane plasm, garut ram, spermatozoa.

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

scholarly journals THE PROPORTION OF X AND Y SPERM, VIABILITY AND MOTILITY OF RAM SPERMATOZOA AFTER SEPARATED WITH WHITE EGG ALBUMIN

2017 ◽  
Vol 41 (1) ◽  
pp. 1 ◽  
Author(s):  
Moh Takdir ◽  
Ismaya Ismaya ◽  
Sigit Bintara
Keyword(s):  
Egg White ◽  
Egg Albumin ◽  
Randomized Design ◽  
Frozen Semen ◽  
Bottom Fraction ◽  
Completely Randomized Design ◽  
Ram Sperm ◽  
Artificial Vagina ◽  
Range Test

ABSTRACT The aim of this research was to determine the proportion, viability and motility of X and Y ram spermatozoa separated with egg white albumin. Sperm samples derived from Garut ram, which was collected by using an artificial vagina. Observations were made on spermatozoa fraction above and below each medium fraction treatment. There are treatment egg white albumin as separation medium, each medium consisting of fractions top and bottom fraction with different concentration: 1) P0 = sperma before separation (control); 2) P1 = 10% above fraction + 30% lower fraction; P2 = 25% + 45%; P3 = 25% + 75%. Data proportion of X and Y, viability and motility were analyzed statistically by Completely Randomized Design patern in the direction followed by Duncan’s Multiple Range Test for data with a real difference. Separation with egg white albumin affect significantly increased the proportion of spermatozoa X and Y (P≤0.05), but tends to decrease the viability and motility of spermatozoa.The proportion of spermatozoa X and Y was highest in treatment P3,76.76% of spermatozoa X (fraction above 25%) and 79.81% spermatozoa Y (75% lower fraction), with an average viability obtained respectively 68,9% (fraction above) and 59,7% (bottom fraction), motility 77,5% (fraction above) dan 84,0% (bottom fraction). It was concluded that the egg white albumin is very effective in changing the proportions of X and Y ram sperm with the quality of spermatozoa after separation feasible for applications insemination or processed into frozen semen.  (Keywords: Garut ram, White egg albumin, Spermatozoa X and Y) 

scholarly journals Effects of the addition of docosahexaenoic acid and ?-tocopherol on quality of equine spermatozoa stored at 5°C

2020 ◽  
Vol 41 (1) ◽  
pp. 167 ◽  
Author(s):  
Breno Fernandes Barreto Sampaio ◽  
Bruno Gomes Nogueira ◽  
Maria Inês Lenz Souza ◽  
Eliane Vianna da Costa-e-Silva ◽  
Carmem Estefânia Serra Neto Zúccari
Keyword(s):  
Lipid Peroxidation ◽  
Plasma Membrane ◽  
Docosahexaenoic Acid ◽  
Sperm Motility ◽  
Membrane Integrity ◽  
Control Group ◽  
Mitochondrial Activity ◽  
Membrane Composition ◽  
Artificial Vagina

Plasma membrane composition has impact on phase transition from liquid crystal to gel state of cooled sperm cell. The incorporation of polyunsaturated fatty acids increases its fluidity and can contribute to sperm motility. The aim of this study was to compare the effect of adding docosahexaenoic acid (DHA) and ?-tocopherol (?-Toh) to the cooling extender, singly or combined, to the equine sperm parameters, submitted to cooling, up to 72 hours. Two ejaculates of ten stallions collected with artificial vagina were used, and evaluated for motility, plasma membrane integrity, chromatin fragmentation, mitochondrial activity and lipid peroxidation, according to the following treatments: C; DHA; ?-Toh; DHA/?-Toh; EtOH 100: and EtOH 140 (corresponding to control; 10 ng mL-1 of DHA; 2 mM of ?-Toh; : 10 ng mL-1 of DHA + 2 mM of ?-Toh; 100 µL of ethanol and 140 µL of ethanol respectively). DHA treatment showed higher motility (68.2 ± 12.3; p < 0.05) when compared to control (62.1 ± 16.2), DHA/?-Toh (61.3 ± 12.7) and EtOH (58.1 ± 8.6) groups. In lipid peroxidation assay, the control group showed 2,506.2 ± 796.4 ng of MDA 108 spermatozoa-1, being significantly higher (p < 0.05) than the groups treated with DHA (2,036.0 ± 687.0), ?-Toh (1,890.8 ± 749.5) and DHA/?-Toh (1,821.1 ± 627.2). In conclusion, ?-Toh was effective in diminishing lipid peroxidation of equine sperm subjected to cooling, and DHA improved sperm motility and, in spite of being a polyunsaturated fatty acid with high susceptibility to peroxidation, reduced lipid peroxidation.

scholarly journals The Influence of Breed and Type of Extender on the Quality of Bull Semen

2020 ◽  
Vol 21 (2) ◽  
pp. 64
Author(s):  
Iman Sukirman ◽  
Eros Sukmawati ◽  
Siti Darojah Rasad ◽  
Nurcholidah Solihati
Keyword(s):  
Plasma Membrane ◽  
Semen Quality ◽  
Block Design ◽  
Egg Yolk ◽  
Spermatozoa Motility ◽  
Bull Semen ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Two Factors

This study aimed to determine the influence of breed and type of extenders on frozen semen quality of cows at BIB Lembang. The experimental study was conducted in a Factorial Randomized Block Design (RBD) with two factors. The first factor was four cow breeds, i.e. Ongole Cross (PO), Brahman (BR), Simmental (SM) and Limousin (LM), and the second factor was two types of extender, i.e. Skim-Egg Yolk (SKT) and AndroMed® (AND), all repeated four times. The observed variables were percentage of spermatozoa motility and intact plasma membrane (IPM). All data obtained were analyzed using a general linear model (IBM SPSS ver. 23). The results demonstrated an interaction between breed and the type of diluent to motility. Breeds showed significantly different motility but non-significantly different intact plasma membrane (MPU) of semen. The type of diluent did not significantly affect motility and intact plasma membrane (MPU) of the frozen semen. The effect of the breed on BR motility was lower and significantly different from PO, LM and SM. The types of diluent did not significantly affect motility, MPU. The results showed that SKT was lower than AND, it was indicative effect of breed on intact plasma membrane (MPU) PO was lower than BR, LM and SM and the effect of the type of diluent on whole plasma membrane (MPU) AND is lower than SKT. It can be concluded that breed influences the motility of semen. The lowest motility reduction in frozen semen is Brahman cattle by using skim-egg yolk extender.

scholarly journals Effect of Monthly Variations on the Plasma Membrane, Acrosome and DNA Integrities of Spermatozoa in Friesian Holstein Bulls Born in Iraq

2020 ◽  
Vol 13 (2) ◽  
pp. 118-124
Author(s):  
Qusay Aboud ◽  
Saad Hatif
Keyword(s):  
Plasma Membrane ◽  
Artificial Insemination ◽  
Significant Decline ◽  
Dna Integrity ◽  
Frozen Semen ◽  
Negative Effect ◽  
Holstein Bulls ◽  
Monthly Variations ◽  
Artificial Vagina

This study was aimed to evaluate the influence of months of the year on the quality of semen in Holstein bulls. A study carried out at artificial insemination centre/ Abou-Ghareeb/ western of Baghdad. A total of 160 ejaculates were collected from 15 bulls born in Iraq via the artificial vagina. The age of the bulls ranged between 4 to 5 years and the study period were December to March and September. The semen samples were diluted with Tris base extender. The semen was packed in a straw according to the program of artificial insemination centre. Semen characteristics (plasma membrane, acrosome, and DNA integrities) were evaluated. The results revealed a significant decrease (P≤0.01) in the plasma membrane and acrosome integrity in September as compared with December, January, February, and March. There was a significant decline (P≤0.05) in DNA integrity in September as compared with December, January, February, and March in fresh and frozen semen. In conclusion, the September month had a negative effect on the plasma membrane, acrosome, and DNA percentage in all bulls.

scholarly journals Increased Integrity of Plasma Membrane and Acrosome Cap Spermatozoa Limousin Cattle at Post Thawing in Frozen Media by adding Seawater Extract

2017 ◽  
Vol 3 (6) ◽  
pp. 633
Author(s):  
Nur Faidah ◽  
Tatik Hernawati ◽  
Mirni Lamid ◽  
Ismudiono Ismudiono ◽  
Tri Wahyu Suprayogi ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Experimental Design ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Skim Milk ◽  
Egg Yolk ◽  
Duncan Multiple Range Test ◽  
Frozen Semen ◽  
Artificial Vagina ◽  
Range Test

This research was determined membrane integrity and acrosome cap of Limousin bull post thawing after adding seawater extract with different concentrations in extender skim milk and egg yolk to increased frozen semen quality. This research used fresh samples of Limousin bull’s semen collected by using artificial vagina, then devided into 4 treatments and 6 replications. The experimental design that used was Complete Random Design. Analysis of the data using Analysis of Variant (ANOVA) one way then proceeds to the Duncan Multiple Range Test to determine significant differences between treatments.The first treatment P0 no seawater extract added as a control. The second treatment P1 was treated with 0.109 µL seawater extract, P2 was treated with 0.426 µL seawater extract, and P3 was treated with 1.09 µL seawater extract. The result showed that determine significant differences between treatments. The post thawing membrane integrity’s result was P0= 22.00 ± 4.28, P1= 22.66 ± 3.61, P2= 25.00 ± 2.75, and P3= 29.00 ± 1.67. The post thawing acrosome cap’s result was P0= 30.50 ± 1.37, P1= 31.50 ± 3.27, P2= 34.83 ± 2.31, and P3= 38.00 ± 1.41. The highest concentration added seawater extract to increased membrane integrity and acrosome cap spermatozoa in this research was 1.09 µL. Keywords: Limousin bull; spermatozoa; seawater extract; membrane integrity; acrosome cap

scholarly journals KUALITAS SEMEN BEKU DOMBA GARUT (Ovis aries) PENAMBAHAN SUKROSA DALAM PENGENCER SEMEN KUNING

2017 ◽  
Vol 16 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Herdis Suharman
Keyword(s):  
Plasma Membrane ◽  
Sperm Motility ◽  
Ovis Aries ◽  
Initial Evaluation ◽  
Freezing Process ◽  
Frozen Semen ◽  
Semen Extender ◽  
Fresh Semen ◽  
Artificial Vagina

The objective of this research was to examine the effect of sucrose in improving the quality of the plasma membrane intact and sperm motility of frozen semen of Garut ram. Semen was collected using artificial vagina weekly from six mature garut rams.   Immediately after initial evaluation, fresh semen was divided into four parts and diluted with Tr s extender without sucrose (T0), Tris extender + sucrose 0.2g/100 ml (T1), Tris extender + sucrose 0.4g/100 ml (T2) and Tris extender + sucrose 0.6g/100 ml (T3), respectively.  Results of this research showed that the percentage of sperm motility after thawing in T2 (49.00 ± 5.48%)  was significantly (P<0.05) higher than T0 (42.00 ± 2.74%) but was not significantly difference (P>0.05) than T1 (46.00 ± 4.18%)and T3 (48.00 ± 4.47%).   Evaluation of plasma membrane intact showed that T1 (62.33 ± 6.51%) was s gnificantly different (P <0.05) w h T0 (49.40 ± 2.19%) but was not significantly different (P> 0.05) than T2 (58.50 ± 4.97%) and T3 (56.40 ± 5.90%).  In conclusion, he addition of sucrose in semen extender  improved the quality of frozen semen of Garut ram.  Concentration of 0.2g / 100 ml is the op ma dose to improve the quality of the plasma membrane intact and motility of spermatozoa during the freezing process.

scholarly journals Effect of Incubation Time During Sperm Sexing Process on Sperm Quality of Pasundan Bull

2020 ◽  
Vol 25 (3) ◽  
pp. 112
Author(s):  
Siti Darodjah Rasad ◽  
Nurcholidah Solihati ◽  
Kikin Winangun ◽  
Annisa Yusrina ◽  
Fahmy Avicenna
Keyword(s):  
Plasma Membrane ◽  
Incubation Time ◽  
Lower Layer ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Dna Integrity ◽  
Test Parameter ◽  
Plasma Membrane Integrity ◽  
Randomized Design

The research was conducted to evaluate the effect of incubation time on viability, plasma membrane integrity, abnormality, and DNA integrity of sexed Pasundan’s bulls sperm. The sperm sexing used 5% and 10% concentrations of Bovine Serum Albumin (BSA).  A completely randomized design with three treatments and six replications was used in this study. The data were analyzed using variance analysis followed by Duncan’s multiple distance test.  Parameter evaluated were sperm longevity, plasma membrane integrity (PMI), abnormality, and DNA integrity of sexed Pasundan bulls sperm. Results showed that incubation time gave significant effect (P&lt;0.05) on the longevity of sperm, but not on the PMI of Pasundan bulls sexed sperm.  The incubation time of 45 minutes gave the highest value of longevity sperm on the upper layer (4.33 days) and the lower layer (4.17 days). Furthermore, the abnormality of sperm X in the upper layer was 4.00%-4.20% and the lower layer was 4.10%- 4.40%.  Meanwhile, the DNA integrity of an upper layer was 98.16%-98.66%, and the lower layer was 97.83%-98.58%.  It is concluded that 45 minutes of incubation time significantly affected the longevity of sperm, but not plasma membrane integrity, abnormality, and DNA integrity of Pasundan bulls sexed sperm.

PENGARUH MALTOSA SEBAGAI KRIOPROTEKTAN EKSTRASELULER DALAM MENINGKATKAN KUALITAS SEMEN BEKU GUNA MENDUKUNG KEBERHASILAN TEKNOLOGI INSEMINASI BUATAN

2013 ◽  
Vol 14 (3) ◽  
Author(s):  
Herdis Herdis ◽  
I wayan Angga Darmawan
Keyword(s):  
Plasma Membrane ◽  
Liquid Nitrogen ◽  
Optimal Dose ◽  
Motile Sperm ◽  
Frozen Semen ◽  
Viable Sperm ◽  
Artificial Vagina

The research carried out to observe the effect of maltose addition on the quality of frozen semen of garut rams. Semen was collected once a week using artificial vagina from six mature garut rams. Semen was equilibrated at 5oC for four hours, frozen and stored in liquid nitrogen. The thawing was carried out at the temperature 30oC for 30 seconds. The result showed that percentages of viable sperm for addition of maltose 1,2 g / 100 ml extender (68,50 ± 0,84%) was significantly difference (P<0,05) than control (54,83 ± 1,94%) and addition of maltose 0,6 g / 100 ml extender (65,67 ± 1,03 %). The percentages of progressive motile sperm and percentages of plasma membrane for addition of maltose 1,2 g / 100 ml extender (53% and 64,67%) were significantly difference (P<0,05) than control (43% and 53,83%) but were not significantly different (P>0,05) from addition of maltose 0,6 g / 100 ml extender (50,83% and 64,67%)respectively. In conclusion, the addition of maltose 1,2 g / 100 ml extender is optimal dose to improve the quality of frozen semen of garut rams.

Sign in / Sign up

Export Citation Format

Share Document