Production, immobilization, and application de invertase from new wild strain Cunninghamella echinulata PA3S12MM

2021 ◽  
Author(s):  
Letícia Mara Rasbold ◽  
Vitória Maciel Delai ◽  
Caroline Marlene da Cruz Kerber ◽  
Márcia Regina Simões ◽  
Paulo Ricardo Heinen ◽  
...  
Keyword(s):  
Wild Strain ◽  
Cunninghamella Echinulata

Antagonistic activity of Bacillus subtilis strains isolated from various sources

2018 ◽  
Vol 8 (2) ◽  
pp. 354-364
Author(s):  
A. N. Irkitova ◽  
A. V. Grebenshchikova ◽  
A. V. Matsyura
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Wild Strain ◽  
Fish Farming ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
E Coli ◽  
Long Period ◽  
Test Culture ◽  
Agar Media

<p>An important link in solving the problem of healthy food is the intensification of the livestock, poultry and fish farming, which is possible only in the adoption and rigorous implementation of the concept of rational feeding of animals. In the implementation of this concept required is the application of probiotic preparations. Currently, there is an increased interest in spore probiotics. In many ways, this can be explained by the fact that they use no vegetative forms of the bacilli and their spores. This property provides spore probiotics a number of advantages: they are not whimsical, easily could be selected, cultivated, and dried. Moreover, they are resistant to various factors and could remain viable during a long period. One of the most famous spore microorganisms, which are widely used in agriculture, is <em>Bacillus subtilis</em>. Among the requirements imposed to probiotic microorganisms is mandatory – antagonistic activity to pathogenic and conditional-pathogenic microflora. The article presents the results of the analysis of antagonistic activity of collection strains of <em>B. subtilis</em>, and strains isolated from commercial preparations. We studied the antagonistic activity on agar and liquid nutrient medias to trigger different antagonism mechanisms of <em>B. subtilis</em>. On agar media, we applied three diffusion methods: perpendicular bands, agar blocks, agar wells. We also applied the method of co-incubating the test culture (<em>Escherichia coli</em>) and the antagonist (or its supernatant) in the nutrient broth. Our results demonstrated that all our explored strains of <em>B. subtilis</em> have antimicrobial activity against a wild strain of <em>E. coli</em>, but to varying degrees. We identified strains of <em>B. subtilis</em> with the highest antagonistic effect that can be recommended for inclusion in microbial preparations for agriculture.</p><p><em><br /></em><em></em></p>

Accelerated Phenol Removal by Amplifying the Metabolic Pathway with a Recombinant Plasmid Encoding Catechol 2, 3 Oxygenase

1992 ◽  
Vol 26 (9-11) ◽  
pp. 2191-2194 ◽  
Author(s):  
M. Fujita ◽  
M. Ike ◽  
T. Kamiya
Keyword(s):  
Metabolic Pathway ◽  
Recombinant Plasmid ◽  
Removal Rate ◽  
Phenol Degradation ◽  
Wild Strain ◽  
Phenol Concentration ◽  
Phenol Removal

The metabolic pathway of the phenol degradation in Pseudomonasputida BH was amplified by introducing the recombinant plasmid containing catechol 2,3 oxygenase gene isolated fron the chromosome of BH. This strain could degrade phenol and grow much faster than the wild strain at the phenol concentration of 100mg/L. This strain seems to accelerate the phenol removal rate if it is applied to the treatment of wastewater containing phenol.

Understanding Process Variables and their Interactions for Maximizing Production of Artemisinin Derivative Artemether (Anti-Malarial Drug) Through Cunninghamella echinulata var elegans at 5 L Bioreactor Level

2019 ◽  
Vol 15 (4) ◽  
pp. 442-452
Author(s):  
Kashyap Kumar Dubey ◽  
Punit Kumar
Keyword(s):  
Experimental Finding ◽  
Culture Broth ◽  
Quadratic Model ◽  
Optimum Conditions ◽  
Reverse Phase Hplc ◽  
Polar Solvents ◽  
Reverse Phase ◽  
Experimental Conditions ◽  
Cunninghamella Echinulata ◽  
Life Threatening

Background: Malaria is one of the life threatening diseases which is caused by Plasmodium sp. of protozoa and uses Anopheles mosquitos as vector. Plasmodium vivax and Plasmodium falciparum are common form of malaria parasite. Artemisinin is reported for its antimalarial activities and Artemether which is a methyl ether derivative of Artemisinin, has been found effective against P. falciparum. Methods: In the present study, bioconversion of Artemisinin into Artemether was carried out experimentally and the statistical tools like experimental factorial design and Response Surface Methodology were used to find optimal conditions (concentration of Artemisinin, age of inoculum, temperature & pH) using Cunninghamella echinulata var. elegans. Experimental conditions for maximum product recovery from culture broth were also optimized using various polar and non-polar solvents for extraction. Artemether purity was analyzed by reverse-phase HPLC. Experimental data was fitted in a quadratic model and effect of various parameters was analyzed. Results: It was found that bioconversion of Artemisinin into Artemether is growth associated process. It was observed that molasses used as carbon source supported production of Artemether to 3.4g/L. The biomass and oxygen are key element affecting of bioconversion of Artemisinin into Artemether such as higher dissolved oxygen reduced the Artemether bioconversion. The highest bioconversion of Artemisinin into Artemether was obtained at temperature 25.5oC, 5g/L concentration of Artemisinin, at age of inoculum of 44.5 h and at pH 6.0. Model suggested the highest bioconversion of Artemisinin into Artemether was 54% at shake flask level which was near about experimental finding. An optimal condition for bioconversion was also analyzed and 64% bioconversion was obtained in 5L bioreactor. Conclusion: The outcomes of the study provided optimum conditions for bioconversion of Artemisinin into Artemether.

PRODUÇÃO SIMULTÂNEA DE BIOMASSA E LIPÍDEOS UTILIZANDO MEIOS CONTENDO RESÍDUOS AGROINDUSTRIAIS POR Mucor subtilíssimus UCP/WFCC 1262, Cunninghamella echinulata UCP/WFCC 1299 e Rhizopus microsporus UCP/WFCC 1304 ISOLADOS DO SOLO DA CAATINGA DE PERNAMBUCO

Engevista ◽  
2017 ◽  
Vol 19 (5) ◽  
pp. 1417
Author(s):  
Miller Da Costa Lima ◽  
Tayane de Cássia Dias Mendes Silva ◽  
Adriana Ferreira De Souza ◽  
Marcos Antônio Cavalcanti Luna ◽  
Rosileide Fontenele Silva Andrade ◽  
...  
Keyword(s):  
Rhizopus Microsporus ◽  
Cunninghamella Echinulata

A produção de lipídeos microbianos oferece vantagens em relação aos vegetais e animais, apresentando rápida geração, não necessitando de grandes áreas de terra arável e tendo melhor controle da produção e do produto. Nesse sentido, o objetivo deste trabalho foi realizar uma seleção de fungos mucorales (Mucor subtilíssimus UCP/WFCC 1262, Cunninghamella echinulata UCP/WFCC 1299 e Rhizopus microsporus UCP/WFCC 1304), isolados do solo da Caatinga de Pernambuco em meio contendo resíduos agroindustriais. O cultivo foi realizado em diferentes concentrações de melaço de cana de açúcar, milhocina e pH. A linhagem com elevado potencial em produzir biomassa e lipídeos foi cultivada em diferentes níveis, a partir do meio selecionado, utilizando planejamento fatorial 2³. Os rendimentos das biomassas foram calculados por gravimetria, os lipídeos totais quantifidados após extração por clorofórmio e metanol e a identificação dos ácidos graxos foi realizada por cromatografia gasosa (GC). Adicionalmente, um estudo histoquímico foi realizado com o fungo selecionado. Os resultados obtidos demonstraram que Cunninghamella echinulata foi o micro-organismo selecionado devido a elevada produção simultanea de biomassa (9,05g/L) e lipídeos totais (46,96%) em meio consituído por 10% de melaço de cana de açúcar e 5% de milhocina, com o pH 6. A partir deste meio selecionado foi realizado um planejamento fatorial 2³ para cultivo com a Cunninghella echinulata visando obter a máxima produção de biomassa e lipídeos totais. De acordo com o planejamento o meio constituído por milhocina (8%), melaço de cana de açúcar (12%) e pH 6.0 (ensaio 4), foi obtida a máxima concentração de biomassa (10,1g/L) e lipídeos totais (47,86%).  A partir da biomassa de Cunninghamella echinulata cultivada no meio selecionado do planejamento fatorial foi observado o máximo acúmulo de lipídeo no micélio, confirmando os resultados obtidos neste estudo para a produção de lipídeos.

A Comparison of Productivity in a Mutant and Wild Strain of Tribolium castaneum Herbst

1958 ◽  
Vol 92 (867) ◽  
pp. 376-378 ◽  
Author(s):  
Ann L. Phillips ◽  
Daniel J. McDonald
Keyword(s):  
Tribolium Castaneum ◽  
Wild Strain

scholarly journals Controlled Transcription of Regulator Gene carS by Tet-on or by a Strong Promoter Confirms Its Role as a Repressor of Carotenoid Biosynthesis in Fusarium fujikuroi

2020 ◽  
Vol 9 (1) ◽  
pp. 71
Author(s):  
Julia Marente ◽  
Javier Avalos ◽  
M. Carmen Limón
Keyword(s):  
Wild Strain ◽  
Ring Finger ◽  
Carotenoid Biosynthesis ◽  
Negative Regulator ◽  
Fusarium Fujikuroi ◽  
Structural Genes ◽  
Gene Encoding ◽  
In The Wild ◽  
Set Up

Carotenoid biosynthesis is a frequent trait in fungi. In the ascomycete Fusarium fujikuroi, the synthesis of the carboxylic xanthophyll neurosporaxanthin (NX) is stimulated by light. However, the mutants of the carS gene, encoding a protein of the RING finger family, accumulate large NX amounts regardless of illumination, indicating the role of CarS as a negative regulator. To confirm CarS function, we used the Tet-on system to control carS expression in this fungus. The system was first set up with a reporter mluc gene, which showed a positive correlation between the inducer doxycycline and luminescence. Once the system was improved, the carS gene was expressed using Tet-on in the wild strain and in a carS mutant. In both cases, increased carS transcription provoked a downregulation of the structural genes of the pathway and albino phenotypes even under light. Similarly, when the carS gene was constitutively overexpressed under the control of a gpdA promoter, total downregulation of the NX pathway was observed. The results confirmed the role of CarS as a repressor of carotenogenesis in F. fujikuroi and revealed that its expression must be regulated in the wild strain to allow appropriate NX biosynthesis in response to illumination.

scholarly journals Amikacin and bacteriophage treatment modulates outer membrane proteins composition in Proteus mirabilis biofilm

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Agnieszka Maszewska ◽  
Magdalena Moryl ◽  
Junli Wu ◽  
Bin Liu ◽  
Lu Feng ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Proteus Mirabilis ◽  
Protein Biosynthesis ◽  
Outer Membrane Proteins ◽  
Wild Strain ◽  
Elongation Factor ◽  
Resistance Mechanisms ◽  
Trna Synthetases ◽  
Cytoplasmic Proteins

AbstractModification of outer membrane proteins (OMPs) is the first line of Gram-negative bacteria defence against antimicrobials. Here we point to Proteus mirabilis OMPs and their role in antibiotic and phage resistance. Protein profiles of amikacin (AMKrsv), phage (Brsv) and amikacin/phage (AMK/Brsv) resistant variants of P. mirabilis were compared to that obtained for a wild strain. In resistant variants there were identified 14, 1, 5 overexpressed and 13, 5, 1 downregulated proteins for AMKrsv, Brsv and AMK/Brsv, respectively. Application of phages with amikacin led to reducing the number of up- and downregulated proteins compared to single antibiotic treatment. Proteins isolated in AMKrsv are involved in protein biosynthesis, transcription and signal transduction, which correspond to well-known mechanisms of bacteria resistance to aminoglycosides. In isolated OMPs several cytoplasmic proteins, important in antibiotic resistance, were identified, probably as a result of environmental stress, e.g. elongation factor Tu, asparaginyl-tRNA and aspartyl-tRNA synthetases. In Brsv there were identified: NusA and dynamin superfamily protein which could play a role in bacteriophage resistance. In the resistant variants proteins associated with resistance mechanisms occurring in biofilm, e.g. polyphosphate kinase, flagella basal body rod protein were detected. These results indicate proteins important in the development of P. mirabilis antibiofilm therapies.

scholarly journals Survival and mutant production induced by mutagenic agents in Metarhizium anisopliae

1995 ◽  
Vol 52 (3) ◽  
pp. 548-554 ◽  
Author(s):  
V. Kava - Cordeiro ◽  
E.A. Luna - Alves - Lima ◽  
J.L. Azevedo
Keyword(s):  
Gamma Radiation ◽  
Ultraviolet Light ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Nitrous Acid ◽  
Wild Strain ◽  
Wild Type ◽  
Survival Curves ◽  
Auxotrophic Mutants ◽  
Morphological Mutants

A wild strain of Metarhizium anisopliae, an entomopathogenic fungus, was submitted to three mutagenic agents: gamma radiation, ultraviolet light and nitrous acid. Survival curves were obtained and mutants were selected using different mutagenic doses which gave 1 to 5% survival. Morphological and auxotrophic mutants were isolated. Morphological mutants were grouped in a class with yellow conidia and other with pale vinaceous conidia as opposed to the green wild type conidia. Auxotrophic mutants had requirements for vitamin and aminoacid biosynthesis. More than 58% of the total auxotrophk mutants required proline/aipnine. Gamma radiation showed to be the most efficient mutagenic agent giving 0.2% of auxotrophk mutants followed by ultraviolet light (0.12%) and nitrous acid (0.06%).The conidial colour and auxotrophk mutants isolated until now from M. anisopliae were reviewed.

Characterization of a Wild Strain of Alicyclobacillus acidoterrestris: Heat Resistance and Implications for Tomato Juice

2011 ◽  
Vol 76 (2) ◽  
pp. M130-M136 ◽  
Author(s):  
Antonio Bevilacqua ◽  
Maria R. Corbo
Keyword(s):  
Heat Resistance ◽  
Wild Strain ◽  
Tomato Juice ◽  
Alicyclobacillus Acidoterrestris

STRAIN IMPROVEMENT OF A FUNGUS PRODUCING CHITINASE BY A CHEMICAL MUTAGEN

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (11) ◽  
pp. 25-28
Author(s):  
K Narayanan ◽  
◽  
N.D. Chopade ◽  
V.M Subrahmanyam ◽  
J. Venkata Rao
Keyword(s):  
Protein Content ◽  
Ethidium Bromide ◽  
Yeast Extract ◽  
Wild Strain ◽  
Strain Improvement ◽  
Cost Effective ◽  
Specific Protein ◽  
Chemical Mutagen ◽  
Yeast Extract Medium ◽  
Extract Medium

Microbial chitinases are commercially exploited for their biocontrol properties and generation of useful products from chitinous waste. Availability of highly active chitinolytic enzymes is a major problem. The present study was carried out to improve chitinase production by Aspergillus terreus using a chemical mutagen, ethidium bromide. The organism was cultivated on lactose- yeast extract medium. The production medium consisting of chitin- yeast extract medium was seeded at 10% level. The wild strains were exposed to ethidium bromide in the concentration range 1.5- 6.0 µg/mL. Generally, all the mutated strains showed an improved chitinase yield compared to the control. Highest yield was observed with the strain exposed to 6 µg/mL of ethidium bromide. The yield was 25.03 % higher compared to the wild strain. The mutated strain was slimy in nature. Protein content of the mutated strain decreased by 11%. Ethidium bromide at a concentration of 1.5 µg/mL was considered optional, at which the strain was stable with increase of 21.80 % in enzyme activity and 4.41% increase in protein content. Increased enzyme yield with decreased non-specific protein could be useful in producing cost effective enzyme.

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