Accelerated Phenol Removal by Amplifying the Metabolic Pathway with a Recombinant Plasmid Encoding Catechol 2, 3 Oxygenase

1992 ◽  
Vol 26 (9-11) ◽  
pp. 2191-2194 ◽  
Author(s):  
M. Fujita ◽  
M. Ike ◽  
T. Kamiya
Keyword(s):  
Metabolic Pathway ◽  
Recombinant Plasmid ◽  
Removal Rate ◽  
Phenol Degradation ◽  
Wild Strain ◽  
Phenol Concentration ◽  
Phenol Removal

The metabolic pathway of the phenol degradation in Pseudomonasputida BH was amplified by introducing the recombinant plasmid containing catechol 2,3 oxygenase gene isolated fron the chromosome of BH. This strain could degrade phenol and grow much faster than the wild strain at the phenol concentration of 100mg/L. This strain seems to accelerate the phenol removal rate if it is applied to the treatment of wastewater containing phenol.

scholarly journals Phenol Removal by a Novel Non-Photo-Dependent Semiconductor Catalyst in a Pilot-Scaled Study: Effects of Initial Phenol Concentration, Light, and Catalyst Loading

2014 ◽  
Vol 2014 ◽  
pp. 1-8
Author(s):  
Xiao Chen ◽  
Yan Liang ◽  
Xuefei Zhou ◽  
Yanling Zhang
Keyword(s):  
Titanium Dioxide ◽  
Uv Radiation ◽  
Phenol Degradation ◽  
Phenol Concentration ◽  
Catalyst Loading ◽  
Phenol Removal ◽  
Operational Parameters ◽  
Initial Concentration ◽  
Light Area ◽  
Initial Phenol Concentration

A novel non-photo-dependent semiconductor catalyst (CT) was employed to degrade phenol in the present pilot-scaled study. Effect of operational parameters such as phenol initial concentration, light area, and catalyst loading on phenol degradation, was compared between CT catalyst and the conventional photocatalyst titanium dioxide. CT catalyst excelled titanium dioxide in treating and mineralizing low-level phenol, under both mild UV radiation and thunder conditions of nonphoton. The result suggested that CT catalyst could be applied in circumstances when light is not easily accessible in pollutant-carrying media (e.g., particles, cloudy water, and colored water).

Effect of Conventional Carbon Sources on Phenol Degradation by Bacillus sp. CDQ

2013 ◽  
Vol 726-731 ◽  
pp. 301-304 ◽  
Author(s):  
Xi Pu He ◽  
Jie Liu ◽  
Hong Jie Liu ◽  
Sen Sheng Wang ◽  
Wen Hui Xu ◽  
...  
Keyword(s):  
Glucose Concentration ◽  
Sodium Acetate ◽  
Removal Rate ◽  
Phenol Degradation ◽  
Carbon Sources ◽  
Phenol Removal ◽  
Biodegradation Rate ◽  
Phenol Biodegradation ◽  
Noticeable Improvement

The influence on the growth and phenol biodegradation ofBacillussp. CDQ by three different conventional carbon sources were investigated. The results indicated that conventional carbon sources certainly affected the growth of strain CDQ and the biodegradation of phenol. Under the concentration of 1.5 to 3 g L-1, contrasting to the comparison, glucose improved the growth of theBacillussp. CDQ but inhibited the phenol biodegradation byBacillussp. CDQ. And the effect of inhibition increased with increasing glucose concentration. Below 1.5 g L-1, the rate of phenol removal increased with the amount of glucose added. Phenol biodegradation rate obviously decreased in the presence of sodium acetate. Lactose can significantly improve the rate of phenol biodegradation. However, no noticeable improvement on the removal rate of phenol was observed under different concentrations of lactose.

Biodegradation of High Phenol Concentration in a Membrane Bioreactor

2008 ◽  
Vol 6 (1) ◽  
Author(s):  
Benoît Marrot ◽  
Adrian Barrios-Martinez ◽  
Philippe Moulin ◽  
Nicolas Roche
Keyword(s):  
Activated Sludge ◽  
Removal Efficiency ◽  
Membrane Bioreactor ◽  
Substrate Inhibition ◽  
Phenol Degradation ◽  
Phenol Concentration ◽  
Phenol Removal ◽  
Permeate Flux ◽  
Phenol Biodegradation ◽  
Haldane Model

Phenol biodegradation by mixed culture was studied in a membrane bioreactor (MBR) over a period of 285 days. Activated sludge was used as the MBR biomass, after controlled acclimation to high phenol concentrations. The MBR permeate flux was stabilized quickly (in a few hours) and always maintained above 90 L.h-1.m-2.bar-1. The acclimatized activated sludge allowed significant phenol degradation (95% average COD removal efficiency and greater than 99% phenol removal efficiency) without supplemental reagent addition. After sludge acclimatization, the Haldane kinetics model for a single substrate was used to obtain the maximum specific growth rate (µm = 0.438 h-1), the half saturation coefficient (Ks = 29.54 mg.L-1) and the substrate inhibition constant (Ki = 72.45 mg.L-1). Biodegradation experiments were conducted at different phenol concentrations (4.9 – 8.5 g.L-1 d-1). Although the phenol concentration was high, the Haldane model was still acceptable, and removal capacities were in agreement with literature. Excellent effluent quality was obtained regardless of the extremely short SRT (5 – 17 days). This work shows the potential of MBR for toxic chemical elimination, charged effluents treatment and process stability.

Phenol Degradation in the System of H2O2/Porous Tourmaline Composite Materials

2010 ◽  
Vol 178 ◽  
pp. 196-201
Author(s):  
Can Li ◽  
Yan Ding ◽  
Jun Ping Meng ◽  
Li Fang Zhao
Keyword(s):  
Activation Energy ◽  
Hydrogen Peroxide ◽  
Removal Rate ◽  
Phenol Degradation ◽  
Good Effect ◽  
Phenol Removal ◽  
Reaction Conditions ◽  
Optimum Reaction ◽  
Initial Phenol Concentration ◽  
Reaction Activation Energy

Porous tourmaline composite material (PTCM) was prepared mainly by schorl and used to catalyze hydrogen peroxide for the removal of phenol. The optimum reaction conditions were determined by testing the phenol removal rate under the conditions of different initial phenol concentration, hydrogen peroxide dosage, PTCM dosage and temperature. The reaction activation energy was calculated to be 32.148KJ/mol, indicating that PTCM showed good effect on catalyzing hydrogen peroxide, phenol could be degraded quickly and the removal rate could reach 97%. The mechanism of the system was the Fenton-like reaction

scholarly journals Degradation Effect of UV Synergistic Biomass Activated Carbon Materials on Phenol Pollutants in Water

2021 ◽  
Vol 2079 (1) ◽  
pp. 012001
Author(s):  
Qi Zhang
Keyword(s):  
Activated Carbon ◽  
Influencing Factors ◽  
Removal Rate ◽  
Phenol Degradation ◽  
Polluted Water ◽  
Phenol Removal ◽  
Initial Concentration ◽  
Carbon Biomass ◽  
Degradation Effect ◽  
Degradation Ability

Abstract The purpose of the study is to purify the water containing phenol pollutants. The degradation effect of phenol pollutants in water is studied through the combined action of UV and biomass-activated carbon. First, the phenol solution is prepared in the laboratory to simulate the polluted water. Second, the phenol adsorption effects of UV synergistic biomass activated carbon, biomass activated carbon and ordinary industrial activated carbon under different influencing factors are compared by experiments. Finally, the results are analyzed and the conclusions are drawn. The results show that the UV synergistic biomass activated carbon has the strongest degradation ability for phenol, and the highest removal rate is 66.5% when the shaking time is 65 minutes. The adsorption ability of the industrial activated carbon for phenol is the worst. When the initial concentration of phenol is 25mg/L, the maximum phenol removal rate is 96.8%. The maximum phenol removal rate of biomass activa ted carbon appears in the initial concentration of phenol and the phenol removal rate is 60 mg/L. The reaction temperature has little effect on the phenol removal rate of UV synergistic biomass activated carbon and biomass activated carbon. The phenol removal ability of UV synergistic biomass activated carbon and biomass activated carbon reaches the highest when the dosage of activated carbon is 2.0 g, and the rates are 96.4% and 91.1%, respectively. When the pH of the solution is 7, the removal rate of UV synergistic biomass activated carbon reaches a maximum of 97%. When the pH of the solution is 6, the removal rate of biomass-activated carbon reaches the maximum. When the pH of the ordinary industrial activated carbon is 7, the removal rate is the maximum. Due to different influencing factors, UV synergistic biomass activated carbon has the strongest phenol degradation ability. This study provides a reference for the purification of polluted water.

scholarly journals Application of Pier Waste Sludge for Catalytic Activation of Proxy-monosulfate and Phenol Elimination From a Petrochemical Wastewater

2021 ◽  
Author(s):  
Feyzollah Khoshtinat ◽  
Tayebeh Tabatabaie ◽  
Bahman Ramavandi ◽  
Seyedenayat Hashemi
Keyword(s):  
Removal Rate ◽  
Chemical Properties ◽  
Phenol Concentration ◽  
Catalyst Stability ◽  
Phenol Removal ◽  
Operational Parameters ◽  
Petrochemical Wastewater ◽  
Waste Sludge ◽  
Before And After ◽  
Phenol Decomposition

Abstract This investigation aimed to remove phenol from a real wastewater (taken from a petrochemical company) by activating peroxy-monosulfate (PMS) using catalysts extracted from pier waste sludge. The physical and chemical properties of the catalyst were evaluated by FE-SEM/EDS, XRD, FTIR, and TGA/DTG tests. The functional groups of O-H, C-H, CO32-, C-H, C-O, N-H, and C-N were identified on the catalyst surface. Also, the crystallinity of the catalyst before and after reaction with petrochemical wastewater was 103.4 nm and 55.8 nm, respectively. Operational parameters of pH (3-9), catalyst dose (0-100 mg/L), phenol concentration (50-250 mg/L), and PMS concentration (0-250 mg/L) were tested to remove phenol. The highest phenol removal rate (94%) was obtained at pH=3, catalyst dose of 80 mg/L, phenol concentration of 50 mg/L, PMS concentration of 150 mg/L, and contact time of 150 min. Phenol decomposition in petrochemical wastewater followed the first-order kinetics (k> 0.008 min-1, R2> 0.94). Based on the reported results, it was found that the pH factor is more important than other factors in phenol removal. The catalyst stability test was performed for up to five cycles and phenol removal in the fifth cycle was reduced to 42%. Also, the energy consumption in this study was 77.69 kw.h/m3. According to the results, the pier waste sludge catalyst/PMS system is a critical process for eliminating phenol from petrochemical wastewater.

scholarly journals Advanced Oxidation Processes (AOPs) for Refinery Wastewater Treatment Contains High Phenol Concentration

2018 ◽  
Vol 156 ◽  
pp. 03012 ◽  
Author(s):  
Alif Nurul Azizah ◽  
I Nyoman Widiasa
Keyword(s):  
Advanced Oxidation Processes ◽  
Petroleum Refinery ◽  
Phenol Degradation ◽  
Phenol Concentration ◽  
Phenol Removal ◽  
Biological Processes ◽  
Refinery Wastewater ◽  
Treated Effluent ◽  
Petroleum Refinery Wastewater ◽  
High Phenol Concentration

Petroleum Refinery wastewater is characterized by a high phenol content. Phenol is toxic and resistant to biological processes for treatment of the petroleum refinery wastewater. The combination of an AOP and a biological process can be used for treatment of the refinery wastewater. It is necessary to conduct a study to determine the appropriate condition of AOP to meet the phenol removal level. Two AOP configurations were investigated: H2O2 / UV and H2O2 / UV / O3. From each process samples, COD, phenol and pH were measured. The oxidation was carried out until the targeted phenol concentration of treated effluent were obtained. The better result obtained by using process H2O2 / UV / O3 with the H2O2 concentration 1000 ppm. After 120 minutes, the final target has been achieved in which phenol concentration of 37.5 mg/L or phenol degradation of 93.75%.

Shear Loss Characteristics of an Aerobic Biofilm

1992 ◽  
Vol 26 (3-4) ◽  
pp. 595-600 ◽  
Author(s):  
S. M. Rao Bhamidimarri ◽  
T. T. See
Keyword(s):  
Growth Rate ◽  
Shear Stress ◽  
Film Thickness ◽  
Removal Rate ◽  
Surface Growth ◽  
Phenol Removal ◽  
Concentric Cylinder ◽  
Substrate Utilisation ◽  
Utilisation Rate ◽  
Net Growth Rate

Growth and shear loss characteristics of phenol utilizing biofilm were studied in a concentric cylinder bioreactor. The net accumulation of the biofilm and the substrate utilisation were measured as a function of torque. Uniform biofilms were obtained up to a thickness of around 300 microns, beyond which the surface growth was non-uniform. The substrate utilisation rate, however, reached a constant value beyond film thickness of 50 to 100 microns depending on the operational torque. The maximum phenol removal rate was achieved at a shear stress of 3.5 Nm-2. The effect of shear stress on net growth rate was found to be described byand a zero net growth was obtained at a shear stress of 18.7 Nm-2.

Phenol Removal from Contaminated Wastewater Using Activated Carbon/Zeolite Composite Coated with Titanium Dioxide

2016 ◽  
Vol 690 ◽  
pp. 103-108
Author(s):  
Khemmakorn Gomonsirisuk ◽  
Thanakorn Wasanapiarnpong ◽  
Charusporn Mongkolkachit
Keyword(s):  
Titanium Dioxide ◽  
Activated Carbon ◽  
High Efficiency ◽  
Adsorption Property ◽  
Phenol Concentration ◽  
Study Phase ◽  
Phenol Removal ◽  
High Porosity ◽  
Good Efficiency ◽  
Composite Substrate

Phenol and phenolic compounds in wastewater from various industries were toxic to water livings and human even in ppm concentration. A number of photocatalysts and adsorbents were applied for the low cost and good efficiency wastewater management to reduce phenol concentration in water. In this work titanium dioxide, one of high efficiency photocatalysts which is widely used in water treatement, was coated on the fabricated adsorbent composite substrate. The composite substrate composed of activated carbon and NaA zeolite presents high phenol adsorption because of high porosity and good ion exchange properties resulting in good adsorption property. Accordingly, the absorption could promote the photocatalytic activity of TiO2 catalyst. As the specimens were easily disposed after water treatment process, therefore, it was a good choice for lower energy consumption. The composite substrate was easily fabricated by simple extrusion and fired under non oxidation atmosphere at 650°C for 3 hours. Then polyurethane foam was inserted into the composite substrate to make it be able to float and be swirled by wind near water surface to get more UV excitation than deeper water. Phenol concentration was investigated by the UV absorbance at 270 nm using UV-Vis spectrophotometer. The XRD and SEM were used to study phase crystal structure and morphology of the composite.

Removal of high concentration phenol from aqueous solutions by electrochemical technique

2021 ◽  
Vol 39 (2A) ◽  
pp. 189-195
Author(s):  
Shaimaa T. Alnasrawy ◽  
Ghayda Y. Alkindi ◽  
Taleb M. Albayati
Keyword(s):  
Current Density ◽  
Electrochemical Cell ◽  
Removal Rate ◽  
Electrochemical Process ◽  
Phenol Concentration ◽  
Electrochemical Technique ◽  
Process Conditions ◽  
Electrolysis Time ◽  
High Concentration ◽  
Maximum Removal

In this study, the ability of the electrochemical process to remove aqueous high concentration phenol using an electrochemical cell with aluminum anode and cathode was examined. The removal rate of phenol was monitored using different parameters phenol concentration, pH, electrolysis time, current density, and electrode distance. Obtained results indicated that the low removal rates of phenol were observed at both low and high pH. However, the removal rate of phenol increased with an increase in the current density, each electrochemical process conditions need a certain electrodes distance. removal rate of phenol decreased with the increase in the initial phenol concentration. The maximum removal rate of phenol obtained from this study was 82%.

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