Optimization of arabinogalactan and taxifolin extraction process from Dahurian larch (Larix gmelinii ) and evaluation of the effects on activities of α-amylase, α-glycosidase, and pancreatic lipase in vitro

2018 ◽  
Vol 42 (5) ◽  
pp. e12607 ◽  
Author(s):  
Zaizhi Liu ◽  
Mengxia Wei ◽  
Guoqiang Cui ◽  
Xinyu Yang ◽  
Huiyan Gu ◽  
...  
Keyword(s):  
Pancreatic Lipase ◽  
Extraction Process ◽  
Larix Gmelinii ◽  
Dahurian Larch

scholarly journals Optimization of sunflower head pectin extraction by ammonium oxalate and the effect of drying conditions on properties

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xuemei Ma ◽  
Jiayi Yu ◽  
Jing Jing ◽  
Qian Zhao ◽  
Liyong Ren ◽  
...  
Keyword(s):  
High Performance ◽  
Freeze Drying ◽  
Antioxidant Activities ◽  
Structural Characteristics ◽  
Radical Scavenging ◽  
Ammonium Oxalate ◽  
Extraction Process ◽  
Size Exclusion ◽  
Pharmaceutical Industries

AbstractPectin is a kind of natural and complex carbohydrates which is extensively used in food, chemical, cosmetic, and pharmaceutical industries. Fresh sunflower (Helianthus annuus L.) heads were utilized as a novel source of pectin extracted by ammonium oxalate. The conditions of the extraction process were optimized implementing the response surface methodology. Under optimal extraction parameters (extraction time 1.34 h, liquid–solid ratio 15:1 mL/g, ammonium oxalate concentration 0.76% (w/v)), the maximum experimental yield was 7.36%. The effect of spray-drying and freeze-drying on the physiochemical properties, structural characteristics, and antioxidant activities was investigated by FT-IR spectroscopy, high performance size exclusion chromatography, and X-ray diffraction. The results showed freeze-drying lead to decrease in galacturonic acid (GalA) content (76.2%), molecular weight (Mw 316 kDa), and crystallinity. The antioxidant activities of pectin were investigated utilizing the in-vitro DPPH and ABTS radical-scavenging systems. This study provided a novel and efficient extraction method of sunflower pectin, and confirmed that different drying processes had an effect on the structure and properties of pectin.

scholarly journals Inhibition of pancreatic lipase in vitro by the covalent inhibitor tetrahydrolipstatin

1988 ◽  
Vol 256 (2) ◽  
pp. 357-361 ◽  
Author(s):  
P Hadváry ◽  
H Lengsfeld ◽  
H Wolfer
Keyword(s):  
Transition State ◽  
Acid Derivative ◽  
Pancreatic Lipase ◽  
Active Site ◽  
Boronic Acid ◽  
Selective Inhibitor ◽  
Covalent Intermediate ◽  
State Form ◽  
Covalent Inhibitor

Tetrahydrolipstatin inhibits pancreatic lipase from several species, including man, with comparable potency. The lipase is progressively inactivated through the formation of a long-lived covalent intermediate, probably with a 1:1 stoichiometry. The lipase substrate triolein and also a boronic acid derivative, which is presumed to be a transition-state-form inhibitor, retard the rate of inactivation. Therefore, in all probability, tetrahydrolipstatin reacts with pancreatic lipase at, or near, the substrate binding or active site. Tetrahydrolipstatin is a selective inhibitor of lipase; other hydrolases tested were at least a thousand times less potently inhibited.

scholarly journals In vitro Antidiabetic, anti-obesity and antioxidant proprities of Rosemary extracts

2014 ◽  
Vol 10 (2) ◽  
pp. 2305-2316 ◽  
Author(s):  
Manel Ben Ali ◽  
Kais Mnafgui ◽  
Abdelfattah Feki ◽  
Mohamed Damak ◽  
Noureddine Allouche
Keyword(s):  
Essential Oil ◽  
Ethyl Acetate ◽  
Pancreatic Lipase ◽  
Antioxidant Properties ◽  
Methanolic Extracts ◽  
Ic50 Values ◽  
Inhibitory Potential ◽  
Prevention Of Diabetes ◽  
First Time

Diabetes mellitus is a serious health problem worldwide that has adverse and long-lasting consequences for individuals, families, and communities. Hence, this study sought to investigate the inhibitory potential of rosemary extracts on key-enzymes related to diabetes such as α-amylase and pancreatic lipase activities, as well as to assess their antioxidant properties in vitro. The IC50 values of Rosemary essential oil, ethyl acetate and methanolic extracts against α-amylase were 28.36, 34.11 and 30.39 µg/mL respectively, and those against pancreatic lipase were 32.25, 36.64 and 34.07 µg/mL, suggesting strong anti-diabetic and anti-obesity effects of Rosemary. The methanolic extract was found to be the highest in levels of phenolic (282.98 µgGAE/mg extract) and flavonoids (161.05 µg QE /mg extract) contents as well as in the antioxidant activity (IC50 = 15.82 µg/mL) as compared to other extracts ethyl acetate (IC50 = 32.23 µg/mL) and essential oil  (IC50 = 96.12 µg/mL).Antioxidant efficacy of Rosemary extracts has been estimated in the stabilization of sunflower oil (SFO) at three different concentrations, i.e. 200 (SFO-200), 500 (SFO-500) and 1000 ppm (SFO-1000). Results showed the highest efficiency of SFO-1000.The results obtained in this study demonstrated for the first time that Rosemary is a potent source of natural inhibitors of α-amylase and pancreatic lipase with powerful antioxidants proprieties that might be used in the food stabilization and the prevention of diabetes and obesity complications as a complementary pharmacological drug.

scholarly journals Phytochemical analysis, Antimicrobial, insecticidal and antiradical activity of Hydnocarpus pentandra (Buch.-Ham.) Oken

2017 ◽  
Vol 9 (4) ◽  
pp. 576
Author(s):  
Prashith Kekuda TR ◽  
Dunkana Negussa Kenie ◽  
Chetan DM ◽  
Raghavendra L Hallur
Keyword(s):  
Leaf Extract ◽  
Antiradical Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Extraction Process ◽  
In Vitro Assays ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Bioactive Agents

<p><strong>Objectives</strong>: The present study was conducted to evaluate antimicrobial, insecticidal and radical scavenging activity of leaf extract of <em>Hydnocarpus pentandra</em> (Buch.-Ham.) Oken belonging to the family Achariaceae.</p><p><strong>Methods</strong>: Extraction process of shade dried and powdered leaf was carried out by maceration technique. Extract was screened for phytochemicals by standard tests. Antibacterial and antifungal activity of leaf extract was determined by Agar well diffusion and Poisoned food technique respectively. Antiradical activity of leaf extract was evaluated by two in vitro assays namely 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS) free radical scavenging assays. Insecticidal activity of leaf extract was determined against II instar and IV instar larvae of <em>Aedes aegypti</em>.</p><p><strong>Results</strong>: Preliminary phytochemical analysis showed the presence of alkaloids, flavonoids, tannins, saponins, glycosides, triterpenes and steroids in the leaf extract. Leaf extract exhibited marked inhibitory activity against Gram positive bacteria when compared to Gram negative bacteria. <em>Bacillus cereus</em> (zone of inhibition 1.86±0.05cm) and <em>Escherichia coli</em> (zone of inhibition 1.06±0.05cm) were inhibited to highest and least extent respectively. Extract was effective in inhibiting mycelial growth of seed-borne fungi. Among fungi, the susceptibility to extract was in the order: <em>Curvularia</em> sp. (53.64% inhibition) &gt; <em>Fusarium</em> sp. (45.81% inhibition) &gt; <em>Alternaria</em> sp. (35.08% inhibition). The extract exhibited concentration dependent larvicidal activity with marked activity being observed against II instar larvae (LC<sub>50</sub> value 0.79mg/ml) when compared to IV instar larvae (LC<sub>50</sub> value 1.37mg/ml). Leaf extract scavenged DPPH and ABTS radicals dose dependently with an IC<sub>50</sub> value of 13.91µg/ml and 6.03µg/ml respectively.</p><p><strong>Conclusions</strong>: The plant is shown to be an important source of bioactive agents. The observed bioactivities could be attributed to the phytochemicals present in the leaf extract. Further studies on characterization and bioactivity determination of isolated components from leaf extract are to be carried out.</p>

scholarly journals In Vitro Pancreatic Lipase Inhibition by Marine Fungi Purpureocillium lilacinum Associated with Stylissa sp. Sponge as Anti-obesity Agent

2021 ◽  
Vol 29 (1) ◽  
pp. 76-86
Author(s):  
Wendi Nurul Fadillah ◽  
Nampiah Sukarno ◽  
Dyah Iswantini ◽  
Min Rahminiwati ◽  
Novriyandi Hanif ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Pancreatic Lipase ◽  
Metabolite Identification ◽  
Molecular Characteristics ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Purpureocillium Lilacinum ◽  
Pancreatic Lipase Inhibition ◽  
Lipase Inhibition

This study aimed to evaluate the potential of marine fungus Purpureocillium lilacinum isolated from an Indonesian marine sponge Stylissa sp. as an anti-obesity agent through pancreatic lipase inhibition assay. The fungus was identified as P. lilacinum through morphological and molecular characteristics. The fungal extract’s inhibition activity and kinetics were evaluated using spectrophotometry and Lineweaver-Burk plots. Ethyl acetate and butanol were used for extraction. Both extracts showed pancreatic lipase inhibition in a concentration-dependent manner. Both crude extracts were then fractionated once. All fractionated extracts showed inhibitory activity above 50%, with the highest activity found in fraction 5 of ethyl acetate at 93.41% inhibition. The best fractionated extract had an IC50value of 220.60 µg.mL-1. The most active fraction of P. lilacinum had a competitive-type inhibitor behavior as shown by the value of Vmax not significantly changing from 388.80 to 382.62 mM pNP.min-1, and the Michaelis-Menten constant (KM) increased from 2.02 to 5.47 mM in the presence of 500 µg.mL-1 fractionated extract. Metabolite identification with LC-MS/MS QTOF suggested that galangin, kaempferol, and quercetin were responsible for the observed lipase inhibition.

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