Chlorogenic acid improves lipid membrane peroxidation and morphological changes in nitrite‐induced erythrocyte model of methemoglobinemia

2020 ◽  
Vol 44 (5) ◽  
Author(s):  
Dai Cheng ◽  
Guangliang Wang ◽  
Xuerui Wang ◽  
Jinlei Tang ◽  
Chao Li
Keyword(s):  
Chlorogenic Acid ◽  
Lipid Membrane ◽  
Morphological Changes ◽  
Membrane Peroxidation

Cytokines stimulate lipid membrane peroxidation of human sperm **Supported in part by a grant from the University of Connecticut Health Center, Farmington, Connecticut.

1994 ◽  
Vol 62 (1) ◽  
pp. 186-188 ◽  
Author(s):  
Jeffrey P. Buch ◽  
Thomas F. Kolon ◽  
Nilanjana Maulik ◽  
Donald L. Kreutzer ◽  
Dipak K. Das
Keyword(s):  
Health Center ◽  
Lipid Membrane ◽  
Human Sperm ◽  
The University ◽  
University Of Connecticut ◽  
Membrane Peroxidation

Cell Cycle Arrest and Apoptosis Induction of Phloroacetophenone Glycosides and Caffeoylquinic Acid Derivatives in Gastric Adenocarcinoma (AGS) Cells

2018 ◽  
Vol 18 (4) ◽  
pp. 610-616 ◽  
Author(s):  
Naser Jafari ◽  
Seyed Jalal Zargar ◽  
Mohammad-Reza Delnavazi ◽  
Narguess Yassa
Keyword(s):  
Cell Cycle ◽  
Cell Death ◽  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Cancer Cells ◽  
Morphological Changes ◽  
G1 Phase ◽  
Mrna Levels ◽  
Apoptosis Induction ◽  
Caffeoylquinic Acid

Introduction: In the present study, we analyzed anti-proliferative and apoptosis induction activity of five phenolic compounds: echisoside, pleoside, chlorogenic acid, 4,5-Di-O-caffeoylquinic acid, and cynarin on AGS (adenocarcinoma gastric) cell line. Method: These phenolic compounds were isolated from methanol extract of Dorema glabrum root. An MTT assay was conducted to evaluate the inhibitory effect on cancer cells. EB/AO staining was done to assess the mode of cell death and morphological changes of the cells’ nuclei. Cell cycle distribution of the cells was analyzed by flow cytometry, and for further confirmation of the pathway, mRNA levels of apoptosis cascade players were quantified by qRT-PCR. Result: We found that echisoside, pleoside, chlorogenic acid, 4,5-Di-O-caffeoylquinic acid, and cynarin inhibited the proliferation of AGS cancer cells in vitro. Our data revealed that these compounds triggered morphological changes characteristic of apoptotic cell death. These compounds up-regulated bax and caspase3 expression and down-regulated cyclin D1, bcl2, VEGFA, c-myc and survivin. Moreover, cell population increased at the G1 phase, and a number of cells at the G2/M phase of the cell cycle decreased after treatment. Conclusion: All these data suggest that phenolic compounds have a cytotoxic effect on gastric cancer cells and could trigger apoptosis. Besides cytotoxic activity, they could potentially arrest the cell cycle at the G1 phase.

scholarly journals Improving the Recovery of Phenolic Compounds from Spent Coffee Grounds (SCG) by Environmentally Friendly Extraction Techniques

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 613
Author(s):  
Ilhami Okur ◽  
Betul Soyler ◽  
Purlen Sezer ◽  
Mecit Halil Oztop ◽  
Hami Alpas
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
High Performance ◽  
Morphological Changes ◽  
Total Phenolic ◽  
Extraction Techniques ◽  
Spent Coffee Grounds ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Coffee Grounds

The aim of this study was to investigate and compare the effects of different extraction techniques (high hydrostatic pressure-assisted extraction (HHPE), ultrasound-assisted extraction (UAE), and classical solvent extraction (CSE)) on phenolic compounds from spent coffee grounds (SCG). Different HHPE parameters (300, 400 and 500 MPa at 25 °C for 5, 10 and 15 min) and UAE parameters (40%, 50%, and 60% amplitude at 25 °C for 5, 10 and 15 min) were used. These techniques were compared with CSE (at 50 °C for 30 min) according to total phenolic content (TPC), antioxidant activity (AA), high-performance liquid chromatography (HPLC), scanning electron microscopy (SEM), and infrared (IR) spectroscopy. The results showed that eco-friendly techniques increased the TPC and AA compared to CSE and morphological changes were verified by SEM results. Furthermore, chlorogenic and caffeic acid were also quantified by using HPLC. Chlorogenic acid was found as the main phenolic compound in spent coffee grounds (SCG). The highest chlorogenic acid was detected as 85.0 ± 0.6 mg/kg FW with UAE at 60% amplitude for 15 min. In brief, for the extraction of phenolic compounds from waste SCG eco-friendly techniques such as HHPE and/or UAE were more convenient than CSE.

Effect of Exogenous Nitric Oxide Donor on Lipid Peroxidation and Antioxidant System in Needles of Taxus chinensis Var. mairei Seeding

2011 ◽  
Vol 183-185 ◽  
pp. 427-431
Author(s):  
De Wen Li ◽  
Xiao Xue Wei ◽  
Jing Hua Yu ◽  
Yuan Gang Zu
Keyword(s):  
Nitric Oxide ◽  
Lipid Membrane ◽  
Oxygen Metabolism ◽  
Nitric Oxide Donor ◽  
Test Material ◽  
Taxus Chinensis ◽  
Protective Enzyme ◽  
Active Oxygen Metabolism ◽  
Exogenous Nitric Oxide ◽  
Membrane Peroxidation

Taxus chinensis var. mairei was used as test material, exogenous nitric oxide (NO) fumigation treatment with different concentration (0, 0.01, 0.1, 0.5 or 1 mM) of sodium nitrop russide (SNP), a donor for exogenous NO, was used to study the effects of NO on the active oxygen metabolism index of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD), Hydrogen peroxide (H2O2) and malondialdehyde (MDA) in Taxus chinensis var. mairei. The results showed that low concentration of exogenous NO treatment increased the activities of the protective enzyme, such as SOD, CAT, POD and APX of Taxus chinensis var. mairei leaves, reduced the content of H2O2, and inhibit the increase of MDA in the first three days. NO fumigation treatment enhanced the antioxidant ability of Taxus chinensis var. mairei, prevented lipid membrane peroxidation.

scholarly journals Identification of Oxidative Stress in Red Blood Cells with Nanoscale Chemical Resolution by Infrared Nanospectroscopy

2018 ◽  
Vol 19 (9) ◽  
pp. 2582 ◽  
Author(s):  
Francesco Ruggeri ◽  
Curtis Marcott ◽  
Simone Dinarelli ◽  
Giovanni Longo ◽  
Marco Girasole ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Morphological Changes ◽  
Membrane Skeleton ◽  
Force Microscopy ◽  
Atomic Force ◽  
Membrane Peroxidation

During their lifespan, Red blood cells (RBC), due to their inability to self-replicate, undergo an ageing degradation phenomenon. This pathway, both in vitro and in vivo, consists of a series of chemical and morphological modifications, which include deviation from the biconcave cellular shape, oxidative stress, membrane peroxidation, lipid content decrease and uncoupling of the membrane-skeleton from the lipid bilayer. Here, we use the capabilities of atomic force microscopy based infrared nanospectroscopy (AFM-IR) to study and correlate, with nanoscale resolution, the morphological and chemical modifications that occur during the natural degradation of RBCs at the subcellular level. By using the tip of an AFM to detect the photothermal expansion of RBCs, it is possible to obtain nearly two orders of magnitude higher spatial resolution IR spectra, and absorbance images than can be obtained on diffraction-limited commercial Fourier-transform Infrared (FT-IR) microscopes. Using this approach, we demonstrate that we can identify localized sites of oxidative stress and membrane peroxidation on individual RBC, before the occurrence of neat morphological changes in the cellular shape.

Evaluation of lipid membrane peroxidation, mitochondrial membrane potential and oxidative stress on bull semen frozen with reduced sperm number after colloidal single layer centrifugation

2018 ◽  
Vol 194 ◽  
pp. e25-e26
Author(s):  
Tairini Erica da Cruz ◽  
Alicio Martins ◽  
Fernanda Nunes Marqui ◽  
Tatiana Issa Uherara Berton ◽  
Camila de Paula Freitas-Dell’Aqua ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Lipid Membrane ◽  
Single Layer ◽  
Sperm Number ◽  
Bull Semen ◽  
Membrane Peroxidation ◽  
And Oxidative Stress

Effects of salt treatment on growth, lipid membrane peroxidation, polyphenol content, and antioxidant activities in leaves of Sesuvium portulacastrum L.

2017 ◽  
Vol 31 (4) ◽  
pp. 404-417 ◽  
Author(s):  
Inès Slama ◽  
Rawya M’Rabet ◽  
Riadh Ksouri ◽  
Ons Talbi ◽  
Ahmed Debez ◽  
...  
Keyword(s):  
Lipid Membrane ◽  
Antioxidant Activities ◽  
Sesuvium Portulacastrum ◽  
Salt Treatment ◽  
Polyphenol Content ◽  
Membrane Peroxidation
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