Remedial role of exercise training to deep‐fried oil‐induced metabolic and histological changes in Wistar rats

2020 ◽  
Vol 44 (11) ◽  
Author(s):  
Md. Abu Rayhan ◽  
Md Kamrul Islam ◽  
Mst. Afifa Khatun ◽  
Dipa Islam ◽  
Md. Nazibur Rahman
Keyword(s):  
Exercise Training ◽  
Wistar Rats ◽  
Histological Changes ◽  
Fried Oil

scholarly journals Effect of Silymarin Supplementation in Lung and Liver Histological Modifications during Exercise Training in a Rodent Model

2021 ◽  
Vol 6 (3) ◽  
pp. 72
Author(s):  
Nancy Vargas-Mendoza ◽  
Marcelo Angeles-Valencia ◽  
Ángel Morales-González ◽  
Mauricio Morales-Martínez ◽  
Eduardo Madrigal-Bujaidar ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Exercise Training ◽  
Lipid Content ◽  
Wistar Rats ◽  
Muscle Size ◽  
Cell Recovery ◽  
Pulmonary Tissue ◽  
Histological Changes ◽  
Male Wistar Rats ◽  
Intrahepatic Lipid

Background: Exercise training induces adaptive physiological and morphological modifications in the entire organism; however, excessive loads of training may increase damage in tissues. The purpose of this study was to evaluate the effect of silymarin in lung and liver histological changes in rats subjected to exercise training (ET). Methods: Male Wistar rats were subjected to an 8-week ET treadmill program 5 days per week, 60 min/session, and were previously administered 100 mg ascorbic acid or 100 mg of silymarin. Results: Silymarin increased alveolar and bronchial muscle size, improve vascularization, and reduced tissue inflammation. In liver, silymarin promoted the reduction of lipid content. Conclusion: Silymarin supplementation may improve inflammation in pulmonary tissue after 8 weeks of the ET treadmill program, improve cell recovery, and reduce intrahepatic lipid content.

scholarly journals High-volume endurance exercise training stimulates hematopoiesis by increasing ACE NH2-terminal activity

2021 ◽  
Author(s):  
Flávio de Castro Magalhães ◽  
Tiago Fernandes ◽  
Vinícius Bassaneze ◽  
Katt Coelho Mattos ◽  
Isolmar Schettert ◽  
...  
Keyword(s):  
Exercise Training ◽  
Endurance Exercise ◽  
Wistar Rats ◽  
High Volume ◽  
Erythroid Progenitor ◽  
Hematopoietic Stem ◽  
Endurance Exercise Training ◽  
First Time ◽  
Captopril Treatment

One of the health benefits of endurance exercise training (ET) is the stimulation of hematopoiesis. However, the mechanisms underlying ET-induced hematopoietic adaptations are understudied. N-Acetyl-Seryl-Aspartyl-Lysyl-Proline (Ac-SDKP) inhibits proliferation of early hematopoietic progenitor cells. The angiotensin-converting enzyme (ACE) NH2-terminal promotes hematopoiesis by inhibiting the anti-hematopoietic effect of Ac-SDKP. Here we demonstrate for the first time the role of ACE NH2-terminal in ET-induced hematopoietic adaptations. Wistar rats were subjected to 10 weeks of moderate-(T1) and high-(T2) volume swimming-training. Although both protocols induced classical ET-associated adaptations, only T2 increased plasma ACE NH2-domain activity (by 40%, p=0.0003) and reduced Ac-SDKP levels (by 50%, p<0.0001). T2 increased the number of hematopoietic stem cells (~200%, p=0.0008), early erythroid progenitor colonies (~300%, p<0.0001) and reticulocytes (~500%, p=0.0007), and reduced erythrocyte lifespan (~50%, p=0.022). Following, Wistar rats were subjected to T2 or T2 combined with ACE NH2-terminal inhibition (captopril treatment: 10 mg.kg-1.d-1). T2 combined with ACE NH2-terminal inhibition prevented Ac-SDKP decrease and attenuated ET-induced hematopoietic adaptations. Altogether, our findings show that ET-induced hematopoiesis was at least partially associated to increased ACE NH2-terminal activity and reduction of the hematopoietic inhibitor Ac-SDKP.

Effects of exercise training on papain-induced pulmonary emphysema in Wistar rats

2006 ◽  
Vol 100 (1) ◽  
pp. 281-285 ◽  
Author(s):  
Claudia Fló ◽  
Fernanda D. T. Q. S. Lopes ◽  
David I. Kasahara ◽  
Anna Cecília D. Silva ◽  
Rita C. C. Jesus ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Exercise Training ◽  
Respiratory System ◽  
Wistar Rats ◽  
Pulmonary Tissue ◽  
Exercise Time ◽  
Mean Linear Intercept ◽  
Respiratory System Resistance ◽  
Connective Tissue Remodeling

The purpose of the present study was to evaluate the role of exercise training on the development of papain-induced emphysema in rats. Our hypothesis was that the increase in pulmonary tissue stretching associated with exercise could increase the severity of a protease-induced emphysema. Wistar rats were randomly assigned to four groups ( n = 10 for each group) that received, respectively, intratracheal infusion of papain (6 mg in 1 ml of 0.9% NaCl) or vehicle and were submitted or not to a protocol of exercise on a treadmill. Rats exercised at 13.3 m/min, 6 days/wk, for 9 wk (increasing exercise time, from 10 to 35 min). We measured respiratory system elastance and resistance, the size and weight of the heart, and pulmonary mean linear intercept (Lm). After 9 wk of exercise training, there were no differences in respiratory system resistance and elastance values among the four experimental groups. Volume of the heart was significantly greater in rats submitted to exercise training ( P = 0.007) compared with sedentary rats due to increases in volumes of both right and left cardiac chambers. Lm was significantly greater in rats that received papain compared with saline-infused rats ( P = 0.025). Surprisingly, this was true, even though there was no significant decrease in elastance, possibly due to connective tissue remodeling. However, Lm was significantly greater in papain + exercise rats compared with rats that received papain and were not submitted to exercise. We conclude that exercise training can increase alveolar damage induced by papain infusion.

Role of cytokines in anti-implantation activity of H2receptor blockers in albino wistar rats

2009 ◽  
Vol 00 (00) ◽  
pp. 090930024626018-7
Author(s):  
Shyam S. Agrawal ◽  
Sibi P. Ittiyavirah
Keyword(s):  
Wistar Rats

scholarly journals Helminth Interactions with Bacteria in the Host Gut Are Essential for Its Immunomodulatory Effect

2021 ◽  
Vol 9 (2) ◽  
pp. 226
Author(s):  
Milan Jirků ◽  
Zuzana Lhotská ◽  
Lucia Frgelecová ◽  
Oldřiška Kadlecová ◽  
Klára Judita Petrželková ◽  
...  
Keyword(s):  
Protective Effect ◽  
Intestinal Inflammation ◽  
Hymenolepis Diminuta ◽  
Clinical Parameters ◽  
Th2 Response ◽  
Histological Changes ◽  
Immunological Markers ◽  
Bacterial Microbiota ◽  
Inflammatory Effect

Colonization by the benign tapeworm, Hymenolepis diminuta, has been associated with a reduction in intestinal inflammation and changes in bacterial microbiota. However, the role of microbiota in the tapeworm anti-inflammatory effect is not yet clear, and the aim of this study was to determine whether disruption of the microflora during worm colonization can affect the course of intestinal inflammation. We added a phase for disrupting the intestinal microbiota using antibiotics to the experimental design for which we previously demonstrated the protective effect of H. diminuta. We monitored the immunological markers, clinical parameters, bacterial microbiota, and histological changes in the colon of rats. After a combination of colonization, antibiotics, and colitis induction, we had four differently affected experimental groups. We observed a different course of the immune response in each group, but no protective effect was found. Rats treated with colonization and antibiotics showed a strong induction of the Th2 response as well as a significant change in microbial diversity. The microbial results also revealed differences in the richness and abundance of some bacterial taxa, influenced by various factors. Our data suggest that interactions between the tapeworm and bacteria may have a major impact on its protective effect.

scholarly journals The Role of High Fat Diets and Liver Peptidase Activity in the Development of Obesity and Insulin Resistance in Wistar Rats

Nutrients ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 636
Author(s):  
Germán Domínguez-Vías ◽  
Ana Belén Segarra ◽  
Manuel Ramírez-Sánchez ◽  
Isabel Prieto
Keyword(s):  
Glucose Metabolism ◽  
Wistar Rats ◽  
Metabolic Disorders ◽  
Peptidase Activity ◽  
High Fat ◽  
Β Cell ◽  
High Fat Diets ◽  
Glutamyl Transferase ◽  
Fat Diets

High-fat diets (HFD) have been widely associated with an increased risk of metabolic disorders and overweight. However, a high intake of sources that are rich in monounsaturated fatty acids has been suggested as a dietary agent that is able to positively influence energy metabolism and vascular function. The main objective of this study was to analyze the role of dietary fats on hepatic peptidases activities and metabolic disorders. Three diets: standard (S), HFD supplemented with virgin olive oil (VOO), and HFD supplemented with butter plus cholesterol (Bch), were administered over six months to male Wistar rats. Plasma and liver samples were collected for clinical biochemistry and aminopeptidase activities (AP) analysis. The expression of inducible nitric oxide synthase (iNOS) was also determined by Western blot in liver samples. The diet supplement with VOO did not induce obesity, in contrast to the Bch group. Though the VOO diet increased the time that was needed to return to the basal levels of plasma glucose, the fasting insulin/glucose ratio and HOMA2-%B index (a homeostasis model index of insulin secretion and valuation of β-cell usefulness (% β-cell secretion)) were improved. An increase of hepatic membrane-bound dipeptidyl-peptidase 4 (DPP4) activity was found only in VOO rats, even if no differences in fasting plasma glucagon-like peptide 1 (GLP-1) were obtained. Both HFDs induced changes in hepatic pyroglutamyl-AP in the soluble fraction, but only the Bch diet increased the soluble tyrosyl-AP. Angiotensinase activities that are implicated in the metabolism of angiotensin II (AngII) to AngIV increased in the VOO diet, which was in agreement with the higher activity of insulin-regulated-AP (IRAP) in this group. Otherwise, the diet that was enriched with butter increased soluble gamma-glutamyl transferase (GGT) and Leucyl-AP, iNOS expression in the liver, and plasma NO. In summary, VOO increased the hepatic activity of AP that were related to glucose metabolism (DPP4, angiotensinases, and IRAP). However, the Bch diet increased activities that are implicated in the control of food intake (Tyrosine-AP), the index of hepatic damage (Leucine-AP and GGT), and the expression of hepatic iNOS and plasma NO. Taken together, these results support that the source of fat in the diet affects several peptidases activities in the liver, which could be related to alterations in feeding behavior and glucose metabolism.

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