scholarly journals In vitro studies on different extracts of fenugreek ( Trigonella spruneriana BOISS.): Phytochemical profile, antioxidant activity, and enzyme inhibition potential

2020 ◽  
Vol 44 (11) ◽  
Author(s):  
Volkan Aylanc ◽  
Bulent Eskin ◽  
Gokhan Zengin ◽  
Mehmet Dursun ◽  
Yavuz Selim Cakmak
Keyword(s):  
Antioxidant Activity ◽  
Enzyme Inhibition ◽  
In Vitro Studies ◽  
Phytochemical Profile

scholarly journals Antidiabetic and Antioxidant Activities of Bay, Pandan, Citrus Leaves and Their Combination in Vitro

2019 ◽  
Vol 12 (2) ◽  
pp. 833-841
Author(s):  
Samsu U. Nurdin ◽  
Devi Sabarina ◽  
Subeki Subeki ◽  
Sussi Astuti
Keyword(s):  
Antioxidant Activity ◽  
Enzyme Inhibition ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Diabetic Patients ◽  
Leaf Extracts ◽  
Polyphenol Compounds ◽  
Citrus Leaves

The study aimed to evaluate the effects of bay (B), pandan (P), citrus leaves (C) and their combinations against starch hydrolysis enzymes (α-glucosidase and α-amylase enzymes) and antioxidant activity and to examine the role of polyphenol compounds in enzyme inhibition and antioxidant activity. Three single leaves extracts and five of their combinations were applied to inhibit α-glucosidase hydrolyzing p-nitrophenyl-α-D-glucopyranosyde or α-amylase hydrolyzing starch solution as well as to scavenge free radicals. The leaf extracts and their combination showed inhibition activities against α-glucosidase and α-amylase enzymes with range of inhibition activities were between 17.63% to 26.04% and 20.14% to 35.30% respectively. There is no significantly differ among the extracts in modulation of α-glucosidase activity, but each extract exhibited different effect on α-amylase or antioxidant activities. Mixing P with B and C increases the inhibitory activity of the extract against α-amylase as seen that percent of inhibition of BPC is significantly higher than P, eventhough their total phenolic content was not different. The synergism or antagonism effect was not observed when the extracts were combined as the enzyme inhibition or antioxidant activities are not depend on the proportion of the extract in the mixtures. The role of polyphenol compounds on inhibition of the starch digestion enzymes and on antioxidant activity was not observed. Further study is required to fully elucidate the effect of the leaf or their combinations on diabetic animal models or diabetic patients.

scholarly journals Antioxidant Activity and Phytochemical Characterization of Senecio clivicolus Wedd.

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2497 ◽  
Author(s):  
Immacolata Faraone ◽  
Dilip Rai ◽  
Lucia Chiummiento ◽  
Eloy Fernandez ◽  
Alka Choudhary ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Premature Aging ◽  
In Vitro Assays ◽  
Ms Analysis ◽  
Perennial Shrub ◽  
Health Promoting ◽  
Phytochemical Profile

Antioxidant phytochemicals play a key role in oxidative stress control and in the prevention of related disorders, such as premature aging, degenerative diseases, diabetes, and cancer. The aim of this study was to investigate the potential antioxidant activity and the phytochemical profile of Senecio clivicolus Wedd., a perennial shrub, belonging to the Asteraceae family. Despite the wide interest of this family, this specie has not been investigated yet. S. clivicolus aerial parts were extracted with 96% ethanol. Then, the ethanol extract was fractionated by liquid/liquid extraction using an increasing solvents polarity. Total polyphenol and terpenoid contents were measured. Moreover, the antioxidant activity was evaluated by six different complementary in vitro assays. The Relative Antioxidant Capacity Index (RACI) was used to compare data obtained by different tests. The sample showing the highest RACI was subjected to characterization and quantitation of its phenolic composition using LC-MS/MS analysis. The ethyl acetate fraction, investigated by LC-MS/MS analysis, showed 30 compounds, most of them are chlorogenic acid and flavonoid derivatives. To the best of our knowledge, this is the first report about the evaluation of antioxidant activity and phytochemical profile of S. clivicolus, underlying the importance of this species as a source of health-promoting phytochemicals.

Stability of phenolic content of some herbal infusions and their antioxidant activity following in vitro digestion

2017 ◽  
Vol 42 (4) ◽  
Author(s):  
Engin Celep ◽  
Selin Akyuz ◽  
Yigit İnan ◽  
Erdem Yesilada
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
In Vitro Digestion ◽  
In Vitro Studies ◽  
Vast Number ◽  
The World ◽  
The Stability ◽  
Herbal Infusions

Abstract:Herbal infusions are among the most widely consumed beverages throughout the world. Their popularity arises due to not only their pleasant aroma and taste, but also their high phenolic content and antioxidant activity. A vast number of in vitro studies revealed their high phenolic content, together with strong antioxidant activity. However, the majority of them seem to ignore some important physiological parameters, such as bioavailability or bioaccessibility. The present study was planned to evaluate the stability of phenolic content and antioxidant activities of seven most widely preferred herbal infusions. A stimulated version of gastrointestinal infusion was added to the study for comparative purposes. The results indicated the loss of both phenolic content and antioxidant activity of herbal infusions after being submitted to digestion.

scholarly journals Phytochemical profile and in vitro antioxidant activity of Emelia M (EMB), Mshikazi and Delosma H herbal medicines as demonstrated in THP-1 and Jurkat leukaemia cell lines

2021 ◽  
Vol 21 (4) ◽  
pp. 1924-37
Author(s):  
Joy Nkechinyere Adeniyi ◽  
Manimbulu Nlooto ◽  
Mlungisi Ngcobo ◽  
Roshila Moodley ◽  
Exnevia Gomo
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Herbal Medicines ◽  
Jurkat Cells ◽  
Thiobarbituric Acid Reactive Substance ◽  
Scavenging Activity ◽  
Phytochemical Profile ◽  
In Vitro Antioxidant Activity

Background: Three decoctions, namely Emelia M (EMB), Mshikazi and Delosma H are used by traditional health practitioners in KwaZulu-Natal, South Africa to treat and manage leukaemia and related conditionsObjectives: This study evaluated the in vitro antioxidant activity and phytochemical profile of the aqueous extracts of Emelia M (EMB), Mshikazi and Delosma H decoctions.Methods: Antioxidant activity of the extracts was evaluated using1-diphenyl-2-picrylhydrazyl (DPPH), glutathione (GSH), phosphomolybdate and thiobarbituric acid reactive substance (TBARS) assays. Phytochemical screening was used to determinethe presence of compounds.Results: The DPPH radical scavenging activity was similar to ascorbic acid for EMB and Delosma H, but not for Mshikazi. At 24 h, EMB increased GSH in both THP-1 and Jurkat cells similar to Delosma H while Mshikazi demonstrated the lowest activity. At 48 h, EMB and Delosma H revealed increased GSH in THP-1 cells with no significant decrease in GSH levels in Jurkat cells. However, EMB showed the lowest lipid peroxidation activity compared to Delosma H and Mshikazi after 24 h treatment of both cells. Phenols, flavonoids, terpenoids, saponins were present in all extracts.Conclusion: Extracts of the three decoctions possess both antioxidant and prooxidant properties through high scavenging activity and increased in lipid peroxidation. Keywords: Antioxidants; herbal medicines; Emelia M; Mshikazi; Delosma H.

scholarly journals Oleuropein-Laded Ufasomes Improve the Nutraceutical Efficacy

Nanomaterials ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 105
Author(s):  
Maria Chiara Cristiano ◽  
Francesca Froiio ◽  
Antonia Mancuso ◽  
Donato Cosco ◽  
Luciana Dini ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cell Membranes ◽  
Unsaturated Fatty Acids ◽  
Dynamic Structure ◽  
Biological Properties ◽  
In Vitro Studies ◽  
Antioxidant Compounds ◽  
Transmission Electron ◽  
Physico Chemical

Ufasomes are unsaturated fatty acid liposomes made up of oleic and linoleic acids, natural components required in various biological processes. This kind of nanocarrier is characterized by a simple and dynamic structure and is able to improve the bioavailability of unsaturated fatty acids. The aim of this investigation was to evaluate ufasomes as natural compound delivery systems to deliver oleuropein and improve its antioxidant activity. Oleuropein is a phenolic compound mainly present in olives and olive oil, with several biological properties, such as the antioxidant activity. However, to improve their biological activity, antioxidant compounds should be able to cross cell membranes and uniformly incorporate in cells. Because of the great similarity between their constituents and cell membranes, ufasomes could be advantageous carriers for oleuropein delivery. The physico-chemical characteristics of ufasomes were investigated. A regular shape was shown by transmission electron microscopy studies, while the mean sizes were dependent on the ufasomes composition. In vitro studies highlighted that empty ufasomes did not lead to cell mortality at the tested concentrations and a good carrier internalization in CaCo-2 cells, further studies in vitro studies demonstrated that oleuropein-loaded ufasomes were able to enhance the antioxidant activity of the free active substance making this carrier a suitable one for nutraceutical application.

In Vivo and In Vitro Studies on the Antioxidant Activity of Aloin Compared to Doxorubicin in Rats

2012 ◽  
Vol 73 (3) ◽  
pp. 154-165 ◽  
Author(s):  
Amr Y. Esmat ◽  
Mahmoud M. Said ◽  
Germine M. Hamdy ◽  
Amel A. Soliman ◽  
Sally A. Khalil
Keyword(s):  
Antioxidant Activity ◽  
In Vitro Studies

scholarly journals Antioxidant and Hypolipidemic Effect ofPlumeria RubraL. in Alloxan Induced Hyperglycemic Rats

2010 ◽  
Vol 7 (1) ◽  
pp. 1-5 ◽  
Author(s):  
A. John Merina ◽  
D. Sivanesan ◽  
V. Hazeena Begum ◽  
N. Sulochana
Keyword(s):  
Antioxidant Activity ◽  
Serum Cholesterol ◽  
No Reduction ◽  
In Vitro Studies ◽  
Hypolipidemic Activity ◽  
Hypolipidemic Effect ◽  
Serum Triglycerides ◽  
Plumeria Rubra

Antioxidant and hypolipidemic activity of the flovone glycoside isolated fromPlumeria rubraL. was carried in alloxan induced hyperglycemic rats. The flavonoid treatment produced a significant reduction in the level of serum triglycerides, while there was no reduction in the serum cholesterol and glucose. Antioxidant activity of the drug was also confirmed throughin vitro studies.

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