STUDY OF GROWTH AND N, P CONTENT OF MICROALGAE Chlorella vulgaris CULTIVATED IN DIFFERENT CULTURE MEDIA AND LIGHT INTENSITY

2016 ◽  
Vol 78 (4-2) ◽  
Author(s):  
Dedi Edwin Satriaji ◽  
Muhammad Zainuri ◽  
Ita Widowati
Keyword(s):  
Growth Rate ◽  
Light Intensity ◽  
Specific Growth Rate ◽  
Chlorella Vulgaris ◽  
Specific Growth ◽  
Culture Media ◽  
Algal Growth ◽  
Nitrogen And Phosphorus ◽  
Cell Content ◽  
Media Type

Different light intensity and different media types were used to test their effects on algal growth and biochemical composition of cell content to optimize the growth of algal biomass production. Analysis of the results includes growth calculation of Chlorella vulgaris obtained from 6 d observations for 12 h a day (06:00 am to 06:00 pm) with 3 h breaks in between observation. Laboratory analysis was done at the end of day 5th to determine the content of N, and P. Density of C. vulgaris were influenced by both light intensity and media. Differences media type and light intensity as separated parameters and their interactions significantly (p < 0.05) affected on the growth and nitrogen and phosphor content of C. vulgaris. The highest density of  2 310 × 104 cells · mL–1 was on day 4 with walne media resulting in specific growth rate (µ) of 0.43 per day with a  light intensity of 5 000 lux, and the lowest density of 725 × 104 cells · mL–1 was in day 4 with the NPK media and specific growth rate (µ) 0.25 per day with a light intensity of 4 000 lux. The highest nitrogen and phosphorus content (16.12 mg and 28.19 mg) was obtained from walne medium with a light intensity of 5000 lux, and the lowest (3.43 mg and 2.17 mg) was obtained from NPK with a light intensity of 4 000 lux.

Effect of a Freshwater Bacterium on Four Microcystis under Eutrophic Conditions

2013 ◽  
Vol 295-298 ◽  
pp. 1636-1642
Author(s):  
Wan Sheng Zou ◽  
Yu Shuang Luo ◽  
Liang Guo Liu ◽  
Wen Bin Wang ◽  
Pin Hong Yang
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Bacterial Strain ◽  
Specific Growth ◽  
Culture Media ◽  
Bacterial Flora ◽  
Cyanobacterial Blooms ◽  
Dynamic Changes ◽  
Microcystis Blooms ◽  
Cell Densities

The effect of a freshwater bacterium Exiguobacterium sp.013 was examined on the cultures of four freshwater Microcystis which can cause cyanobacterial blooms. Four freshwater Microcystis cells and the bacterium were inoculated simultaneously, in modified BG-11 culture media, and cultured for 24 days continuously. The specific growth rate and cell densities were employed and determined in treated cultures and in controls.Additionally, dynamic changes of bacterium were investigated during the whole cultures. The results showed that the specific growth rate and maximal cell density of M. aeruginosa, M. flos-aquae and M. wesenbergii in treated cultures were significantly enhanced than that of control cultures, and daily cell densities of four Microcystis in the treated cultures were also significantly higher compared with that of control cultures. The added bacterial strain always kept dominance among the bacterial flora during the culture period. These findings implied that bacteria Exiguobacterium sp. might play a positive promoting role in forming Microcystis blooms under the eutrophication condition.

scholarly journals Effect of Adding BIOM-S Probiotic on Survival and Growth Rate of Nile Tilapia (Oreochromis niloticus)

2018 ◽  
pp. 1-6
Author(s):  
Yuli Andriani ◽  
Taufik Ikhsan Kamil ◽  
Irfan Zidni ◽  
Iskandar . ◽  
Titin Herawati
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Specific Growth Rate ◽  
Nile Tilapia ◽  
Specific Growth ◽  
Culture Media ◽  
Control Treatment ◽  
Randomized Design ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Survival And Growth

This research was conducted in May-June 2017 in Hatchery Indoor Maksudi, Astanaanyar, Bandung. This research aims to find out the optimal BIOM-S probiotic on culture media of Nile tilapia, and the influence to survival rate and growth rate of Nile tilapia. This research was conducted by an experiment using Completely Randomized Design (CRD). This research consisted of five treatments and three replications, which were treatment A (control), treatment B (giving probiotic with 0,6 ml/L concentration), treatment C (giving probiotic with 0,8 ml/L concentration), treatment D (giving probiotic with ,0 ml/L concentration), and treatment E (giving probiotic with 1,2 ml/L concentration). The parameters in this research were the survival rate and specific growth rate. Survival rate and specific growth rate used data analysis with F test to find out the influence of each treatment. The concentration of optimal probiotic based on the results was 0.8 ml/L, it produced the highest survival rate for 81.67% and the specific growth rate for 0.039%.

scholarly journals Effect of Physical Factors on the Growth of Chlorella Vulgaris on Enriched Media Using the Methods of Orthogonal Analysis and Response Surface Methodology

Water ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 34
Author(s):  
Lile He ◽  
Yongcan Chen ◽  
Xuefei Wu ◽  
Shu Chen ◽  
Jing Liu ◽  
...  
Keyword(s):  
Growth Rate ◽  
Particle Size ◽  
Response Surface Methodology ◽  
Specific Growth Rate ◽  
Chlorella Vulgaris ◽  
Specific Growth ◽  
Liquid Ratio ◽  
Physical Conditions ◽  
Orthogonal Analysis ◽  
Solid Liquid

In addition to chemical factors, physical conditions also play a key role in the growth of microalgae. In this study, solid sediment in rivers was simulated by pure quartz sand with different particle sizes and the physical effects of disturbance rate, solid–liquid ratio and particle size on the growth of Chlorella vulgaris (C. vulgaris) were investigated through orthogonal analysis and response surface methodology (RSM) during co-cultivation of C. vulgaris and sediment. The result of ANOVA in orthogonal analysis showed that the effect ability of a single factor on biomass can be ranked as disturbance rate > particle size > solid–liquid ratio, 100 r/min disturbance rate and 30–40 M particle size are the most significant at the 0.05 level. Furthermore, the specific growth rate can reach 0.25/d and 0.27/d, respectively. With the growth of C. vulgaris, the pH of the solution reached a maximum of 10.7 in a week. The results from the RSM showed that strong interactions are reflected in the combinations of disturbance rate and solid–liquid ratio, and disturbance rate and particle size. Ramp desirability of the biomass indicates that the optimum levels of the three variables are 105 r/min disturbance rate, 0.117 g/mL solid–liquid ratio and 30–40 M particle size. In this case, the biomass can grow seven times in a week with 0.27/d specific growth rate and a pH value of 7–10.4. This study shows that the growth of C. vulgaris can be regulated by changing physical conditions simultaneously, and the optimization of physical conditions can be applied to biomass production, algae prediction and acid water treatment in rivers, lakes and reservoirs.

scholarly journals Effect of soil extracts concentration on specific growth rate and lipid content of chlorella vulgaris in bolds basal medium

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Ani Idris ◽  
M. Atta ◽  
A. Bukhari
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Chlorella Vulgaris ◽  
Mass Culture ◽  
Specific Growth ◽  
Basal Medium ◽  
Dry Weight ◽  
Soil Extract ◽  
Soil Extracts ◽  
Key Factor

Effective nutrient medium is the key factor that significantly influences the specific growth rate and the final concentration of microalgae. The main objective of this study was to optimize the microalgal growth in mass culture system under the effect of soil extract in modified Bolds Basal medium. Chlorella vulgaris was grown aseptically for 10 days at five different concentrations of soil extract (10, 20, 30, 40, 50ml/L) at 25°C cultivation temperature. After 10 days of cultivation, Chlorella vulgaris showed maximum cell concentration of 5x107/ml at 30ml/L of soil extract concentration which corresponds to the maximum specific growth rate 1.56μd-1 with the doubling rate of 2.25d-1   andlipid yield of 22.74 % of dry weight.________________________________________GRAPHICAL ABSTRACT

scholarly journals Potensi Rumput Laut Eucheuma sp. Terhadap Kepadatan Fitoplankton Chlorella sp.

2016 ◽  
Vol 18 (3) ◽  
pp. 166
Author(s):  
Ria Azizah ◽  
Indrihastuti Sulistianingtiyas ◽  
Delianis Pringgenies ◽  
Siti Rudiyanti
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Research Method ◽  
Specific Growth ◽  
Culture Media ◽  
Chlorella Sp ◽  
Materials Used ◽  
Marine Exploration

Chlorella sp. merupakan sumber makanan bagi ikan dan udang. Untuk menumbuhkan, Chlorella sp. maka diperlukan media kultur dengan nutrien yang baik. Eucheuma sp. merupakan rumput laut yang mengandung mineral - mineral yang bermanfaat bagi pertumbuhan Chlorella sp. Pemberian ekstrak Eucheuma sp. diharapkan dapat mendukung pertumbuhan Chlorella sp. Tujuan dilakukan penelitian untuk mengetahui pengaruh ekstrak Eucheuma sp. terhadap kepadatan Chlorella sp. dan konsentrasi terbaik dari ekstrak Eucheuma sp. yang menghasilkan kepadatan Chlorella sp. yang tertinggi. Penelitian ini dilaksanakan pada bulan Januari - Maret 2005 di Laboratorium Bioteknologi dan Eksplorasi Laut, Teluk Awur, UNDIP, Jepara dan Laboratorium Jurusan Perikanan, UNDIP, Semarang. Materi yang digunakan yaitu ekstrak Eucheuma sp., Chlorella sp. dan media kultur. Metode yang digunakan adalah eksperimental laboratories. Konsentrasi ekstrak Eucheuma sp. yang digunakan adalah : A (10 mg/L); B (100 mg/L); C (200 mg/L); D (300 mg/L); E (400 mg/L); F (500 mg/L); G ( tanpa pemberian ekstrak Eucheuma sp.).Hasil penelitian menunjukkan perlakuan D (300 mg/L) menghasilkan kepadatan Chlorella sp. tertinggi pada puncak populasi, yaitu 6,293 log sel/mL dan pada akhir populasi, yaitu 6,012 log sel/mL dengan konstanta pertumbuhan spesifik sebesar 0,161.Kata kunci: Kepadatan, Chlorella, EucheumaChlorella sp. is the source of food to prawn and fish. In order to grow Chlorella sp. so need a culture media with a good nutrient. Eucheuma sp. is a seaweed it cantains benefit minerals for the growth of Chlorella sp. The giving of Eucheuma sp. it is to be hoped to support the the growth of Chlorella sp. This research is aimed to know the effect of Eucheuma sp. extract to density of Chlorella sp. and the best concentration of Eucheuma sp. extract produce the highest density of Chlorella sp. This research was conducted in January – March 2005 at the Laboratory of Biotechnology and Marine Exploration, Teluk Awur, UNDIP, Jepara and Laboratory of Fisheries Department UNDIP, Semarang. Materials used in this research consisted of Eucheuma sp. extract, Chlorella sp. and culture media. This research method used experimental laboratories. The concentration that used in this research : A (10 mg/L); B (100 mg/L); C (200 mg/L); D (300 mg/L); E (400 mg/L); F (500 mg/L); G ( without giving extract of Eucheuma sp.). The result of this research showed that the D treatment produce the highest Chlorella sp. density at peak of population, that is 6,293 log sel/mL and the end of population that is 6,012 log sel/mL with specific growth rate 0,161.Keyword : Density, Chlorella., Eucheuma

scholarly journals Dynamics Modeling of Nitrogen Compounds in Microalgae Cells. 2. Chemostat

2019 ◽  
Vol 14 (2) ◽  
pp. 450-463 ◽  
Author(s):  
A.S. Lelekov ◽  
R.P. Trenkenshu
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Specific Growth ◽  
Nitrogen Compounds ◽  
Specific Rate ◽  
Dynamics Modeling ◽  
Cell Content ◽  
Classical Models ◽  
Species Specific ◽  
Maximum Specific Rate

The work focuses on dynamics modeling of nitrogen compounds in microalgae cells under chemostat conditions. The analysis of classical models (Michaelis-Menten, Monod, Droop), which describe the kinetics of substrate-dependent growth of microalgae, was carried out. Classical models are shown to be applicable provided that the physicochemical parameters of the medium such as temperature, cell irradiation, etc are constant. As an alternative approach, the possibility of using linear splines in kinetics modeling of nitrate absorption by microalgae is shown. For the conditions of the chemostat, particular solutions for the generalized model of the dynamics of nitrogen compounds in the cells of microalgae considered in the first part of the work were obtained, and boundary conditions for the culture growth at unlimited nitrogen were determined. For limited growth, the equation for both the dependence of the specific growth rate on the intracellular nitrogen content, which coincides in form with the Droop model and the dependence of the specific growth rate on the extracellular concentration of nitrogen, which coincides in form with the Monod model were obtained. The species-specific coefficients of the equations such as the maximum specific rate of nitrogen absorption, the maximum specific rate of synthesis of structural components, the maximum content of reserve forms of nitrogen, the minimum share of structural forms of nitrogen in the total cell content were determined.

scholarly journals Growth and secondary metabolites content of chloroform extract of Chlorella sp. and Chlorella sorokiniana cultured on chicken broiler waste media

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Devy Susanty ◽  
Ade Ayu Oksari
Keyword(s):  
Growth Rate ◽  
Secondary Metabolites ◽  
Specific Growth Rate ◽  
Cell Density ◽  
Specific Growth ◽  
Culture Media ◽  
Chloroform Extract ◽  
Chlorella Sorokiniana ◽  
Phytochemical Content ◽  
Chlorella Sp

Abstract. Susanty D, Oksari AA. 2020. Growth and secondary metabolites content of chloroform extract of Chlorella sp. and Chlorella sorokiniana cultured on chicken broiler waste media. Nusantara Bioscience 12: 28-32. Intensive chicken broiler farms create waste in the environment. Chicken Broiler Waste (CBW) was used as culture media for Chlorella sp. InaCC M39 and Chlorella sorokiniana InaCC M38 at various concentrations (2, 4, 6, 8, and 10%), and compared to AF6 media. The growth of Chlorella was observed every 48 hours for 10 days by counting the number of cells using a hemocytometer. The chloroform extract of Chlorella sp. and C. sorokiniana were analyzed for their phytochemical content to determine the presence of alkaloids, flavonoids, saponin, steroids, and tannin. The results showed that 2% of CBW media was the best medium for the growth of Chlorella with nitrogen (N), phosphorus (P), and potassium (K) content was 0.01% N; 0.01% P; 0.06% K respectively. The highest specific growth rate of Chlorella sp. on 2% CBW medium was on the 8th day of culture (0.8133) with cell density of 1.6 x 106 cells/mL, while the highest specific growth rate of Chlorella sorokiniana was on the 6th day (0.8907) with cell density of 2.99 x 107 cells/mL. The chloroform extract of the biomass of Chlorella sorokiniana contained steroid and saponin

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