Potensi Rumput Laut  Eucheuma sp. Terhadap  Kepadatan Fitoplankton Chlorella sp.

Chlorella sp. merupakan sumber makanan bagi ikan dan udang. Untuk menumbuhkan, Chlorella sp. maka diperlukan media kultur dengan nutrien yang baik. Eucheuma sp. merupakan rumput laut yang mengandung mineral - mineral yang bermanfaat bagi pertumbuhan Chlorella sp. Pemberian ekstrak Eucheuma sp. diharapkan dapat mendukung pertumbuhan Chlorella sp. Tujuan dilakukan penelitian untuk mengetahui pengaruh ekstrak Eucheuma sp. terhadap kepadatan Chlorella sp. dan konsentrasi terbaik dari ekstrak Eucheuma sp. yang menghasilkan kepadatan Chlorella sp. yang tertinggi. Penelitian ini dilaksanakan pada bulan Januari - Maret 2005 di Laboratorium Bioteknologi dan Eksplorasi Laut, Teluk Awur, UNDIP, Jepara dan Laboratorium Jurusan Perikanan, UNDIP, Semarang. Materi yang digunakan yaitu ekstrak Eucheuma sp., Chlorella sp. dan media kultur. Metode yang digunakan adalah eksperimental laboratories. Konsentrasi ekstrak Eucheuma sp. yang digunakan adalah : A (10 mg/L); B (100 mg/L); C (200 mg/L); D (300 mg/L); E (400 mg/L); F (500 mg/L); G ( tanpa pemberian ekstrak Eucheuma sp.).Hasil penelitian menunjukkan perlakuan D (300 mg/L) menghasilkan kepadatan Chlorella sp. tertinggi pada puncak populasi, yaitu 6,293 log sel/mL dan pada akhir populasi, yaitu 6,012 log sel/mL dengan konstanta pertumbuhan spesifik sebesar 0,161.Kata kunci: Kepadatan, Chlorella, EucheumaChlorella sp. is the source of food to prawn and fish. In order to grow Chlorella sp. so need a culture media with a good nutrient. Eucheuma sp. is a seaweed it cantains benefit minerals for the growth of Chlorella sp. The giving of Eucheuma sp. it is to be hoped to support the the growth of Chlorella sp. This research is aimed to know the effect of Eucheuma sp. extract to density of Chlorella sp. and the best concentration of Eucheuma sp. extract produce the highest density of Chlorella sp. This research was conducted in January – March 2005 at the Laboratory of Biotechnology and Marine Exploration, Teluk Awur, UNDIP, Jepara and Laboratory of Fisheries Department UNDIP, Semarang. Materials used in this research consisted of Eucheuma sp. extract, Chlorella sp. and culture media. This research method used experimental laboratories. The concentration that used in this research : A (10 mg/L); B (100 mg/L); C (200 mg/L); D (300 mg/L); E (400 mg/L); F (500 mg/L); G ( without giving extract of Eucheuma sp.). The result of this research showed that the D treatment produce the highest Chlorella sp. density at peak of population, that is 6,293 log sel/mL and the end of population that is 6,012 log sel/mL with specific growth rate 0,161.Keyword : Density, Chlorella., Eucheuma

Download Full-text

Growth and secondary metabolites content of chloroform extract of Chlorella sp. and Chlorella sorokiniana cultured on chicken broiler waste media

Nusantara Bioscience ◽

10.13057/nusbiosci/n120105 ◽

2020 ◽

Vol 12 (1) ◽

Author(s):

Devy Susanty ◽

Ade Ayu Oksari

Keyword(s):

Growth Rate ◽

Secondary Metabolites ◽

Specific Growth Rate ◽

Cell Density ◽

Specific Growth ◽

Culture Media ◽

Chloroform Extract ◽

Chlorella Sorokiniana ◽

Phytochemical Content ◽

Chlorella Sp

Abstract. Susanty D, Oksari AA. 2020. Growth and secondary metabolites content of chloroform extract of Chlorella sp. and Chlorella sorokiniana cultured on chicken broiler waste media. Nusantara Bioscience 12: 28-32. Intensive chicken broiler farms create waste in the environment. Chicken Broiler Waste (CBW) was used as culture media for Chlorella sp. InaCC M39 and Chlorella sorokiniana InaCC M38 at various concentrations (2, 4, 6, 8, and 10%), and compared to AF6 media. The growth of Chlorella was observed every 48 hours for 10 days by counting the number of cells using a hemocytometer. The chloroform extract of Chlorella sp. and C. sorokiniana were analyzed for their phytochemical content to determine the presence of alkaloids, flavonoids, saponin, steroids, and tannin. The results showed that 2% of CBW media was the best medium for the growth of Chlorella with nitrogen (N), phosphorus (P), and potassium (K) content was 0.01% N; 0.01% P; 0.06% K respectively. The highest specific growth rate of Chlorella sp. on 2% CBW medium was on the 8th day of culture (0.8133) with cell density of 1.6 x 106 cells/mL, while the highest specific growth rate of Chlorella sorokiniana was on the 6th day (0.8907) with cell density of 2.99 x 107 cells/mL. The chloroform extract of the biomass of Chlorella sorokiniana contained steroid and saponin

Download Full-text

Effect of a Freshwater Bacterium on Four Microcystis under Eutrophic Conditions

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.295-298.1636 ◽

2013 ◽

Vol 295-298 ◽

pp. 1636-1642

Author(s):

Wan Sheng Zou ◽

Yu Shuang Luo ◽

Liang Guo Liu ◽

Wen Bin Wang ◽

Pin Hong Yang

Keyword(s):

Growth Rate ◽

Specific Growth Rate ◽

Bacterial Strain ◽

Specific Growth ◽

Culture Media ◽

Bacterial Flora ◽

Cyanobacterial Blooms ◽

Dynamic Changes ◽

Microcystis Blooms ◽

Cell Densities

The effect of a freshwater bacterium Exiguobacterium sp.013 was examined on the cultures of four freshwater Microcystis which can cause cyanobacterial blooms. Four freshwater Microcystis cells and the bacterium were inoculated simultaneously, in modified BG-11 culture media, and cultured for 24 days continuously. The specific growth rate and cell densities were employed and determined in treated cultures and in controls.Additionally, dynamic changes of bacterium were investigated during the whole cultures. The results showed that the specific growth rate and maximal cell density of M. aeruginosa, M. flos-aquae and M. wesenbergii in treated cultures were significantly enhanced than that of control cultures, and daily cell densities of four Microcystis in the treated cultures were also significantly higher compared with that of control cultures. The added bacterial strain always kept dominance among the bacterial flora during the culture period. These findings implied that bacteria Exiguobacterium sp. might play a positive promoting role in forming Microcystis blooms under the eutrophication condition.

Download Full-text

Effect of Adding BIOM-S Probiotic on Survival and Growth Rate of Nile Tilapia (Oreochromis niloticus)

Asian Journal of Fisheries and Aquatic Research ◽

10.9734/ajfar/2018/v2i226120 ◽

2018 ◽

pp. 1-6

Author(s):

Yuli Andriani ◽

Taufik Ikhsan Kamil ◽

Irfan Zidni ◽

Iskandar . ◽

Titin Herawati

Keyword(s):

Growth Rate ◽

Survival Rate ◽

Specific Growth Rate ◽

Nile Tilapia ◽

Specific Growth ◽

Culture Media ◽

Control Treatment ◽

Randomized Design ◽

Nile Tilapia Oreochromis Niloticus ◽

Survival And Growth

This research was conducted in May-June 2017 in Hatchery Indoor Maksudi, Astanaanyar, Bandung. This research aims to find out the optimal BIOM-S probiotic on culture media of Nile tilapia, and the influence to survival rate and growth rate of Nile tilapia. This research was conducted by an experiment using Completely Randomized Design (CRD). This research consisted of five treatments and three replications, which were treatment A (control), treatment B (giving probiotic with 0,6 ml/L concentration), treatment C (giving probiotic with 0,8 ml/L concentration), treatment D (giving probiotic with ,0 ml/L concentration), and treatment E (giving probiotic with 1,2 ml/L concentration). The parameters in this research were the survival rate and specific growth rate. Survival rate and specific growth rate used data analysis with F test to find out the influence of each treatment. The concentration of optimal probiotic based on the results was 0.8 ml/L, it produced the highest survival rate for 81.67% and the specific growth rate for 0.039%.

Download Full-text

Cultivation of Chlorella sp. as Biofuel Sources in Palm Oil Mill Effluent (POME)

International Journal of Renewable Energy Development ◽

10.14710/ijred.1.2.45-49 ◽

2012 ◽

Vol 1 (2) ◽

pp. 45-49 ◽

Cited By ~ 14

Author(s):

H Hadiyanto ◽

Muhamad Maulana Azimatun Nur ◽

Ganang Dwi Hartanto

Keyword(s):

Growth Rate ◽

Renewable Energy ◽

Specific Growth Rate ◽

Nitrogen Source ◽

Palm Oil ◽

Specific Growth ◽

Urea Concentration ◽

Palm Oil Mill Effluent ◽

Chlorella Sp ◽

Palm Oil Mill

Renewable energy is essential and vital aspect for development in Indonesia especially less oil reserve for coming 15 years. Biodiesel has received much attention as renewable energy in recent years. One of potential biodiesel is produced from microalgae. Due to high content of nutrients in Palm Oil Mill Effluent (POME), this waste is a potential for nutrient growth for microalgae. Chlorella is one of high potential for biodiesel since it has high lipid content (20-30%). The objective of the research is to determine growth rate and biomass productivity in Chlorella Sp cultured in POME. Chlorella Sp was cultured in 20%, 50%, 70% POME using urea concentration 0.1gr/L (low nitrogen source) and 1gr/l (high nitrogen source) at flask disk, pH 6.8-7.2; aerated using aquarium pump and fluorescence lamp 3000-6000 lux as light. Medium was measured using spectrophotometer Optima Sp-300 OD at 680 wave length in 15 days to calculate specific growth rate. At end of cultivation, Chlorella sp was filtered and measured as dry weight. Result indicated that Chlorella sp at 50% POME 1gr/L urea showed higher specific growth rate (0.066/day). Factor affecting growth rate of microalgae is CNP ratio, POME concentration, and urea concentration.

Download Full-text

Optimization of Culture Conditions on Growth of Chlorella sp. Newly Isolated From Bagansiapiapi Waters Indonesia

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/934/1/012097 ◽

2021 ◽

Vol 934 (1) ◽

pp. 012097

Author(s):

D Iriani ◽

B Hasan ◽

HS Putra ◽

TM Ghazali

Keyword(s):

Growth Rate ◽

Specific Growth Rate ◽

Cell Density ◽

Specific Growth ◽

Pharmaceutical Products ◽

Culture Conditions ◽

Marine Waters ◽

Chlorella Sp ◽

Need To Evaluate ◽

Further Development

Abstract Chlorella sp. is a type of micro-sized green algae obtained in fresh or marine waters. However, many factors need to evaluate the growth of Chlorella sp. mainly the culture conditions. This study aimed to evaluate the culture conditions in optimizing the growth of Chlorella sp. newly isolated from Bagansiapiapi marine waters. The experiment was carried out at temperature ±25°C, light intensity 2300 Lux using TL-D lamp (36 W). There were six treatments as culture conditions for the cultivation of Chlorella sp.:1). Bean Sprouts (Vigna radiata) Extract Media (BSEM) with light continuously, 2. BSEM with a Photoperiod light:dark (10:14h), 3. Tofu waste media with light continuously, 4. Tofu waste media with a photoperiod light:dark (10: 14h), 5. Fish pellet solutions media (25%), 6. Fish pellet solutions media (50%). The parameter observed was the growth, cell density and the specific growth rate of Chlorella sp. every 2 days for 14 days of cultivation. Based on the results shown that the marine Chlorella sp. from Bagansiapiapi waters could be adapted to all treatments. Nevertheless, the use of BSEM light continuously obtained the highest cell density (27.75 x 105 cell/ml) on 12 days and continues to increase until 14 days, while the other treatments had decreased. Furthermore, the Chlorella sp. obtained the specific growth rate was 0.42. Therefore, the marine Chlorella sp. from Bagansiapiapi could be cultivated in BSEM light continuously for further development as a functional food or bioactive source in pharmaceutical products.

Download Full-text

Metode Lepas Dasar dengan Net Bag pada Pertumbuhan Kappaphycus alvarezii (Doty)

Journal of Marine Research ◽

10.14710/jmr.v9i1.25783 ◽

2020 ◽

Vol 9 (1) ◽

pp. 49-54

Author(s):

Ichsan Suryo Wibowo ◽

Gunawan Widi Santosa ◽

Ali Djunaedi

Keyword(s):

Growth Rate ◽

Specific Growth Rate ◽

Research Method ◽

Specific Growth ◽

Kappaphycus Alvarezii ◽

Quality Parameters ◽

Test Results ◽

Seaweed Farming ◽

Randomized Design ◽

Completely Randomized Design

ABSTRAK: Parameter keberhasilan dari kegiatan budidaya rumput laut diukur berdasarkan dari hasil produksi thallus yang dibudidayakan. Metode budidaya yang digunakan dalam kegiatan budidaya rumput laut merupakan salah satu faktor penting yang dijadikan sebagai acuan keberhasilan. Penelitian ini bertujuan untuk mengkaji pertumbuhan dari rumput laut K. alvarezii yang dibudidayakan dengan metode lepas dasar menggunakan net bag yang dimodifikasi. Materi yang digunakan dalam penelitian ini adalah bibit rumput laut K. alvarezii (F2) yang diperoleh dari pembudidaya di Perairan Pulau Kemujan, Kepulauan Karimunjawa. Metode penelitian yang digunakan adalah eksperimen dengan rancangan percobaan berpola Rancangan Acak Lengkap. Perlakuan yang diujikan adalah rumput laut K. alvarezii yang dibudidayakan dengan perlakuan A (satu lapis jaring), perlakuan B (dua lapis jaring), dan perlakuan C (tiga lapis jaring). Penanaman bibit dilakukan selama 42 hari sejak penanaman. Parameter pengamatan meliputi laju pertumbuhan spesifik dan parameter kualitas perairan. Hasil penelitian menunjukkan terdapat pengaruh sistem budidaya lepas dasar menggunakan net bag (P < 0,05) terhadap laju pertumbuhan spesifik K. alvarezii. Metode net bag dengan satu lapis jaring memiliki pertumbuhan terbaik pada 4,95 ± 0,70 %g/hari. ABSTRACT: The success parameters of seaweed farming activities are measured based on the results of cultivated thallus. The cultivation method used in seaweed farming activities is one of the important factors used as a reference for success. This study aimed to assess the growth of K. alvarezii that was cultivated by employing off bottom method in a modified net bag system. The material used in this study was K. alvarezii (F2) seaweed seeds obtained from cultivators in Kemujan Island, Karimunjawa Islands. The research method used was an experiment with a completely randomized design patterned. The treatments tested were K. alvarezii which was cultivated by treatment A (single net), treatment B (double net), and treatment C (triple net). Planting of seeds was carried out for 42 days after planting. Observation parameters include specific growth rate and water quality parameters. ANOVA test results showed that there was a significant influence (P <0.05) on the absolute weight and specific growth rate of K. alvarezii. Results showed that single net bag method given the best growth rate at 4.95 ± 0.70 %g/day.

Download Full-text

STUDY OF GROWTH AND N, P CONTENT OF MICROALGAE Chlorella vulgaris CULTIVATED IN DIFFERENT CULTURE MEDIA AND LIGHT INTENSITY

Jurnal Teknologi ◽

10.11113/jt.v78.8148 ◽

2016 ◽

Vol 78 (4-2) ◽

Author(s):

Dedi Edwin Satriaji ◽

Muhammad Zainuri ◽

Ita Widowati

Keyword(s):

Growth Rate ◽

Light Intensity ◽

Specific Growth Rate ◽

Chlorella Vulgaris ◽

Specific Growth ◽

Culture Media ◽

Algal Growth ◽

Nitrogen And Phosphorus ◽

Cell Content ◽

Media Type

Different light intensity and different media types were used to test their effects on algal growth and biochemical composition of cell content to optimize the growth of algal biomass production. Analysis of the results includes growth calculation of Chlorella vulgaris obtained from 6 d observations for 12 h a day (06:00 am to 06:00 pm) with 3 h breaks in between observation. Laboratory analysis was done at the end of day 5th to determine the content of N, and P. Density of C. vulgaris were influenced by both light intensity and media. Differences media type and light intensity as separated parameters and their interactions significantly (p < 0.05) affected on the growth and nitrogen and phosphor content of C. vulgaris. The highest density of 2 310 × 104 cells · mL–1 was on day 4 with walne media resulting in specific growth rate (µ) of 0.43 per day with a light intensity of 5 000 lux, and the lowest density of 725 × 104 cells · mL–1 was in day 4 with the NPK media and specific growth rate (µ) 0.25 per day with a light intensity of 4 000 lux. The highest nitrogen and phosphorus content (16.12 mg and 28.19 mg) was obtained from walne medium with a light intensity of 5000 lux, and the lowest (3.43 mg and 2.17 mg) was obtained from NPK with a light intensity of 4 000 lux.

Download Full-text