scholarly journals Growth and secondary metabolites content of chloroform extract of Chlorella sp. and Chlorella sorokiniana cultured on chicken broiler waste media

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Devy Susanty ◽  
Ade Ayu Oksari
Keyword(s):  
Growth Rate ◽  
Secondary Metabolites ◽  
Specific Growth Rate ◽  
Cell Density ◽  
Specific Growth ◽  
Culture Media ◽  
Chloroform Extract ◽  
Chlorella Sorokiniana ◽  
Phytochemical Content ◽  
Chlorella Sp

Abstract. Susanty D, Oksari AA. 2020. Growth and secondary metabolites content of chloroform extract of Chlorella sp. and Chlorella sorokiniana cultured on chicken broiler waste media. Nusantara Bioscience 12: 28-32. Intensive chicken broiler farms create waste in the environment. Chicken Broiler Waste (CBW) was used as culture media for Chlorella sp. InaCC M39 and Chlorella sorokiniana InaCC M38 at various concentrations (2, 4, 6, 8, and 10%), and compared to AF6 media. The growth of Chlorella was observed every 48 hours for 10 days by counting the number of cells using a hemocytometer. The chloroform extract of Chlorella sp. and C. sorokiniana were analyzed for their phytochemical content to determine the presence of alkaloids, flavonoids, saponin, steroids, and tannin. The results showed that 2% of CBW media was the best medium for the growth of Chlorella with nitrogen (N), phosphorus (P), and potassium (K) content was 0.01% N; 0.01% P; 0.06% K respectively. The highest specific growth rate of Chlorella sp. on 2% CBW medium was on the 8th day of culture (0.8133) with cell density of 1.6 x 106 cells/mL, while the highest specific growth rate of Chlorella sorokiniana was on the 6th day (0.8907) with cell density of 2.99 x 107 cells/mL. The chloroform extract of the biomass of Chlorella sorokiniana contained steroid and saponin

scholarly journals Potensi Rumput Laut Eucheuma sp. Terhadap Kepadatan Fitoplankton Chlorella sp.

2016 ◽  
Vol 18 (3) ◽  
pp. 166
Author(s):  
Ria Azizah ◽  
Indrihastuti Sulistianingtiyas ◽  
Delianis Pringgenies ◽  
Siti Rudiyanti
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Research Method ◽  
Specific Growth ◽  
Culture Media ◽  
Chlorella Sp ◽  
Materials Used ◽  
Marine Exploration

Chlorella sp. merupakan sumber makanan bagi ikan dan udang. Untuk menumbuhkan, Chlorella sp. maka diperlukan media kultur dengan nutrien yang baik. Eucheuma sp. merupakan rumput laut yang mengandung mineral - mineral yang bermanfaat bagi pertumbuhan Chlorella sp. Pemberian ekstrak Eucheuma sp. diharapkan dapat mendukung pertumbuhan Chlorella sp. Tujuan dilakukan penelitian untuk mengetahui pengaruh ekstrak Eucheuma sp. terhadap kepadatan Chlorella sp. dan konsentrasi terbaik dari ekstrak Eucheuma sp. yang menghasilkan kepadatan Chlorella sp. yang tertinggi. Penelitian ini dilaksanakan pada bulan Januari - Maret 2005 di Laboratorium Bioteknologi dan Eksplorasi Laut, Teluk Awur, UNDIP, Jepara dan Laboratorium Jurusan Perikanan, UNDIP, Semarang. Materi yang digunakan yaitu ekstrak Eucheuma sp., Chlorella sp. dan media kultur. Metode yang digunakan adalah eksperimental laboratories. Konsentrasi ekstrak Eucheuma sp. yang digunakan adalah : A (10 mg/L); B (100 mg/L); C (200 mg/L); D (300 mg/L); E (400 mg/L); F (500 mg/L); G ( tanpa pemberian ekstrak Eucheuma sp.).Hasil penelitian menunjukkan perlakuan D (300 mg/L) menghasilkan kepadatan Chlorella sp. tertinggi pada puncak populasi, yaitu 6,293 log sel/mL dan pada akhir populasi, yaitu 6,012 log sel/mL dengan konstanta pertumbuhan spesifik sebesar 0,161.Kata kunci: Kepadatan, Chlorella, EucheumaChlorella sp. is the source of food to prawn and fish. In order to grow Chlorella sp. so need a culture media with a good nutrient. Eucheuma sp. is a seaweed it cantains benefit minerals for the growth of Chlorella sp. The giving of Eucheuma sp. it is to be hoped to support the the growth of Chlorella sp. This research is aimed to know the effect of Eucheuma sp. extract to density of Chlorella sp. and the best concentration of Eucheuma sp. extract produce the highest density of Chlorella sp. This research was conducted in January – March 2005 at the Laboratory of Biotechnology and Marine Exploration, Teluk Awur, UNDIP, Jepara and Laboratory of Fisheries Department UNDIP, Semarang. Materials used in this research consisted of Eucheuma sp. extract, Chlorella sp. and culture media. This research method used experimental laboratories. The concentration that used in this research : A (10 mg/L); B (100 mg/L); C (200 mg/L); D (300 mg/L); E (400 mg/L); F (500 mg/L); G ( without giving extract of Eucheuma sp.). The result of this research showed that the D treatment produce the highest Chlorella sp. density at peak of population, that is 6,293 log sel/mL and the end of population that is 6,012 log sel/mL with specific growth rate 0,161.Keyword : Density, Chlorella., Eucheuma

scholarly journals Optimization of Culture Conditions on Growth of Chlorella sp. Newly Isolated From Bagansiapiapi Waters Indonesia

2021 ◽  
Vol 934 (1) ◽  
pp. 012097
Author(s):  
D Iriani ◽  
B Hasan ◽  
HS Putra ◽  
TM Ghazali
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Cell Density ◽  
Specific Growth ◽  
Pharmaceutical Products ◽  
Culture Conditions ◽  
Marine Waters ◽  
Chlorella Sp ◽  
Need To Evaluate ◽  
Further Development

Abstract Chlorella sp. is a type of micro-sized green algae obtained in fresh or marine waters. However, many factors need to evaluate the growth of Chlorella sp. mainly the culture conditions. This study aimed to evaluate the culture conditions in optimizing the growth of Chlorella sp. newly isolated from Bagansiapiapi marine waters. The experiment was carried out at temperature ±25°C, light intensity 2300 Lux using TL-D lamp (36 W). There were six treatments as culture conditions for the cultivation of Chlorella sp.:1). Bean Sprouts (Vigna radiata) Extract Media (BSEM) with light continuously, 2. BSEM with a Photoperiod light:dark (10:14h), 3. Tofu waste media with light continuously, 4. Tofu waste media with a photoperiod light:dark (10: 14h), 5. Fish pellet solutions media (25%), 6. Fish pellet solutions media (50%). The parameter observed was the growth, cell density and the specific growth rate of Chlorella sp. every 2 days for 14 days of cultivation. Based on the results shown that the marine Chlorella sp. from Bagansiapiapi waters could be adapted to all treatments. Nevertheless, the use of BSEM light continuously obtained the highest cell density (27.75 x 105 cell/ml) on 12 days and continues to increase until 14 days, while the other treatments had decreased. Furthermore, the Chlorella sp. obtained the specific growth rate was 0.42. Therefore, the marine Chlorella sp. from Bagansiapiapi could be cultivated in BSEM light continuously for further development as a functional food or bioactive source in pharmaceutical products.

Specific growth rate of Chlamydomonas reinhardtii and Chlorella sorokiniana under medium duration light/dark cycles: 13–87 s

1999 ◽  
Vol 70 (1-3) ◽  
pp. 323-333 ◽  
Author(s):  
Marcel Janssen ◽  
Tjibbe Chris Kuijpers ◽  
Bram Veldhoen ◽  
Michel Brik Ternbach ◽  
Johannes Tramper ◽  
...  
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Chlamydomonas Reinhardtii ◽  
Specific Growth ◽  
Chlorella Sorokiniana

Specific growth rate and not cell density controls the general stress response in Escherichia coli

Microbiology ◽  
2004 ◽  
Vol 150 (6) ◽  
pp. 1637-1648 ◽  
Author(s):  
Julian Ihssen ◽  
Thomas Egli
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Stress Response ◽  
Specific Growth Rate ◽  
Cell Density ◽  
Specific Growth ◽  
Batch Cultures ◽  
General Stress Response ◽  
E Coli ◽  
Density Effects

In batch cultures of Escherichia coli, the intracellular concentration of the general stress response sigma factor RpoS typically increases during the transition from the exponential to the stationary growth phase. However, because this transition is accompanied by complex physico-chemical and biological changes, which signals predominantly elicit this induction is still the subject of debate. Careful design of the growth environment in chemostat and batch cultures allowed the separate study of individual factors affecting RpoS. Specific growth rate, and not cell density or the nature of the growth-limiting nutrient, controlled RpoS expression and RpoS-dependent hydroperoxidase activity. Furthermore, it was demonstrated that the standard E. coli minimal medium A (MMA) is not suitable for high-cell-density cultivation because it lacks trace elements. Previously reported cell-density effects in chemostat cultures of E. coli can be explained by a hidden, secondary nutrient limitation, which points to the importance of medium design and appropriate experimental set-up for studying cell-density effects.

Effect of a Freshwater Bacterium on Four Microcystis under Eutrophic Conditions

2013 ◽  
Vol 295-298 ◽  
pp. 1636-1642
Author(s):  
Wan Sheng Zou ◽  
Yu Shuang Luo ◽  
Liang Guo Liu ◽  
Wen Bin Wang ◽  
Pin Hong Yang
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Bacterial Strain ◽  
Specific Growth ◽  
Culture Media ◽  
Bacterial Flora ◽  
Cyanobacterial Blooms ◽  
Dynamic Changes ◽  
Microcystis Blooms ◽  
Cell Densities

The effect of a freshwater bacterium Exiguobacterium sp.013 was examined on the cultures of four freshwater Microcystis which can cause cyanobacterial blooms. Four freshwater Microcystis cells and the bacterium were inoculated simultaneously, in modified BG-11 culture media, and cultured for 24 days continuously. The specific growth rate and cell densities were employed and determined in treated cultures and in controls.Additionally, dynamic changes of bacterium were investigated during the whole cultures. The results showed that the specific growth rate and maximal cell density of M. aeruginosa, M. flos-aquae and M. wesenbergii in treated cultures were significantly enhanced than that of control cultures, and daily cell densities of four Microcystis in the treated cultures were also significantly higher compared with that of control cultures. The added bacterial strain always kept dominance among the bacterial flora during the culture period. These findings implied that bacteria Exiguobacterium sp. might play a positive promoting role in forming Microcystis blooms under the eutrophication condition.

scholarly journals Effect of Adding BIOM-S Probiotic on Survival and Growth Rate of Nile Tilapia (Oreochromis niloticus)

2018 ◽  
pp. 1-6
Author(s):  
Yuli Andriani ◽  
Taufik Ikhsan Kamil ◽  
Irfan Zidni ◽  
Iskandar . ◽  
Titin Herawati
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Specific Growth Rate ◽  
Nile Tilapia ◽  
Specific Growth ◽  
Culture Media ◽  
Control Treatment ◽  
Randomized Design ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Survival And Growth

This research was conducted in May-June 2017 in Hatchery Indoor Maksudi, Astanaanyar, Bandung. This research aims to find out the optimal BIOM-S probiotic on culture media of Nile tilapia, and the influence to survival rate and growth rate of Nile tilapia. This research was conducted by an experiment using Completely Randomized Design (CRD). This research consisted of five treatments and three replications, which were treatment A (control), treatment B (giving probiotic with 0,6 ml/L concentration), treatment C (giving probiotic with 0,8 ml/L concentration), treatment D (giving probiotic with ,0 ml/L concentration), and treatment E (giving probiotic with 1,2 ml/L concentration). The parameters in this research were the survival rate and specific growth rate. Survival rate and specific growth rate used data analysis with F test to find out the influence of each treatment. The concentration of optimal probiotic based on the results was 0.8 ml/L, it produced the highest survival rate for 81.67% and the specific growth rate for 0.039%.

scholarly journals Cultivation of Chlorella sp. as Biofuel Sources in Palm Oil Mill Effluent (POME)

2012 ◽  
Vol 1 (2) ◽  
pp. 45-49 ◽  
Author(s):  
H Hadiyanto ◽  
Muhamad Maulana Azimatun Nur ◽  
Ganang Dwi Hartanto
Keyword(s):  
Growth Rate ◽  
Renewable Energy ◽  
Specific Growth Rate ◽  
Nitrogen Source ◽  
Palm Oil ◽  
Specific Growth ◽  
Urea Concentration ◽  
Palm Oil Mill Effluent ◽  
Chlorella Sp ◽  
Palm Oil Mill

Renewable energy is essential and vital aspect for development in Indonesia especially less oil reserve for coming 15 years. Biodiesel has received much attention as renewable energy in recent years. One of potential biodiesel is produced from microalgae. Due to high content of nutrients in Palm Oil Mill Effluent (POME), this waste is a potential for nutrient growth for microalgae. Chlorella is one of high potential for biodiesel since it has high lipid content (20-30%). The objective of the research is to determine growth rate and biomass productivity in Chlorella Sp cultured in POME. Chlorella Sp was cultured in 20%, 50%, 70% POME using urea concentration 0.1gr/L (low nitrogen source) and 1gr/l (high nitrogen source) at flask disk, pH 6.8-7.2; aerated using aquarium pump and fluorescence lamp 3000-6000 lux as light. Medium was measured using spectrophotometer Optima Sp-300 OD at 680 wave length in 15 days to calculate specific growth rate. At end of cultivation, Chlorella sp was filtered and measured as dry weight. Result indicated that Chlorella sp at 50% POME 1gr/L urea showed higher specific growth rate (0.066/day). Factor affecting growth rate of microalgae is CNP ratio, POME concentration, and urea concentration.

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