scholarly journals The yeast culture Saccharomyces cerevisiae (Strain 47) as manipulator of rumen fermentation in postpartal period of dairy cows

Author(s):  
Petr Doležal ◽  
Jan Doležal

In the present study, examined was the effect of a yeast culture (Saccharomyces cerevisiae, Strain 47) on rumen fermentation of cows. Animals received a diet consisting of good maize silage with a higher dry matter content (16  kg), 16  kg of clovergrass haylage, 3  kg of meadow hay and 7.5  kg feed mixture. The yeast culture was added to the mixture in the dose 6  g/day and cow. The supplement of yeast culture showed a positive effect on VFA production in comparison with control (1.16±0.013B vs. 0.84±0.063A  g/ 100 ml), and lower production of lactic acid. The utilisation of ammonia was higher by cows in treated group (8.68±0.084A mmol/L). The difference in number of protozoa of cows in the control and experimental groups was significant (302.0±12.349A vs. 359.2±1.304B ths /1 ml of rumen fluid).

Author(s):  
Petr Doležal ◽  
Jan Dvořáček ◽  
Jitka Dvořáčková ◽  
Roman Poštulka ◽  
Jan Doležal ◽  
...  

The aim of this study was to determine the effect of a yeast culture (Saccharomyces cerevisiae CNCM I – 1077) addition in the diet of dairy cows on their rumen fermentation. Animals received a diet consisting of good maize silage with a higher dry matter content (18 kg), 6 kg of lucerne haylage, 5 kg of pea – silage, 3 kg of ensiled high moisture crimped corn, 1 kg of meadow hay, rape-cake 1 kg, ­brewer’s grain silage 4 kg, and 8.0 kg feed mixture. The yeast culture was added to the mixture in the dose 2.8*1010 cfu / day and cow. The supplement of yeast culture showed a positive effect on ruminal VFA production by experimental group cows in comparison with control, higher production (P < 0.01) of acetic acid (58.50 ± 1.2583b vs. 51.833 ± 2.409a %), and lower production of propionic and butyric acid (24.667 ± 1.3744a vs. 28.833 ± 1.863b %). The average utilisation of ammonia was higher by cows in treated group (12.267 ± 0.754 mmol / L), but difference was not significant. The difference in number of protozoa of cows in the control and experimental groups was significant (P < 0.05) different (308.333 ± 24.390a vs. 342.333 ± 10.9341b ths /1 ml of rumen fluid).


2011 ◽  
Vol 50 (No. 11) ◽  
pp. 503-510 ◽  
Author(s):  
P. Doležal ◽  
J. Doležal ◽  
J. Třináctý

This study presents the results of an experiment in which the effect of addition of a yeast culture (Saccharomyces cerevisiae, Strain 47) on rumen fermentation was studied in thirty-six dairy cows of Holstein breed.The animals were divided into one control and five experimental groups. Each group involved 6 individuals. The animals received a diet consisting of good maize silage with a higher dry matter content (16 kg), clover-grass haylage (l6 kg), meadow hay (3 kg) and supplementary feed mixture (7.5 kg). The rations were fed to cows as total mixed ration (TMR).In experimental groups, the yeast culture was added into the feed mixture in amounts of 2, 4, 6, 8, 10 g per day and animal. Samples of rumen fluid were taken perorally 3&ndash;4 hours after feeding. The obtained results indicated that the addition of a Saccharomyces cerevisiae SC-47 culture in recommended doses showed a positive effect on ruminal digestion. As compared with control, the addition of all aforementioned amounts of the yeast culture into the feeding ration resulted in all cases in a statistically significant (P &lt; 0.01) decrease in pH and fluctuated near the lower limit of the reference values. As compared with control, the yeast culture supplementation showed a positive effect (P &lt; 0.01) on production of volatile fatty acids (VFA) (127.6 vs. 84.0 mmol/l). The utilisation of ammonia was higher (P &lt; 0.01) in experimental groups (8.12, resp. 8.68 mmol/l) than in controls (9.06 mmol/l). The difference in protozoa numbers in rumens of dairy cows in the control and experimental groups was statistically highly significantly (P &lt; 0.01) different. There was a close relationship between the dose of yeast culture on the one hand and the VFA content and protozoa numbers on the other. The regression analysis of dependence of dependent variable (i.e. pH of rumen fluid) on the independent one (i.e. the dose of yeast culture) revealed only a slight degree of dependence (r = 0.671). &nbsp;


2020 ◽  
Vol 44 (5) ◽  
pp. 1110-1114
Author(s):  
Serkan ÖZKAYA ◽  
Ozan Emre ALMALI ◽  
Yavuz AYAN ◽  
Sabri ERBAŞ ◽  
Taylan AKSU

The study aimed to determine the effects of the addition of aromatic oregano water (AOW) on rumen fermentation of Holstein calves. For this purpose, 20 Holstein calves were divided into four groups (n = 5) and fed with three different doses of AOW (1, 1.5, and 2%). The experimental groups were formed as follows; the control group (CNT): milk + starter; G1: 1% AOW supplemented milk + starter; G2: 1.5% AOW supplemented milk + starter; G3: 2% AOW supplemented milk + starter. The rumen fluid was taken from the oesophagus at 60 days of age with the aid of a rumen probe. In acetic acid (AA), propionic acid (PA), and butyric acid (BA) except for total volatile fatty acid (TVFA) (P < 0.05), the numerical differences between the means of the groups were not found statistically significant. The rumen pH values of the groups were not affected by the addition of AOW. Likewise, the difference between the total bacteria and the numbers of protozoa, Entodinum, Diplodinium, Isotrichia, and Daystrichia were not statistically significant. The results of the study showed that the addition of AOW did not have a negative or positive effect on rumen fermentation of the calves.


Author(s):  
S.M. Elhassan ◽  
R.J. Wallace ◽  
C.J. Newbold ◽  
X.B. Chen ◽  
I.E. Edwards ◽  
...  

Yeast culture (YC) based on Saccharomyces cerevisiae is gaining increasing acceptance as a feed additive for ruminants. Production responses to YC have been reported in growing and lactating animals. It has been suggested that these responses are at least partly due to enhanced microbial growth in and microbial nitrogen flow from the rumen (Williams and Newbold, 1990). The aim of the present study was to investigate the effect of YC on rumen fermentation, microbial growth and liveweight gain in bulls fed isonitrogenous diets containing either urea or soya.Thirty two Limousin cross Friesian bulls (liveweight 344 kg) were allocated to treatments on the basis of previous growth rate and initial liveweight. Animals received mixed diets of barley/urea (92% rolled barley, 5% cane molasses, 1.5% urea and 1.5% minerals/vitamin mix) or barley/soya (86.75% rolled barley, 5% cane molasses, 0.75% urea, 6% soyabean meal and 1.5% minerals/vitamin mix) plus or minus 1.5 kg/ tonne YC (Yea-sacc1026 , Alltech, UK) (Table 1). YC was added to the diet prior to mixing. All rations were offered ad libitum twice daily until slaughter (460 kg). Rumen fluid samples were withdrawn by stomach tube 5 and 10 weeks after the beginning of the trial. The outflow of microbial nitrogen from the rumen was estimated from the appearance of purine derivatives in the urine as described previously (Chen et al., 1990).


Author(s):  
C.J. Newbold ◽  
R.J. Wallace ◽  
I.M. Nevison

A wide range of compounds has been described which have the potential to improve animal production by manipulating the rumen fermentation. Prominent among these rumen modifiers are the ionophores. Ionophores, such as monensin and tetronasin, improve feed efficiency, partly by increasing the flow of amino-N from the rumen and partly by stimulating the production of propionate in the rumen with an associated reduction in the production of methane (Russell and Strobel, 1988). Recently there has been increasing interest in the use of yeast culture (YC) and other fungal preparation to modify the rumen fermentation. These products have been shown to increase bacterial numbers within the rumen with an associated increase in the breakdown of fibre and supply of microbial protein (Williams and Newbold, 1990). YC has also been reported to increase the production of propionate in the rumen. Little appears to be known about the effect a combination YC and an ionophore would have on the rumen fermentation. This study describes the effects of the ionophores monensin and tetronasin on the fermentation of hay by rumen fluid from sheep fed a basal diet with or without YC.


2008 ◽  
Vol 52 (No. 12) ◽  
pp. 423-429 ◽  
Author(s):  
S. Kinal ◽  
A. Korniewicz ◽  
M. Słupczyńska ◽  
R. Bodarski ◽  
D. Korniewicz ◽  
...  

The object of an experiment was inorganic and organic forms of zinc, copper and manganese applied in mineral mixtures to dairy cows. The experiment was carried out on 90 cows with average milk yield for previous lactation of about 9 500 kg milk. The cows received mineral mixtures containing inorganic or organic forms of zinc, copper and manganese for 6 weeks before calving and during the first three months of lactation. The application of microelements as bioplexes in amounts covering 30% of daily requirements of cows had a positive effect on an increase in colostrum dry matter content from 20.9 to 23.35% as well as on the concentration of protein and fat, and the level of minerals – calcium, phosphorus, magnesium, zinc and copper. In cows’ milk in the 2<sup>nd</sup> and 3<sup>rd</sup> month of lactation there were no differences in contents of minerals. However in the blood serum of cows in the 1<sup>st</sup> and 2<sup>nd</sup> month of lactation an increase in calcium concentration from 1.96 to 2.14 g/kg was observed while the content of phosphorus also increased average from 1.76 to 2.22 g/kg in the first trimester of lactation.


Author(s):  
P.P. Frumholtz ◽  
C.J. Newbold ◽  
E.R. Ørskov ◽  
R.J. Wallace

Monensin and yeast culture are known to alter rumen fermentation. Their modes of action appear to be different, and a recent study showed that the combination of both yeast culture (YC) and monensin stimulated weight gain in bulls by 12-13% over monensin alone (Spedding, 1990). However, Edwards et al. (1991) found no complementary effects between YC and avoparcin when they were combined in a silage diet for steers. In addition to its action in the rumen, monensin is known to affect post-ruminal digestion (Armstrong, 1984). Viable Saccharomyces cerevisiae have been isolated from the duodenal digesta of sheep fed YC (Newbold et al., 1990), suggesting that YC too might have post-ruminal effects. The aim of the current study was to assess the effect of monensin and/or yeast culture in the diet or infused into the abomasum on rumen fermentation and blood parameters, and also on digestive physiology by monitoring plasma gastrin, insulin and CCK-8 concentration.


2013 ◽  
Vol 27 (2) ◽  
pp. 195-201 ◽  
Author(s):  
U. Prośba-Białczyk ◽  
H. Szajsner ◽  
E. Grzyś ◽  
A. Demczuk ◽  
E. Sacała ◽  
...  

Abstract Germination and sugar beet yield after seed stimulation were investigated. The seeds came from the energ'hill technology and were subject to laser irradiation. The experiments were conducted in the laboratory and field conditions. Lengthening of germinal roots and hypocotyls was observed. A positive effect of the stimulation on the morphological features was observed for the Eh seeds and laser irradiation applied in a three-fold dose. The energ'hill seeds exhibited a significantly higher content of carotenoids in seedlings and an increase in the content of chlorophylls. Laser light irradiation favourably modified the ratio of chlorophyll a to b. The leaves and roots of plants developed from the energ'hill and irradiated seeds were characterized by higher dry matter content thanin non-stimulated seeds. Seed stimulation had a positive influence on yielding and the saccharose content.


Author(s):  
Sabarta Sembiring ◽  
Pratiwi Trisunuwati ◽  
Osfar Sjofjan ◽  
Irfan Djunaidi

This study evaluated the nutritional and tannin contents of cooked Kepok banana corm, fermented with Saccharomyces cerevisiae and Aspergillus niger. Corm meal was pretreated by steam for 1 hour at 102 0C before fermenting. The pretreated corms were inoculated with 10% (w/v) inoculum dose with additional nutrient mono-culture or co-culture. A completely randomized design with a 3 x 3 factorial arrangement was used to investigate two main factors: microbial strains and incubation time, with three subfactors each, resulting in nine treatment combinations and three replications. The microbial strain and incubation time significantly (P>0.01) affected the nutrient content of fermented Kepok banana corms. The highest dry matter content (91.84%) was achieved by incubation with S. cerevisiae for 48 h, while 89.61% organic matter was obtained by fermentation with both S. cerevisiae + A. niger for 48 h. The highest crude protein content was 5.81%, which resulted from treatment with S. cerevisiae for 96 h, increasing the crude protein by 61% compared to the control (3.58% crude protein). Fermentation by the microbe consortium (S. cerevisiae + A. niger) for 72 h, produced maximum starch (35.54 g/100 g) and energy (3511 Kcal/kg) values. Thus, the fermented products are a potential source of energy, to be used as feed ingredients.


1946 ◽  
Vol 36 (3) ◽  
pp. 207-213 ◽  
Author(s):  
H. G. Wager

The dry-matter content of about 260 samples of potatoes was determined. The samples were collected over three seasons and from many types of soil.The average dry-matter content of a variety varied from season to season, but it always bore an approximately constant relationship to the average value of other varieties.Wet seasons led to potatoes low in dry matter.The variation in the content of dry matter of potatoes in different seasons and from different soil types is not a direct effect of the water balance of the tubers.The average content of dry matter of potatoes depends on the soil in which they were grown; fen and blackland gave potatoes with the lowest dry matter, followed by skirt, silt and warp, then loam and medium loam, then clay, and the highest dry matter occurred in stocks grown in sands, gravels or light loams.The difference between the soils is discussed, and it is tentatively concluded that the factor responsible for the variation in content of dry matter of the potatoes is the available water content of the soils.


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