scholarly journals Replacing Alfalfa with Paper Mulberry in Total Mixed Ration Silages: Effects on Ensiling Characteristics, Protein Degradation, and In Vitro Digestibility

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1273
Author(s):  
Rongrong Li ◽  
Mingli Zheng ◽  
Di Jiang ◽  
Pengjiao Tian ◽  
Menghu Zheng ◽  
...  
Keyword(s):  
Protein Degradation ◽  
Crude Protein ◽  
Dry Matter ◽  
Condensed Tannin ◽  
Protein Digestibility ◽  
In Vitro Digestibility ◽  
Protein Nitrogen ◽  
Total Mixed Ration ◽  
Fermentation Quality

To develop an alternative high-protein forage resource to alleviate ruminant feed shortages, we investigated the effects of replacing alfalfa (Medicago sativa L.) with different ratios of paper mulberry (Broussonetia papyrifera L., RY) on fermentation quality, protein degradation, and in vitro digestibility of total mixed ration (TMR) silage. The TMR were made with alfalfa and RY mixtures (36.0%), maize meal (35.0%), oat grass (10.0%), soybean meal (7.5%), brewers’ grain (5.0%), wheat bran (5.0%), premix (1.0%), and salt (0.5%) on a dry matter basis, respectively. The alfalfa and RY mixtures were made in the following ratios of dry matter: 36:0 (RY0), 27:9 (RY9), 18:18 (RY18), 9:27 (RY27), and 0:36 (RY36). After ensiling for 7, 14, 28, and 56 days, fermentation quality, protein degradation, and microbial counts were examined, and chemical composition and in vitro digestibility were analyzed after 56 days of ensiling. All TMR silages, irrespective of the substitution level of RY, were well preserved with low pH and ammonia nitrogen content, high lactic acid content, and undetectable butyric acid. After ensiling, the condensed tannin content for RY18 silages was higher than the control, but non-protein nitrogen, peptide nitrogen, and free amino acid nitrogen contents was lower, while the fraction B1 (buffer-soluble protein) was not different among all the silages. Dry matter and crude protein digestibility for RY27 and RY36 silages was lower than the control, but there was no difference between control and RY18 silages. This study suggested that ensiling RY with alfalfa inhibited true protein degradation, but decreased in vitro dry matter and crude protein digestibility of TMR silages, and that 18:18 is the optimal ratio.

scholarly journals Crude protein, fibre and phytic acid in vitro digestibility of selected legume and buckwheat samples

2013 ◽  
Vol 61 (1) ◽  
pp. 227-232 ◽  
Author(s):  
Petra Vojtíšková ◽  
Stanislav Kráčmar
Keyword(s):  
Phytic Acid ◽  
Crude Protein ◽  
Dry Matter ◽  
Protein Digestibility ◽  
The Other ◽  
In Vitro Digestibility ◽  
Common Beans ◽  
Commission Regulation ◽  
Phytate Content

The aim of this study was to determine crude protein, fibre and phytic acid in vitro digestibility of selected legumes and buckwheat products. All analyses except the phytic acid contents were performed in the line with the Commission Regulation (EC) No. 152/2009. A modified version of Holt’s Method was used for phytic acid (phytate) determination. None of all samples contained more than 11% of moisture. Soybeans are rich in crude protein; they contain nearly 40% of this compound. The content of crude protein in buckwheat flours was about 14%. The highest amount of phytate was found in common beans and soybeans-about 2 g/100 g of dry matter. On the other hand, the lowest phytate content was observed in buckwheat pasta (< 1 g/100 g). The quantification of phytate in F. esculentum groats was 1.9 g per 100 g of dry matter. In vitro digestibility was determined using an incubator Daisy and pepsin enzymes and the combination of pepsin and pancreatin. The highest coefficient of crude protein digestibility was discovered to be in peels and wholemeal flour. The greatest fibre digestibility coefficients were obtained for peels, which contain about 65% of fibre in their dry matter. When pepsin was used, a higher phytic acid digestibility coefficient for G. max, Ph. vulgaris, peels, flour, groats and broken groats was observed; while when the combination of pepsin and pancreatin was used, higher phytic acid digestibility coefficients for peas, lentil and wholemeal flour were observed.

scholarly journals High Fiber Cakes from Mediterranean Multipurpose Oilseeds as Protein Sources for Ruminants

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 918 ◽  
Author(s):  
Francesco Serrapica ◽  
Felicia Masucci ◽  
Emiliano Raffrenato ◽  
Maura Sannino ◽  
Alessandro Vastolo ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Protein Digestibility ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Ether Extract ◽  
Protein Supplements ◽  
High Fiber ◽  
Alternative Protein

Fifteen oilseed cakes from sunflower, pomegranate, cardoon, tobacco and hemp were characterized with regard to chemical composition, Cornell Net Carbohydrate and Protein System (CNCPS) fractionation, in vitro digestibility of dry matter, neutral detergent fiber, and crude protein. All the cakes presented low moisture, rather variable ether extract contents and medium to high levels of crude protein and neutral detergent fiber. The cakes significantly differed in terms of CNCPS partitioning and in vitro digestibility. Tobacco and hemp cakes presented high contents of slow degradable fractions of crude protein and carbohydrate joined to good post-ruminal protein digestibility. Cardoon cakes presented the highest rumen protein degradability. Based on crude protein content and intestinal digestibility of rumen undegraded protein, cakes of tobacco and hemp showed the better potential as alternative protein supplements for ruminants, while pomegranate appears to be the least suitable for ruminant feeding.

scholarly journals PSXI-35 Effects of cultivation stage and supplementation level of sprouted barley on nutrient content and fermentation quality of fermented diet

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 402-403
Author(s):  
Hyuk jun lee ◽  
Young Ho Joo ◽  
Seong Shin Lee ◽  
Jong Ho Park ◽  
Tae Il Kim ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Nutrient Content ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Total Mixed Ration ◽  
Microbial Counts ◽  
Fermentation Quality

Abstract This study was aimed to estimate the effect of cultivation stage and supplementation level of sprouted barley (SB) on nutrient content and fermentation quality of fermented total mixed ration (TMR). The SB were collected at day 6 (SB6) and day 8 (SB8) of cultivation stages and substituted at 5% (TMR5) and 10% (TMR10) of TMR on dry matter (DM) basis. These TMR were ensiled into 10 L mini silo (3 kg) for 14 days in triplicate. After silo open, sample (1 kg) was collected for laboratory analysis. Before ensiling, crude protein (CP) was lower (P = 0.012) in SB6 than in SB8. Additionally, DM in TMR10 was slightly lower (P = 0.015) than in TMR5 while CP and acid detergent fiber were higher (P &lt; 0.05). After fermentation, crude ash (P = 0.002), in vitro DM digestibility (IVDMD) (P = 0.001; 73.5 vs. 72.0%) and in vitro neutral detergent fiber (IVNDFD) (P = 0.001; 50.6 vs. 47.7%) were higher in SB6 than in SB8. Additionally, DM (P = 0.002; 64.0 vs. 64.5) in TMR10 was lower than in TMR5, while CP (P = 0.005; 19.7 vs. 18.9%), IVDMD (P = 0.001; 74.5 vs. 71.0%), and IVNDFD (P = 0.001; 52.0 vs. 46.3%) were higher. Fermentation indices and microbial counts were not affected by cultivation stage of SB, except on propionate that higher (P = 0.014) in SB8 than SB6. Silage pH (P = 0.001) was lower in TMR10 than in TMR5, which followed by higher lactate (P = 0.003; 3.27 vs. 2.85%), acetate (P = 0.004; 0.52 vs. 0.42%), propionate (P &lt; 0.001), and LAB count (P = 0.020; 7.49 vs. 7.19%). The interaction effects between cultivation stage and supplementation level only found on propionate production (P = 0.001). Yeast and mold were not affected by cultivation stage and supplementation level of SB. This study concluded SB6 was recommended to improve fermentation quality of fermented diet, and its application on TMR10 could increase not only rumen digestibility but also fermentation indices.

The assessment of herbage legume varieties. II. In vitro digestibility, water soluble carbohydrate, crude protein and mineral content of primary growth of clover and lucerne

1966 ◽  
Vol 66 (3) ◽  
pp. 351-357 ◽  
Author(s):  
W. Ellis Davies ◽  
G. ap Griffith ◽  
A. Ellington
Keyword(s):  
White Clover ◽  
Crude Protein ◽  
Dry Matter ◽  
Red Clover ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Soluble Carbohydrate ◽  
In Vitro Digestibility ◽  
Primary Growth

The primary growth of eight varieties of three species–white clover (3), red clover (4) and lucerne (1)–was sampled at fortnightly intervals and the percentage dry matter, in vitro digestibility, crude protein, water soluble carbohydrates, P, Ca, K, Na and Mg were determined.Differences between species were nearly always significant and the general order of merit was white clover, red clover and lucerne. The exceptions were for dry-matter percentage where this order was reversed, and red clover had the lowest Na and highest Mg content.

YIELD AND QUALITY OF SOME KALE-CORN COMBINATIONS

1980 ◽  
Vol 60 (3) ◽  
pp. 807-811 ◽  
Author(s):  
R. S. FULKERSON
Keyword(s):  
Protein Content ◽  
Corn Stover ◽  
Crude Protein ◽  
Dry Matter ◽  
In Vitro Digestibility ◽  
Crude Protein Content ◽  
Yield And Quality ◽  
Row Width

Midas marrowstem kale (Brassica oleracea L.) was grown in different row width associations with United 106 corn (Zea maize L.) in two studies and ensiled in different moisture blends with corn stover in another. Highest dry matter yields were obtained where a single row of kale was grown at 30 cm to the side of a corn row. This combination also provided the lowest moisture content feed and the highest in vitro digestibility and crude protein content. Changing the corn row width had no significant effect upon yield, plant height, in vitro digestibility, kale leaf or corn ear content. Blending kale with corn stover to provide a silage of about 70% moisture increased the digestibility and protein content of the feed and provided a silage that kept well in storage.

scholarly journals Sunflower cake in diets for beef cattle: digestibility, kinetics and in vitro ruminal fermentation parameters

2018 ◽  
Vol 41 (1) ◽  
pp. 39492 ◽  
Author(s):  
Rafael Henrique de Tonissi e Buschinelli de Goes ◽  
Luiz Henrique Xavier da Silva ◽  
Tatiana García Díaz ◽  
Antonio Ferriani Branco ◽  
Ana Lúcia Teodoro ◽  
...  
Keyword(s):  
Organic Matter ◽  
Beef Cattle ◽  
Soybean Meal ◽  
Crude Protein ◽  
Dry Matter ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Fermentation Products ◽  
Sunflower Cake

 The objective of this study was to evaluate the effect of the inclusion of sunflower cake replacing soybean meal in beef cattle diets on the in vitro digestibility of dry matter (IVDDM), organic matter (IVDOM), crude protein (IVDCP) and the ruminal fermentation kinetics and parameters. The experiment was analyzed according to a completely randomized design. The treatments consisted of four levels of sunflower cake, 0, 200, 400, 600 g kg-1, replacing soybean meal in the concentrate of beef cattle diets. The coefficients of IVDDM, IVDOM and IVDCP presented a quadratic effect with the addition of sunflower cake. The soluble fraction (fraction B) degradation rate and total gas production decreased linearly with the inclusion of sunflower cake. Values of pH in ruminal fluid were higher for levels 0, 200 and 600 g kg-1 sunflower cake. Sunflower cake can replace soybean meal by up to 280 g kg-1 in the concentrate of beef cattle diets, improving the in vitro digestibility of dry matter organic, matter and crude protein. Levels above 400 g kg-1 reduce ruminal digestion rate, digestibility and release of final fermentation products. 

scholarly journals Utilization of a novel feedstuff: effects of additives on the fermentation characteristics, chemical composition and in vitro digestibility of tetraploid black locust (Robinia pseudoacacia) silage

2019 ◽  
Vol 49 (7) ◽  
Author(s):  
Zhihao Dong ◽  
Junfeng Li ◽  
Lei Chen ◽  
Siran Wang ◽  
Tao Shao
Keyword(s):  
Lactic Acid ◽  
Chemical Composition ◽  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Fermentation Products ◽  
Fermentation Quality ◽  
Tetraploid Black Locust

ABSTRACT: This study was conducted to evaluate the effects of additives on the fermentation characteristics, chemical composition and in vitro digestibility of tetraploid black locust (TBL). The TBL leaves silage was either untreated (control) or treated with 1 × 106 cfu/g FM Lactobacillus plantarum (L), 1% glucose (G), 3% molasses (M), a combination of 1% glucose and Lactobacillus plantarum (L+G), or a combination of 3% molasses and Lactobacillus plantarum (L+M). Fermentation quality, chemical composition and nutrient digestibility were then analyzed. Ethanol and acetic acid concentrations were the dominant fermentation products in all silages except L+M silage. The L, G and L+G treatments failed to influence the fermentation. The M treatment increased (P<0.05) the lactic acid concentration and lowered (P<0.05) the pH when compared with control silage. The best fermentation properties were observed in L+M silage, as indicated by the dominance of lactic acid over ethanol in fermentation products. The M and L+M silages exhibited higher (P<0.05) dry matter, and M silage showed higher residual water-soluble carbohydrates than the control. Ensiling increased (P<0.05) the in vitro dry matter, neutral detergent fiber and acid detergent fiber degradability of TBL. Among the silages, M silage had the highest levels of dry matter, neutral detergent fiber and acid detergent fiber degradability. The obtained results suggested that application of lactic acid bacteria together with 3% molasses could be an effective strategy to prevent the occurrence of ethanol fermentation and improve fermentation quality of TBL silage; addition of fermentable sugars to TBL improves nutrient availability to ruminants.

EVALUATION OF PINEGRASS AS RANGE FORAGE

1969 ◽  
Vol 49 (3) ◽  
pp. 351-359 ◽  
Author(s):  
Alastair McLean ◽  
S. Freyman ◽  
J. E. Miltimore ◽  
D. M. Bowden
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Soluble Carbohydrate ◽  
In Vitro Digestibility ◽  
Native Forest ◽  
Acid Detergent Fiber ◽  
High Positive Correlation ◽  
Calcium Iron

Seasonal changes in the quality of pinegrass (Calamagrostis rubescens) on native forest range in southern British Columbia included declines in crude protein content and in vitro digestibility of dry matter and increases in content of lignin, acid-detergent fiber, and ash. The decline in average daily gains of beef heifers was more rapid than changes in quality of the pinegrass over the grazing period from June to October.Pinegrass contained adequate nutrients for the rapid growth of yearlings or for maintaining weanling calves until August 1, whereupon protein and phosphorus supplementation were required. Nutrient requirements of pregnant cows and replacement heifers were adequately met until September 1.Copper and zinc were inadequate throughout the season. Calcium, iron, and manganese contents, however, were satisfactory. Silica was high and must be considered a potential problem in pinegrass utilization.Digestible dry matter gave a high positive correlation with crude protein and phosphorus and a high negative correlation with lignin, acid-detergent fiber, and total ash.Crude fiber, crude fat, and total soluble carbohydrate contents of pinegrass did not closely follow changes in either animal gains or in vitro digestible dry matter.Crude protein, phosphorus, and acid-detergent fiber appear to give a good indication of animal yields on pinegrass and could be used to evaluate pinegrass quality where animal data are not available.

Fermentation quality, in vitro digestibility and aerobic stability of total mixed ration silages prepared with whole-plant corn (Zea mays L.) and hulless barley (Hordeum vulgare L.) straw

2018 ◽  
Vol 58 (10) ◽  
pp. 1860 ◽  
Author(s):  
XianJun Yuan ◽  
AiYou Wen ◽  
Jian Wang ◽  
JunFeng Li ◽  
Seare T. Desta ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Ammonia Nitrogen ◽  
Stability Test ◽  
Small Scale ◽  
In Vitro Digestibility ◽  
Total Mixed Ration ◽  
Aerobic Stability ◽  
Fermentation Quality

This study was carried out to assess the effects of adding Lactobacillus plantarum, molasses or/and ethanol on the fermentation quality, in vitro digestibility and aerobic stability of total mixed ration (TMR) silage, which is well accepted in small-scale dairy farms in Tibet. Total mixed ration were ensiled in laboratory silos (1 L) and treated with (1) no additive (Control), (2) ethanol (E, 25 ml/kg fresh weight (FW)), (3) molasses (M, 30 g/kg FW); (4) Lactobacillus plantarum (L, 106cfu/g FW); (5) ethanol + molasses (EM); and (6) ethanol + Lactobacillus plantarum (EL). After 45 days of ensiling, six silos per treatment were opened for the fermentation quality and in vitro digestibility analyses, whereas 18 silos were used for the aerobic stability test for the following 9 days. All TMR silages were well preserved with dominant lactic acid (LA), low pH and ammonia nitrogen, and negligible propionic and butyric acid. The L and EL silages had the lowest pH and highest LA concentrations. The addition of ethanol did not inhibit silage fermentation as there were no significant differences for the pH, LA, acetic acid, negligible propionic acid or ammonia nitrogen content, lactic acid bacteria and yeast counts between Control and the E silage. During the aerobic stability test, pH increased by 1.39, 1.67, 1.69 and 0.74 for the Control, M, L and EM silages, but only 0.40 and 0.34 for E and EL silages, respectively. Upon exposure to air, the LA concentration in the L silage was evidently (P < 0.05) decreased, whereas LA concentration in the EL silage remained the highest value after the third day of aerobic exposure. Mean populations of aerobic bacteria and yeast in the E and EL silages were lower (P < 0.05) than those of the Control. These findings suggested that L. plantarum is effective in improving fermentation quality of TMR silages. Although the addition of ethanol in our study did not depress the fermentation of the TMR silages, it showed potential to inhibit the aerobic spoilage of TMR silages, either alone or in combination with the L. plantarum. It is concluded that L. plantarum combined with ethanol not only ensures better fermentation but also could improve aerobic stability.

scholarly journals Estimation of in vivo digestibility with the laying hen by an in vitro method using the intestinal fluid of the pig

1980 ◽  
Vol 43 (2) ◽  
pp. 389-391 ◽  
Author(s):  
K. Sakamoto ◽  
T. Asano ◽  
S. Furuya ◽  
S. Takahashi
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Laying Hen ◽  
Intestinal Fluid ◽  
Protein Nitrogen ◽  
In Vitro Method ◽  
Poultry Diets

1. Dry matter and crude protein (nitrogen × 6.25) digestibility of four poultry diets determined by an in vitro method using the intestinal fluid of pigs was significantly correlated with corresponding in vivo digestibility values obtained with hens.2. The intestinal fluid could be lyophilized and stored for at least 35 d without losing its activity on digestion.

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