Rubidium influx into rat skeletal muscles in relation to electrical activity

In this chapter, the monitoring of the electrical activity of skeletal muscles is depicted. The main components of the detection and conditioning of the EMG signals is explained in the sense of the biomedical instrumentation. But, first, a brief description of EMG generation is introduced. The hardware components of the general instrumentation system used in the acquisition of EMG signal such as amplifier, filters, analog-to-digital converter are discussed in detail. Subsequently, different types of electrodes used in different EMG techniques are mentioned. Then, various EMG signals that can be detected and monitored via EMG systems are described and their clinical importance is discussed with detail. Finally, different EMG techniques used in clinical studies and their purposes are explained with detail.

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The ups and downs of gene regulation by electrical activity in skeletal muscles

Journal of Muscle Research and Cell Motility ◽

10.1007/s10974-010-9200-2 ◽

2009 ◽

Vol 30 (7-8) ◽

pp. 255-260 ◽

Cited By ~ 12

Author(s):

Zaheer A. Rana ◽

Kristian Gundersen ◽

Andres Buonanno

Keyword(s):

Gene Regulation ◽

Electrical Activity ◽

Skeletal Muscles

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Clinical Features and Management of Some Muscular Disorders in Children - A Brief Review

Paediatrica Indonesiana ◽

10.14238/pi38.11-12.1998.233-42 ◽

2017 ◽

Vol 38 (11-12) ◽

pp. 233

Author(s):

Dwi Putro Widodo

Keyword(s):

Differential Diagnosis ◽

Peripheral Nerve ◽

Electrical Activity ◽

Nerve Conduction ◽

Skeletal Muscles ◽

Nerve Impulse ◽

Nerve Conduction Studies ◽

Muscle Disease ◽

Diagnostic Tools ◽

Muscular Disorders

Study has proven that electrical activity of resting and contracting skeletal muscles and conduction of the nerve impulse have become one of the most useful diagnostic tools in neurology. EMG and nerve conduction studies have become indispensable in the diagnosis and management of peripheral nerve and muscle disease (neurological differential diagnosis).

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Detection and Conditioning of EMG

Advances in Medical Technologies and Clinical Practice - Applications, Challenges, and Advancements in Electromyography Signal Processing ◽

10.4018/978-1-4666-6090-8.ch003 ◽

2014 ◽

pp. 58-94

Author(s):

İmran Göker

Keyword(s):

Electrical Activity ◽

Clinical Studies ◽

Skeletal Muscles ◽

Clinical Importance ◽

Analog To Digital Converter ◽

Digital Converter ◽

Analog To Digital ◽

Emg Signal ◽

Different Types ◽

Main Components

In this chapter, the monitoring of the electrical activity of skeletal muscles is depicted. The main components of the detection and conditioning of the EMG signals is explained in the sense of the biomedical instrumentation. But, first, a brief description of EMG generation is introduced. The hardware components of the general instrumentation system used in the acquisition of EMG signal such as amplifier, filters, analog-to-digital converter are discussed in detail. Subsequently, different types of electrodes used in different EMG techniques are mentioned. Then, various EMG signals that can be detected and monitored via EMG systems are described and their clinical importance is discussed with detail. Finally, different EMG techniques used in clinical studies and their purposes are explained with detail.

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Distribution of c-protein isoforms in sarcomeres of developing chick skeletal muscle

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010010319x ◽

1988 ◽

Vol 46 ◽

pp. 226-227

Author(s):

D. A. Fischman ◽

J. E. Dennis ◽

T. Obinata ◽

H. Takano-Ohmuro

Keyword(s):

Skeletal Muscles ◽

Thick Filament ◽

Protein Isoforms ◽

Actin Binding ◽

Striated Muscles ◽

C Protein ◽

Sarcomeric Protein ◽

Thick Filaments ◽

Cross Bridges ◽

Bridge Bearing

C-protein is a 150 kDa protein found within the A bands of all vertebrate cross-striated muscles. By immunoelectron microscopy, it has been demonstrated that C-protein is distributed along a series of 7-9 transverse stripes in the medial, cross-bridge bearing zone of each A band. This zone is now termed the C-zone of the sarcomere. Interest in this protein has been sparked by its striking distribution in the sarcomere: the transverse repeat between C-protein stripes is 43 nm, almost exactly 3 times the 14.3 nm axial repeat of myosin cross-bridges along the thick filaments. The precise packing of C-protein in the thick filament is still unknown. It is the only sarcomeric protein which binds to both myosin and actin, and the actin-binding is Ca-sensitive. In cardiac and slow, but not fast, skeletal muscles C-protein is phosphorylated. Amino acid composition suggests a protein of little or no αhelical content. Variant forms (isoforms) of C-protein have been identified in cardiac, slow and embryonic muscles.

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Measurement of spontaneous neuronal membrane activity by time lapse confocal microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100141238 ◽

1995 ◽

Vol 53 ◽

pp. 972-973

Author(s):

R H. Selinfreund ◽

A. H. Cornell-Bell

Keyword(s):

Membrane Potential ◽

Confocal Microscopy ◽

Patch Clamp ◽

Electrical Activity ◽

Time Lapse ◽

Neuronal Firing ◽

Neuronal Membrane ◽

Pituitary Cells ◽

Patch Clamp Recording ◽

Spontaneous Electrical Activity

Cellular electrophysiological properties are normally monitored by standard patch clamp techniques . The combination of membrane potential dyes with time-lapse laser confocal microscopy provides a more direct, least destructive rapid method for monitoring changes in neuronal electrical activity. Using membrane potential dyes we found that spontaneous action potential firing can be detected using time-lapse confocal microscopy. Initially, patch clamp recording techniques were used to verify spontaneous electrical activity in GH4\C1 pituitary cells. It was found that serum depleted cells had reduced spontaneous electrical activity. Brief exposure to the serum derived growth factor, IGF-1, reconstituted electrical activity. We have examined the possibility of developing a rapid fluorescent assay to measure neuronal activity using membrane potential dyes. This neuronal regeneration assay has been adapted to run on a confocal microscope. Quantitative fluorescence is then used to measure a compounds ability to regenerate neuronal firing.The membrane potential dye di-8-ANEPPS was selected for these experiments. Di-8- ANEPPS is internalized slowly, has a high signal to noise ratio (40:1), has a linear fluorescent response to change in voltage.

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The effect of ionophore A23178 on the α-actinin crosslinks in the z-band of frog skeletal muscle: An EM study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100142396 ◽

1986 ◽

Vol 44 ◽

pp. 154-155

Author(s):

F.T. Llados ◽

V. Krlho ◽

G.D. Pappas

Keyword(s):

Muscle Damage ◽

Transmitter Release ◽

Skeletal Muscles ◽

Calcium Uptake ◽

Muscle Membrane ◽

Frog Skeletal Muscle ◽

Ach Release ◽

Sustained Action ◽

Calcium Deposits ◽

Intense Stimulation

It Is known that Ca++ enters the muscle fiber at the junctional area during the action of the neurotransmitter, acetylcholine (ACh). Pappas and Rose demonstrated that following Intense stimulation, calcium deposits are found In the postsynaptic muscle membrane, Indicating the existence of calcium uptake In the postsynaptic area following ACh release. In addition to this calcium uptake, when mammal Ian skeletal muscles are exposed to a sustained action of the neurotransmitter, muscle damage develops. These same effects, l.e., Increased transmitter release, calcium uptake and finally muscle damage, can be obtained by Incubating the muscle with lonophore A23178.

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Fate of sperm membrane in fertilized ova

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100146047 ◽

1993 ◽

Vol 51 ◽

pp. 40-41

Author(s):

Frank J. Longo

Keyword(s):

Electron Microscopy ◽

Electrical Activity ◽

Sea Urchins ◽

Morphological Changes ◽

Integrated System ◽

Electrophysiological Recording ◽

Experimental Conditions ◽

The Status ◽

Sperm Membrane ◽

Temporal And Spatial

Measurement of the egg's electrical activity, the fertilization potential or the activation current (in voltage clamped eggs), provides a means of detecting the earliest perceivable response of the egg to the fertilizing sperm. By using the electrical physiological record as a “real time” indicator of the instant of electrical continuity between the gametes, eggs can be inseminated with sperm at lower, more physiological densities, thereby assuring that only one sperm interacts with the egg. Integrating techniques of intracellular electrophysiological recording, video-imaging, and electron microscopy, we are able to identify the fertilizing sperm precisely and correlate the status of gamete organelles with the first indication (fertilization potential/activation current) of the egg's response to the attached sperm. Hence, this integrated system provides improved temporal and spatial resolution of morphological changes at the site of gamete interaction, under a variety of experimental conditions. Using these integrated techniques, we have investigated when sperm-egg plasma membrane fusion occurs in sea urchins with respect to the onset of the egg's change in electrical activity.

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Fit Vs Faint: Assessing Work Causation in “Idiopathic Fall” Cases

Guides Newsletter ◽

10.1001/amaguidesnewsletters.2014.sepoct01 ◽

2014 ◽

Vol 19 (5) ◽

pp. 3-12

Author(s):

Lorne Direnfeld ◽

David B. Torrey ◽

Jim Black ◽

LuAnn Haley ◽

Christopher R. Brigham

Keyword(s):

Blood Flow ◽

Electrical Activity ◽

Loss Of Consciousness ◽

Brain Electrical Activity ◽

Medical Terminology ◽

Scientific Analysis ◽

Medical Causation ◽

The Individual ◽

The Brain ◽

Current Science

Abstract When an individual falls due to a nonwork-related episode of dizziness, hits their head and sustains injury, do workers’ compensation laws consider such injuries to be compensable? Bearing in mind that each state makes its own laws, the answer depends on what caused the loss of consciousness, and the second asks specifically what happened in the fall that caused the injury? The first question speaks to medical causation, which applies scientific analysis to determine the cause of the problem. The second question addresses legal causation: Under what factual circumstances are injuries of this type potentially covered under the law? Much nuance attends this analysis. The authors discuss idiopathic falls, which in this context means “unique to the individual” as opposed to “of unknown cause,” which is the familiar medical terminology. The article presents three detailed case studies that describe falls that had their genesis in episodes of loss of consciousness, followed by analyses by lawyer or judge authors who address the issue of compensability, including three scenarios from Arizona, California, and Pennsylvania. A medical (scientific) analysis must be thorough and must determine the facts regarding the fall and what occurred: Was the fall due to a fit (eg, a seizure with loss of consciousness attributable to anormal brain electrical activity) or a faint (eg, loss of consciousness attributable to a decrease in blood flow to the brain? The evaluator should be able to fully explain the basis for the conclusions, including references to current science.

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