Bioink Rheology Regulates Stability of Bioprinted Strands

2022 ◽  
Author(s):  
Dhanvanth J.S. Talluri ◽  
HuanTan Nguyen ◽  
Reza Avazmohammadi ◽  
Amir K. Miri
Keyword(s):  
Ad Hoc ◽  
Three Dimensional ◽  
Shear Thickening ◽  
Extrusion Process ◽  
Nozzle Geometry ◽  
Design Parameters ◽  
Enhanced Resolution ◽  
Key Factor ◽  
Thickening Behavior ◽  
Cellular Components

Abstract Extrusion three-dimensional (3D) bioprinting typically requires an ad-hoc trial-and-error optimization of the bioink composition towards enhanced resolution. The bioink solutions are solidified after leaving cone-shaped or cylindrical nozzles. The presence of bioink instability not only hampers the extrusion resolution but also affects the behavior of embedded cellular components. This is a key factor in selecting bioinks and bioprinting design parameters for well-established desktop and handheld bioprinters. In this work, we developed an analytical solution for the process of bioink deposition and compared its predictions against numerical simulations of the deposition. We estimated the onset of bioink instability as a function of bioink rheological properties and nozzle geometry. Both analytical and simulation results demonstrated that enhancing shear-thinning behavior of the bioink stabilizes the printing process whereas bioink shear-thickening behavior induces an opposite effect through extending the toe region of the deposition. The present study serves as a benchmark for detailed simulations of the extrusion process for optimal bioprinting.

scholarly journals Design, Optimization and Analysis of Supersonic Radial Turbines

2019 ◽  
Author(s):  
Lukas Benjamin Inhestern ◽  
James Braun ◽  
Guillermo Paniagua ◽  
José Ramón Serrano Cruz
Keyword(s):  
High Performance ◽  
Ad Hoc ◽  
Three Dimensional ◽  
Critical Factor ◽  
Design Tool ◽  
Navier Stokes ◽  
Design Parameters ◽  
Design Variables ◽  
Compact Design ◽  
Radial Outflow

Abstract New compact engine architectures such as pressure gain combustion require ad-hoc turbomachinery to ensure an adequate range of operation with high performance. A critical factor for supersonic turbines is to ensure the starting of the flow passages, which limits the flow turning and airfoil thickness. Radial outflow turbines inherently increase the cross section along the flow path, which holds great potential for high turning of supersonic flow with a low stage number and guarantees a compact design. First the preliminary design space is described. Afterwards a differential evolution multi-objective optimization with 12 geometrical design parameters is deducted. With the design tool AutoBlade 10.1, 768 geometries were generated and hub, shroud, and blade camber line were designed by means of Bezier curves. Outlet radius, passage height, and axial location of the outlet were design variables as well. Structured meshes with around 3.7 million cells per passage were generated. Steady three dimensional Reynolds averaged Navier Stokes (RANS) simulations, enclosed by the k-omega SST turbulence model were solved by the commercial solver CFD++. The geometry was optimized towards low entropy and high power output. To prove the functionality of the new turbine concept and optimization, a full wheel unsteady RANS simulation of the optimized geometry exposed to a nozzled rotating detonation combustor (RDC) has been performed and the advantageous flow patterns of the optimization were also observed during transient operation.

scholarly journals Design, Optimization, and Analysis of Supersonic Radial Turbines

2020 ◽  
Vol 142 (3) ◽  
Author(s):  
Lukas Benjamin Inhestern ◽  
James Braun ◽  
Guillermo Paniagua ◽  
José Ramón Serrano Cruz
Keyword(s):  
High Performance ◽  
Ad Hoc ◽  
Three Dimensional ◽  
Critical Factor ◽  
Design Tool ◽  
Navier Stokes ◽  
Design Parameters ◽  
Design Variables ◽  
Compact Design ◽  
Radial Outflow

Abstract New compact engine architectures such as pressure gain combustion require ad hoc turbomachinery to ensure an adequate range of operation with high performance. A critical factor for supersonic turbines is to ensure the starting of the flow passages, which limits the flow turning and airfoil thickness. Radial outflow turbines inherently increase the cross section along the flow path, which holds great potential for high turning of supersonic flow with a low stage number and guarantees a compact design. First, the preliminary design space is described. Afterward a differential evolution multi-objective optimization with 12 geometrical design parameters is deducted. With the design tool autoblade 10.1, 768 geometries were generated and hub, shroud, and blade camber line were designed by means of Bezier curves. Outlet radius, passage height, and axial location of the outlet were design variables as well. Structured meshes with around 3.7 × 106 cells per passage were generated. Steady three-dimensional (3D) Reynolds-averaged Navier–Stokes (RANS) simulations, enclosed by the k-omega shear stress transport turbulence model were solved by the commercial solver CFD++. The geometry was optimized toward low entropy and high-power output. To prove the functionality of the new turbine concept and optimization, a full wheel unsteady RANS simulation of the optimized geometry exposed to a nozzled rotating detonation combustor (RDC) has been performed and the advantageous flow patterns of the optimization were also observed during transient operation.

Freeze-Fracture Replication in the Ultrastructure of Blue-Green Algae

1967 ◽  
Vol 25 ◽  
pp. 74-75
Author(s):  
L. V. Leak
Keyword(s):  
Cell Wall ◽  
Electron Microscope ◽  
Green Algae ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Electron Microscopic ◽  
Photosynthetic Membranes ◽  
Blue Green Algae ◽  
Cell Biol ◽  
Cellular Components

Electron microscopic observations of freeze-fracture replicas of Anabaena cells obtained by the procedures described by Bullivant and Ames (J. Cell Biol., 1966) indicate that the frozen cells are fractured in many different planes. This fracturing or cleaving along various planes allows one to gain a three dimensional relation of the cellular components as a result of such a manipulation. When replicas that are obtained by the freeze-fracture method are observed in the electron microscope, cross fractures of the cell wall and membranes that comprise the photosynthetic lamellae are apparent as demonstrated in Figures 1 & 2.A large portion of the Anabaena cell is composed of undulating layers of cytoplasm that are bounded by unit membranes that comprise the photosynthetic membranes. The adjoining layers of cytoplasm are closely apposed to each other to form the photosynthetic lamellae. Occassionally the adjacent layers of cytoplasm are separated by an interspace that may vary in widths of up to several 100 mu to form intralamellar vesicles.

Mitochondrial Reconstructions Based on Serial Thick Sections and HVEM

1975 ◽  
Vol 33 ◽  
pp. 286-287
Author(s):  
Jerome J. Paulin
Keyword(s):  
Imaging Techniques ◽  
Three Dimensional ◽  
Posterior Pole ◽  
Dimensional Structure ◽  
Stereo Imaging ◽  
Thin Sections ◽  
Anatomical Features ◽  
The Past ◽  
Cellular Components ◽  
Mitochondrial Reticulum

Within the past decade it has become apparent that HVEM offers the biologist a means to explore the three-dimensional structure of cells and/or organelles. Stereo-imaging of thick sections (e.g. 0.25-10 μm) not only reveals anatomical features of cellular components, but also reduces errors of interpretation associated with overlap of structures seen in thick sections. Concomitant with stereo-imaging techniques conventional serial Sectioning methods developed with thin sections have been adopted to serial thick sections (≥ 0.25 μm). Three-dimensional reconstructions of the chondriome of several species of trypanosomatid flagellates have been made from tracings of mitochondrial profiles on cellulose acetate sheets. The sheets are flooded with acetone, gluing them together, and the model sawed from the composite and redrawn.The extensive mitochondrial reticulum can be seen in consecutive thick sections of (0.25 μm thick) Crithidia fasciculata (Figs. 1-2). Profiles of the mitochondrion are distinguishable from the anterior apex of the cell (small arrow, Fig. 1) to the posterior pole (small arrow, Fig. 2).

Methods for three-dimensional reconstruction of cellular components within a thick section

1986 ◽  
Vol 44 ◽  
pp. 18-21
Author(s):  
J. Frank ◽  
B. F. McEwen ◽  
M. Radermacher ◽  
C. L. Rieder
Keyword(s):  
Tilt Angle ◽  
Tomographic Reconstruction ◽  
3D Structure ◽  
Three Dimensional ◽  
Thick Section ◽  
Three Dimensional Reconstruction ◽  
Axis Ratio ◽  
Dimensional Reconstruction ◽  
Cellular Components

The tomographic reconstruction from multiple projections of cellular components, within a thick section, offers a way of visualizing and quantifying their three-dimensional (3D) structure. However, asymmetric objects require as many views from the widest tilt range as possible; otherwise the reconstruction may be uninterpretable. Even if not for geometric obstructions, the increasing pathway of electrons, as the tilt angle is increased, poses the ultimate upper limitation to the projection range. With the maximum tilt angle being fixed, the only way to improve the faithfulness of the reconstruction is by changing the mode of the tilting from single-axis to conical; a point within the object projected with a tilt angle of 60° and a full 360° azimuthal range is then reconstructed as a slightly elliptic (axis ratio 1.2 : 1) sphere.

scholarly journals Acoustic Cell Patterning in Hydrogel for Three-Dimensional Cell Network Formation

Micromachines ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 3
Author(s):  
Kyo-in Koo ◽  
Andreas Lenshof ◽  
Le Thi Huong ◽  
Thomas Laurell
Keyword(s):  
Network Formation ◽  
Umbilical Vein ◽  
Three Dimensional ◽  
Extrusion Process ◽  
Fibroblast Cells ◽  
Glass Capillary ◽  
Cell Network ◽  
Significant Difference ◽  
The One ◽  
Umbilical Vein Endothelial Cells

In the field of engineered organ and drug development, three-dimensional network-structured tissue has been a long-sought goal. This paper presents a direct hydrogel extrusion process exposed to an ultrasound standing wave that aligns fibroblast cells to form a network structure. The frequency-shifted (2 MHz to 4 MHz) ultrasound actuation of a 400-micrometer square-shaped glass capillary that was continuously perfused by fibroblast cells suspended in sodium alginate generated a hydrogel string, with the fibroblasts aligned in single or quadruple streams. In the transition from the one-cell stream to the four-cell streams, the aligned fibroblast cells were continuously interconnected in the form of a branch and a junction. The ultrasound-exposed fibroblast cells displayed over 95% viability up to day 10 in culture medium without any significant difference from the unexposed fibroblast cells. This acoustofluidic method will be further applied to create a vascularized network by replacing fibroblast cells with human umbilical vein endothelial cells.

Design and optimization of bump (compression surface) for diverterless supersonic inlet

2021 ◽  
pp. 095441002110029
Author(s):  
Irsalan Arif ◽  
Hassan Iftikhar ◽  
Ali Javed
Keyword(s):  
Fitness Function ◽  
Supersonic Jet ◽  
Three Dimensional ◽  
Flow Characteristics ◽  
Least Square ◽  
Pressure Recovery ◽  
Design Parameters ◽  
Inlet System ◽  
Design And Optimization ◽  
Supersonic Inlet

In this article design and optimization scheme of a three-dimensional bump surface for a supersonic aircraft is presented. A baseline bump and inlet duct with forward cowl lip is initially modeled in accordance with an existing bump configuration on a supersonic jet aircraft. Various design parameters for bump surface of diverterless supersonic inlet systems are identified, and design space is established using sensitivity analysis to identify the uncertainty associated with each design parameter by the one-factor-at-a-time approach. Subsequently, the designed configurations are selected by performing a three-level design of experiments using the Box–Behnken method and the numerical simulations. Surrogate modeling is carried out by the least square regression method to identify the fitness function, and optimization is performed using genetic algorithm based on pressure recovery as the objective function. The resultant optimized bump configuration demonstrates significant improvement in pressure recovery and flow characteristics as compared to baseline configuration at both supersonic and subsonic flow conditions and at design and off-design conditions. The proposed design and optimization methodology can be applied for optimizing the bump surface design of any diverterless supersonic inlet system for maximizing the intake performance.

scholarly journals Research on the Computed Tomography Pebble Flow Detecting System for HTR-PM

2017 ◽  
Vol 2017 ◽  
pp. 1-13
Author(s):  
Xin Wan ◽  
Ximing Liu ◽  
Jichen Miao ◽  
Peng Cong ◽  
Yuai Zhang ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Three Dimensional ◽  
Helical Ct ◽  
Design Parameters ◽  
Three Dimensional Model ◽  
Safe Operation ◽  
Cross Sectional ◽  
Ct System ◽  
Near Future ◽  
Pebble Flow

Pebble dynamics is important for the safe operation of pebble-bed high temperature gas-cooled reactors and is a complicated problem of great concern. To investigate it more authentically, a computed tomography pebble flow detecting (CT-PFD) system has been constructed, in which a three-dimensional model is simulated according to the ratio of 1 : 5 with the core of HTR-PM. A multislice helical CT is utilized to acquire the reconstructed cross-sectional images of simulated pebbles, among which special tracer pebbles are designed to indicate pebble flow. Tracer pebbles can be recognized from many other background pebbles because of their heavy kernels that can be resolved in CT images. The detecting principle and design parameters of the system were demonstrated by a verification experiment on an existing CT system in this paper. Algorithms to automatically locate the three-dimensional coordinates of tracer pebbles and to rebuild the trajectory of each tracer pebble were presented and verified. The proposed pebble-detecting and tracking technique described in this paper will be implemented in the near future.

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