scholarly journals Yolk sac–embryo distance in correlation with soluble form of vascular endothelial growth factor levels in pregnancy with potentially reserved evolutivity

2018 ◽  
Vol 20 (4) ◽  
pp. 487
Author(s):  
Carmen Elena Bucuri ◽  
Razvan Ciortea ◽  
Doru Diculescu ◽  
Andrei Malutan ◽  
Radu Mocan-Hognogi ◽  
...  
Keyword(s):  
Growth Factor ◽  
First Trimester ◽  
Yolk Sac ◽  
Soluble Form ◽  
Control Group ◽  
Case Group ◽  
Crown Rump Length ◽  
Low Level ◽  
First Trimester Pregnancy ◽  
Endothelial Growth Factor

Aims: Embryonic demise is a frequent complication of the first trimester pregnancy. The purpose of this study was to evaluate the correlation between a serum biomarker, the soluble form of the vasculo-endothelial growth factor (sFlt-1) and the distance between the yolk sac (YS) and embryo (DYSE), determined by ultrasonography.Material and methods: The study was a prospective case-control study that included 2 groups of patients – the control group with 81 first-trimester pregnancies in evolution and the case group with 89 first-trimester pregnancies with a potentially reserved evolutivity.Results: A correlation between the serum level of sFlt-1 and DYSE in embryos with crown-rump length (CRL) greater than 5 mm was identified, showing that a DYSE ≤3 mm correlates with a low level of sFlt-1 (p<0.05) and a DYSE> 4 mm correlates with an increased level of sFlt-1 (p<0.05).Conclusions: A low level of sFlt-1 associated with a distance between the embryo and yolk sac of small dimensions, respectively <3 mm, correlates with an increased rate of non-viable embryos. This correlation between an ultrasound and a serum parameter is of great value and brings important information about the viability of firsttrimester pregnancies.

scholarly journals Progesterone's Serum Level and a New Ultrasonographic Parameter in the First Trimester Pregnancy – Prognostic Factors for Embryonic Demise

2019 ◽  
Vol 41 (09) ◽  
pp. 525-530
Author(s):  
Carmen Elena Bucuri ◽  
Razvan Ciortea ◽  
Andrei Mihai Malutan ◽  
Costin Berceanu ◽  
Maria Patricia Rada ◽  
...  
Keyword(s):  
Serum Level ◽  
Chromosomal Abnormalities ◽  
First Trimester ◽  
Yolk Sac ◽  
Diagnostic Methods ◽  
Control Group ◽  
Case Group ◽  
Screening Methods ◽  
Control Analysis ◽  
Serum Progesterone

Abstract Objective The etiology of embryonic demise is multifactorial, with chromosomal abnormalities being the most common (40%). The purpose of the present study is to evaluate the correlation between a serum biomarker, progesterone, and an ultrasonographic parameter, the distance between yolk sac and embryo (DYSE) in assessing the prognosis of pregnancy outcome in the 1st trimester. Methods The present study is a prospective case-control analysis that includes 2 groups of patients: 81 patients with first-trimester normal evolutive pregnancy and 89 patients with embryonic demise, all of the patients having between 6 and 11 weeks of amenorrhea. Endovaginal ultrasonographic exploration was performed to evaluate the distance between the lower pole of the embryo and the yolk sac. From each subject enrolled in the study, 20 ml of blood was collected for progesterone serum level measurement. Results Regarding the DYSE in the case group, lower values were observed compared with the control group, the difference being statistically significant. In the statistical analysis of serum progesterone values, statistically significant differences were observed between the 2 groups (p < 0.05). Conclusion The DYSE has a high positive predictive value in identifying pregnancies with potentially reserved outcome, with the present study demonstrating that a DYSE < 3 mm causes an unfavorable evolution of the pregnancy. Low serum levels of progesterone are associated with an increased rate of nonviable embryos. The correlation between these two parameters increases the effectiveness of screening methods in prenatal monitoring and improves the diagnostic methods for the first-trimester pregnancies whose outcome potential can be reserved.

scholarly journals Investigating the Relationship between Serum Level of s-Met (Soluble Hepatic Growth Factor Receptor) and Preeclampsia in the First and Second Trimesters of Pregnancy

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Farshad Naghshvar ◽  
Zhila Torabizadeh ◽  
Narges Moslemi Zadeh ◽  
Hooman Mirbaha ◽  
Parand Gheshlaghi
Keyword(s):  
Growth Factor ◽  
Serum Level ◽  
Growth Factor Receptor ◽  
Serum Levels ◽  
Severe Preeclampsia ◽  
Soluble Form ◽  
Control Group ◽  
Case Group ◽  
Mortality And Morbidity ◽  
Hepatic Growth Factor

Introduction. Preeclampsia (PE) is an important cause of mortality and morbidity for mothers, fetuses, and the newborns. Placenta plays a pivotal role in pathogenesis of PE. Hepatic growth factor (HGF) is a cytokine expressed by the mesenchymal stalk of placental villi during pregnancy and assumes a paracrine role in trophoblasts which express its receptor (c-MET). In the present study, we investigate the diagnostic value of s-Met (the soluble form of the receptor) in the first and second trimesters of pregnancy for early diagnosis of preeclampsia. Method and Materials. This is a case-control study conducted on 95 pregnant women. The serum level of s-Met was measured in the first and second trimesters, and the participants were followed until delivery. 44 individuals with preeclampsia (the case group) and 51 individuals without preeclampsia (the control group) were evaluated. Results. Serum level of s-Met in preeclamptic participants was lower than that of the control group in both the first and the second trimesters (P<0.0001). In addition, serum levels of s-Met were significantly lower during the first and second trimesters in patients with early, severe preeclampsia compared to those with late, mild preeclampsia (P<0.0001). The sensitivity and specificity of s-Met in the first and second trimesters were, respectively, (83%, 94%) and (77%, 94%) for early preeclampsia and (88%, 92%) and (86%, 98%) for severe preeclampsia. Conclusion. Considering our findings, serum level of s-Met may be used as a predictive factor for early detection of preeclampsia. Further research is required to corroborate the functional and therapeutic value of s-Met in preeclampsia.

scholarly journals Expression levels of circulatory mir-185-5p, vascular endothelial growth factor, and platelet-derived growth factor target genes in endometriosis

2020 ◽  
Author(s):  
Mohammad Hossein Razi ◽  
Maryam Eftekhar ◽  
Nasrin Ghasemi ◽  
Mohammad Hasan Sheikhha ◽  
Ali Dehghani Firoozabadi
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Target Genes ◽  
Platelet Derived Growth Factor ◽  
Control Group ◽  
Case Group ◽  
Vascular Endothelial ◽  
Blood Samples ◽  
Qrt Pcr ◽  
Endothelial Growth Factor

Background: Using blood-based biomarkers such as microRNAs (miRNAs) may allow particularly effective and minimally invasive diagnosis and treatment of endometriosis. Objective: We evaluated the differential expression of circulating miRNA-185-5p (miR- 185-5p), vascular endothelial growth factor (VEGF), and platelet-derived growth factor (PDGF) target genes between endometriosis and healthy women. Materials and Methods: 25 women with a history of endometriosis (grad III-IV) diagnosed by laparoscopy as the case group and 25 women without endometriosis underwent laparoscopy for ovarian cysts or pelvic pain as the control group were enrolled in this case-control study. Blood samples were obtained, and total RNA was used for high-throughput small RNA sequencing, and this was confirmed by means of quantitative real-time polymerase chain reaction (qRT-PCR). Results: miRNA expression profiling using non-coding RNA sequencing revealed that one miRNA including miR-185-5p was significantly down-regulated in the case group compared with the controls. The qRT-PCR results showed significant downregulation of the expression level of miR-185-5p (p < 0.01) in the plasma of the case group. Receiver operating characteristic (ROC) curve analysis showed the area of miR-185-5p under the ROC curve for endometriosis diagnosis was 0.919 (p < 0.001). The RT-PCR results demonstrated that there was no significant difference in the expression of VEGF and PDGF mRNA of blood samples in the cases compared to the control group (PDGF, p = 0.09 and VEGF, p = 0.36). Conclusion: The low expression of miR-185-5p in the plasma of women with endometriosis could be employed as an important non-invasive biomarker for early detection and screening of endometriosis by blood samples. Key words: Biomarker, miRNA, Diagnosis, Endometriosis, Angiogenesis.

scholarly journals Impact of High-normal Serum TSH with First Trimester Pregnancy Loss: A Case-control Study in Tertiary Care Hospitals in Bangladesh

2018 ◽  
Vol 1 (1) ◽  
pp. 29-35
Author(s):  
Yisrat Jahan ◽  
M Anwar Hussain ◽  
Rezaul Karim Kazal ◽  
Mohammad Akhteruzzaman ◽  
Rifat Jahan
Keyword(s):  
Pregnancy Loss ◽  
Tertiary Care ◽  
First Trimester ◽  
Normal Serum ◽  
Control Group ◽  
Case Group ◽  
First Trimester Pregnancy ◽  
Trimester Pregnancy ◽  
Tertiary Care Hospitals ◽  
Serum Tsh

The relationship between existing thyroid autoimmunity and the probability of spontaneous abortion has been the subject of a number of studies. In this study, we evaluate the impact of `high normal' serum TSH levels (2.5-5.0 mlU/L) on first trimester pregnancy loss in anti-TPO Ab negative women in Bangladesh. Specifically, we wanted to know the average serum TSH levels for women who had a miscarriage (case group) and who did not have a miscarriage (control group) during the first trimester of pregnancy. Unmatched case-control study involving 119 women in their first trimester was conducted during July 2011 to June 2012 in three tertiary care hospitals in Bangladesh. We found average TSH level for the control group (1.42$\pm$0.86 mlU/L) to be lower than the average for the case group (1.74$\pm$1.15 mlU/L). However, we did not find statistically significant association between the serum TSH level and first-trimester pregnancy loss. The findings of this study will provide a baseline for future studies on this topic involving Bangladeshi women. In addition, our study adds to the growing discussion on redefining the lower limit for the `high normal' serum TSH level among the anti-TPO Ab negative women.

scholarly journals Association of TSH level above 2.1 mlU/L and first trimester pregnancy loss in anti-TPO antibody negative women

2015 ◽  
Author(s):  
Yisrat Jahan ◽  
Enayetur Raheem ◽  
Mohammad Akteruzzaman ◽  
M Anwar Hussain ◽  
Rezaul Karim ◽  
...  
Keyword(s):  
Pregnancy Loss ◽  
Local Level ◽  
First Trimester ◽  
Control Group ◽  
Case Group ◽  
Thyroid Autoantibody ◽  
First Trimester Pregnancy ◽  
Trimester Pregnancy ◽  
Group I ◽  
First Trimester Of Pregnancy

Although the exact level of TSH that is indicative of risk of pregnancy loss is not known, a number of studies have suggested a range of values for TSH level that are associated with first trimester pregnancy loss. We conducted an unmatched case-control study to test if a TSH level above 2.1 mlU/L is associated with first trimester pregnancy loss in anti-TPO antibody negative women. We found relatively higher number of women in the case group (18) whose TSH level was above 2.1 mlU/L compared to 7 women in control group. When considered patients in Group I (TSH ≤2.1 mlU/L), 45.74% had miscarriage while 54.26% did not have miscarriage within first trimester of pregnancy. Among the Group II patients (TSH >2.1 mlU/L), 78% had miscarriage and 28% did not have miscarriage. Noticeably there is a larger proportion of miscarriage among the women with TSH level above 2.1 mlU/L. The association between TSH level and first trimester pregnancy loss was statistically significant (p=.0196). From the multivariate analysis, odds ratio for TSH level (OR 4.0, 95% CI: 1.44-11.16) indicates that odds of having miscarriage whose TSH level is above 2.1 mlU/L is 4 times compared to those with TSH level below 2.1 mlU/L after adjusting for the effects of age and BMI. At a global level, the findings of this study provide evidence to the existing discussion on redefining the upper limit of TSH level that is related to first trimester pregnancy loss. At the local level, the results will have direct implication in facilitating management of future pregnancies particularly during the first trimester among Bangladeshi thyroid autoantibody negative women.

scholarly journals Effects of miRNA-200b on the development of diabetic retinopathy by targeting VEGFA gene

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
En-Hui Li ◽  
Qin-Zhu Huang ◽  
Gao-Chun Li ◽  
Zhen-Yang Xiang ◽  
Xin Zhang
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Target Gene ◽  
Control Group ◽  
Case Group ◽  
Vascular Endothelial ◽  
Qrt Pcr ◽  
Endothelial Growth Factor ◽  
Vegfa Gene ◽  
Tgf Β1

The present study explored the effect of miR-200b on the development of diabetic retinopathy (DR) by targeting vascular endothelial growth factor A (VEGFA) gene. The study populations consisted of 255 DR patients (case group) and 253 healthy people (control group), while the expressions of miR-200b and VEGFA mRNA were detected by quantitative real-time PCR (qRT-PCR). Bioinformatics software and dual-luciferase reporter assay were used to confirm VEGFA as a target gene of miR-200b. Also, a total of 70 Wistar male rats were selected and randomly assigned into blank, normal control (NC), miR-200b mimics, miR-200b inhibitors, miR-200b inhibitors + silencing vascular endothelial growth factor A (siVEGFA), and siVEGFA groups (n=10/group) respectively. Streptozotocin (STZ)-induced rat models of DR were successfully established. VEGFA, transforming growth factor-β1 (TGF-β1), hepatocyte growth factor (HGF), and pigment epithelium-derived factor (PEDF) were detected using qRT-PCR and Western blotting. In comparison with the control group, the case group showed lower expression of miR-200b but higher expression of VEGFA mRNA. VEGFA was confirmed as a target gene of miR-200b. Rats in the miR-200b mimics and siVEGFA groups exhibited higher expression of PEDF mRNA and protein but lower expressions of VEGFA, TGF-β1, HGF protein, and mRNA than the NC group. There was no remarkable difference in expressions of PEDF, VEGFA, TGF-β1, HGF protein, and mRNA between the miR-200b inhibitors + siVEGFA and NC groups. In conclusion, the present study demonstrated that miR-200b might alleviate DR development by down-regulating its target gene VEGFA.

Effect of HylocereusPolyrhizus Rind Extract Toward Interleukin-1β, Vascular Endothelial Growth Factor Expression, Endometriosis Implant Area

2017 ◽  
Vol 9 (08) ◽  
Author(s):  
YanuarEka P. ◽  
Hendy Hendarto ◽  
Widjiati .
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Treatment Group ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Vascular Endothelial ◽  
Mice Model ◽  
Endothelial Growth Factor ◽  
Fruit Rind

Retrograde menstruation lead to I Kappa B Kinase (IKK) fosforilation in peritoneum macrophage and cause secretion of proinflammatory cytokine interleukin1β then stimulate endometriosis cell to produce Vascular Endothelial Growth Factor which lead to increasing of endometriosis lession seen as endometriosis implant area. Cytokine secretion was inhibited through prevention of NF-κB activation by dragon red fruit rind extract (Hylocereuspolyrhizus). The aim of this reserach is to know the effect of dragon red fuit rind extract with 0,25; 0,5; and 1 mg/g bodyweight dosage toward IL-1β, VEGF expression and implant area in endometriosis mice model. The design of this experiment was randomized post test only control group design.Endometrios mice model were made in 14 days and split into two group, positive control group and treatment group after two week negative control group and postive control group were given Na-CMC 0,5% solution consequetively, and treatment group were given dragon red fruit extract with different dosage. Signification number for IL-1β is p>0,05, signification number for VEGF is p>0,05, and implant area signification number is p>0,05. Administration of dragon red fruit rind extract can decrease IL-1β, VEGF, and implant area.

The distance between the embryo and yolk sac associated with inhibin A in the first-trimester pregnancy – which is the novelty?

2019 ◽  
Vol 1 (67) ◽  
pp. 13
Author(s):  
Carmen Elena Bucuri ◽  
Răzvan  Ciortea ◽  
Andrei Mihai Malutan ◽  
Cristian Iuhaș ◽  
Maria Rada ◽  
...  
Keyword(s):  
First Trimester ◽  
Yolk Sac ◽  
Inhibin A ◽  
First Trimester Pregnancy ◽  
Trimester Pregnancy

scholarly journals Vascular endothelial growth factor in prognosis of splenic malignant tumours in dogs

2014 ◽  
Vol 58 (2) ◽  
pp. 255-260
Author(s):  
Aleksandra Sobczyńska-Rak ◽  
Izabela Polkowska ◽  
Adam Brodzki
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Comparative Analysis ◽  
Growth Factor ◽  
Who Classification ◽  
Control Group ◽  
Vascular Endothelial ◽  
Malignant Tumours ◽  
Blood Samples ◽  
Endothelial Growth Factor ◽  
Immunoenzymatic Assays

Abstract The aim of the study was to determine the levels of the vascular endothelial growth factor (VEGF) in the serum of dogs suffering from splenic malignant tumours, prior to splenectomy, as well as three and six months after the surgery. Tumours and blood samples were collected from 10 dogs of various breeds, aged between 7 and 13 years, and from 10 control animals. Tumour sections were fixed in 10% buffered formalin for 24 h. The type of tumour was determined according to the WHO classification. Blood samples were centrifuged and the obtained sera were subjected to immunoenzymatic assays to determine the VEGF levels. The median of VEGF levels in the serum of dogs suffering from splenic malignant tumours was 37.85 pg/mL (15.40-107.18 pg/mL). The highest values were observed in dogs with confirmed metastases (107.18 pg/mL and 65.43 pg/mL). The VEGF values in control group were between 0.1 pg/mL and 13.04 pg/mL. A comparative analysis of the VEGF levels against the animals' survival time indicated that VEGF overexpression may serve as a prognostic factor in cases of malignant tumours of the spleen.

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