Effect of sample storage conditions on Er:YAG laser ablation of enamel, dentin, and bone

2007 ◽  
Author(s):  
Wayne J. Selting
Keyword(s):  
Laser Ablation ◽  
Er:Yag Laser ◽  
Storage Conditions ◽  
Sample Storage

Effect of Sample Storage Conditions on Body Burden Analysis of Organic Contaminants in Unionid Mussels

1992 ◽  
Vol 27 (4) ◽  
pp. 833-844 ◽  
Author(s):  
Micheline Hanna
Keyword(s):  
Organic Contaminants ◽  
Body Burden ◽  
Storage Conditions ◽  
The Body ◽  
Sample Storage ◽  
Pcb Congeners ◽  
Unionid Mussels ◽  
Group A ◽  
The Difference ◽  
Group B

Abstract In order to quantitatively assess the effect of sample storage conditions on the body burden analysis of organic contaminants, a comparative analysis was carried out on the unionid mussel Elliptic complanata. The mussels were divided into two groups, each with distinct storage conditions, while Group A was kept in the freezer at −20°C, Group B was kept in the refrigerator for five days at 5°C. All the compounds present in the control were also present in Group B samples. Analysis of the organic contaminants in each of these two groups showed that for total PCB concentrations, the two treatments were not significantly different; however when compared individually 6 of the 13 PCB congeners showed significant differences. The observed differences were relatively small for individual PCB congeners (7.1 to 15.3%), higher for chlorobenzenes (10.5 to 36.4%), and yet higher for HCE (44.1%); the difference for HCE, although large is nevertheless not significant, even if only marginally so.

scholarly journals The effect of storage conditions on samples for the evaluation of copper status in blesbok (Damaliscus pygargus phillipsi)

2002 ◽  
Vol 73 (3) ◽  
Author(s):  
M. Quan ◽  
M.S. Mulders ◽  
D.G.A. Meltzer
Keyword(s):  
Superoxide Dismutase ◽  
Liquid Nitrogen ◽  
Liver Tissue ◽  
Room Temperature ◽  
Storage Conditions ◽  
Superoxide Dismutase Activity ◽  
Activity Levels ◽  
Sample Storage ◽  
Live Animal ◽  
Jersey Cows

Investigaltions to determine the effect of sample storage on the concentration of copper in liver tissue and on the activity of erythrocyte superoxide dismutase were undertaken in preparation for a study of blesbok (Damaliscus pygargus phillipsi) that were suspected to be suffering from copper deficiency. Two liver samples were collected from each of 20 culled blesbok in a manner that simulated the collection of biopsies from the live animal. These samples were stored either in 10 % formalin or frozen at -20 °C until analysed 4 1/2 months later. The effect of different methods of sample storage on superoxide dismutase activity was determined. Erythrocytes collected from 3 Jersey cows and 5 culled blesbok were washed and divided into 0.5m portions, stored at room temperature (~20 °C), in a refrigerator (4 °C), frozen at -20 °C in a freezer, and in liquid nitrogen (-200 °C). An analysis of superoxide dismutase activity was undertaken using a commercial assay kit at intervals of 2-4 days until the levels of activity had fallen significantly. The copper concentration in formalin-preserved liver samples was significantly lower than that measured in frozen liver tissue apparently as a result of leaching. The activity of superoxide dismutase in cattle blood was unchanged for 4 days at room temperature but fell appreciably after 2 days at 4 °C and -20 °C. Enzyme activity remained unchanged for 200 days in erythrocytes stored in liquid nitrogen. Superoxide dismutase activity levels in healthy blesbok were considerably lower than those measured in Jersey cows and remained unaffected for up to 6 days in samples stored at 4 °C and 20 °C. The level of activity fell significantly thereafter. Samples stored in liquid nitrogen were unchanged after 40 days.

Thermal alteration and morphological changes of sound and demineralized primary dentin after Er:YAG laser ablation

2011 ◽  
Vol 75 (2) ◽  
pp. 126-132 ◽  
Author(s):  
Cristina Bueno BrandÃO ◽  
Marta Maria Martins Giamatei Contente ◽  
FabrÍCio Augusto De Lima ◽  
Rodrigo Galo ◽  
Alessandra Marques CorrÊA-Afonso ◽  
...  
Keyword(s):  
Laser Ablation ◽  
Er:Yag Laser ◽  
Morphological Changes ◽  
Thermal Alteration

Preservation and Storage Mechanisms for Raw Milk Samples for Use in Milk-Recording Schemes

1996 ◽  
Vol 59 (2) ◽  
pp. 151-154 ◽  
Author(s):  
HUMBERTO G. MONARDES ◽  
ROBERT K. MOORE ◽  
BRIAN CORRIGAN ◽  
YVON RIOUX
Keyword(s):  
Cell Count ◽  
Somatic Cell Count ◽  
Potassium Dichromate ◽  
Dairy Herd ◽  
Raw Milk ◽  
Storage Conditions ◽  
Sample Storage ◽  
Milk Samples ◽  
Whole Process ◽  
And Storage

This study, carried out by the Quebec Dairy Herd Analysis Service, compares (during summer conditions in Quebec) the performance of three types of preservatives for raw milk under four different systems of sample storage: no refrigeration, refrigeration at the laboratory only, refrigeration during transport and at the lab, and complete refrigeration from sampling at the farm to analysis. The objective was to determine the best preservative and storage conditions for protecting milk components during transportation and storage of raw milk samples collected at the farm and sent to a central testing lab for analysis. Milk samples were analyzed at day 3 and at day 7 after sampling to observe the effect of aging. A total of 12,480 samples were collected during the trial. The components studied were percentage of fat and protein and somatic cell count (SCC). In general, samples preserved with bronopol (2-bromo-2-nitropropane-1,3-diol and 2-bromo-2-nitropropanol) in liquid or in microtab tended to give higher readings for fat and protein contents than samples preserved with potassium dichromate. Significantly lower fat values were observed in 7-day-old samples compared to 3-day-old samples. Fat depression was more accentuated in nonrefrigerated samples. Under current methods of handling raw milk samples, refrigeration during the whole process of sampling, transportation, and until analysis, seems an ideal to attain to avoid significant reductions of fat values.

scholarly journals Comparison of Four Commercial Kits for Isolation of Urinary Cell-Free DNA and Sample Storage Conditions

Diagnostics ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 234 ◽  
Author(s):  
Eun Young Lee ◽  
Eun-Ju Lee ◽  
Hana Yoon ◽  
Dong Hyeon Lee ◽  
Kwang Hyun Kim
Keyword(s):  
Cost Efficiency ◽  
High Sensitivity ◽  
Storage Conditions ◽  
Sample Storage ◽  
Sample Collection ◽  
Cell Free Dna ◽  
Free Dna ◽  
Zymo Research ◽  
Free Circulating Dna ◽  
Commercial Kits

Urinary cell-free DNA (cfDNA) is an attractive body fluid for liquid biopsy. In this study, we compared the efficiencies of four commercial kits for urinary cell-free DNA (cfDNA) isolation and of various sample storage conditions. Urinary cfDNA was isolated from 10 healthy individuals using four commercial kits: QIAamp Circulating Nucleic Acid Kit (QC; Qiagen), MagMAX™ Cell-Free DNA Isolation Kit (MM; Applied Biosystems), Urine Cell-Free Circulating DNA Purification Midi Kit (NU; Norgen Biotek), and Quick-DNA™ Urine Kit (ZQ; Zymo Research). To assess the isolation efficiency, an Agilent 2100 Bioanalyzer with High Sensitivity DNA chips was used, and cfDNA yield was defined as the amount of cfDNA obtained from 1 mL of urine. MM and QC provided the highest cfDNA yield in the 50–300 bp range, and MM and NU gave the highest cfDNA yield in the 50–100 bp range. In particular, the NU kit was efficient for isolation of more fragmented cfDNA in the range of 50–100 bp with the lowest cellular genomic DNA contamination. ZQ had the best cost-efficiency for isolating the same amount of urinary cfDNA. Samples stored at −70 °C with the addition of 10 mM EDTA resulted in the highest cfDNA yield 3 months after sample collection.

scholarly journals The Influence of Serum Sample Storage Conditions on Selected Laboratory Parameters Related to Oxidative Stress: A Preliminary Study

Diagnostics ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 51
Author(s):  
Lilla Pawlik-Sobecka ◽  
Katarzyna Sołkiewicz ◽  
Izabela Kokot ◽  
Aleksandra Kiraga ◽  
Sylwia Płaczkowska ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Serum Sample ◽  
Storage Conditions ◽  
Wilcoxon Test ◽  
Reference Ranges ◽  
Sample Storage ◽  
Serum Samples ◽  
The Stability ◽  
The Individual ◽  
The Impact

The present work aims at accessing the stability of biological material stored for diagnostic and scientific purposes. The influence of the temperature, storage time, and cyclic thawing on concentration stability of selected oxidative stress parameters in human serum was investigated. The study group consisted of 20 serum samples collected from healthy volunteers aged 18–52. The parameters whose reference ranges were not determined and to which validated determination methods did not correspond were examined by manual methods (FRAP and AOPP). Automatic methods were used to determine routine laboratory tests (albumin, total protein, bilirubin, uric acid) using the Konelab 20i® analyzer. The samples were stored at various temperatures (room temperature, 4 °C, −20 °C, −80 °C) for max 6 months and were subjected to cyclic thawing at 1 month intervals. In order to check whether any differences between the concentrations of the studied parameters existed when the samples were stored in various conditions, the paired Student t-test or Wilcoxon test and comparison to desirable bias were applied. Based on the obtained results, it was found that the temperature and time of serum sample storage significantly affected the stability of the analyzed parameters and determined different shelf lives of serum samples for oxidative stress examination. Therefore, continuing the investigation concerning the impact of storage conditions on various serum parameters seems justified due to the discrepancy between the individual results obtained by different researchers and the inconsistencies between the results of scientific research and the applicable recommendations.

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