Distribution of two major organ of Corti proteins in different component cell types

AbstractAn increasing volume of data suggests that changes in cellular metabolism have a major impact on the health of tissues and organs, including in the auditory system where metabolic alterations are implicated in both age-related and noise-induced hearing loss. However, the difficulty of access and the complex cyto-architecture of the organ of Corti has made interrogating the individual metabolic states of the diverse cell types present a major challenge. Multiphoton fluorescence lifetime imaging microscopy (FLIM) allows label-free measurements of the biochemical status of the intrinsically fluorescent metabolic cofactors NADH and NADPH with subcellular spatial resolution. However, the interpretation of NAD(P)H FLIM measurements in terms of the metabolic state of the sample are not completely understood. We have used this technique to explore changes in metabolism associated with hearing onset and with acquired (age-related and noise-induced) hearing loss. We show that these conditions are associated with altered NAD(P)H fluorescence lifetimes, use a simple cell model to confirm an inverse relationship between τbound and oxidative stress, and propose such changes as a potential index of oxidative stress applicable to all mammalian cell types.

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To the bones: mapping the skeletal LEPR + pool to component cell types

The EMBO Journal ◽

10.15252/embj.2021110343 ◽

2022 ◽

Author(s):

Jun Sun ◽

Matthew B Greenblatt

Keyword(s):

Cell Types ◽

Component Cell

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The morphological and functional development of the stria vascularis in miniature pigs

Reproduction Fertility and Development ◽

10.1071/rd15183 ◽

2017 ◽

Vol 29 (3) ◽

pp. 585 ◽

Cited By ~ 5

Author(s):

Weiwei Guo ◽

Haijin Yi ◽

Zhang Yan ◽

Lili Ren ◽

Lei Chen ◽

...

Keyword(s):

Animal Studies ◽

Stria Vascularis ◽

Organ Of Corti ◽

Lateral Wall ◽

Cell Types ◽

Endocochlear Potential ◽

Miniature Pigs ◽

Functional Development ◽

Transmission Electron ◽

Different Types

The purpose of this study was to examine the morphological and functional development of the lateral wall of the scala media of the cochlea in miniature pigs; light and transmission electron microscopy and electrophysiology were used for this purpose. We showed that the lateral wall of the scala media of the cochlea appears at embryonic Day 21 (E21) when the cochlear duct begins to form. From E28 to E49, the lateral wall can be distinguished according to its position along the cochlea. At E56, cells in the lateral wall begin to differentiate into three different types. At E70, three cell types, marginal, intermediate and basal, can be clearly distinguished. At E91, the stria vascularis is adult-like and the organ of Corti is also morphologically mature. The average endocochlear potential measured from the second turn of the cochlea (at E98, postnatal Day 1 (P1), P13 and P30) was 71.4 ± 2.5 (n = 7), 78.8 ± 1.5 (n = 10), 77.3 ± 2.3 (n = 10) and 78.0 ± 2.1 mV (n = 10), respectively. Our results suggest that in miniature pigs the stria vascularis develops during the embryonic period, concurrent with maturation of the organ of Corti. The magnitude of the endocochlear potential reached its mature level when the stria vascularis was morphologically adult-like at E98. These findings provide a morphological and functional basis for future animal studies using the miniature pig model concerning the pathogenesis of various inner-ear diseases.

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F-actin, tubulin and spectrin in the organ of Corti: Comparative distribution in different cell types and mammalian species

Hearing Research ◽

10.1016/0378-5955(94)90098-1 ◽

1994 ◽

Vol 76 (1-2) ◽

pp. 173-187 ◽

Cited By ~ 46

Author(s):

Yehoash Raphael ◽

Brian D. Athey ◽

Wang Yu ◽

Michael K. Lee ◽

Richard A. Altschuler

Keyword(s):

Mammalian Species ◽

Organ Of Corti ◽

Cell Types ◽

Different Cell Types

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Progress in electron microscopic diagnostics: semi-quantitative determination of precipitable calcium in different cell types of the organ of Corti in the guinea-pig

Journal of Microscopy ◽

10.1111/j.1365-2818.1991.tb03123.x ◽

1991 ◽

Vol 162 (1) ◽

pp. 133-140 ◽

Cited By ~ 3

Author(s):

U.-R. Heinrich ◽

J. Maurer ◽

W. Mann ◽

W. Kreutz

Keyword(s):

Guinea Pig ◽

Quantitative Determination ◽

Organ Of Corti ◽

Cell Types ◽

Electron Microscopic ◽

Different Cell Types

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The effect of tissue composition on gene co-expression

Briefings in Bioinformatics ◽

10.1093/bib/bbz135 ◽

2019 ◽

Cited By ~ 6

Author(s):

Yun Zhang ◽

Jonavelle Cuerdo ◽

Marc K Halushka ◽

Matthew N McCall

Keyword(s):

Expression Patterns ◽

Real Data ◽

Cell Types ◽

Tissue Level ◽

Tissue Composition ◽

Cell Type ◽

Tissue Samples ◽

Cell Type Composition ◽

Type Composition ◽

Component Cell

Abstract Variable cellular composition of tissue samples represents a significant challenge for the interpretation of genomic profiling studies. Substantial effort has been devoted to modeling and adjusting for compositional differences when estimating differential expression between sample types. However, relatively little attention has been given to the effect of tissue composition on co-expression estimates. In this study, we illustrate the effect of variable cell-type composition on correlation-based network estimation and provide a mathematical decomposition of the tissue-level correlation. We show that a class of deconvolution methods developed to separate tumor and stromal signatures can be applied to two component cell-type mixtures. In simulated and real data, we identify conditions in which a deconvolution approach would be beneficial. Our results suggest that uncorrelated cell-type-specific markers are ideally suited to deconvolute both the expression and co-expression patterns of an individual cell type. We provide a Shiny application for users to interactively explore the effect of cell-type composition on correlation-based co-expression estimation for any cell types of interest.

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Functional units in rainbow trout (Salmo gairdneri, Richardson) liver: III. Morphometric analysis of parenchyma, stroma, and component cell types

American Journal of Anatomy ◽

10.1002/aja.1001850107 ◽

1989 ◽

Vol 185 (1) ◽

pp. 58-73 ◽

Cited By ~ 67

Author(s):

James A. Hampton ◽

R. Clark Lantz ◽

David E. Hinton

Keyword(s):

Rainbow Trout ◽

Morphometric Analysis ◽

Cell Types ◽

Salmo Gairdneri ◽

Component Cell ◽

Functional Units

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TRIOBP promotes bidirectional radial stiffness gradients within the organ of Corti

10.1101/2021.06.28.450090 ◽

2021 ◽

Author(s):

Hesam Babahosseini ◽

Inna Belyantseva ◽

Rizwan Yousaf ◽

Risa Tona ◽

Shadan Hadi ◽

...

Keyword(s):

Organ Of Corti ◽

Cell Types ◽

Supporting Cell ◽

Sensory Epithelium ◽

Apical Surface ◽

Wild Type ◽

Radial Stiffness ◽

Genes Encoding ◽

Individual Contributions ◽

The Individual

Hearing depends on complex mechanical properties of the inner ear sensory epithelium. Yet, the individual contributions of different cell types to the stiffness spectrum of the sensory epithelium have not been thoroughly investigated. Using sub-100 nanometer spatial resolution PeakForce Tapping Atomic Force Microscopy (PFT-AFM), we mapped the Youngs modulus (stiffness) of the apical surface of different cells of freshly-dissected cochlear epithelium from wild-type mice and mice lacking the F-actin bundling protein TRIOBP-5 or TRIOBP-4 and TRIOBP-5. Variants of the genes encoding human and mouse TRIOBP are associated with deafness. We show that TRIOBP deficiency affects formation of supporting cell apical phalangeal microfilaments and bundled cortical F-actin of hair cell cuticular plates, softening the apical surface of the sensory epithelium. Unexpectedly, high-resolution PFT-AFM-mapping also revealed previously unrecognized reticular lamina radial stiffness gradients of opposite orientations in wild-type supporting and hair cells. Deafness-associated TRIOBP deficiencies significantly modified these bidirectional radial stiffness gradients.

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Whole Animal Multiplexed Single-Cell RNA-Seq Reveals Plasticity of Clytia Medusa Cell Types

10.1101/2021.01.22.427844 ◽

2021 ◽

Author(s):

Tara Chari ◽

Brandon Weissbourd ◽

Jase Gehring ◽

Anna Ferraioli ◽

Lucas Leclère ◽

...

Keyword(s):

High Resolution ◽

Single Cell ◽

Cell Types ◽

Model Organisms ◽

Traditional Model ◽

Rna Seq ◽

Batch Effects ◽

Future Studies ◽

Component Cell ◽

Jellyfish Species

AbstractWe present an organism-wide, transcriptomic cell atlas of the hydrozoan medusa Clytia hemisphaerica, and determine how its component cell types respond to starvation. Utilizing multiplexed scRNA-seq, in which individual animals were indexed and pooled from control and perturbation conditions into a single sequencing run, we avoid artifacts from batch effects and are able to discern shifts in cell state in response to organismal perturbations. This work serves as a foundation for future studies of development, function, and plasticity in a genetically tractable jellyfish species. Moreover, we introduce a powerful workflow for high-resolution, whole animal, multiplexed single-cell genomics (WHAM-seq) that is readily adaptable to other traditional or non-traditional model organisms.

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