Vitamin C Supplementation Affects Oxidative-Stress Blood Markers in Response to a 30-Minute Run at 75% VO2max

2005 ◽  
Vol 15 (3) ◽  
pp. 279-290 ◽  
Author(s):  
Allan H. Goldfarb ◽  
Stephen W. Patrick ◽  
Scott Bryer ◽  
Tongjian You
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Repeated Measures ◽  
Thiobarbituric Acid ◽  
Protein Carbonyls ◽  
Dependent Manner ◽  
Total Blood ◽  
Intensity Measures ◽  
Vitamin C Supplementation ◽  
Dose Dependent

Vitamin C supplementation (VC) (either 500 or 1000 mg/d for 2 wk) was compared to a placebo treatment (P) to ascertain if VC could influence oxidative stress. Twelve healthy males (25 ± 1.4 y) were randomly assigned in a counter-balanced design with a 2-wk period between treatments. Data were analyzed using repeated measures ANOVA. Exercise intensity measures (VO2, RER, RPE, HR, lactate) were similar across treatments. Resting blood oxidative-stress markers were unaffected by treatment. Exercise decreased total blood glutathione (TGSH) and reduced glutathione (GSH) and increased oxidized glutathione (GSSG) (P < 0.01) independent of treatment. Protein carbonyls (PC) increased 3.8 fold in the P (P < 0.01). VC attenuated the PC exercise response in a dose-dependent manner (P < 0.01). Thiobarbituric acid reactive substances (TBARS) was not influenced by exercise (P = 0.68) or VC. These data suggest that VC supplementation can attenuate exercise-induced protein oxidation in a dose-dependent manner with no effect on lipid peroxidation and glutathione status.

Antioxidant Activity of Seabuckthorn (Hippophae rhamnoides) Leaves Extracts on Pork Patties during Refrigerated Storage

2013 ◽  
Vol 781-784 ◽  
pp. 1500-1507
Author(s):  
Zhong Jin ◽  
Qian Liu ◽  
Bao Hua Kong ◽  
Jing Li ◽  
Qian Chen
Keyword(s):  
Ph Value ◽  
Antioxidant Activities ◽  
Thiobarbituric Acid ◽  
Hippophae Rhamnoides ◽  
Protein Carbonyls ◽  
Dependent Manner ◽  
Hippophaë Rhamnoides ◽  
Lipid And Protein Oxidation ◽  
Pork Patties ◽  
Dose Dependent

This experiment was conducted to assess the antioxidant efficacy of Seabuckthorn (Hippophae rhamnoides) leaves extracts (SLE) in raw pork patties during chilled storage at 4 °C for 0-7 days. The pork patties were analyzed periodically for chemical characteristics, such as pH value, colour, thiobarbituric acid-reactive substances value (TBARS), peroxide value (POV) and protein carbonyls content. Compared with the control, SLE treatments significantly decreased the TBARs and POV values and carbonyls formation of pork patties in a dose-dependent manner (P < 0.05), which showed that the SLE significantly inhibited lipid and protein oxidation. The results of this study suggested that the antioxidant activities of seabuckthorn leaves extracts could have great effect on improving the quality characterizes of pork patties and prolong its shelf life.

scholarly journals Influence of lycopene and vitamin C from tomato juice on biomarkers of oxidative stress and inflammation

2007 ◽  
Vol 99 (1) ◽  
pp. 137-146 ◽  
Author(s):  
Karin Jacob ◽  
María J. Periago ◽  
Volker Böhm ◽  
Gaspar Ros Berruezo
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Antioxidant Capacity ◽  
Human Study ◽  
Thiobarbituric Acid ◽  
Tomato Juice ◽  
Protein Carbonyls ◽  
Beneficial Effects ◽  
Lipid Status ◽  
Biomarkers Of Oxidative Stress

A human study was carried out to investigate whether tomato juice, rich in natural lycopene and fortified with vitamin C, is able to reduce several biomarkers of oxidative stress and inflammation and whether the effect can be attributed to lycopene, vitamin C or any other micronutrient. Following a 2-week depletion phase, volunteers were assigned randomly to ingest either tomato juice with (LC) or without (L) vitamin C fortification for 2 weeks (daily dose 20·6 mg lycopene and 45·5/435 mg vitamin C). Plasma and urine were analysed for carotenoids and vitamin C, lipid status, antioxidant capacity, thiobarbituric acid reactive substances (TBARS) and 8-epi-PGF2α, protein carbonyls, cytokines IL-1β and TNFα and C-reactive protein (CRP). The consumption of tomato juice led to a reduction in total cholesterol levels (L: 157·6v. 153·2 mg/dl,P = 0·008; LC: 153·4v. 147·4 mg/dl,P = 0·002) and that of CRP (L: 315·6v. 262·3 μg/l,P = 0·017; LC: 319·2v. 247·1 μg/l,P = 0·001) in both groups. The vitamin C-fortified juice slightly raised the antioxidant capacity in urine and decreased TBARS in plasma and urine. All other markers were affected to a lesser extent or remained unchanged. Cholesterol reduction was correlated with lycopene uptake (P = 0·003), whereas the other effects could not be related with particular micronutrients. Any beneficial effects of tomato consumption for human health cannot be attributed only to lycopene and, as the additional supplementation with ascorbic acid indicates, a variety of antioxidants might be needed to optimize protection against chronic diseases.

scholarly journals Influence of vitamin C supplementation on the oxidative status of rat liver

2008 ◽  
Vol 60 (2) ◽  
pp. 169-173 ◽  
Author(s):  
S.F. Djurasevic ◽  
Jelena Djordjevic ◽  
Tamara Drenca ◽  
N. Jasnic ◽  
Gordana Cvijic
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Rat Liver ◽  
Oxidative Status ◽  
Dependent Manner ◽  
Antioxidative Defense ◽  
Tissue Accumulation ◽  
Vitamin C Supplementation ◽  
Antioxidative Protection ◽  
Dose Dependent

Rats have the ability to synthesize vitamin C in the liver. In the present work, we tested whether its additional intake improves antioxidative protection of the tissue. Thus, we studied the liver?s oxidative status in rats given two supplements of ascorbic acid over a four-week period of time. Our results confirmed that the additional intake of ascorbate improves the liver?s antioxidative defense in a dose-dependent manner. The explanation for the disproportion between the ratio of employed doses of vitamin C and their effects on the studied parameters probably lies in the mechanism of tissue accumulation of ascorbate and balance of its alimentary and endogenous availability.

scholarly journals Indoxyl Sulfate-Induced Oxidative Stress, Mitochondrial Dysfunction, and Impaired Biogenesis Are Partly Protected by Vitamin C and N-Acetylcysteine

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Wen-Chin Lee ◽  
Lung-Chih Li ◽  
Jin-Bor Chen ◽  
Hsueh-Wei Chang
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Mitochondrial Dysfunction ◽  
Umbilical Vein ◽  
Indoxyl Sulfate ◽  
Antiproliferative Effect ◽  
Dependent Manner ◽  
Mitochondrial Dna Copy Number ◽  
C And N ◽  
Dose Dependent

Indoxyl sulfate (IS) contributes to oxidative stress and endothelial dysfunction in chronic kidney disease patients. However, the role of mitochondria in IS-induced oxidative stress is not very clear. In this study, we examined whether mitochondria play a pivotal role in modulating the effects of antioxidants during IS treatment. In the context of human umbilical vein endothelial cells, we found that IS had a dose-dependent antiproliferative effect. In addition, we used flow cytometry to demonstrate that the level of reactive oxygen species increased in a dose-dependent manner after treatment with IS. High doses of IS also corresponded to increased mitochondrial depolarization and decreased mitochondrial DNA copy number and mitochondrial mass. However, these effects could be reversed by the addition of antioxidants, namely, vitamin C and N-acetylcysteine. Thus, our results suggest that IS-induced oxidative stress and antiproliferative effect can be attributed to mitochondrial dysfunction and impaired biogenesis and that these processes can be protected by treatment with antioxidants.

scholarly journals Cardiac Remodeling Induced by All-Trans Retinoic Acid is Detrimental in Normal Rats

2017 ◽  
Vol 43 (4) ◽  
pp. 1449-1459 ◽  
Author(s):  
Renata A. C. Silva ◽  
Andréa F. Gonçalves ◽  
Priscila P. dos Santos ◽  
Bruna Rafacho ◽  
Renan F. T. Claro ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Retinoic Acid ◽  
Energy Metabolism ◽  
Cardiac Remodeling ◽  
Citrate Synthase ◽  
Isolated Heart ◽  
Lipid Hydroperoxide ◽  
Dependent Manner ◽  
Heart Study ◽  
Dose Dependent

Background/Aims: This study aimed to discern whether the cardiac alterations caused by retinoic acid (RA) in normal adult rats are physiologic or pathologic. Methods and Results: Wistar rats were assigned into four groups: control animals (C, n = 20) received a standard rat chow; animals fed a diet supplemented with 0.3 mg/kg/day all-trans-RA (AR1, n = 20); animals fed a diet supplemented with 5 mg/kg/day all-trans-RA (AR2, n = 20); and animals fed a diet supplemented with 10 mg/kg/day all-trans-RA (AR3, n = 20). After 2 months, the animals were submitted to echocardiogram, isolated heart study, histology, energy metabolism status, oxidative stress condition, and the signaling pathway involved in the cardiac remodeling induced by RA. RA increased myocyte cross-sectional area in a dose-dependent manner. The treatment did not change the morphological and functional variables, assessed by echocardiogram and isolated heart study. In contrast, RA changed catalases, superoxide dismutase, and glutathione peroxidases and was associated with increased values of lipid hydroperoxide, suggesting oxidative stress. RA also reduced citrate synthase, enzymatic mitochondrial complex II, ATP synthase, and enzymes of fatty acid metabolism and was associated with increased enzymes involved in glucose use. In addition, RA increased JNK 1/2 expression, without changes in TGF-β, PI3K, AKT, NFκB, S6K, and ERK. Conclusion: In normal rats, RA induces cardiac hypertrophy in a dose-dependent manner. The non-participation of the PI3K/Akt pathway, associated with the participation of the JNK pathway, oxidative stress, and changes in energy metabolism, suggests that cardiac remodeling induced by RA supplementation is deleterious.

Effect of High Dose Vitamin C Supplementation on Muscle Soreness, Damage, Function, and Oxidative Stress to Eccentric Exercise

2006 ◽  
Vol 16 (3) ◽  
pp. 270-280 ◽  
Author(s):  
S.C. Bryer ◽  
A.H. Goldfarb
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Eccentric Exercise ◽  
Muscle Function ◽  
Muscle Soreness ◽  
Damage Function ◽  
High Dose ◽  
Before And After ◽  
Vitamin C Supplementation ◽  
Glutathione Oxidation

This study investigated if vitamin C supplementation before and after eccentric exercise could reduce muscle soreness (MS), oxidative stress, and muscle function. Eighteen healthy men randomly assigned to either a placebo (P) or vitamin C (VC) (3 g/d) treatment group took pills for 2 wk prior and 4 d after performing 70 eccentric elbow extensions with their non-dominant arm. MS increased in both groups with significantly reduced MS for the first 24 h with VC. Range of motion was reduced equally in both groups after the exercise (P ≥ 0.05). Muscle force declined equally and was unaffected by treatment. VC attenuated the creatine kinase (CK) increase at 48 h after exercise with similar CK after this time. Gluta-thione ratio (oxidized glutathione/total glutathione) was significantly increased at 4 and 24 h with P but VC prevented this change. These data suggest that vitamin C pretreatment can reduce MS, delay CK increase, and prevent blood glutathione oxidation with little influence on muscle function loss.

scholarly journals Strawberry-Derived Exosome-Like Nanoparticles Prevent Oxidative Stress in Human Mesenchymal Stromal Cells

Biomolecules ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 87
Author(s):  
Francesca Perut ◽  
Laura Roncuzzi ◽  
Sofia Avnet ◽  
Annamaria Massa ◽  
Nicoletta Zini ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Mammalian Cells ◽  
Dependent Manner ◽  
Potential Health ◽  
Human Mesenchymal Stromal Cells ◽  
Health Promoting ◽  
Size And Morphology

Plant-derived exosome-like nanovesicles (EPDENs) have recently been isolated and evaluated as potential bioactive nutraceutical biomolecules. It has been hypothesized that EPDENs may exert their activity on mammalian cells through their specific cargo. In this study, we isolated and purified EPDENs from the strawberry juice of Fragaria x ananassa (cv. Romina), a new cultivar characterized by a high content of anthocyanins, folic acid, flavonols, and vitamin C and an elevated antioxidant capacity. Fragaria-derived EPDENs were purified by a series of centrifugation and filtration steps. EPDENs showed size and morphology similar to mammalian extracellular nanovesicles. The internalization of Fragaria-derived EPDENs by human mesenchymal stromal cells (MSCs) did not negatively affect their viability, and the pretreatment of MSCs with Fragaria-derived EPDENs prevented oxidative stress in a dose-dependent manner. This is possibly due to the presence of vitamin C inside the nanovesicle membrane. The analysis of EPDEN cargo also revealed the presence of small RNAs and miRNAs. These findings suggest that Fragaria-derived EPDENs may be considered nanoshuttles contained in food, with potential health-promoting activity.

Acute Hypoxia and Exercise-Induced Blood Oxidative Stress

2014 ◽  
Vol 24 (6) ◽  
pp. 684-693 ◽  
Author(s):  
Graham McGinnis ◽  
Brian Kliszczewiscz ◽  
Matthew Barberio ◽  
Christopher Ballmann ◽  
Bridget Peters ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Steady State ◽  
Repeated Measures ◽  
Acute Hypoxia ◽  
Protein Carbonyls ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Lipid Hydroperoxides ◽  
Oxidative Stress Biomarkers ◽  
Post Exercise ◽  
Reducing Ability

Hypoxic exercise is characterized by workloads decrements. Because exercise and high altitude independently elicit redox perturbations, the study purpose was to examine hypoxic and normoxic steady-state exercise on blood oxidative stress. Active males (n = 11) completed graded cycle ergometry in normoxic (975 m) and hypoxic (3,000 m) simulated environments before programing subsequent matched intensity or workload steady-state trials. In a randomized counterbalanced crossover design, participants completed three 60-min exercise bouts to investigate the effects of hypoxia and exercise intensity on blood oxidative stress. Exercise conditions were paired as such; 60% normoxic VO2peak performed in a normoxic environment (normoxic intensity-normoxic environment, NI-NE), 60% hypoxic VO2peak performed in a normoxic environment (HI-NE), and 60% hypoxic VO2peak performed in a hypoxic environment (HI-HE). Blood plasma samples drawn pre (Pre), 0 (Post), 2 (2HR) and 4 (4HR) hr post exercise were analyzed for oxidative stress biomarkers including ferric reducing ability of plasma (FRAP), trolox equivalent antioxidant capacity (TEAC), lipid hydroperoxides (LOOH) and protein carbonyls (PCs). Repeated-measures ANOVA were performed, a priori significance of p ≤ .05. Oxygen saturation during the HI-HE trial was lower than NI-NE and HI-NE (p < .05). A Time × Trial interaction was present for LOOH (p = .013). In the HI-HE trial, LOOH were elevated for all time points post while PC (time; p = .001) decreased post exercise. As evidenced by the decrease in absolute workload during hypoxic VO2peak and LOOH increased during HI-HE versus normoxic exercise of equal absolute (HI-NE) and relative (NI-NE) intensities. Results suggest acute hypoxia elicits work decrements associated with post exercise oxidative stress.

scholarly journals Preclinical Study on the Ameliorating Effect of L-Ascorbic Acid for the Oxidative Stress of Caused by Chronic Administration of Organic Nitrates in Cardiovascular Diseases

2021 ◽  
Author(s):  
Ahmed M Hamdan ◽  
Zuhair M. Mohammedsaleh ◽  
Aalaa Aboelnour ◽  
Sherif M.H. Elkhannishi
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Chronic Administration ◽  
Stress Factors ◽  
Male Rats ◽  
Oxidative Stress Marker ◽  
Organic Nitrates ◽  
Dependent Manner ◽  
Dose Dependent

Abstract PurposeThe therapeutic activity of Glyceryl trinitrate (GTN) is mainly regulated by liberating nitric oxide (NO) and reactive nitrogen species (RNS). During this biotransformation, oxidative stress and lipid peroxidation inside the red blood cells (RBCs) occur. The principal objective of our research is to explain the ameliorating effect of L-ascorbic acid for the deleterious effects of chronic administration of nitrovasodilator drugs. MethodsWe studied some biochemical parameters for the oxidative stress using groups of high sucrose/fat (HSF) diet Wistar male rats chronically orally administered ISMN. Afterwards, we evaluated the role of L-ascorbic acid against these biochemical changes. ResultsChronic treatment with organic nitrates caused elevated serum levels of lipid peroxidation, hemoglobin derivatives as methemoglobin and carboxyhemoglobin, rate of hemoglobin autoxidation, the cellular levels of pro-inflammatory cytokines marker (NF-κB) and apoptosis markers (caspase-3) in myocardium muscles in a dose dependent manner. Meanwhile, such exposure caused decline in the enzymatic effect of superoxide dismutase (SOD), glutathione (GSH) and catalase activity (CAT) accompanied with a decrease of in the level of mitochondrial oxidative stress marker (nrf2) in myocardium muscles and decrease in the serum iron and total iron binding capacity (TIBC) in a dose dependent manner. Concomitant treatment with L-ascorbic acid significantly diminished these changes for all examined parameters.ConclusionChronic administration of organic nitrates leads to the alteration of the level of oxidative stress factors in the myocardium tissue due to generation of reactive oxygen species. Using vitamin C can effectively ameliorate such intoxication to overcome the nitrate tolerance.

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