Host monitoring of quorum sensing during Pseudomonas aeruginosa infection

Pseudomonas aeruginosa rapidly adapts to altered conditions by quorum sensing (QS), a communication system that it uses to collectively modify its behavior through the production, release, and detection of signaling molecules. QS molecules can also be sensed by hosts, although the respective receptors and signaling pathways are poorly understood. We describe a pattern of regulation in the host by the aryl hydrocarbon receptor (AhR) that is critically dependent on qualitative and quantitative sensing of P. aeruginosa quorum. QS molecules bind to AhR and distinctly modulate its activity. This is mirrored upon infection with P. aeruginosa collected from diverse growth stages and with QS mutants. We propose that by spying on bacterial quorum, AhR acts as a major sensor of infection dynamics, capable of orchestrating host defense according to the status quo of infection.

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The roles of small RNAs: insights from bacterial quorum sensing

ExRNA ◽

10.1186/s41544-019-0027-8 ◽

2019 ◽

Vol 1 (1) ◽

Cited By ~ 2

Author(s):

Hongchang Fu ◽

Rosalía Cardós Elena ◽

Paola Handal Marquez

Keyword(s):

Quorum Sensing ◽

Small Rnas ◽

Status Quo ◽

Signaling System ◽

Ribonucleic Acids ◽

Interkingdom Signaling ◽

Bacterial Quorum Sensing ◽

The Status ◽

Non Coding Rnas ◽

Bacterial Quorum

Abstract Ribonucleic acids (RNAs) mainly played auxiliary roles in regulations of genetic processes while recent explorations into small non-coding RNAs (sRNAs) in bacteria have broadened the scope of RNAs studies in these processes. sRNAs have been demonstrated to be involved in various genetic processes and to regulate a variety of bacterial physiologies. Comparatively, quorum sensing (QS) is a mature bacterial cell signaling system which regulates bacteria physiologies as well. Prokaryotic sRNAs studies in the status quo have revealed an emerging picture of trans-kingdom signaling regulation and increasing investigations have demonstrated the feasibility of inter-kingdom signaling as the consequence of QS. We therefore review such phenomena and their similarities to investigate the potential of prokaryote-sourced interkingdom signaling and regulation.

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Faculty Opinions recommendation of Host monitoring of quorum sensing during Pseudomonas aeruginosa infection.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.737114818.793570150 ◽

2020 ◽

Author(s):

Jason Crawford ◽

Emilee Shine

Keyword(s):

Pseudomonas Aeruginosa ◽

Quorum Sensing ◽

Pseudomonas Aeruginosa Infection

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Revisiting Exchange Concept: A Rationale for Marketing Mix in Islamic Marketing

Journal of International Business, Economics and Entrepreneurship ◽

10.24191/jibe.v2i1.14460 ◽

2017 ◽

Vol 2 (1) ◽

pp. 45

Author(s):

Johari Bin Abdullah

Keyword(s):

Literature Review ◽

Focal Point ◽

Marketing Mix ◽

Status Quo ◽

New Paradigm ◽

Qualitative And Quantitative ◽

Islamic Perspective ◽

The Status ◽

Quantitative Approaches

Marketing mix has been the cornerstone of the discipline and dominating marketing early thought. This concept has been the focal point in the development of conventional managerial paradigm with strong adherence to its normative pillars of ‘4 Ps’. History has shown that a new paradigm constantly shifting the status quo of such concept into more advance findings. The objective of this paper is to present the rationale of marketing mix in Islamic marketing. Despite the evolution of marketing mix paradigm over more than 40 years, it is undeniable that such classical marketing canon has its root from concept of exchange. By using both qualitative and quantitative approaches in the development of instruments has led to the development of the 5Cs model. It is aimed to proposed a more advance finding derived from an Islamic perspective towards the concept. In depth literature review and survey has found a distinct set of marketing mix variables catering to Muslim consumer that are in line with Islamic principles. 5Cs models of Commitment, Characteristics, Conformity, Conscience, Customer centric offers a new way of marketing to Muslim consumers governed by Islamic principles and injunctions. Therefore, 5Cs model can be applied in many areas, for instance in Halal industry. It is recommended that business and academia will apply and further develop the model as it will enrich the framework and as to remain relevant.

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Grapefruit essential oils inhibit quorum sensing of Pseudomonas aeruginosa

Food Science and Technology International ◽

10.1177/1082013219883465 ◽

2019 ◽

Vol 26 (3) ◽

pp. 231-241 ◽

Cited By ~ 2

Author(s):

María C Luciardi ◽

M Amparo Blázquez ◽

María R Alberto ◽

Elena Cartagena ◽

Mario E Arena

Keyword(s):

Pseudomonas Aeruginosa ◽

Chemical Composition ◽

Quorum Sensing ◽

Essential Oils ◽

Citrus Paradisi ◽

Food Preservative ◽

Bacterial Quorum Sensing ◽

Citrus Essential Oils ◽

Bacterial Quorum ◽

Cold Pressed

Citrus essential oils are used in food to confer flavor and aromas. The citrus essential oils have been granted as GRAS and could be used as antimicrobial additives to control bacterial quorum sensing from potential food bacterial pathogens. The chemical composition and inhibitory activity of Citrus paradisi (grapefruit) essential oils obtained by cold-pressed method (EOP) and cold-pressed method followed by steam distillation, against Pseudomonas aeruginosa were determined . The GC-MS analyses of the oil indicated the amount of the essential oil components was highest with D-limonene in both cases. However, the extraction method modified the chemical composition. EOP had higher amount of coumarins and flavonoid as well as less oxygenated terpenoids. At 0.1 mg/mL essential oils were not able to modify the bacterial development but inhibited the P. aeruginosa biofilm production between 52% and 55%, sessile viability between 45% and 48%, autoinducer production and elastase activity between 30% and 56%. Limonene was less effective at inhibiting P. aeruginosa than the essential oils, suggesting a synergistic effect of the minor components. According to our results, grapefruit essential oils could be used as a food preservative to control P. aeruginosa virulence.

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Autoinducer Analogs Can Provide Bactericidal Activity to Macrolides in Pseudomonas aeruginosa through Antibiotic Tolerance Reduction

Antibiotics ◽

10.3390/antibiotics11010010 ◽

2021 ◽

Vol 11 (1) ◽

pp. 10

Author(s):

Mizuki Abe ◽

Keiji Murakami ◽

Yuka Hiroshima ◽

Takashi Amoh ◽

Mayu Sebe ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Quorum Sensing ◽

Membrane Permeability ◽

Cell Surface Hydrophobicity ◽

Surface Hydrophobicity ◽

Cell Membrane Permeability ◽

New Combination ◽

Antibiotic Tolerance ◽

Bacterial Quorum Sensing ◽

Bacterial Quorum

Macrolide antibiotics are used in treating Pseudomonas aeruginosa chronic biofilm infections despite their unsatisfactory antibacterial activity, because they display several special activities, such as modulation of the bacterial quorum sensing and immunomodulatory effects on the host. In this study, we investigated the effects of the newly synthesized P. aeruginosa quorum-sensing autoinducer analogs (AIA-1, -2) on the activity of azithromycin and clarithromycin against P. aeruginosa. In the killing assay of planktonic cells, AIA-1 and -2 enhanced the bactericidal ability of macrolides against P. aeruginosa PAO1; however, they did not affect the minimum inhibitory concentrations of macrolides. In addition, AIA-1 and -2 considerably improved the killing activity of azithromycin and clarithromycin in biofilm cells. The results indicated that AIA-1 and -2 could affect antibiotic tolerance. Moreover, the results of hydrocarbon adherence and cell membrane permeability assays suggested that AIA-1 and -2 changed bacterial cell surface hydrophobicity and accelerated the outer membrane permeability of the hydrophobic antibiotics such as azithromycin and clarithromycin. Our study demonstrated that the new combination therapy of macrolides and AIA-1 and -2 may improve the therapeutic efficacy of macrolides in the treatment of chronic P. aeruginosa biofilm infections.

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Alkyne-Substituted Fimbrolide Analogues as Novel Bacterial Quorum-Sensing Inhibitors

Australian Journal of Chemistry ◽

10.1071/ch18194 ◽

2018 ◽

Vol 71 (9) ◽

pp. 708 ◽

Cited By ~ 2

Author(s):

Nripendra Nath Biswas ◽

George M. Iskander ◽

Marcin Mielczarek ◽

Tsz Tin Yu ◽

David StC Black ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Quorum Sensing ◽

Cell Communication ◽

Social Adaptation ◽

Sonogashira Reaction ◽

Coupling Reactions ◽

Bacterial Quorum Sensing ◽

Bacterial Quorum ◽

High Yields ◽

Brominated Furanones

Gram-negative bacteria such as Pseudomonas aeruginosa use furanosyl diesters as autoinducers for quorum sensing (QS), a major regulatory and cell-to-cell communication system for social adaptation, virulence factor production, biofilm formation, and antibiotic resistance. A range of natural and synthetic brominated furanones, i.e. fimbrolide derivatives, have been found to act as inhibitors of QS-dependent bacterial phenotypes, complementing the bactericidal ability of traditional antibiotics. In this work, several novel acetylene analogues of fimbrolides were synthesised in moderate to high yields via Sonogashira coupling reactions of brominated furanones 4-bromo-5-(bromomethylene)furan-2(5H)-one 4 and 5-(dibromomethylene)-3-ethylfuran-2(5H)-one 5. The Sonogashira reaction of acetylenes on 4-bromo-5-(bromomethylene)furan-2(5H)-one 4 was favoured at the C5 methylene bromide over the C4 bromide substituent. On biological testing, the most potent compounds 13 and 14 showed 82 and 98 % bacterial quorum-sensing inhibitory (QSI) activity against Pseudomonas aeruginosa reporter strain respectively.

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Subversive humor in Spanish stand-up comedy

Humor - International Journal of Humor Research ◽

10.1515/humor-2018-0134 ◽

2020 ◽

Vol 33 (1) ◽

pp. 29-54 ◽

Cited By ~ 2

Author(s):

Leonor Ruiz-Gurillo ◽

Esther Linares-Bernabéu

Keyword(s):

Quantitative Analysis ◽

Status Quo ◽

Qualitative And Quantitative Analysis ◽

Qualitative And Quantitative ◽

Stand Up Comedy ◽

Male Power ◽

Linguistic Cues ◽

The Status ◽

Discourse Strategies

AbstractThis paper aims to explore subversive humor in Spanish stand-up comedy by analyzing the work of two well-known Spanish female comedians, Eva Hache and Patricia Sornosa. In order to reach this goal, a corpus of these comedians’ performances has been collected, comprising a total of 25 monologues, which have been divided into humorous sequences, which come to a total of 76 in the corpus of Eva Hache and 37 for Patricia Sornosa. The qualitative and quantitative analysis has focused on subversive humorous sequences, which has shown that only 22.38% of the sequences from Eva Hache’s comic monologues are mainly built around subverting the status quo, whereas Patricia Sornosa challenges the heteronormative discourse in most of her sequences (87.93%). Further, in this case study, we have examined the main linguistic techniques they use when challenging the heteronormative standards, namely the topics, targets, discourse strategies and linguistic cues used to generate a subversive effect. Findings show that both comics use subversive humor but in different ways because of contextual constraints. Whilst Patricia Sornosa offers an overt critique, Eva Hache disparages in a subtler manner even when teasing and undermining male power.

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