scholarly journals Molecular Characterization of Diazotrophic and Denitrifying Bacteria Associated with Mangrove Roots

2007 ◽  
Vol 73 (22) ◽  
pp. 7308-7321 ◽  
Author(s):  
Ana L. Flores-Mireles ◽  
Stephen C. Winans ◽  
Gina Holguin

ABSTRACT An analysis of the molecular diversity of N2 fixers and denitrifiers associated with mangrove roots was performed using terminal restriction length polymorphism (T-RFLP) of nifH (N2 fixation) and nirS and nirK (denitrification), and the compositions and structures of these communities among three sites were compared. The number of operational taxonomic units (OTU) for nifH was higher than that for nirK or nirS at all three sites. Site 3, which had the highest organic matter and sand content in the rhizosphere sediment, as well as the lowest pore water oxygen concentration, had the highest nifH diversity. Principal component analysis of biogeochemical parameters identified soil texture, organic matter content, pore water oxygen concentration, and salinity as the main variables that differentiated the sites. Nonmetric multidimensional scaling (MDS) analyses of the T-RFLP data using the Bray-Curtis coefficient, group analyses, and pairwise comparisons between the sites clearly separated the OTU of site 3 from those of sites 1 and 2. For nirS, there were statistically significant differences in the composition of OTU among the sites, but the variability was less than for nifH. OTU defined on the basis of nirK were highly similar, and the three sites were not clearly separated on the basis of these sequences. The phylogenetic trees of nifH, nirK, and nirS showed that most of the cloned sequences were more similar to sequences from the rhizosphere isolates than to those from known strains or from other environments.

2016 ◽  
Vol 73 (12) ◽  
pp. 1712-1722 ◽  
Author(s):  
Sarah S. Roley ◽  
Jennifer L. Tank

Freshwater mussels are in decline worldwide, but it remains challenging to link specific stressors to mussel declines. The clubshell mussel (Pleurobema clava) is a federally endangered species that spends most of its life completely buried beneath stream sediments. We tested the hypothesis that clubshell’s decline stems, in part, from low pore water dissolved oxygen (DO) concentrations and toxic ammonia (NH3) levels, resulting from sedimentation of interstitial pore spaces. We measured pore water DO, NH3, interstitial sedimentation rates, and sediment organic matter content in the Tippecanoe River (Indiana, USA) at sites that spanned a range of clubshell populations, including two sites devoid of clubshell. We found little evidence for pore water NH3 stress, but pore water DO generally declined with clubshell population and dipped below stress thresholds more frequently at non-clubshell sites than at sites with clubshell. In addition, interstitial sedimentation rates generally increased as clubshell populations declined, suggesting that the low DO concentrations were the result of decreased pore water – surface water exchange. As a result, we conclude that maintaining or improving habitat for clubshell mussels will require the reduction of riverine sediment loading.


Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1567
Author(s):  
Haydee Peña ◽  
Heysa Mendoza ◽  
Fernando Diánez ◽  
Mila Santos

This work studies variables measured from the first phase of composting through the acquisition of the final product, with the goal of identifying those that are more strongly related to quality and are most useful for developing an index. The necessity to establish quality control procedures thus exists for the classification of raw materials in the same way as for the finished products. To accomplish this, three mixtures were prepared, with the goal of achieving a C/N ratio of 30 and a moisture content of 60%. The primary component of each mixture was: fruit processing waste (C1), sewage sludge from the food industry (C2), and the manufacturing waste of fried foods (C3). Temperatures were measured over 107 days, with the corresponding data fit to a logistical model where T °C ~ α / ((1 + exp (− (Time − β) / − γ))) + δ, with interaction compost * time being statistically significant (p < 0.001). This allowed for the temperatures, in keeping with health concerns, to be confirmed. Likewise, a linear regression analysis demonstrated the decomposition of organic matter at 0.82%/week. Statistically, the parameters, measured during the process, with the least variability were selected, which differed in the average contrasts: germination index (cucumber), electrical conductivity, and average moisture. A principal component analysis (PCA) and Spearman’s correlation analysis revealed the best Germination Index (GI) values for C1, due to lower electrical conductivity (EC) and bulk density (Bd) along with higher organic matter content (TOM). For its part, C2 induced a higher Relative emergence (RE) of the cucumber thanks to its higher content of total nitrogen (TN) and lower contribution of Cu, Zn and K. C3 showed a higher presence of salts, less favorable physical characteristics (>Bd and <TPS, total pore space) and higher content of Zn and Cu. Composting carried out with appropriate mixtures can offer high-quality products for use as fertiliser, in soil restoration, and as an alternative substrate to peat and virgin mountain soil.


2017 ◽  
Vol 81 (1) ◽  
pp. 111 ◽  
Author(s):  
Carlos A.M. Barboza ◽  
Tatiana Cabrini ◽  
Gustavo Mattos ◽  
Viviane Skinner ◽  
Ricardo Cardoso

Log-spiral beaches display defined physical gradients alongshore. However, the majority of studies focus on the variability of a single population of macrofauna species. We aimed to investigate the variation in species distribution and in community structure along ten transects on a log-spiral beach. Principal component analysis indicated a clear physical gradient alongshore. Redundancy analysis showed that the sheltered end was related to smaller particle sizes, higher organic matter content and high densities of polychaetes. The exposed end was characterized by coarser sand, lower organic matter content and a high presence of crustaceans. Model selection indicated that the “best fit” to explain the variability in the number of individuals included grain size and beach slope. Variability of the polychaete Scolelepis squamata was best explained by grain size, slope and sediment sorting. The best model for the cirolanid Excirolana armata only included sediment sorting. The physical gradient in sediment texture and the beach slope explained more than one-third of the variability in community structure. The physical variables were also correlated with the distribution of the individual species. We showed that the physical gradient on log-spiral coasts may be an important driver of macrofauna variability, even at mesoscales and in dissipative conditions.


Author(s):  
Sofia Luiza Brito ◽  
Thiago Torres Costa Pereira ◽  
Inez Cristina Martins

Soil degradation is a physical process intensified by political and socioeconomic factors, and by the population growth of the 20th century. In this study, we evaluated the sediments of the Frutal and Bebedouro Streams, located in Frutal municipality, Minas Gerais State, Brazil, and their relation to major human activities that are responsible soil erosion and silting. Samples were collected between 2012 and 2013 for the determination of granulometric composition, organic matter content, and minerals in sediments. The Wentworth scale was used for particle size measurement, and then the size classes regrouped to ABNT scale (NBR 6502/1995). The results found that agriculture and cattle ranching are the main activities that cause intense erosion and silting in both watersheds. Bebedouro Stream is better preserved, as it is completely located in rural area with larger riparian vegetation, unlike the mid-stretch of Frutal Stream, which is located in an urban area. Quartz is the predominant mineral in the sediments as the soils of the region are formed by sandstones; consequently, few nutritional elements were found in the studied samples. Principal component analysis showed that water quality parameters are correlated to silt/clays and organic matter content in most of the collected stations.


Soil Systems ◽  
2020 ◽  
Vol 4 (2) ◽  
pp. 18 ◽  
Author(s):  
Nerea Piñeiro-Juncal ◽  
Carmen Leiva-Dueñas ◽  
Oscar Serrano ◽  
Miguel Ángel Mateo ◽  
Antonio Martínez-Cortízas

Scientists studying seagrasses typically refer to their substratum as sediment, but recently researchers have begun to refer to it as a soil. However, the logistics of sampling underwater substrata and the fragility of these ecosystems challenge their study using pedological methods. Previous studies have reported geochemical processes within the rhizosphere that are compatible with pedogenesis. Seagrass substratum accumulated over the Recent Holocene and can reach several meters in thickness, but studies about deeper layers are scarce. This study is a first attempt to find sound evidence of vertical structuring in Posidonia oceanica deposits to serve as a basis for more detailed pedological studies. A principal component analysis on X-Ray Fluorescence-elemental composition, carbonate content and organic matter content data along a 475 cm core was able to identify four main physico-chemical signals: humification, accumulation of carbonates, texture and organic matter depletion. The results revealed a highly structured deposit undergoing pedogenetical processes characteristic of soils rather than a mere accumulation of sediments. Further research is required to properly describe the substratum underneath seagrass meadows, decide between the sediment or soil nature for seagrass substrata, and for the eventual inclusion of seagrass substrata in soil classifications and the mapping of seagrass soil resources.


Geosciences ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 435
Author(s):  
Sushmita Hossain ◽  
Takashi Ishiyama ◽  
Shoichi Hachinohe ◽  
Chiaki T. Oguchi

The leaching behavior of arsenic (As), lead (Pb), nickel (Ni), iron (Fe), and manganese (Mn) was investigated from subsurface core sediment of marine and nonmarine depositional environments in central Kanto Plain, Japan. A four-step sequential extraction technique was adopted to determine the chemical speciation, potential mobility, and bioavailability of metals under natural conditions in variable depositional environments. In addition, a correlation of these properties with pore water and total metal content was carried out. The concentration of As in pore water was found to be 2–3 times higher than the permissible limit (10 µg/L) for drinking water and leachate in fluvial, transitional, and marine environments. The trend of potential mobile fractions of As, Pb, and Ni showed Fe–Mn oxide bound > carbonate bound > ion exchangeable bound > water soluble in the fluvial environment. However, in the marine environment, it showed Fe–Mn oxide bound > water soluble > carbonate bound > ion exchangeable bound for As. The leaching of As in this fluvial environment is due to the organic matter-mediated, reductive dissolution of Fe–Mn oxide bound, where Mn is the scavenger. The amount of total content of As and sulfur (S) in transitional sediment reflects an elevated level of leachate in pore water, which is controlled by S reduction. However, the leaching of As in marine sediment is controlled by pH and organic matter content.


Author(s):  
O. A. Lipatnikova

The study of heavy metal speciation in bottom sediments of the Vyshnevolotsky water reservoir is presented in this paper. Sequential selective procedure was used to determine the heavy metal speciation in bottom sediments and thermodynamic calculation — to determine ones in interstitial water. It has been shown that Mn are mainly presented in exchangeable and carbonate forms; for Fe, Zn, Pb и Co the forms are related to iron and manganese hydroxides is played an important role; and Cu and Ni are mainly associated with organic matter. In interstitial waters the main forms of heavy metal speciation are free ions for Zn, Ni, Co and Cd, carbonate complexes for Pb, fulvate complexes for Cu. Effects of particle size and organic matter content in sediments on distribution of mobile and potentially mobile forms of toxic elements have been revealed.


2005 ◽  
Vol 33 (1) ◽  
pp. 53-55
Author(s):  
Katalin Berecz ◽  
Katalin Debreczeni

Sign in / Sign up

Export Citation Format

Share Document