scholarly journals Coselection of Cadmium and Benzalkonium Chloride Resistance in Conjugative Transfers from Nonpathogenic Listeria spp. to Other Listeriae

2012 ◽  
Vol 78 (21) ◽  
pp. 7549-7556 ◽  
Author(s):  
S. Katharios-Lanwermeyer ◽  
M. Rakic-Martinez ◽  
D. Elhanafi ◽  
S. Ratani ◽  
J. M. Tiedje ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Benzalkonium Chloride ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Processing Plant ◽  
Cadmium Resistance ◽  
Transfer Resistance ◽  
Content Type ◽  
Plant Environment ◽  
Disinfectant Resistance

ABSTRACTResistance to the quaternary ammonium disinfectant benzalkonium chloride (BC) may be an important contributor to the ability ofListeriaspp. to persist in the processing plant environment. Although a plasmid-borne disinfectant resistance cassette (bcrABC) has been identified inListeria monocytogenes, horizontal transfer of these genes has not been characterized. NonpathogenicListeriaspp. such asL. innocuaandL. welshimeriare more common thanL. monocytogenesin food processing environments and may contribute to the dissemination of disinfectant resistance genes in listeriae, includingL. monocytogenes. In this study, we investigated conjugative transfer of resistance to BC and to cadmium from nonpathogenicListeriaspp. to other nonpathogenic listeriae, as well as toL. monocytogenes. BC-resistantL. welshimeriandL. innocuaharboringbcrABC, along with the cadmium resistance determinantcadA2, were able to transfer resistance to other nonpathogenic listeriae as well as toL. monocytogenesof diverse serotypes, including strains from the 2011 cantaloupe outbreak. Transfer among nonpathogenicListeriaspp. was noticeably higher at 25°C than at 37°C, whereas acquisition of resistance byL. monocytogeneswas equally efficient at 25 and 37°C. When the nonpathogenic donors were resistant to both BC and cadmium, acquisition of cadmium resistance was an effective surrogate for transfer of resistance to BC, suggesting coselection between these resistance attributes. The results suggest that nonpathogenicListeriaspp. may behave as reservoirs for disinfectant and heavy metal resistance genes for other listeriae, including the pathogenic speciesL. monocytogenes.

scholarly journals Draft Genome Sequence of Arthrobacter oryzae TNBS02, a Bacterium Containing Heavy Metal Resistance Genes, Isolated from Soil of Antarctica

2019 ◽  
Vol 8 (4) ◽  
Author(s):  
Yong-Joon Cho ◽  
Ahnna Cho ◽  
Soon Gyu Hong ◽  
Han-Gu Choi ◽  
Ok-Sun Kim
Keyword(s):  
Heavy Metal ◽  
Genome Sequence ◽  
Resistance Genes ◽  
Draft Genome ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Terra Nova Bay ◽  
Victoria Land

Arthrobacter oryzae TNBS02 was isolated from soil at Terra Nova Bay of Victoria Land, Antarctica. The genome consists of a chromosome with 4,248,670 bp which contains a total of 3,994 genes.

scholarly journals Genome Sequence of an Australian Monophasic Salmonella enterica subsp. enterica Typhimurium Isolate (TW-Stm6) Carrying a Large Plasmid with Multiple Antimicrobial Resistance Genes

2017 ◽  
Vol 5 (33) ◽  
Author(s):  
Mike L. Dyall-Smith ◽  
Yuhong Liu ◽  
Helen Billman-Jacobe
Keyword(s):  
Genome Sequence ◽  
Resistance Genes ◽  
Salmonella Enterica ◽  
Heavy Metal Resistance ◽  
Multidrug Resistant ◽  
Metal Resistance ◽  
Large Plasmid ◽  
Content Type ◽  
Antimicrobial Resistance Genes ◽  
Resistant Strains

ABSTRACT We report the genome sequence of a monophasic Salmonella enterica subsp. enterica Typhimurium strain (TW-Stm6) isolated in Australia that is similar to epidemic multidrug-resistant strains from Europe and elsewhere. This strain carries additional antibiotic and heavy-metal resistance genes on a large (275-kb) IncHI2 plasmid.

scholarly journals Genetic Determinants for Cadmium and Arsenic Resistance among Listeria monocytogenes Serotype 4b Isolates from Sporadic Human Listeriosis Patients

2013 ◽  
Vol 79 (7) ◽  
pp. 2471-2476 ◽  
Author(s):  
Sangmi Lee ◽  
M. Rakic-Martinez ◽  
L. M. Graves ◽  
T. J. Ward ◽  
R. M. Siletzky ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Listeria Monocytogenes ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Genetic Determinants ◽  
Chromosomal Locus ◽  
Cadmium Resistance ◽  
Arsenic Resistance ◽  
Content Type ◽  
Serotype 4B

ABSTRACTInListeria monocytogenesserotype 4b isolates from sporadic listeriosis, heavy metal resistance was primarily encountered in certain clonal groups (ECI, ECII, and ECIa). All arsenic-resistant isolates harbored the arsenic resistance cassette previously identified in pLI100; ECIa harbored additional arsenic resistance genes and a novel cadmium resistance determinant in a conserved chromosomal locus.

scholarly journals Distribution of Staphylococcal Cassette Chromosome (SCC)mecElement Types in Fusidic Acid-Resistant Staphylococcus epidermidis and Identification of a Novel SCC7684Element

2016 ◽  
Vol 60 (8) ◽  
pp. 5006-5009 ◽  
Author(s):  
Hsiao-Jan Chen ◽  
Yu-Tzu Lin ◽  
Wei-Chun Hung ◽  
Jui-Chang Tsai ◽  
Po-Ren Hsueh ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Resistance Genes ◽  
Fusidic Acid ◽  
Staphylococcus Epidermidis ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Methicillin Resistant ◽  
Content Type ◽  
Staphylococcal Cassette Chromosome

ABSTRACTWe analyzed the staphylococcal cassette chromosomemec(SCCmec) types of 143 fusidic acid- and methicillin-resistantStaphylococcus epidermidisisolates. The most frequent SCCmectype was SCCmecIII/SCCHg(53%), followed by SCCmecIV (29%). Clonal spreading of SCCmecIII/SCCHgstrains contributed to the increased prevalence of SCCmecIII. A novel non-mecSCC structure, SCC7684, adjacent to SCCmecIII, which carries a newccrCallotype (ccrC3allele 1) and contains heavy metal resistance genes, was identified in 14 isolates.

scholarly journals Metagenomics of Antimicrobial and Heavy Metal Resistance in the Cecal Microbiome of Fattening Pigs Raised without Antibiotics

2021 ◽  
Vol 87 (8) ◽  
Author(s):  
Paiboon Tunsagool ◽  
Wuttichai Mhuantong ◽  
Sithichoke Tangphatsornruang ◽  
Nutthee Am-In ◽  
Rungtip Chuanchuen ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Resistance Genes ◽  
Tetracycline Resistance ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Feed Additives ◽  
Farm Animals ◽  
Content Type ◽  
Fattening Pigs ◽  
Cu And Zn

ABSTRACT This study aimed to assess the cecal microbiome, antimicrobial resistance (AMR), and heavy-metal resistance genes (MRGs) in fattening pigs raised under antibiotic-free (ABF) conditions compared with ordinary industrial pigs (control, C) using whole-genome shotgun sequencing. ABF pigs showed enrichment of Prevotella (33%) and Lactobacillus (13%), whereas Escherichia coli (40%), Fusobacterium, and Bacteroides (each at 4%) were notably observed in the C group. Distinct clusters of cecal microbiota of ABF and C pigs were revealed; however, microbiota of some C pigs (C1) appeared in the same cluster as ABF and were totally separated from the remaining C pigs (C2). For AMR genes, the most abundant genes, tet(Q) (35.7%) and mef(A) (12.7%), were markedly observed in the ABF group, whereas tet(Q) (26.2%) and tet(W) (10.4%) were prominently shown in the C group. tet(Q) was positively correlated with Prevotella in ABF and C1 samples. In the C2 group, the prominent tet(W) was positively correlated with Fusobacterium and Bacteroides. The pigs studied here have never received tetracycline, but pregnant sows received chlortetracycline once every 7 days before parturition. Chromosomal Cu and Zn resistance genes were also shown in both groups regardless of the received Cu and Zn feed additives. A higher abundance of multimetal resistance genes was observed in the C group (44%) than with the ABF group (41%). In conclusion, the microbiome clusters in some C pigs were similar to that in ABF pigs. High-abundance tetracycline resistance genes interrelated to major bacteria were observed in both ABF and C pigs. MRGs were also observed. IMPORTANCE AMR is an increasing problem in farm animals, and raising farm animals without antibiotics is one method that could solve this problem. Our study showed that only some tetracycline and macrolide resistance genes, tet(Q), tet(W), and mef(A), were markedly abundant in ABF and C groups. The tet(Q) and tet(W) genes interrelated to different predominant bacteria in each group, showing the potential role of major bacteria as reservoirs of AMR genes. In addition, chromosomal Cu and Zn resistance genes were also observed in both pig groups, independent of the use of Cu and Zn additives on both farms. The association of MRGs and AMR genotypes and phenotypes, together with the method to resensitize bacteria to antibiotics, should be studied further to unveil the cause of genes conferring high-level resistance and solve these problems.

scholarly journals Heavy Metal Resistance Genes Are Associated withblaNDM-1- andblaCTX-M-15-CarryingEnterobacteriaceae

2018 ◽  
Vol 62 (5) ◽  
pp. e02642-17 ◽  
Author(s):  
Qiu E. Yang ◽  
Siham Rajab Agouri ◽  
Jonathan Mark Tyrrell ◽  
Timothy Rutland Walsh
Keyword(s):  
Heavy Metal ◽  
Resistance Genes ◽  
Gel Electrophoresis ◽  
Heavy Metal Resistance ◽  
Multidrug Resistant ◽  
Metal Resistance ◽  
Pulsed Field Gel Electrophoresis ◽  
High Association ◽  
S1 Nuclease ◽  
Content Type

ABSTRACTThe occurrence of heavy metal resistance genes in multiresistantEnterobacteriaceaepossessingblaNDM-1orblaCTX-M-15genes was examined by PCR and pulsed-field gel electrophoresis with S1 nuclease. Compared with clinical susceptible isolates (10.0% to 30.0%), thepcoA,merA,silC, andarsAgenes occurred with higher frequencies inblaNDM-1-positive (48.8% to 71.8%) andblaCTX-M-15-positive (19.4% to 52.8%) isolates, and they were mostly located on plasmids. Given the high association of metal resistance genes with multidrug-resistantEnterobacteriaceae, increased vigilance needs to be taken with the use of heavy metals in hospitals and the environment.

scholarly journals Diverse Cadmium Resistance Determinants in Listeria monocytogenes Isolates from the Turkey Processing Plant Environment

2009 ◽  
Vol 76 (2) ◽  
pp. 627-630 ◽  
Author(s):  
S. Mullapudi ◽  
R. M. Siletzky ◽  
S. Kathariou
Keyword(s):  
Listeria Monocytogenes ◽  
Benzalkonium Chloride ◽  
Processing Plant ◽  
Cadmium Resistance ◽  
Resistance Determinants ◽  
Plant Environment ◽  
Processing Plants

ABSTRACT Two different cadA cadmium resistance determinants (cadA1, first identified in Tn5422, and cadA2, associated with pLM80) were detected among cadmium-resistant Listeria monocytogenes strains from turkey processing plants. Prevalence of cadA1 versus cadA2 was serotype associated. Cadmium-resistant isolates that were also resistant to benzalkonium chloride (BC) were more likely to harbor cadA2 alone or together with cadA1 than isolates that were cadmium resistant but BC susceptible.

scholarly journals Comparative Genomic Analysis of 450 Strains of Salmonella enterica Isolated from Diseased Animals

Genes ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 1025
Author(s):  
Shaohua Zhao ◽  
Cong Li ◽  
Chih-Hao Hsu ◽  
Gregory H. Tyson ◽  
Errol Strain ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Bacterial Infections ◽  
Genomic Analysis ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Comparative Genomic ◽  
Salmonella Infections ◽  
Antimicrobial Resistance Genes ◽  
Salmonella Pathogenicity Islands ◽  
Shed Light

Salmonella is a leading cause of bacterial infections in animals and humans. We sequenced a collection of 450 Salmonella strains from diseased animals to better understand the genetic makeup of their virulence and resistance features. The presence of Salmonella pathogenicity islands (SPIs) varied by serotype. S. Enteritidis carried the most SPIs (n = 15), while S. Mbandaka, S. Cerro, S. Meleagridis, and S. Havana carried the least (n = 10). S. Typhimurium, S. Choleraesuis, S. I 4,5,12:i:-, and S. Enteritidis each contained the spv operon on IncFII or IncFII-IncFIB hybrid plasmids. Two S. IIIa carried a spv operon with spvD deletion on the chromosome. Twelve plasmid types including 24 hybrid plasmids were identified. IncA/C was frequently associated with S. Newport (83%) and S. Agona (100%) from bovine, whereas IncFII (100%), IncFIB (100%), and IncQ1 (94%) were seen in S. Choleraesuis from swine. IncX (100%) was detected in all S. Kentucky from chicken. A total of 60 antimicrobial resistance genes (ARGs), four disinfectant resistances genes (DRGs) and 33 heavy metal resistance genes (HMRGs) were identified. The Salmonella strains from sick animals contained various SPIs, resistance genes and plasmid types based on the serotype and source of the isolates. Such complicated genomic structures shed light on the strain characteristics contributing to the severity of disease and treatment failures in Salmonella infections, including those causing illnesses in animals.

scholarly journals Retromobilization of heavy metal resistance genes in unpolluted and heavy metal polluted soil

1995 ◽  
Vol 18 (3) ◽  
pp. 191-203 ◽  
Author(s):  
Eva M. Top ◽  
Helene Rore ◽  
Jean-Marc Collard ◽  
Veerle Gellens ◽  
Galina Slobodkina ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Resistance Genes ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Polluted Soil

scholarly journals Target (MexB)- and Efflux-Based Mechanisms Decreasing the Effectiveness of the Efflux Pump Inhibitor D13-9001 in Pseudomonas aeruginosa PAO1: Uncovering a New Role for MexMN-OprM in Efflux of β-Lactams and a Novel Regulatory Circuit (MmnRS) Controlling MexMN Expression

2018 ◽  
Vol 63 (2) ◽  
pp. e01718-18 ◽  
Author(s):  
Srijan Ranjitkar ◽  
Adriana K. Jones ◽  
Mina Mostafavi ◽  
Zachary Zwirko ◽  
Oleg Iartchouk ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Efflux Pump ◽  
Response Regulator ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Rna Seq ◽  
Efflux Pump Inhibitor ◽  
Content Type ◽  
Regulatory Circuit ◽  
Outer Membrane Channel

ABSTRACT Efflux pumps contribute to antibiotic resistance in Gram-negative pathogens. Correspondingly, efflux pump inhibitors (EPIs) may reverse this resistance. D13-9001 specifically inhibits MexAB-OprM in Pseudomonas aeruginosa. Mutants with decreased susceptibility to MexAB-OprM inhibition by D13-9001 were identified, and these fell into two categories: those with alterations in the target MexB (F628L and ΔV177) and those with an alteration in a putative sensor kinase of unknown function, PA1438 (L172P). The alterations in MexB were consistent with reported structural studies of the D13-9001 interaction with MexB. The PA1438L172P alteration mediated a >150-fold upregulation of MexMN pump gene expression and a >50-fold upregulation of PA1438 and the neighboring response regulator gene, PA1437. We propose that these be renamed mmnR and mmnS for MexMN regulator and MexMN sensor, respectively. MexMN was shown to partner with the outer membrane channel protein OprM and to pump several β-lactams, monobactams, and tazobactam. Upregulated MexMN functionally replaced MexAB-OprM to efflux these compounds but was insusceptible to inhibition by D13-9001. MmnSL172P also mediated a decrease in susceptibility to imipenem and biapenem that was independent of MexMN-OprM. Expression of oprD, encoding the uptake channel for these compounds, was downregulated, suggesting that this channel is also part of the MmnSR regulon. Transcriptome sequencing (RNA-seq) of cells encoding MmnSL172P revealed, among other things, an interrelationship between the regulation of mexMN and genes involved in heavy metal resistance.

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