scholarly journals Dynamics of Salmonella Shedding and Welfare of Hens in Free-Range Egg Production Systems

2016 ◽  
Vol 83 (5) ◽  
Author(s):  
Vaibhav C. Gole ◽  
Rebecca Woodhouse ◽  
Charles Caraguel ◽  
Talia Moyle ◽  
Jean-Loup Rault ◽  
...  
Keyword(s):  
Production System ◽  
Salmonella Enterica ◽  
Egg Production ◽  
Production Systems ◽  
Most Probable Number ◽  
Probable Number ◽  
Content Type ◽  
Free Range ◽  
Phage Types ◽  
Positive Correlations

ABSTRACT The current study investigated the effect of environmental stressors (i.e., weather changes) on Salmonella shedding in free-range production systems and the correlations with behavioral and physiological measures (i.e., fecal glucocorticoid metabolites). This involved longitudinal and point-in-time surveys of Salmonella shedding and environmental contamination on four commercial free-range layer farms. The shedding of Salmonella was variable across free-range farms and in different seasons. There was no significant effect of season on the Salmonella prevalence during this investigation. In this study, the combined Salmonella most probable number (MPN) counts in environmental (including feces, egg belt, dust, nest box, and ramp) samples were highest in samples collected during the summer season (4th sampling, performed in February). The predominant serovars isolated during this study were Salmonella enterica serovar Mbandaka and Salmonella enterica serovar Typhimurium phage types 135 and 135a. These two phage types were involved in several egg product-related Salmonella outbreaks in humans. Multilocus variable-number tandem-repeat analysis (MLVA) results indicated that MLVA types detected from human food poisoning cases exhibited MLVA patterns similar to the strains isolated during this study. All Salmonella isolates (n = 209) were tested for 15 different genes involved in adhesion, invasion, and survival of Salmonella spp. We also observed variations for sopA, ironA, and misL. There were no positive correlations between fecal corticosterone metabolite (FCM) and Salmonella prevalence and/or shedding in feces. Also, there were no positive correlations between Salmonella prevalence and Salmonella count (log MPN) and any of the other welfare parameters. IMPORTANCE In this study, the welfare of laying hens and Salmonella shedding were compared over a prolonged period of time in field conditions. This study investigated the long-term shedding of Salmonella serovars in a free-range egg production system. Given that there is increasing demand for free-range eggs, it is essential to understand the risks associated with such a production system.

scholarly journals Distribution and Characterization of Salmonella enterica Isolates from Irrigation Ponds in the Southeastern United States

2015 ◽  
Vol 81 (13) ◽  
pp. 4376-4387 ◽  
Author(s):  
Zhiyao Luo ◽  
Ganyu Gu ◽  
Amber Ginn ◽  
Mihai C. Giurcanu ◽  
Paige Adams ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Geometric Mean ◽  
Multidrug Resistant ◽  
Most Probable Number ◽  
Sample Type ◽  
Probable Number ◽  
Content Type ◽  
Safety Standards ◽  
Produce Contamination ◽  
Southern Georgia

ABSTRACTIrrigation water has been implicated as a likely source of produce contamination bySalmonella enterica. Therefore, the distribution ofS. entericawas surveyed monthly in irrigation ponds (n= 10) located within a prime agricultural region in southern Georgia and northern Florida. All ponds and 28.2% of all samples (n= 635) were positive forSalmonella, with an overall geometric mean concentration (0.26 most probable number [MPN]/liter) that was relatively low compared to prior reports for rivers in this region.Salmonellapeaks were seasonal; the levels correlated with increased temperature and rainfall (P< 0.05). The numbers and occurrence were significantly higher in water (0.32 MPN/liter and 37% of samples) than in sediment (0.22 MPN/liter and 17% of samples) but did not vary with depth. Representative isolates (n= 185) from different ponds, sample types, and seasons were examined for resistance to 15 different antibiotics; most strains were resistant to streptomycin (98.9%), while 20% were multidrug resistant (MDR) for 2 to 6 antibiotics. DiversiLab repetitive extragenic palindromic-element sequence-based PCR (rep-PCR) revealed genetic diversity and showed 43 genotypes among 191 isolates, as defined by >95% similarity. The genotypes did not partition by pond, season, or sample type. Genetic similarity to known serotypes indicated Hadar, Montevideo, and Newport as the most prevalent. All ponds achieved the current safety standards for genericEscherichia coliin agricultural water, and regression modeling showed that theE. colilevel was a significant predictor for the probability ofSalmonellaoccurrence. However, persistent populations ofSalmonellawere widely distributed in irrigation ponds, and the associated risks for produce contamination and subsequent human exposure are unknown, supporting continued surveillance of this pathogen in agricultural settings.

Persistence of Orally Administered Salmonella enterica Serovars Agona and Montevideo in Atlantic Salmon (Salmo salar L.)

2005 ◽  
Vol 68 (7) ◽  
pp. 1336-1339 ◽  
Author(s):  
L. L. NESSE ◽  
T. LØVOLD ◽  
B. BERGSJØ ◽  
K. NORDBY ◽  
C. WALLACE ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Atlantic Salmon ◽  
Salmo Salar ◽  
Salmonella Enterica ◽  
Most Probable Number ◽  
Fish Feed ◽  
Internal Organs ◽  
Probable Number ◽  
Atlantic Salmon Salmo Salar ◽  
Gastric Intubation

The objective of our experiments was to study the persistence and dissemination of orally administered Salmonella in smoltified Atlantic salmon. In experiment 1, salmon kept at 15°C were fed for 1 week with feed contaminated with 96 most-probable-number units of Salmonella Agona per 100 g of feed and then starved for 2 weeks. Samples were taken from the gastrointestinal tract and examined for Salmonella 1, 2, 8, 9, 15, and 16 days after the feeding ended. In experiment 2, Salmonella Agona and Montevideo were separately mixed with feed and administered by gastric intubation. Each fish received 1.0 × 108, 1.0 × 106, or 1.0 × 104 CFU. The different groups were kept in parallel at 5 and 15°C and observed for 4 weeks. Every week, three fish in each group were sacrificed, and samples were taken from the skin, the pooled internal organs, the muscle, and the gastrointestinal tract and examined for the presence of Salmonella. The results from the two experiments showed that the persistence of Salmonella in the fish was highly dependent on the dose administered. Salmonella was not recovered from any of the fish that were fed for 1 week with the lowest concentration of Salmonella. In the fish given the highest dose of Salmonella, bacteria persisted for at least 4 weeks in the gastrointestinal tract as well as, to some extent, the internal organs. The present study shows that under practical conditions in Norway, the risk of Salmonella in fish feed being passed on to the consumer of the fish is negligible.

System dynamics modelling of fixed and dynamic Kanban controlled production systems: a supply chain perspective

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Jagan Mohan Reddy K. ◽  
Neelakanteswara Rao A. ◽  
Krishnanand Lanka ◽  
PRC Gopal
Keyword(s):  
Supply Chain ◽  
System Dynamics ◽  
Cycle Time ◽  
Production System ◽  
Demand Uncertainty ◽  
Production Systems ◽  
Decision Variable ◽  
Content Type ◽  
Pull Production ◽  
Kanban System

Purpose Pull production systems have received much attention in the supply chain management environment. The number of Kanbans is a key decision variable in the pull production system as it affects the finished goods inventory (FGI) and backorders of the system. The purpose of this study is to compare the performance of the fixed and dynamic Kanban systems in terms of operational metrics (FGI and backorders) under the demand uncertainty. Design/methodology/approach In this paper, the system dynamics (SD) approach was used to model the performance of fixed and dynamic Kanban based production systems. SD approach has enabled the feedback mechanism and is an appropriate tool to incorporate the dynamic control during the simulation. Initially, a simple Kanban based production system was developed and then compared the performance of production systems with fixed and dynamic controlled Kanbans at the various demand scenarios. Findings From the present study, it is observed that the dynamic Kanban system has advantages over the fixed Kanban system and also observed that the variation in the backorders with respect to the demand uncertainty under the dynamic Kanban system is negligible. Research limitations/implications In a just-in-time production system, the number of Kanbans is a key decision variable. The number of Kanbans is mainly depended on the demand, cycle time, safety stock factor (SSF) and container size. However, this study considered only demand uncertainty to compare the fixed and dynamic Kanban systems. This paper further recommends researchers to consider other control variables which may influence the number of Kanbans such as cycle time, SSF and container size. Originality/value This study will be useful to decision-makers and production managers in the selection of the Kanban systems in uncertain demand applications.

scholarly journals Virulence and Metabolic Characteristics of Salmonella enterica Serovar Enteritidis Strains with DifferentsefDVariants in Hens

2012 ◽  
Vol 78 (18) ◽  
pp. 6405-6412 ◽  
Author(s):  
Cesar A. Morales ◽  
Jean Guard ◽  
Roxana Sanchez-Ingunza ◽  
Devendra H. Shah ◽  
Mark Harrison
Keyword(s):  
Salmonella Enterica ◽  
Egg Production ◽  
The Body ◽  
Wild Type ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Blood Calcium ◽  
Content Type ◽  
Metabolic Characteristics ◽  
Wide Range ◽  
Metabolic Properties

ABSTRACTSalmonella entericaserovar Enteritidis is one of a fewSalmonella entericaserotypes that has SEF14 fimbriae encoded by thesefoperon, which consists of 4 cotranscribed genes,sefABCD, regulated bysefR. A parental strain was used to construct asefDmutant and its complement, and all 3 strains were compared for gene expression, metabolic properties, and virulence characteristics in hens. Transcription ofsefDby wild type was suppressed at 42°C and absent for the mutant under conditions where the complemented mutant had 103times higher transcription. Growth of the complemented mutant was restricted in comparison to that of the mutant and wild type. Hens infected with the wild type and mutant showed decreased blood calcium and egg production, but infection with the complemented mutant did not. Thus, the absence ofsefDcorrelated with increased metabolic capacity and enhanced virulence of the pathogen. These results suggest that any contribution thatsefDmakes to egg contamination is either unknown or would be limited to early transmission from the environment to the host. Absence ofsefD, either through mutation or by suppression of transcription at the body temperature of the host, may contribute to the virulence ofSalmonella entericaby facilitating growth on a wide range of metabolites.

scholarly journals On data leakage from non-production systems

2017 ◽  
Vol 25 (4) ◽  
pp. 454-474
Author(s):  
Jacqueline Cope ◽  
Francois Siewe ◽  
Feng Chen ◽  
Leandros Maglaras ◽  
Helge Janicke
Keyword(s):  
Production System ◽  
Production Systems ◽  
Human Interaction ◽  
Production Data ◽  
Business Systems ◽  
Sensitive Data ◽  
Process Flow ◽  
Content Type ◽  
Practical Solution ◽  
Production Environments

Purpose This study is an exploration of areas pertaining to the use of production data in non-production environments. During the software development life cycle, non-production environments are used to serve various purposes to include unit, component, integration, system, user acceptance, performance and configuration testing. Organisations and third parties have been and are continuing to use copies of production data in non-production environments. This can lead to personal and sensitive data being accidentally leaked if appropriate and rigorous security guidelines are not implemented. This paper aims to propose a comprehensive framework for minimising data leakage from non-production environments. The framework was evaluated using guided interviews and was proven effective in helping organisation manage sensitive data in non-production environments. Design/methodology/approach Authors conducted a thorough literature review on areas related to data leakage from non-production systems. By doing an analysis of advice, guidelines and frameworks that aims at finding a practical solution for selecting and implementing a de-identification solution of sensitive data, the authors managed to highlight the importance of all areas related to sensitive data protection. Based on these areas, a framework was proposed which was evaluated by conducting set of guided interviews. Findings This paper has researched the background information and produced a framework for an organisation to manage sensitive data in its non-production environments. This paper presents a proposed framework that describes a process flow from the legal and regulatory requirements to data treatment and protection, gained through understanding the organisation’s business, the production system, the purpose and the requirements of the non-production environment. The paper shows that there is some conflict between security and perceived usability, which may be addressed by challenging the perceptions of usability or identifying the compromise required. Non-production environments need not be the sole responsibility of the IT section, they should be of interest to the business area that is responsible for the data held. Originality/value This paper proposes a simplified business model and framework. The proposed model diagrammatically describes the interactions of elements affecting the organisation. It highlights how non-production environments may be perceived as separate from the business systems, but despite the perceptions, these are still subject to the same legal requirements and constraints. It shows the interdependency of data, software, technical infrastructure and human interaction and how the change of one element may affect the others. The proposed framework describes the process flow and forms a practical solution in assisting the decision-making process and providing documentary evidence for assurance and audit purposes. It looks at the requirements of the non-production system in relation to the legal and regulatory constraints, as well as the organisational requirements and business systems. The impact of human factors on the data is also considered to bring a holistic approach to the protection of non-production environments.

scholarly journals Effects of production system and feeding regimen on carcass and meat quality traits of Naked Neck chicken

2021 ◽  
Vol 51 (2) ◽  
pp. 250-261
Author(s):  
E. Bughio ◽  
J. Hussain ◽  
A. Mahmud ◽  
A. Khalique
Keyword(s):  
Meat Quality ◽  
Production System ◽  
Production Systems ◽  
Liver Weight ◽  
Kitchen Waste ◽  
Naked Neck ◽  
Commercial Diet ◽  
Free Range ◽  
Commercial Feed ◽  
Feeding Regimen

To evaluate the effect of a production system and feeding regimen on meat quality attributes of Naked Neck chickens, a total of 150 cockerels at 18 weeks old (1625 ± 70 g) were collected from 10 treatment groups with five replicates of three birds. The factorial arrangement of treatments consisted of two production systems (intensive and free-range) and five nutritional regimens, namely 100% commercial feed; 75% commercial feed plus 25% kitchen waste; 50% commercial feed plus 50% kitchen waste; 25% commercial feed plus 75% kitchen waste; and 100% kitchen waste. Carcass traits, meat quality, and meat organoleptic were found to differ significantly among production systems, feeding regimens, and their interaction. Higher liver weight was observed in birds reared under an intensive system. Higher gizzard weight was noted in birds fed with 100% kitchen waste, whereas lower gizzard weight was observed in birds fed the commercial diet. The meat from cockerels fed with 75% kitchen waste was most yellow, whereas the meat from the birds fed with 100% kitchen waste was least yellow. At two hours after slaughter, pH of the meat was highest in birds fed 50% kitchen waste and lowest in birds fed 100% kitchen waste. The interaction of production system and feeding regimen was significant for overall acceptability score. In conclusion, Naked Neck chickens performed equally well under intensive and free-range systems, irrespective of the level of kitchen waste that they were fed.

scholarly journals Long-Term Study of Vibrio parahaemolyticus Prevalence and Distribution in New Zealand Shellfish

2015 ◽  
Vol 81 (7) ◽  
pp. 2320-2327 ◽  
Author(s):  
C. D. Cruz ◽  
D. Hedderley ◽  
G. C. Fletcher
Keyword(s):  
New Zealand ◽  
Vibrio Parahaemolyticus ◽  
Most Probable Number ◽  
Pcr Analysis ◽  
Potential Hazard ◽  
Probable Number ◽  
Content Type ◽  
The North ◽  
Long Term Study ◽  
Food Borne

ABSTRACTThe food-borne pathogenVibrio parahaemolyticushas been reported as being present in New Zealand (NZ) seawaters, but there have been no reported outbreaks of food-borne infection from commercially grown NZ seafood. Our study determined the current incidence ofV. parahaemolyticusin NZ oysters and Greenshell mussels and the prevalence ofV. parahaemolyticustdhandtrhstrains. Pacific (235) and dredge (21) oyster samples and mussel samples (55) were obtained from commercial shellfish-growing areas between December 2009 and June 2012. TotalV. parahaemolyticusnumbers and the presence of pathogenic genestdhandtrhwere determined using the FDA most-probable-number (MPN) method and confirmed using PCR analysis. In samples from the North Island of NZ,V. parahaemolyticuswas detected in 81% of Pacific oysters and 34% of mussel samples, while the numbers ofV. parahaemolyticustdhandtrhstrains were low, with just 3/215 Pacific oyster samples carrying thetdhgene.V. parahaemolyticusorganisms carryingtdhandtrhwere not detected in South Island samples, andV. parahaemolyticuswas detected in just 1/21 dredge oyster and 2/16 mussel samples. Numbers ofV. parahaemolyticusorganisms increased when seawater temperatures were high, the season when most commercial shellfish-growing areas are not harvested. The numbers ofV. parahaemolyticusorganisms in samples exceeded 1,000 MPN/g only when the seawater temperatures exceeded 19°C, so this environmental parameter could be used as a trigger warning of potential hazard. There is some evidence that the totalV. parahaemolyticusnumbers increased compared with those reported from a previous 1981 to 1984 study, but the analytical methods differed significantly.

scholarly journals Repeated Anaerobic Microbial Redox Cycling of Iron

2011 ◽  
Vol 77 (17) ◽  
pp. 6036-6042 ◽  
Author(s):  
Aaron J. Coby ◽  
Flynn Picardal ◽  
Evgenya Shelobolina ◽  
Huifang Xu ◽  
Eric E. Roden
Keyword(s):  
Microbial Communities ◽  
Microscopic Analysis ◽  
Redox Cycling ◽  
Most Probable Number ◽  
Rrna Gene ◽  
Electron Microscopic ◽  
16S Rrna Gene Analysis ◽  
Probable Number ◽  
Content Type ◽  
Floodplain Sediments

ABSTRACTSome nitrate- and Fe(III)-reducing microorganisms are capable of oxidizing Fe(II) with nitrate as the electron acceptor. This enzymatic pathway may facilitate the development of anaerobic microbial communities that take advantage of the energy available during Fe-N redox oscillations. We examined this phenomenon in synthetic Fe(III) oxide (nanocrystalline goethite) suspensions inoculated with microflora from freshwater river floodplain sediments. Nitrate and acetate were added at alternate intervals in order to induce repeated cycles of microbial Fe(III) reduction and nitrate-dependent Fe(II) oxidation. Addition of nitrate to reduced, acetate-depleted suspensions resulted in rapid Fe(II) oxidation and accumulation of ammonium. High-resolution transmission electron microscopic analysis of material from Fe redox cycling reactors showed amorphous coatings on the goethite nanocrystals that were not observed in reactors operated under strictly nitrate- or Fe(III)-reducing conditions. Microbial communities associated with N and Fe redox metabolism were assessed using a combination of most-probable-number enumerations and 16S rRNA gene analysis. The nitrate-reducing and Fe(III)-reducing cultures were dominated by denitrifyingBetaproteobacteria(e.g.,Dechloromonas) and Fe(III)-reducingDeltaproteobacteria(Geobacter), respectively; these same taxa were dominant in the Fe cycling cultures. The combined chemical and microbiological data suggest that bothGeobacterand variousBetaproteobacteriaparticipated in nitrate-dependent Fe(II) oxidation in the cycling cultures. Microbially driven Fe-N redox cycling may have important consequences for both the fate of N and the abundance and reactivity of Fe(III) oxides in sediments.

scholarly journals The Effects of Production System and Sex on the Sensory Quality Characteristics of Dorper Lamb

Foods ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 725
Author(s):  
Louwrens Christiaan Hoffman ◽  
Bianca Claasen ◽  
Daniël André Van der Merwe ◽  
Schalk Willem Petrus Cloete ◽  
Jasper Johannes Erasmus Cloete
Keyword(s):  
Production System ◽  
Sensory Quality ◽  
Production Systems ◽  
Quality Characteristics ◽  
Free Range ◽  
Ram Lambs ◽  
Feedlot Lambs ◽  
Pelleted Diet ◽  
Group 2 ◽  
Group 1

The effect of production systems on the sensory quality characteristics of Dorper lambs was investigated. Sixty lambs (ewes, rams, castrates) were allocated into two production groups (feedlot or free-range) at weaning with equal numbers of each sex represented in each group. The lambs were fed for five (slaughter group 1) or six (slaughter group 2) weeks. Feedlot lambs were fed a commercial pelleted diet while free-range lambs utilized natural shrub pastures. Samples of the Longissimus thoracis muscle were used for sensory evaluation. Feedlot lambs produced meat that was juicier and more tender than meat from free-range lambs. Initial juiciness was also higher in the meat from the feedlot lambs. No aroma or flavour differences were observed. The meat from the free-range ram lambs (slaughter group 1) was the least tender, whereas the lamb flavour was also compromised in the free-range ram lambs. Free-range meat may not necessarily be distinguished from feedlot meat as far as aroma and flavour are concerned.

scholarly journals Whole-Genome Sequencing of Salmonella enterica Serovar Infantis and Kentucky Isolates Obtained from Layer Poultry Farms in Ecuador

2020 ◽  
Vol 9 (13) ◽  
Author(s):  
William Calero-Cáceres ◽  
Joyce Villacís ◽  
Maria Ishida ◽  
Elton Burnett ◽  
Christian Vinueza-Burgos
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Salmonella Enterica ◽  
Egg Production ◽  
Illumina Miseq ◽  
Whole Genome ◽  
Genome Sequences ◽  
Content Type ◽  
Poultry Farms

Five strains of Salmonella enterica subsp. enterica serovar Infantis and two strains of S. enterica subsp. enterica serovar Kentucky isolated in 2017 from Ecuadorian layer poultry farms were sequenced using Illumina MiSeq technology. These isolates were collected on layer farms in central Ecuador, one of the most important areas of egg production in the country. The genome sequences of these isolates show valuable information for surveillance purposes.

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