scholarly journals Mycobacterium gilvum Illustrates Size-Correlated Relationships between Mycobacteria and Acanthamoeba polyphaga

2012 ◽  
Vol 79 (5) ◽  
pp. 1606-1611 ◽  
Author(s):  
Otmane Lamrabet ◽  
Michel Drancourt
Keyword(s):  
Electron Microscopy ◽  
Experimental Study ◽  
Acanthamoeba Polyphaga ◽  
Free Living ◽  
Content Type ◽  
Rapidly Growing Mycobacteria ◽  
Optical And Electron Microscopy ◽  
Negative Controls ◽  
Soil And Water

ABSTRACTMycobacteria are isolated from soil and water environments, where free-living amoebae live. Free-living amoebae are bactericidal, yet some rapidly growing mycobacteria are amoeba-resistant organisms that survive in the amoebal trophozoites and cysts. Such a capacity has not been studied for the environmental rapidly growing organismMycobacterium gilvum. We investigated the ability ofM. gilvumto survive in the trophozoites ofAcanthamoeba polyphagastrain Linc-AP1 by using optical and electron microscopy and culture-based microbial enumerations in the presence of negative controls. We observed that 29% ofA. polyphagacells were infected byM. gilvummycobacteria by 6 h postinfection. SurvivingM. gilvummycobacteria did not multiply and did not kill the amoebal trophozoites during a 5-day coculture. Extensive electron microscopy observations indicated thatM. gilvummeasured 1.4 ± 0.5 μm and failed to findM. gilvumorganisms in the amoebal cysts. Further experimental study of two other rapidly growing mycobacteria,Mycobacterium rhodesiaeandMycobacterium thermoresistibile, indicated that both measured <2 μm and exhibited the same amoeba-mycobacterium relationships asM. gilvum. In general, we observed that mycobacteria measuring <2 μm do not significantly grow within and do not kill amoebal trophozoites, in contrast to mycobacteria measuring >2 μm (P< 0.05). The mechanisms underlying such an observation remain to be determined.

scholarly journals Cellular Response of the Amoeba Acanthamoeba castellanii to Chlorine, Chlorine Dioxide, and Monochloramine Treatments

2011 ◽  
Vol 77 (14) ◽  
pp. 4974-4980 ◽  
Author(s):  
Emerancienne Mogoa ◽  
Charles Bodet ◽  
Franck Morel ◽  
Marie-Hélène Rodier ◽  
Bernard Legube ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Flow Cytometry ◽  
Chlorine Dioxide ◽  
Mode Of Action ◽  
Cellular Response ◽  
Acanthamoeba Castellanii ◽  
Size Reduction ◽  
Resistant Bacteria ◽  
Free Living ◽  
Content Type

ABSTRACTAcanthamoeba castellaniiis a free-living amoebae commonly found in water systems. Free-living amoebae might be pathogenic but are also known to bear phagocytosis-resistant bacteria, protecting these bacteria from water treatments. The mode of action of these treatments is poorly understood, particularly on amoebae. It is important to examine the action of these treatments on amoebae in order to improve them. The cellular response to chlorine, chlorine dioxide, and monochloramine was tested onA. castellaniitrophozoites. Doses of disinfectants leading to up to a 3-log reduction were compared by flow cytometry and electron microscopy. Chlorine treatment led to size reduction, permeabilization, and retraction of pseudopods. In addition, treatment with chlorine dioxide led to a vacuolization of the cytoplasm. Monochloramine had a dose-dependent effect. At the highest doses monochloramine treatment resulted in almost no changes in cell size and permeability, as shown by flow cytometry, but the cell surface became smooth and dense, as seen by electron microscopy. We show that these disinfectants globally induced size reduction, membrane permeabilization, and morphological modifications but that they have a different mode of action onA. castellanii.

scholarly journals Acanthamoeba polyphaga Mimivirus Prevents Amoebal Encystment-Mediating Serine Proteinase Expression and Circumvents Cell Encystment

2014 ◽  
Vol 89 (5) ◽  
pp. 2962-2965 ◽  
Author(s):  
Paulo Boratto ◽  
Jonas Dutra Albarnaz ◽  
Gabriel Magno de Freitas Almeida ◽  
Lucas Botelho ◽  
Alide Caroline Lima Fontes ◽  
...  
Keyword(s):  
Serine Proteinase ◽  
Giant Virus ◽  
Acanthamoeba Polyphaga ◽  
Free Living ◽  
Content Type ◽  
Acanthamoeba Polyphaga Mimivirus

Acanthamoebais a genus of free-living amoebas distributed worldwide. Few studies have explored the interactions between these protozoa and their infecting giant virus,Acanthamoeba polyphagamimivirus (APMV). Here we show that, once the amoebal encystment is triggered, trophozoites become significantly resistant to APMV. Otherwise, upon infection, APMV is able to interfere with the expression of a serine proteinase related to amoebal encystment and the encystment can no longer be triggered.

scholarly journals Reduction of Legionella spp. in Water and in Soil by a Citrus Plant Extract Vapor

2014 ◽  
Vol 80 (19) ◽  
pp. 6031-6036 ◽  
Author(s):  
Katie Laird ◽  
Elena Kurzbach ◽  
Jodie Score ◽  
Jyoti Tejpal ◽  
George Chi Tangyie ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Water System ◽  
Severe Form ◽  
Gas Chromatography Mass Spectrometry ◽  
Free Living ◽  
Content Type ◽  
Soil Systems ◽  
Viable Cells ◽  
Legionnaires Disease ◽  
Soil And Water

ABSTRACTLegionnaires' disease is a severe form of pneumonia caused byLegionellaspp., organisms often isolated from environmental sources, including soil and water.Legionellaspp. are capable of replicating intracellularly within free-living protozoa, and once this has occurred,Legionellais particularly resistant to disinfectants. Citrus essential oil (EO) vapors are effective antimicrobials against a range of microorganisms, with reductions of 5 log cells ml−1on a variety of surfaces. The aim of this investigation was to assess the efficacy of a citrus EO vapor againstLegionellaspp. in water and in soil systems. Reductions of viable cells ofLegionella pneumophila,Legionella longbeachae,Legionella bozemanii, and an intra-amoebal culture ofLegionella pneumophila(water system only) were assessed in soil and in water after exposure to a citrus EO vapor at concentrations ranging from 3.75 mg/liter air to 15g/liter air. Antimicrobial efficacy via different delivery systems (passive and active sintering of the vapor) was determined in water, and gas chromatography-mass spectrometry (GC-MS) analysis of the antimicrobial components (linalool, citral, and β-pinene) was conducted. There was up to a 5-log cells ml−1reduction inLegionellaspp. in soil after exposure to the citrus EO vapors (15 mg/liter air). The most susceptible strain in water wasL. pneumophila, with a 4-log cells ml−1reduction after 24 h via sintering (15 g/liter air). Sintering the vapor through water increased the presence of the antimicrobial components, with a 61% increase of linalool. Therefore, the appropriate method of delivery of an antimicrobial citrus EO vapor may go some way in controllingLegionellaspp. from environmental sources.

Comparative Study of Acid Etching on Dental Enamel

2018 ◽  
Vol 69 (10) ◽  
pp. 2913-2915
Author(s):  
Daniela Jumanca ◽  
Anamaria Matichescu ◽  
Atena Galuscan ◽  
Laura Cristina Rusu ◽  
Cornelia Muntean
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Experimental Study ◽  
Orthophosphoric Acid ◽  
Dental Enamel ◽  
Acid Etching ◽  
Sealing Material ◽  
Mechanical Bond ◽  
Materials Used ◽  
Scanning Electron

This experimental study aims to analyse the effectiveness of various materials used in demineralisation of dental enamel. This work aims to create a mechanical bond by filling the pegs with sealing material. In order to achieve this goal, five teeth were compared using different concentrations of orthophosphoric acid and exposure times. In this regard, five different tests were performed and the results were analysed using the SEM technique (scanning electron microscopy). These comparative analyses revealed that etching using 35% orthophosphoric acid for one minute and etching using Icon Etch for two minutes were the most effective.

scholarly journals 354. The Epidemiology and Clinical Features of Acanthamoeba Disease in the United States, 1956–2018

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S246-S246
Author(s):  
Kevin O’Laughlin ◽  
Jennifer R Cope ◽  
Zachary A Marsh
Keyword(s):  
United States ◽  
Case Reports ◽  
The United States ◽  
Severe Illness ◽  
People Living With Hiv ◽  
Cutaneous Lesions ◽  
Free Living ◽  
Organ Systems ◽  
Free Living Ameba ◽  
Soil And Water

Abstract Background Acanthamoeba is a free-living ameba found worldwide in soil and water that can cause severe illness. Transmission is thought to be through the skin, eyes, or lungs; Acanthamoeba can cause disseminated infection (Acanthamoeba disease) in addition to the more widely recognized Acanthamoeba keratitis. Infections however are rare, and only case reports or small case series have been published. We review Acanthamoeba disease cases from the Centers for Disease Control and Prevention (CDC) free-living ameba registry to characterize the disease in the United States. Methods CDC maintains a free-living ameba (FLA) registry of laboratory-confirmed Acanthamoeba cases (excluding keratitis) sourced from published case reports, CDC case report forms, and CDC laboratory results. SAS© version 9.4 software was used to calculate descriptive statistics and frequencies. Results We identified 163 cases of Acanthamoeba disease between 1956 and 2018. Of cases with documented outcome, 85% were fatal (105/124). Most (88%) cases were in patients who were immunocompromised (136/155): 66 people living with HIV (of whom 49 were classified as having AIDS); 33 recipients of organ transplantation; 30 people diagnosed with malignancy. The most common manifestation of disease was encephalitis (49%). Other clinical presentations included cutaneous lesions (20%) and rhinosinusitis (6%); 40 cases involved multiple organ systems. Median patient age was 42 years (range 0–83 years). Males accounted for 71% (114/160) of cases. California (29) and Texas (14) had the most case reports; 30 other states reported cases. The source of exposure was unknown in most cases (75%); soil and water were documented in 14 and 17 cases, respectively. Conclusion Acanthamoeba disease in the United States is primarily characterized by encephalitis and cutaneous lesions that affect predominately immunocompromised individuals. Acanthamoeba as a cause of encephalitis in immunocompromised patients should be considered by clinicians, which may lead to earlier diagnosis and treatment. Disclosures All Authors: No reported disclosures

A new rat model of chronic cerebral hypoperfusion associated with arteriovenous malformations

2002 ◽  
Vol 97 (5) ◽  
pp. 1198-1202 ◽  
Author(s):  
Jian Hai ◽  
Meixiu Ding ◽  
Zhilin Guo ◽  
Bingyu Wang
Keyword(s):  
Electron Microscopy ◽  
Animal Model ◽  
Arteriovenous Malformations ◽  
Perfusion Pressure ◽  
Chronic Cerebral Hypoperfusion ◽  
Cerebral Hypoperfusion ◽  
Model Group ◽  
Content Type ◽  
The Right ◽  
Fine Print

Object. A new experimental model of chronic cerebral hypoperfusion was developed to study the effects of systemic arterial shunting and obstruction of the primary vessel that drains intracranial venous blood on cerebral perfusion pressure (CPP), as well as cerebral pathological changes during restoration of normal perfusion pressure. Methods. Twenty-four Sprague—Dawley rats were randomly assigned to either a sham-operated group, an arteriovenous fistula (AVF) group, or a model group (eight rats each). The animal model was readied by creating a fistula through an end-to-side anastomosis between the right distal external jugular vein (EJV) and the ispilateral common carotid artery (CCA), followed by ligation of the left vein draining the transverse sinus and bilateral external carotid arteries. Systemic mean arterial pressure (MAP), draining vein pressure (DVP), and CPP were monitored and compared among the three groups preoperatively, immediately postoperatively, and again 90 days later. Following occlusion of the fistula after a 90-day interval, blood—brain barrier (BBB) disruption and water content in the right cortical tissues of the middle cerebral artery territory were confirmed and also quantified with transmission electron microscopy. Formation of a fistula resulted in significant decreases in MAP and CPP, and a significant increase in DVP in the AVF and model groups. Ninety days later, there were still significant increases in DVP and decreases in CPP in the model group compared with the other groups (p < 0.05). Damage to the BBB and brain edema were noted in animals in the model group during restoration of normal perfusion pressure by occlusion of the fistula. Electron microscopy studies revealed cerebral vasogenic edema and/or hemorrhage in various amounts, which correlated with absent astrocytic foot processes surrounding some cerebral capillaries. Conclusions. The results demonstrated that an end-to-side anastomosis between the distal EJV and CCA can induce a decrease in CPP, whereas a further chronic state of cerebral hypoperfusion may be caused by venous outflow restriction, which is associated with perfusion pressure breakthrough. This animal model conforms to the basic hemodynamic characteristics of human cerebral arteriovenous malformations.

Sterile shunt malfunction

1984 ◽  
Vol 61 (6) ◽  
pp. 1079-1084 ◽  
Author(s):  
David J. Gower ◽  
Jon C. Lewis ◽  
David L. Kelly
Keyword(s):  
Electron Microscopy ◽  
Cellular Response ◽  
Internal Surface ◽  
Giant Cells ◽  
Shunt Malfunction ◽  
Delayed Hypersensitivity ◽  
Silicone Implants ◽  
Content Type ◽  
Joint Implants ◽  
Delayed Hypersensitivity Reaction

✓ Recently, a delayed hypersensitivity reaction to silicone plastics has been demonstrated in some patients with breast and joint implants and subcutaneously injected silicone. In this study, the authors examined the internal surface of shunting systems to evaluate the cellular response to implanted silicone plastic. The distal peritoneal tubing from 20 patients with ventriculoperitoneal shunts was examined with a scanning electron microscope. Twelve tubes were removed because of documented distal shunt malfunction and eight for an elective lengthening procedure. Cultures of all tubing were negative. The catheters removed for malfunction contained a variety of cells: sometimes in clumps, sometimes associated with platelets and densely adherent to the silicone tubing wall. In several shunts, giant cells were seen with multiple foot processes adherent to the internal silicone surface. The internal surface of the tubing of two malfunctioning shunts was embedded in electron microscopy plastic and studied with transmission electron microscopy. The cells proved to be neutrophils with no evidence of silicone granules inside the cell bodies. The shunts removed for elective revision showed only a few cells, and those were never associated with platelets. There was proteinaceous material scattered on the internal surface of the tubing, but the cellular response was markedly different from that in malfunctioning shunts. The authors postulate that the findings in malfunctioning shunt tubing represent a delayed hypersensitivity to silicone similar to that seen with other types of silicone implants.

Experimental study of pin surface precision on lubrication behavior of pin–bush pairs in industrial roller chains

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Ansheng Zhang ◽  
Mingyu Zhang ◽  
Jing Wang ◽  
Jianjun Zhang ◽  
Zhaohua Shang ◽  
...  
Keyword(s):  
Experimental Study ◽  
Design Methodology ◽  
Industrial Applications ◽  
Experimental Basis ◽  
Reciprocating Motion ◽  
Content Type ◽  
Oil Supply ◽  
Machining Errors ◽  
Surface Precision ◽  
Straightness Error

Purpose The purpose of this paper is to study the influence of surface precision on the lubrication state of the roller chain under adequate and rare oil supply conditions, respectively. Design/methodology/approach The straightness error and roughness error of the pin generatrices were measured and the influence of surface precision on the lubrication behavior under steady state and reciprocating motion was studied through optical interference experiments. Findings The lubrication state is strongly influenced by the surface precision of the roller surface both under adequate oil supply and rare oil supply conditions. Originality/value In industrial applications, the machining errors of parts cannot be completely eliminated. Studying the influence of the surface precision on the lubrication behavior of pin–bush pairs can provide the experimental basis for the optimal design of the bush roller chains.

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