scholarly journals Impaired Gamma Interferon Response to Mycobacterium vaccae Antigens in Patients with Cavitary Pulmonary Tuberculosis

2008 ◽  
Vol 15 (9) ◽  
pp. 1485-1488 ◽  
Author(s):  
Elisabeth Rodríguez-Güell ◽  
Gemma Agustí ◽  
Mercè Corominas ◽  
Marina Luquin ◽  
Esther Julián
Keyword(s):  
Cell Wall ◽  
Pulmonary Tuberculosis ◽  
Cytokine Production ◽  
Interleukin 10 ◽  
Gamma Interferon ◽  
Interferon Response ◽  
Tuberculosis Patients ◽  
Mycobacterium Vaccae ◽  
Cavitary Disease ◽  
Specific Antigens

ABSTRACT The ability of tuberculosis patients to recognize Mycobacterium vaccae-specific antigens before starting chemotherapy and according to disease severity was analyzed. We report that the M. vaccae cell wall skeleton fraction triggers more enhanced cytokine production than the whole bacterium. Moreover, a tendency was observed for a lower gamma interferon/interleukin-10 ratio in patients with cavitary disease induced by M. vaccae antigens.

scholarly journals Mice with Pulmonary Tuberculosis Treated with Mycobacterium vaccae Develop Strikingly Enhanced Recall Gamma Interferon Responses to M. vaccae Cell Wall Skeleton

2008 ◽  
Vol 15 (5) ◽  
pp. 893-896 ◽  
Author(s):  
Elisabeth Rodríguez-Güell ◽  
Gemma Agustí ◽  
Mercè Corominas ◽  
Pere-Joan Cardona ◽  
Marina Luquin ◽  
...  
Keyword(s):  
Cell Wall ◽  
Pulmonary Tuberculosis ◽  
Gamma Interferon ◽  
Subcellular Fractions ◽  
Mycobacterium Vaccae ◽  
Immunotherapeutic Agent ◽  
Interferon Responses

ABSTRACT Whole heat-killed Mycobacterium vaccae is used as an immunotherapeutic agent in tuberculosis (TB), but the compound(s) that triggers its immunostimulatory ability is not known. Here, we show that among different subcellular fractions, the cell wall skeleton induced a prominent expression of gamma interferon in splenocytes from both non-TB and TB M. vaccae-treated mice.

scholarly journals DIFFERENCES OF INTERLEUKIN-18 AND INTERLEUKIN-10 LEVELS IN RIFAMPICIN RESISTANT AND RIFAMPICIN SENSITIVE PULMONARY TUBERCULOSIS PATIENTS IN DR. SOETOMO HOSPITAL SURABAYA

2020 ◽  
Vol 8 (2) ◽  
pp. 116
Author(s):  
Audrey Gracelia Riwu ◽  
Jusak Nugaraha ◽  
Yoes Prijatna Dachlan
Keyword(s):  
Pulmonary Tuberculosis ◽  
Inflammatory Cytokine ◽  
Interleukin 10 ◽  
Interleukin 18 ◽  
Research Subjects ◽  
Short Term Treatment ◽  
Tuberculosis Patients ◽  
Pulmonary Tb ◽  
Significant Difference ◽  
The Mean

Rifampicin is an anti-tuberculosis drug which has an efficient antimicrobial effect and the basis of a short-term treatment regimen for tuberculosis (TB) patients. Rifampicin plays an important role against the growth and slow metabolism of Bacilli M. tuberculosis. Resistance to rifampicin causes the duration of tuberculosis treatment to be longer. Interleukin-18 (IL-18) is a pro-inflammatory cytokine which plays a role in controlling the growth of M. tuberculosis through its ability to induce IFN-γ, while Interleukin-10 (IL-10) is an anti-inflammatory cytokine which plays a role in limiting tissue damage due to the inflammatory process and maintain tissue homeostasis. IL-18 and IL-10 has an important role in explaining the different degrees of inflammation in rifampicin resistant (RR) and rifampicin sensitive (RS) pulmonary tuberculosis patients. The purpose of this study is to determine different levels of IL-18 and IL-10 in new TB patients with RR and RS. This study was a retrospective cohort study with a cross-sectional design carried out from August-November 2018 in the TB-DOTS/MDR clinic at Dr. Soetomo Hospital, Surabaya. 50 research subjects were examined and grouped into two groups, namely pulmonary TB with RR (n = 25) and pulmonary TB with RS (n = 25) based on GeneXpert examination and anti-tuberculosis drug therapy ≤ 1 month. IL-18 and IL-10 were measured using the ELISA Method. Differences in IL-18 and IL-10 levels between groups were analyzed using the Mann-Whitney test. The mean level of IL-18 (pg/ml) in RR and RS pulmonary TB patients were 1273.53±749.86 and 787.96 ±589.28 respectively. The mean level of IL-10 (pg/ml) in RR and RS pulmonary TB patients were 125.25±118.32 and 128.81±135.77 repectively. The mean level of IL-18 in RR and RS pulmonary TB patients were found to have a significant difference, while the mean level of IL-10 did not have a significant difference. Keywords: Interleukin-18, Interleukin-10, Tuberculosis, Rifampicin Resistant, Rifampicin Sensitive

scholarly journals Association of Interleukin-10 Cytokine Expression Status with HLA Non-DRB1*02 and Mycobacterium bovisBCG Scar-Negative Status in South Indian Pulmonary Tuberculosis Patients

2001 ◽  
Vol 69 (9) ◽  
pp. 5635-5642 ◽  
Author(s):  
V. Dheenadhayalan ◽  
S. Shanmugalakshmi ◽  
S. Vani ◽  
P. Muthuveeralakshmi ◽  
G. Arivarignan ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
De Novo ◽  
Significant Risk ◽  
Interleukin 10 ◽  
Cytokine Expression ◽  
Interleukin 4 ◽  
Immune Reactivity ◽  
Tuberculosis Patients ◽  
South Indian ◽  
Ifn Γ

ABSTRACT HLA DRB1*02 and its subtypes predispose individuals for a far-advanced sputum-positive pulmonary tuberculosis transcending ethnic boundaries. Mycobacterium bovis BCG does not afford the desired protection against adult pulmonary tuberculosis, and a spectrum of immune reactivity exists in controls and hospital contacts. All of these findings have been identified and demonstrated in areas of endemicity. Skewing of immunity from protective to pathogenic may involve a shift in the Th1-Th2 paradigm. To elaborate these ideas, we studied gamma interferon (IFN-γ), interleukin-4 (IL-4), and IL-10 cytokine expression in 71 adult pulmonary tuberculosis patients and 74 controls from areas of endemicity in south India by 48-h microculture and reverse transcription-PCR. Most of the patients and controls expressed IFN-γ de novo, and in the presence of purified protein derivative (PPD), all of them expressed significantly higher levels of IFN-γ, suggesting a PPD-specific recall memory. HLA DRB1* allele-dependent IFN-γ expression was identified only in controls, suggesting a skewing of the immune response in patients. In contrast to the case for IFN-γ, only some patients and controls expressed IL-4 or IL-10 (Th2 profile); thus, the Th1 profile was identifiable only by a nonexpression of IL-4 or IL-10 in this area of endemicity. The Th2 profile was associated with HLA non-DRB1*02 and BCG scar-negative status in patients, attributing a significant risk (odds ratio = 2.074; 95% confidence interval = 0.612 to 7.07). It is possible that Mycobacterium tuberculosis (PPD)-specific IL-10 is expressed preemptively in unvaccinated (BCG scar-negative) individuals with a non-DR2 genetic background by chronic exposure in this area of endemicity and leads to pulmonary tuberculosis of adults.

scholarly journals Extended Culture Enhances Sensitivity of a Gamma Interferon Assay for Latent Mycobacterium tuberculosis Infection

2007 ◽  
Vol 14 (6) ◽  
pp. 796-798 ◽  
Author(s):  
Ana Cehovin ◽  
Jacqueline M. Cliff ◽  
Philip C. Hill ◽  
Roger H. Brookes ◽  
Hazel M. Dockrell
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Latent Tuberculosis ◽  
Gamma Interferon ◽  
Mycobacterium Tuberculosis Infection ◽  
Blood Samples ◽  
Tuberculosis Patients ◽  
Household Contacts ◽  
Prolonged Culture ◽  
Release Assay ◽  
Specific Antigens

ABSTRACT To test the hypothesis that prolonged culture would enhance the sensitivity of latent tuberculosis detection by a gamma interferon release assay, blood samples from 33 household contacts of Gambian tuberculosis patients were stimulated with Mycobacterium tuberculosis-specific antigens. After 24 h of culture, 66% were positive, compared to 93% after 6 days of culture.

scholarly journals Monocyte-Derived Interleukin-10 Depresses the Bordetella pertussis- Specific Gamma Interferon Response in Vaccinated Infants

2009 ◽  
Vol 16 (12) ◽  
pp. 1816-1821 ◽  
Author(s):  
V. Dirix ◽  
V. Verscheure ◽  
T. Goetghebuer ◽  
M. Hainaut ◽  
A. S. Debrie ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Bordetella Pertussis ◽  
Mononuclear Cells ◽  
Gene Promoter ◽  
Interleukin 10 ◽  
Gamma Interferon ◽  
Interferon Response ◽  
Ifn Γ

ABSTRACT Antigen-specific gamma interferon (IFN-γ) has been demonstrated to participate in protection against Bordetella pertussis infection. Circulating mononuclear cells from B. pertussis-infected and from pertussis-vaccinated infants secrete high amounts of IFN-γ after in vitro stimulation by B. pertussis antigens, but with a large variation in the secreted IFN-γ levels between individuals. We show here that the inhibition of the specific IFN-γ response can be at least partially attributed to IL-10 secretion by monocytes. This IL-10 secretion was not associated with polymorphisms at positions −1082, −819, and −592 of the IL-10 gene promoter, suggesting that other genetic or environmental factors affect IL-10 expression and secretion.

CD4+ T Cell Clones Producing both Interferon-γ and Interleukin-10 Predominate in Bronchoalveolar Lavages of Active Pulmonary Tuberculosis Patients

1999 ◽  
Vol 92 (3) ◽  
pp. 224-234 ◽  
Author(s):  
Franca Gerosa ◽  
Carla Nisii ◽  
Stefano Righetti ◽  
Rocco Micciolo ◽  
Martina Marchesini ◽  
...  
Keyword(s):  
T Cell ◽  
Pulmonary Tuberculosis ◽  
Interleukin 10 ◽  
Interferon Γ ◽  
Cd4 T Cell ◽  
T Cell Clones ◽  
Tuberculosis Patients ◽  
Active Pulmonary Tuberculosis ◽  
Cell Clones

Cytokine production and mRNA expression in pulmonary tuberculosis patients and their household contacts of younger age group (15–25years)

2016 ◽  
Vol 432 ◽  
pp. 65-71
Author(s):  
Lavanya Joshi ◽  
Meenakshi Ponnana ◽  
Ramya Sivangala ◽  
Lakshmi Kiran Chelluri ◽  
Pratibha Nallari ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Mrna Expression ◽  
Cytokine Production ◽  
Age Group ◽  
Tuberculosis Patients ◽  
Household Contacts ◽  
Younger Age ◽  
Group 15

Detection of Mycobacterium tuberculosis in smear negative sputum by PCR

2012 ◽  
Vol 6 (2) ◽  
pp. 2-6 ◽  
Author(s):  
Mohammad Jobayer ◽  
SM Shamsuzzaman ◽  
Kazi Zulfiquer Mamun
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Accurate Method ◽  
Major Health Problem ◽  
Major Health ◽  
Tuberculosis Patients ◽  
Diagnostic Potential ◽  
Total Death ◽  
Smear Negative ◽  
Negative Sputum

Pulmonary tuberculosis is a major health problem in Bangladesh that is responsible for about 7% of total death in a year. This study was conducted to isolate and identify Mycobacterium tuberculosis from sputum and to evaluate the efficacy of PCR as a modern diagnostic tool, for diagnosis of pulmonary tuberculosis, especially in the smear negative cases. One hundred and fifty suspected pulmonary TB patients (male/ female: 97/53) were included in this study. Single morning sputum was collected from each patient and diagnostic potential of PCR was compared with staining and culture. Twenty five (16.7%) sputum were positive by ZN stained smear. Among 125 smear negative samples, 13 (10.4%) yielded growth in culture in LJ media and 21 (16.8%) samples were positive by PCR. The sensitivity and specificity of PCR in smear negative cases was 100% and 92.9% respectively. Mean detection time in PCR was 24 hours. PCR detected M. tuberculosis in 21 smear negative and 9 culture negative samples. For diagnosis of tuberculosis in smear negative cases, PCR directly from sputum was a very sensitive and accurate method. In conclusion, PCR may be done, especially in clinically suspected pulmonary tuberculosis patients who remain negative by conventional methods.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19368 Bangladesh J Med Microbiol 2012; 06(02): 2-6

Kualitas Hidup Pasien Tuberkulosis Paru yang Mengalami Stigma Diri (Quality of Life Pulmonary Tuberculosis Patients With Self Stigma))

2019 ◽  
Vol 3 (2) ◽  
pp. 17-27
Author(s):  
Yunita Sari
Keyword(s):  
Quality Of Life ◽  
Pulmonary Tuberculosis ◽  
Descriptive Study ◽  
Negative Influence ◽  
Questionnaire Data ◽  
Average Quality ◽  
Tuberculosis Patients ◽  
Pulmonary Tb ◽  
Life Score

Pulmonary tuberculosis (TB) is a chronic disease that can bring about the sufferer's self-stigma and also affect his quality of life. A number of studies report that living with TB has a negative influence on the quality of life of sufferers even with or without self-stigma. The purpose of this study was to identify the quality of life of TB patients who experienced self-stigma. This research is a descriptive study, sample were 31 pulmonary TB patients. Data was collected using a questionnaire. Data analyzed by using frequency distribution and percentage. The researcher first screened TB patients who experienced self-stigma. The results showed that 25 people (80.64%) respondents experienced mild self-stigma. A total of 9 respondents (36%) had a quality of life score in the good category and as many as 16 respondents (64%) had enough category with an average quality of life score is 56.57. While respondents who had moderate self-stigma were 6 people (19.36%) with a good quality of life score was 1 person (16.67%) and enough category quality of life score were 5 people (83.33%) with an average quality of life score is 49.92.

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