scholarly journals Evaluation of an Indirect Immunofluorescence Assay for Strongyloidiasis as a Tool for Diagnosis and Follow-Up

2006 ◽  
Vol 14 (2) ◽  
pp. 129-133 ◽  
Author(s):  
Marina Boscolo ◽  
Maria Gobbo ◽  
William Mantovani ◽  
Monica Degani ◽  
Mariella Anselmi ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Antibody Titer ◽  
Indirect Immunofluorescence ◽  
Serum Antibody ◽  
Antibody Test ◽  
Strongyloides Stercoralis ◽  
Negative Control ◽  
Response To Treatment ◽  
Diagnostic Threshold ◽  
Antibody Titers

ABSTRACT The diagnostic accuracy of an indirect immunofluorescence antibody test (IFAT) for Strongyloides stercoralis at different serum antibody titers was evaluated. To assess diagnostic sensitivity, sera from 156 patients with known strongyloidiasis were collected. Negative control sera were obtained from a composite group of 427 subjects (blood donors and hospitalized patients). With an area under the receiver-operating characteristic plot of 0.98, the IFAT showed a high level of diagnostic accuracy for strongyloidiasis. An antibody titer of ≥1:20, with 97% sensitivity and 98% specificity, was identified as the diagnostic threshold with the best overall performance. Cross-reactions were evaluated with 41 additional samples from patients with other known helminth infections, and the IFAT detected low-titer positivity in only one subject with filariasis. A positive IFAT result at an antibody dilution of ≥1:80 was virtually 100% specific, with 71% sensitivity. To test the usefulness of the IFAT as a monitoring tool, the changes in specific-antibody titers after treatment in a group of 155 patients were evaluated. Seroreversion or a decrease in antibody titer of twofold or more was observed in 60% of the patients. Response to treatment was directly correlated to the initial antibody titer, and a baseline titer of ≥1:80 was identified as the best predictor of response. In conclusion, a positive IFAT result at an antibody dilution of ≥1:20 is the optimal cutoff for screening. A titer of ≥1:80, with virtually no false-positive result, is a reliable cutoff for a serological assessment of treatment efficacy and for inclusion in clinical trials.

scholarly journals Enzyme-linked immunospecific antibody test for detecting antibody to Klebsiella

1978 ◽  
Vol 8 (6) ◽  
pp. 704-707
Author(s):  
J P Rissing ◽  
T B Buxton ◽  
W L Moore ◽  
T Ozawa ◽  
W L Moore
Keyword(s):  
Antibody Titer ◽  
Indirect Immunofluorescence ◽  
Infected Patient ◽  
Capsular Polysaccharide ◽  
High Titer ◽  
Antibody Test ◽  
Standard Test ◽  
Microtiter Plates

The enzyme-linked immunospecific antibody test was performed in standard test tubes and microtiter plates to meausre high-titer antibody against Klebsiella capsular polysaccharide. Initial studies were conducted with rabbit sera; other studies were conducted with the serum of a patient infected with type 9 Klebsiella. Both immunized rabbits and an infected patient disclosed high titers of anticapsular antibody. Control sera from other immunized rabbits and other infected humans failed to show this substantial antibody titer against type 9 Klebsiella. Comparisons between counterimmunoelectrophoresis and indirect immunofluorescence disclosed that the sensitivity of the enzyme-linked immunospecific antibody test for anti-Klebsiella antibody ranged between 400 and 10,000 times that of these tests.

scholarly journals Determination of Sero-Prevalence of SARS-CoV-2 antibody among immunized individuals with Covid-19 vaccine in a tertiary center, Nepal

2022 ◽  
pp. 62-65
Author(s):  
Gopal Lama ◽  
Lilee Shrestha ◽  
Nabin Karmacharya ◽  
Rekha Manandhar ◽  
Runa Jha
Keyword(s):  
Antibody Titer ◽  
Serum Antibody ◽  
Antibody Test ◽  
Cross Sectional Study ◽  
Antibody Testing ◽  
Cross Sectional ◽  
Robust Immune Response ◽  
Tertiary Center

Background: Coronavirus Disease 2019 (covid-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). People who are infected with SARS-CoV-2 or are vaccinated with covid-19 vaccines are supposed to develop immunoglobulins and these immune responses in human body will determine the efficacy of the vaccines as well as help to discover new therapeutic options. Methods: A cross-sectional study conducted between April to June, 2021, assessing serum antibody titer from participants who had taken the first dose of covishieldTM vaccine (naïve as well as prior covid-19 infected individuals). Antibody testing was carried out with Roche Elecsys Anti-SARS-CoV-2 S electrochemiluminescence immunoassay on Roche Cobas e 601 module. Twenty-eight of these participants had follow up repeat antibody test after second dose of vaccine. Results: A total of 122 participants with the first dose of CovishieldTM vaccine were all tested seropositive, antibody titer ranging from minimum of 2.95 U/mL to maximum 2500 U/mL. Average antibody titer was 308.9 U/mL for naive cohort and 1604 U/mL for prior covid-19 infection. In twenty-eight participants who had antibody titer measured after 1 month of second dose, average titer was 1459.7 U/mL for naïve cohort and 1803.4 U/mL for prior covid-19 infected individuals, which was statistically significant compared to antibody response after the first dose. Conclusions: Antibody responses against SARS-CoV-2 following immunization was 100%, with significant development after second dose in naïve population while robust immune response was present after first dose in prior SARS-CoV-2 infected individuals.

Novel malignant-mesothelioma-associated antigens (Gene-X and THBS-2) in the diagnosis of malignant pleural mesothelioma (MPM).

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. 10585-10585
Author(s):  
Fumihiro Tanaka ◽  
Yoshiki Shigematsu ◽  
Takeshi Hanagiri ◽  
Hidetaka Uramoto ◽  
Tomoko So ◽  
...  
Keyword(s):  
Antibody Titer ◽  
Malignant Pleural Mesothelioma ◽  
Early Stage ◽  
Asbestos Exposure ◽  
Serum Antibody ◽  
Diagnostic Value ◽  
Pleural Mesothelioma ◽  
Roc Curve Analysis ◽  
Antibody Titers ◽  
Serum Antibody Titer

10585 Background: Malignant pleural mesothelioma (MPM) is a highly aggressive malignant tumor of the pleura associated with asbestos exposure, and its diagnosis is usually difficult at early stage. We identified novel mesothelioma-related antigens, Gene-X and thrombospondin-2 (THBS-2), recognized by tumor-infiltrating B cells (Cancer Sci 2009), but the clinical significance in the diagnosis of MPM remains unclear. Methods: A total of 120 patients, who presented with a suspicion of MPM and received pleural biopsy, were reviewed; 97 patients were finally diagnosed with MPM and 27 were with non-malignant diseases (NM). The antibody-titers against Gene-X and THBS-2 in the sera were measured by ELISA method. Results: The serum antibody-titer against THBS-2 was significantly higher in MPM patients than in NM (P<0.01), but there was no difference in the serum antibody-titer against Gene-X (Table). The receiver operating characteristic (ROC) curve analysis showed a significant diagnostic value of serum antibody-titer against THBS-2 with the area-under curve of 0.886 (95% CI, 0.797 - 0.975; P<0.001) in discrimination of MPM from NM diseases; the sensitivity and specificity, when the cut-off value was 0.08, were 72.2% and 95.5%, respectively. Conclusions: The serum antibody-titer against THBS-2 can be a useful non-invasive marker in the diagnosis of MPM. [Table: see text]

scholarly journals Post Vaccination Titers of Serum Anti-Diphtheria in Indonesian Young and Middle-Aged Adults

2021 ◽  
Author(s):  
Gatot Soegiarto ◽  
Diksi Novritasari ◽  
Ari Baskoro ◽  
Deasy Fetarayani ◽  
Wita Kartika Nurani
Keyword(s):  
Antibody Titer ◽  
Serum Antibody ◽  
Age Groups ◽  
Middle Aged ◽  
Adult Age ◽  
Significant Difference ◽  
Antibody Titers ◽  
The Difference ◽  
Middle Aged Adult ◽  
Middle Aged Adults

Objective: This study aimed to evaluate the antibody responses in two adult age groups after diphtheria vaccination. Study Design: An observational analytic study was carried out to determine the difference in serum titer of anti-diphtheria antibody. Methods: Serum antibody titers were measured just before and 3 months after injection of Diphtheria toxoid vaccine. Vaccine was given to two adult age groups of health care personnel in hospital: the young (&lt; 40 years) and the middle-aged (≥ 40 years). Data were analyzed using the Mann-Whitney test (p &lt; 0.05). Results: Significant increase in serum anti-diphtheria antibody titers were recorded after vaccination in both age group (p &lt; 0.001 in young adult and p = 0.001 in middle-aged adult, respectively). There were no substantial differences between the two groups in terms of antibody titer before vaccination (p = 0.741), 3 months after vaccination (p = 0.317) and in the increase of antibody titer (p = 0.479). Conclusions: This study showed that there was no significant difference in the increase of anti-diphtheria antibody titers between the two age groups, proving that both young and middle-aged adults had an equal immune response to a given diphtheria vaccine.

scholarly journals Gynura procumbens (Lour.) Merr. Leaves Against Mice’s Antibody Titer

2019 ◽  
Vol 8 (2) ◽  
pp. 13-19
Author(s):  
Rahimatul Uthia ◽  
Elin Resti Ananda ◽  
Ifora Ifora
Keyword(s):  
Body Weight ◽  
Antibody Titer ◽  
White Male ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Defense System ◽  
Leaf Extracts ◽  
Antibody Titers

The body's defense system can be enhanced by certain compounds that are immunostimulant. An antibody titer is a measure of the body's level of immunity to a disease. Titers are usually expressed in terms of the number of times they can thin the blood until they cannot find any more antibodies. This study aims to determine the activity of the extracts of the leaves of Gynura Procumbens (Lour.) Merr. against antibody titers, the number, and percentage of leukocyte cells of white male mice. 25 mice were divided into 5 groups consisting of negative control, positive control, group dose 75 mg/kg body weight, 150 mg/kg body weight, 300 mg/kg body weight. The extract is given for 7 days orally. The results showed that the administration of leaf extracts can increase antibody titers and increase with increasing doses, as well as increasing the total number of leukocytes and the percentage of leukocyte cells significantly (P <0.05).

scholarly journals COMPARISON OF A RAPID ENZYME-LINKED IMMUNOSORBANT ASSAY TEST WITH AN INDIRECT IMMUNOFLUORESCENT ANTIBODY TEST IN DIAGNOSING EHRLICHIA AND ANAPLASMA INFECTIONS IN DOGS

2019 ◽  
Vol 17 (4) ◽  
pp. 346-352
Author(s):  
Kr. Gospodinova ◽  
G. Zhelev ◽  
V. Petrov
Keyword(s):  
Serum Antibody ◽  
Rapid Test ◽  
Antibody Test ◽  
Diagnostic Value ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Immunofluorescent Antibody ◽  
Antibody Titers ◽  
Indirect Immunofluorescent ◽  
Immunofluorescent Antibody Test

PURPOSE: The objective of this study is to compare the diagnostic value of commercial enzyme-linked immunosorbent assay (ELISA) with indirect immunofluorescent antibody test (IFA) in detecting immunoglobulin-G (IgG) antibodies to Ehrlichia canis and Anaplasma phagocytophilum. METHODS: Seventy-four serum samples, obtained from dogs believed to be naturally infected with E. canis or A. phagocytophilum, were analyzed. RESULTS: By ELISA, 48 (64.9%) samples were found positive for IgG to E. canis, 10 (13.5%) to A. phagocytophilum, 12 (16.2%) to both E. canis and A. phagocytophilum, and in 4 (5.4%) samples no presence of antibodies was detected. The number of serologically positive dogs for IgG was 44 (59.5%) to E. canis, 10 (13.5%) to A. phagocytophilum, 16 (21.6%) to both E. canis and A. phagocytophilum, and 4 (5.4%) were determined negative by means of IFA. In most samples the antibody titer did not exceed 1:80 but in 5 it reached a level of 1:320, and in other 4 of even above 1:640. CONCLUSIONS: This study shows that IFA assay is more sensitive than commercial ELISA rapid test when serum antibody titers are low.

scholarly journals Diagnostic Accuracy of the E-Plate Serum Antibody Test Kit in Detecting Helicobacter pylori Infection Among Japanese Children

2014 ◽  
Vol 24 (1) ◽  
pp. 47-51 ◽  
Author(s):  
Junko Ueda ◽  
Masumi Okuda ◽  
Takeshi Nishiyama ◽  
Yingsong Lin ◽  
Yoshihiro Fukuda ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Diagnostic Accuracy ◽  
Serum Antibody ◽  
Antibody Test ◽  
Helicobacter Pylori Infection ◽  
Japanese Children ◽  
Test Kit

scholarly journals DETECTION OF TOXOPLASMOSIS IN CATS AND SHEEP

2021 ◽  
Vol 26 (1) ◽  
pp. 159-164
Author(s):  
Osama J. Kallo
Keyword(s):  
Antibody Titer ◽  
Antibody Test ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Recurrent Abortion ◽  
Serum Samples ◽  
Serological Tests ◽  
Fecal Samples ◽  
Antibody Titers ◽  
Latex Test

Thirty-three fecal samples from cats were examined for the presence of Toxoplasma oocysts, and another 33 serum samples from these cats were subjected for Latex agglutination test & indirect immunofluorecent antibody test. Also 80 serum samples from ewes were subjected to the same serological tests. The study indicated that the prevalence of Toxoplasma oocysts in cats was 27.3%. Higher rates of antibody titer (68%) were observed in cats tested with latex test. Infection in young cats was higher than in adults. Sixty percentage of ewes were sero-positive with Latex test, but only 35% were sero-positive with IFAT, higher prevalence of antibody titers was observed in sheep from the three locations of Iraq. Ewes that had recurrent abortion showed higher prevalence in both tests than non aborted ewes.

scholarly journals Serodiagnosis and Bacterial Genome of Helicobacter pylori Infection

Toxins ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 467
Author(s):  
Aina Ichihara ◽  
Hinako Ojima ◽  
Kazuyoshi Gotoh ◽  
Osamu Matsushita ◽  
Susumu Take ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antibody Titer ◽  
Bacterial Genome ◽  
Serum Antibody ◽  
Gene Mutations ◽  
Bacterial Genomes ◽  
Western Blots ◽  
A Genome ◽  
Vaca Gene ◽  
H Pylori

The infection caused by Helicobacter pylori is associated with several diseases, including gastric cancer. Several methods for the diagnosis of H. pylori infection exist, including endoscopy, the urea breath test, and the fecal antigen test, which is the serum antibody titer test that is often used since it is a simple and highly sensitive test. In this context, this study aims to find the association between different antibody reactivities and the organization of bacterial genomes. Next-generation sequences were performed to determine the genome sequences of four strains of antigens with different reactivity. The search was performed on the common genes, with the homology analysis conducted using a genome ring and dot plot analysis. The two antigens of the highly reactive strains showed a high gene homology, and Western blots for CagA and VacA also showed high expression levels of proteins. In the poorly responsive antigen strains, it was found that the inversion occurred around the vacA gene in the genome. The structure of bacterial genomes might contribute to the poor reactivity exhibited by the antibodies of patients. In the future, an accurate serodiagnosis could be performed by using a strain with few gene mutations of the antigen used for the antibody titer test of H. pylori.

scholarly journals Evaluation of diagnostic accuracy of developed rapid SARS-COV-2 IgG antibody test kit using novel diluent system

VirusDisease ◽  
2021 ◽  
Author(s):  
Vani Maya ◽  
Jyothi Embekkat Kaviyil ◽  
Dinoop Koral Ponnambath ◽  
Renjith P. Nair ◽  
Anugya Bhatt ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Antibody Test ◽  
Igg Antibody ◽  
Test Kit
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