scholarly journals Gynura procumbens (Lour.) Merr. Leaves Against Mice’s Antibody Titer

2019 ◽  
Vol 8 (2) ◽  
pp. 13-19
Author(s):  
Rahimatul Uthia ◽  
Elin Resti Ananda ◽  
Ifora Ifora
Keyword(s):  
Body Weight ◽  
Antibody Titer ◽  
White Male ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Defense System ◽  
Leaf Extracts ◽  
Antibody Titers

The body's defense system can be enhanced by certain compounds that are immunostimulant. An antibody titer is a measure of the body's level of immunity to a disease. Titers are usually expressed in terms of the number of times they can thin the blood until they cannot find any more antibodies. This study aims to determine the activity of the extracts of the leaves of Gynura Procumbens (Lour.) Merr. against antibody titers, the number, and percentage of leukocyte cells of white male mice. 25 mice were divided into 5 groups consisting of negative control, positive control, group dose 75 mg/kg body weight, 150 mg/kg body weight, 300 mg/kg body weight. The extract is given for 7 days orally. The results showed that the administration of leaf extracts can increase antibody titers and increase with increasing doses, as well as increasing the total number of leukocytes and the percentage of leukocyte cells significantly (P <0.05).

scholarly journals Dietary supplementation of Neem (Azadirachta indica) leaf extracts improved growth performance and reduced production cost in broilers

2020 ◽  
Vol 13 (6) ◽  
pp. 1050-1055
Author(s):  
Torun Kumar Paul ◽  
Md. Mehedi Hasan ◽  
Md. Anowarul Haque ◽  
Sudipta Talukder ◽  
Yousuf Ali Sarker ◽  
...  
Keyword(s):  
Body Weight ◽  
Azadirachta Indica ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Broiler Chicks ◽  
Growth Promoter ◽  
Leaf Extracts ◽  
Neem Leaves

Background and Aim: Global trend to remove the antibiotic growth promoter (AGP) from animals contributes to the exploration of successful measures to sustain production and reduces the intestinal diseases in the post-AGP era. Plant extracts, therefore, have been used to improve performance and intestinal health. Here, we conducted a study to evaluate the effects of neem (Azadirachta indica) leaf extracts (NLE) as alternatives to AGPs in broiler chickens. Materials and Methods: Sixty day-old broiler chicks were assigned to 12-floor pens, each stocked with five birds and divided into three treatment groups of four pens per treatment. The groups were: Negative control, basal diet without additives; positive control, basal diet with antibiotics and vitamins; and NLE treated group, basal diet supplemented with 0.1% aqua extract of neem leaves. Results: Overall feed intake was significantly (p≤0.05) highest in the positive control. Higher body weight, higher dressing percentage, and lower feed conversion ratio were observed in birds treated with NLE compared to the negative control group (p≤0.05) but not the positive control group (p>0.05). There was no significant variation in hematology between different groups. Furthermore, the economic evaluation indicated that the NLE treatment was found cheaper than control and antibiotic treatment in cost-benefit analysis. Conclusion: We suggest NLE might be a cheaper alternative to antibiotics in broiler production as indicated by improved body weight and feed efficiency.

scholarly journals UJI EFEK ANTIPIRETIK EKSTRAK ETANOL DAUN MIANA(COLEUS SCUTELLARIOIDES [L]) BENTH PADA TIKUS PUTIH JANTAN GALUR WISTAR (RATTUS NORVEGICUS)

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 55
Author(s):  
Sri Gentari Benjamin ◽  
Adithya Yudistira ◽  
Henki Rotinsulu
Keyword(s):  
White Male ◽  
Data Retrieval ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Leaf Extracts ◽  
Antipyretic Effect ◽  
Hb Vaccine

ABSTRACTThis study aims to determine the antipyretic effect of miana leaves extract (Coleus scutellariodes L Bent) in white male rats strain wistar. The subjects of this study were 15 white male rats, which were divided into 5 groups, namely the negative control group (1% CMC), the positive control group (Paracetamol), and the treatment group (miana leaf extracts). This type of research is a laboratory experiment, which carried out on the test animals based on a Completely Randomized Design (CRD) by testing the antipyretic effect carried out by administering the DPT-Hb vaccine. The observed response was in the form of data retrieval related to rat temperature after 2 hours of DPT-hb vaccine administration. Body temperature measurements were carried out before administering the vaccine, then successively at the 30th, 60th, 90th, and 120th minutes, respectively. The results showed miana leaf extracts at a dose of 25 mg, 50 mg and 75 mg had an antiperetic effect in white male rats but the dose 75mg provides the most effective antipyretic effect. Thus it can be concluded that the ethanol extracts of miana leaves has an antipyretic effect in white male rats, which were induced orally.  Keywords: Antipyretics, Miana Leaves, Male White Rat. ABSTRAK Penelitian ini bertujuan untuk mengetahui Efek Antipiretik Ekstrak daun Miana (Coleus scutellariodes [L] Bent) pada Tikus Putih Jantan Galur Wistar. Subjek penelitian ini ialah 15 ekor tikus putih jantan yang dibagi menjadi 5 kelompok yaitu kelompok kontrol negatif (CMC 1%), kelompok kontrol positif (Parasetamol), dan kelompok perlakuan (ektrak daun miana).Jenis penelitian ini ialah eksperimental laboratorium yang akan dilakukan pada hewan uji berdasarkan Rancangan Acak Lengkap (RAL) dengan pengujian efek antipiretik dilakukan dengan cara memberikan vaksin DPT-Hb. Respon yang diamati yaitu berupa pengambilan data terkait suhu tikus setelah 2 jam pemberian vaksin DPT-hb. Pengukuran suhu tubuh dilakukan sebelum pemberian Vaksin, kemudian berturut-turut pada menit ke-30, 60, 90, dan 120. Hasil penelitian menunjukan ekstrak daun Miana dengan dosis 25 mg, 50 mg dan 75 mg memiliki efek antiperetik pada tikus putih jantan namun dosis 75 mg memberikan  efek antipiretik yang paling efektif. Dengan demikian dapat disimpulkan bahwa ekstrak daun miana memiliki efek antipiretik pada tikus putih jantan yang di induksi secara oral.  Kata kunci : Antipiretik, Daun Miana, Tikus Putih Jantan

Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Verawaty Verawaty ◽  
Dhea Claudia Novel
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Glucose Levels ◽  
The Third ◽  
Blood Glucose Levels

<p>Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.</p><p><em>Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.</em></p>

scholarly journals In vivo antidiarrheal study of ethanolic extracts of Mikania cordata and Litsea monopetala leaves

2015 ◽  
Vol 10 (3) ◽  
pp. 562 ◽  
Author(s):  
Fatema Nasrin ◽  
Md. Lukman Hakim
Keyword(s):  
Body Weight ◽  
Castor Oil ◽  
Leaf Extract ◽  
Positive Control ◽  
Control Group ◽  
P Value ◽  
Leaf Extracts ◽  
Ethanolic Extracts ◽  
Antidiarrheal Activity

<p class="Abstract">In this study the antidiarrheal activity of ethanolic extracts of the leaves of <em>Mikania cordata</em> and <em>Litsea monopetala</em> was evaluated. Diarrhea was induced in mice by oral administration of castor oil (0.5 mL) 30 min after the administration of the extracts. During a 4 hour study the number of diarrheal feces and percentage inhibition of the extracts (200 and 400 mg/kg body weight) was determined. Loperamide (3 mg/kg body weight) served as standard and belonged to the positive control group. The extracts exhibited potent antidiarrheal activity as well as achieved statistically significant p value (p&lt;0.01 and p&lt;0.05) compared to control group. Among the extracts the highest percentage inhibition of defecation (60%) was recorded for leaf extract (400 mg/kg body weight) of <em>L. monopetala</em>. So, the study corroborates the significant antidiarrheal activity of <em>M. cordata</em> and <em>L. monopetala</em> leaf extracts and raises the demand of further sophisticated investigation.</p><p> </p>

scholarly journals Application of Scaevola taccada (Gaertn.) Roxb. Reduce Pro-inflammatory Cytokines Interleukin-1β in Sprague Dawley Mice Suffering from Mastitis

2020 ◽  
Vol 8 (A) ◽  
pp. 423-427
Author(s):  
Prihantono Prihantono ◽  
Salman Ardi Syamsu ◽  
Nilam Smaradhania ◽  
Mardiana Ahmad ◽  
Nurul Aini Siagian ◽  
...  
Keyword(s):  
Body Weight ◽  
Positive Control ◽  
Mean Value ◽  
Negative Control ◽  
Interleukin 1Β ◽  
Sprague Dawley ◽  
Leaf Extracts ◽  
Group Iii ◽  
Group I ◽  
The Mean

BACKGROUND: Mastitis is an inflammation of the breast tissue, usually caused by bacteria. Mastitis stimulates pro-inflammatory cytokines. The cytokine interleukin-1β (IL-1β) is a crucial mediator of the inflammatory response. This cytokine has adverse effects of hosting immunity that mediates resistance to pathogens and also exacerbates damage during chronic disease and acute tissue injury. Scaevola taccada (Gaertn.) Roxb. has been used as an ethnomedicine for healing sores in several provinces in Indonesia. AIM: This study aimed to assess the profile of pro-inflammatory cytokine IL-1β through the treatment effect of leaf extracts of S. taccada (Gaertn.) Roxb. as adjuvant for healing mastitis. METHODS: This study was a true control group experiment using the pre-test-post-test control design aimed to measure the effect of hydroalcoholic compounds in leaf extracts of S. taccada on the systemic pro-inflammatory activity of interleukin-1β (IL-1β). The treated animals were 18 mice of Sprague Dawley strain induced by Staphylococcus aureus. These treated mice were divided into three groups in which each group consisted of six mice. The mice in the Group I (negative control) were given 1 ml aquabides/250 g body weight, those in the Group II (positive control) were delivered with 9.6 mg/ml amoxicillin/250 g body weight, and those in the Group III (experimental) were given 9.6 mg amoxicillin/250 g body weight + 400 mg/ml leaf extracts of S. taccada/g body weight for 5 days, respectively. Pathological examinations were carried out from the inflamed tissues to prove the healing process of the treated mice. IL-1Β levels were determined using the enzyme-linked immunosorbent assay method. Data were analyzed using ANOVA and post hoc tests. RESULTS: There were statistically significant differences of IL-1β levels after the administration of leaf extracts of S. taccada among all the treated mice groups at p < 0.05. The Group III had the lowest IL-1β level with the mean value ± 1.45 pg/ml compared to the IL-1β level in the Group II (positive control) with the mean value ± 3.82 pg/ml and the IL-1β level in the Group I (negative control) with the with mean value ± 5.22 pg/ml. The pathological analysis of breast tissues of the treated mice proved that leaf extracts of S. taccada (Gaertn Roxb.) could reduce damaged tissues, cellular infiltration, and subcutaneous edema induced by this pathogenic microorganism. CONCLUSION: Leaf extracts of S. taccada had a significant function as adjuvant for healing mastitis by reducing pro-inflammatory cytokine IL-1β.

scholarly journals Ameliorative Effect of Ethanolic Extract of Petroselinum Crispum Against Gentamicin-Induced Nephrotoxicity in Rabbits Male

2020 ◽  
Vol 13 (2) ◽  
Author(s):  
Sarah Hussain ◽  
Mariam Kadhem
Keyword(s):  
Body Weight ◽  
Clinical Signs ◽  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Positive Control Group ◽  
Control Group ◽  
Petroselinum Crispum

The experiment was conducted to investigate the protective effect of Petroselinum crispum leave extracted against gentamicin-induced nephrotoxicity in male rabbits by studying the body weight, clinical signs, haematological and biochemical parameters, gross lesion and histopathological changes. Twenty four rabbits male were used and divided into 4 groups. Group 1: rabbits served as a negative control, received distilled water 1 ml(orally). Group 2: rabbits served as a positive control group, received gentamicin at a dose of 80 mg/kg/day intramuscular for 15 days. Group 3: rabbits received gentamicin at a dose of 80 mg/kg/day then after one hour treated with ethanolic extract of Petroselinum crispum at dose 125 mg/kg orally for 15 days. Group 4: rabbits received gentamicin at a dose of 80 mg/kg/day then after one hour treated with ethanolic extract of Petroselinum crispum at dose 250 mg/kg orally for 15 days.The results of the gentamicin treated group( positive control group) showed clinical signs such as loss of body weight, loss of appetite and rough hair with hematuria. The body weight a significantly declined (p≤ 0.05) compared other groups. There was a significant decrease (p≤ 0.05) in WBC count, lymphocyte, GSH, SOD, CAT, and GPX levels, while it recorded a significant increase (p≤0.05) in weights of the kidneys, neutrophils, creatinine, urea, and MDA. Histological studies showed several kidney pathological changes such as pale colour, enlargement in size and weight and easy from detaching as opposed to negative control group. On the other hand, the group treated with ethanolic extractof Petroselinum crispum at dose 125 mg/kg induced improved of parameters as recorded significant increased(P ≤ 0.05) in body weight, WBC count, lymphocyte, GSH, SOD, CAT, and GPX, while significant decreased (P ≤ 0.05) in weights of the kidneys, neutrophils, creatinine, urea, and MDA compared with the positive control group whereas rabbits treated with ethanolic extract of Petroselinum crispum at dose 250 mg/kg restored the parameters and histological changes of the kidney to near normal status compared with the negative control group. These results showed that the dose-detected Petroselinum crispum extract (250mg / kg) acts as potential curative effect against gentamicin-induced nephrotoxicity in male rabbits.

scholarly journals Immunomodulator Effect of Lemongrass Extract (Cymbopogon nardus L.) to Increase Immune Cells as a Precaution Against SARS-CoV-2

2021 ◽  
Vol 4 (2) ◽  
pp. 73
Author(s):  
Putri Ayu Ika Setiyowati ◽  
Rofiatun Solekha ◽  
Sri Bintang Sahara Mahaputra Kusuma Negara ◽  
Reny Rosalina
Keyword(s):  
Body Weight ◽  
Optimal Dose ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Antioxidant Compounds ◽  
Optimal Dosage ◽  
Skin Injury ◽  
Treatment Groups

Introduction: In humans, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) can damage some tissue when the immune systems was decrease. Natural product from the plant often used to improve immune response against microorganism including virus. This study aimed to determine the potential antioxidant of lemongrass extract (C . nardus) with various dosage that can provide immunomodulatory effects and find an optimal dosage to be used.  Methods: The method used observasional analytic, using animal model of 30 male mice strain BALB/C, weight 25-30 gram, divided into 5 groups; the positive control group was given 0.05 mL of  0.05% CMC within 14 days, negative control group was given IMBOOST® tablet 200 mg/kg body weight (bw) within 14 days, treatment groups  were given C. nardus extract with various doses 50 mg/kg bw, 150 mg/kg bw, and 300 mg/kg bw.  In day 21 all group were injected with 0,2 ml of  pathogen bacterial (S. aureus). Blood samples were taken three times: 7th day, 14th day, and 21th day. Results: The results showed that lemongrass extract (C. nardus) was able to influence the leukocyte and lymphocytes count with significant different (p<0.05). The optimal dose is 150 mg/kg body weight. Conclusion: The antioxidant compounds that contain in the C. nardus extract have an ability to increasing the immune system in the dose 150 mg/kg bw , but in the dose 300 mg/kg bw became toxic that can make a skin injury or death in animal test.

Effects of Curcuma Zedoaria (Shoti) on Growth Performance and Hemato-Biochemical Parameters in Broiler

2020 ◽  
Vol 7 (2) ◽  
pp. 293-301
Author(s):  
TM Rahsin Kabir ◽  
Nahid Nawrin Sultana ◽  
Tangila Ferdausi ◽  
Muhammad Rakibul Hasan ◽  
Nayamul Bashar ◽  
...  
Keyword(s):  
Body Weight ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Broiler Chicks ◽  
Collect Blood Sample ◽  
Growth Performances

The experiment was conducted on “Cobb-500” broiler chicks to study the effects of Curcumazedoaria (shoti) on growth performances and haemato-biochemical parameters. A total of twentybroilers chicks (16 days old) were randomly divided into five equal groups (n=4). Three groups ofbroilers fed shoti powder at the rate of 10%; 20%; 30% (w/w) with other ingredients for 22 days.Broilers chicks fed on maize-soybean were considered as positive control while non-protein controlwas regarded as negative control. The results showed that body weight of broilers fed 10% and 20%shoti meal grew very close to those of control (maize-soybean) group. After having completed thefeeding trial, the birds were sacrificed to collect blood sample for and biochemical analysis .Weightsof different organs and length of intestine were also measured. Results showed that decreaseddressed weight, weight of skin and liver were found in 30% shoti treated group compared to proteincontrol group. Intestinal weight was highest in 30% shoti treated group but length was similar to theprotein control group. Total erythrocytes count, hemoglobin content and PCV value were founddecreased in shoti treated group compared to protein control group but ESR value was highest in10% shoti treated group. Total cholesterol was found to be decreased in the birds fed on shoticompared to protein control and significantly higher than non-protein control group. Triglyceride valuedecreased in 10% shoti treated group compared to protein and non-protein control group. IncreasedHDL value was found in 10% shoti treated group compared to control groups and 20 % and 30 %shoti treated groups. Result of this experiment showed that body weight of birds significantly (p<0.05)increased in all shoti treated groups. Further investigations are necessary by using differentpercentages of shoti and other shoti type feed to determine the effects of this feed supplement onanimal’s physiology. Res. Agric., Livest. Fish.7(2): 293-301,  August 2020

scholarly journals Evaluation of Carrot Pomace (Daucus carota L.) as Hypocholesterolemic and Hypolipidemic Agent on Albino Rats

2013 ◽  
Vol 5 (1) ◽  
pp. 7-14 ◽  
Author(s):  
Abd El-Moneim M.R. AFIFY ◽  
Ramy R.M ROMEILAH ◽  
Mahmoud M.H. OSFOR ◽  
Amir S.M. ELBAHNASAWY
Keyword(s):  
Body Weight ◽  
Body Weight Gain ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Control Group ◽  
Albino Rats ◽  
Obese People ◽  
Daucus Carota L ◽  
Hypercholesterolemic Diet

The current study examined the attenuating influence of dietary carrot pomace powder (CaPP) on hypercholesterolemia and various oxidative stress-associated with biochemical parameters in hypercholesterolemic rats. Thirty two male albino rats weighing 110±10 g were divided into four groups, the first group received the basal diet only and served as (negative control), the second group received the hypercholesterolemic diet and served as positive control, the other groups received hypercholesterolemic diet supplemented with 10%, 20% CaPP for six weeks. The obtained results revealed that groups supplemented with 10% and 20% CaPP significantly decrease total lipid, total cholesterol, triglycerides, low density lipoprotein cholesterol, liver enzymes: alanine aminotransferase, aspartate aminotransferase compared to positive and negative groups. Organs weight, body weight gain significantly decreased compared with positive control. Moreover dietary carrot pomace powder can used to reduce the body weight and reducing hypercholesterolemic complications. In addition, dietary carrot pomace powder serves to improve the blood picture and to reduce the blood glucose level in hypercholesterolemic rats and could use in obese people for body loss. Data of kidney function (Urea) record an increase in CaPP 20% level (26.9±2.96) but this increase was non significant with the negative control group (26.6±3.1).

Hepatoprotective Role of AV/LCP/18 In CCl4 Induced FLKS Syndrome in Commercial Broilerst

2016 ◽  
Vol 12 (2) ◽  
Author(s):  
P. M. Sonkusale ◽  
Pratik N. Inglepatil ◽  
Siddharth V. Jamadade
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Protein Profile ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Broiler Chicks ◽  
Mean Values ◽  
Group B

A total of 75 day old broiler chicks were divided into three equal groups. Group A served as negative control. Group B (positive control) and C (treatment group) were intoxicated with oral administration of CCl4 @ 1 ml/kg body weight after every 3rd day during 15-28 days of age. Group C was given AV/LCP/18 @ 0.10 ml / chick / day in drinking water during 29th-42nd day of age. At the end of experiment, significantly (P is less than 0.01) higher mean body weight and lower FCR was observed in treatment group C as compared to positive control group B. Similarly, significantly (P is less than 0.05) decreased levels of SGOT, SGPT, cholesterol, triglycerides, creatinine and higher mean values of serum protein profile along with mild degenerative changes in liver and kidney of broilers in group C were observed as compared to group B indicating therapeutic efficacy of AV/LCP/18 in ameliorating the toxic effects or FLKS induced by CCl4 in commercial broilers

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