scholarly journals Draft Genome Sequence of the Novel Enterobacter cloacae Strain amazonensis, a Highly Heavy Metal-Resistant Bacterium from a Contaminated Stream in Amazonas, Brazil

2018 ◽  
Vol 6 (22) ◽  
Author(s):  
Maria Clara Tavares Astolfi ◽  
Elen Bethleen de Souza Carvalho ◽  
Adriane Menezes de Barros ◽  
Marcelo Valente Pinto ◽  
Luna Barrôco de Lacerda ◽  
...  

ABSTRACT Here, we report the draft genome of the Enterobacter cloacae strain amazonensis, a bacterium highly resistant to mercury that was isolated from a metal- and sewage-contaminated stream in Amazonas, Brazil. The exploration of the 5.0-Mb genome revealed 104 genes encoding resistance to toxic compounds and heavy metals, highlighting the potential biotechnological applications of this strain.

2021 ◽  
Vol 10 (16) ◽  
Author(s):  
Thaiane Defalco ◽  
Ana L. S. Vasconcelos ◽  
Armando C. F. Dias ◽  
Leticia Barrientos ◽  
Ângelo F. Bernardino ◽  
...  

Bacillus safensis 3A was isolated from a contaminated estuarine sediment sample with mine tailing from the Samarco dam disaster, which occurred in 2015 in Minas Gerais State, Brazil. We report here a draft genome sequence (3.6 Mb) of this bacterial strain. B. safensis exhibited strong resistance to heavy metals.


2017 ◽  
Vol 5 (33) ◽  
Author(s):  
Lex E. X. Leong ◽  
David Shaw ◽  
Lito Papanicolas ◽  
Diana Lagana ◽  
Ivan Bastian ◽  
...  

ABSTRACT Enterobacter cloacae is a common member of the gut microbiota in healthy individuals. However, it is also an opportunistic pathogen, capable of causing bacteremia. We report the draft genomes of two Enterobacter cloacae subspecies cloacae strains isolated from hematology patients with bacteremia. Both isolates carry genes encoding extended-spectrum β-lactamases.


2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Gisele Nunes Rodrigues ◽  
Asunción Lago-Lestón ◽  
Rodrigo Costa ◽  
Tina Keller-Costa

ABSTRACT We report here the genome sequence of Labrenzia sp. EL143, an alphaproteobacterium isolated from the gorgonian coral Eunicella labiata that possesses various genes involved in halogen and aromatic compound degradation, as well as polyketide synthesis. The strain also maintains multiple genes that confer resistance to toxic compounds such as heavy metals and antibiotics.


2019 ◽  
Vol 8 (49) ◽  
Author(s):  
Luis Tataje-Lavanda ◽  
Phillip Ormeño-Vásquez ◽  
Rosa Altamirano-Díaz ◽  
Lucy Espinoza-Salazar ◽  
Mirko Zimic ◽  
...  

Here, we report a draft genome sequence of Aeromonas veronii strain CTe-01 (4.5 Mb), a hemolytic, heavy metal-resistant bacterium isolated from a wastewater treatment plant located at Cachiche, Ica, Peru. These characteristics could be used for bioremediation of contaminated environments.


2014 ◽  
Vol 58 (9) ◽  
pp. 5589-5593 ◽  
Author(s):  
Anna L. Sartor ◽  
Muhammad W. Raza ◽  
Shahid A. Abbasi ◽  
Kathryn M. Day ◽  
John D. Perry ◽  
...  

ABSTRACTThe molecular epidemiology of 66 NDM-producing isolates from 2 Pakistani hospitals was investigated, with their genetic relatedness determined using repetitive sequence-based PCR (Rep-PCR). PCR-based replicon typing and screening for antibiotic resistance genes encoding carbapenemases, other β-lactamases, and 16S methylases were also performed. Rep-PCR suggested a clonal spread ofEnterobacter cloacaeandEscherichia coli. A number of plasmid replicon types were identified, with the incompatibility A/C group (IncA/C) being the most common (78%). 16S methylase-encoding genes were coharbored in 81% of NDM-producingEnterobacteriaceae.


2018 ◽  
Vol 7 (1) ◽  
pp. 28-40
Author(s):  
Bikram Gautam ◽  
Rameshwar Adhikari

Wastewater treatment plant is a potential reservoir contributing to the evolution and spread of heavy metal and antibiotic resistant bacteria. The pollutants such as biocides, antibiotics, heavy metals are to be feared for as they have been known to evoke resistance in microorganisms in such polluted environment. The aim of this study was to the isolate bacteria from the treated wastewater and assess the resistance pattern of the isolates against antibiotics and heavy metals. Grab sampling was performed from April to June 2017, from the treated effluent from the secondary treatment plant. To assess the resistance pattern for antibiotic(s) and heavy metal(s), antibiotic susceptibility test and minimum inhibitory concentration by cup well method were performed respectively. Staphylococcus aureus, Enterococcus faecalis, Citrobacter freundii, Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, P. vulgaris, Salmonella Typhi, Pseudomonas aeruginosa were isolated. Multi drug and heavy metal resistant isolates were screened. Fisher’s exact test revealed that there is a significant association (p< 0.001) between antibiotic resistance pattern and resistance patterns at dilution of 2500 g/L (25%). Cramer’s V test revealed that the effect size of antibiotic resistance pattern and heavy metal resistance pattern at dilution 2500 g/L is medium. P. aeruginosa was able to resist the metal concentration up to 10000 g/L (100%) dilution of Fe++. Heavy metal resistant bacteria can be safely used to lower chemical concentration in the environment once their harmful genes are edited, knocked etc. so that risks of evoking antibiotic resistance could be minimized. 


Author(s):  
Bronius Jaskelevičius ◽  
Vaida Lynikienė

As a result of global and intense production the waste disposal problems become more and more urgent. Waste processing, utilization and recycling is to a certain extent limited by many economic, organisational and technological factors, and this inevitably encourages waste disposal in landfills. Physical, chemical and biological interactions in landfill cell result in formation of landfill gas and harmful leachate. Because of lack of control, together with usual communal waste, industrial waste was also dumped to landfills, therefore gas and leachate produced include large amounts of toxic compounds. Once hazardous waste materials occured in landfills, later they vastly expanded the whole spectrum of toxic materials and compounds. In the landfill environment chemical properties of surface and ground water and concentration of separate components are governed by seepage of leachate and industrial solutants into soil and ground layers and their transport by subsurface waters. Influence on the environment exerted by heavy metals contained in the leachate of Lapes Landfill is discussed in this paper. Properties of industrial waste material influenced order of the main pollutants: the most important elements in this case are Cu, Ni, Zn, Pb, Mn, Cr and other ions, the sulphides of these metals and other toxic compounds. The First Landfill field is more polluted with heavy metal polutants than the Third field. In all the samples iron concentration is the greatest exceeding even 200 times the admissible value allowed (Norm HN 24:2003). Sources (springs) S11 and S17 are least contaminated with heavy metals. The greatest groundwater pollution was found in monitoring bore G13s. The leachate processed in purification devices is released to the Third stream. Heavy metal concentrations in waters of this stream are low and they further decrease downstream because the pollutants are diluted. Santrauka Masiškai gaminant produkciją, ją vartojant, vis opesnė tampa atliekų problema. Atliekų perdirbimą, utilizavimą iš dalies ribojantys ekonominiai, organizaciniai bei technologiniai veiksniai neišvengiamai skatina atliekas šalinti į sąvartynus. Dėl sąvartyno tūryje vykstančių fizinių, cheminių bei biologinių reakcijų susidaro sąvartyno dujos ir kenksmingas filtratas. Kadangi dėl nepakankamos kontrolės į sąvartynus kartu su buitinėmis, komunalinėmis atliekomis buvo šalinamos pramonės atliekos, susidarančiose dujose bei filtrate yra daug toksiškų junginių. Šią toksinių medžiagų bei junginių įvairovę dar labiau papildo į sąvartynus patenkančios pavojingos atliekos. Gruntinio ir paviršinio upelių vandens cheminę sudėtį ir kai kurių komponentų koncentraciją sąvartyno aplinkoje lemia filtrato ir pramoninių tirpalų įsisunkimo į gruntą mastas bei požeminių tėkmių pernašos. Straipsnyje nagrinėjama Lapių sąvartyno filtrate aptiktų sunkiųjų metalų įtaka aplinkai. Pramonės atliekos lėmė, kad filtrato pagrindiniai teršiantieji elementai yra Cu, Ni, Zn, Pb, Mn, Cr ir kt. jonai, šių metalų sulfidai ir kiti toksiniai junginiai. Pirmasis kaupimosi laukas yra labiau užterštas sunkiaisiais metalais nei trečiasis laukas. Visuose mėginiuose didžiausia yra geležies koncentracija. Ji net iki 200 kartų viršija HN 24:2003 leidžiamąją normą. Mažiausiai sunkiaisiais metalais užteršti šaltiniai (S11 ir S17 postai). Požeminis vanduo labiausiai užterštas G13s gręžinyje. Iš valymo įrenginių išvalytas filtratas yra išleidžiamas į upelį. Šio upelio vandenyje rastų sunkiųjų metalų koncentracijos yra nedidelės, o upeliui tekant tolyn teršalai atskiedžiami, ir metalų koncentracijos mažėja. Резюме Массовое производство продукции, пользование ею все более обостряют проблему отходов. Процессы переработки и утилизации отходов, в определенной степени ограничивающие экономические, организационные и технологические факторы, неизбежно способствуют интенсификации удаления отходов на свалки. На свалках отходов в результате протекающих физических, химических и биологических реакций образуются газы и токсичный фильтрат. Поскольку из-за недостаточного контроля на свалку вместе с бытовыми, коммунальными отходами удалялись и промышленные отходы, в составе образующихся газов и фильтрата имеется много токсичных соединений. Опасные отходы еще более увеличили спектр токсичных материалов и соединений в газах и фильтрате свалки. Химический состав и концентрация отдельных компонентов грунтовых и поверхностных вод в районе свалки определяют проникновение фильтрата и промышленных растворов в грунт и их перенос подземными течениями. В статье изучается влияние тяжелых металлов из фильтрата свалки в Лапес на состояние вод в районе свалки. Промышленные отходы способствовали появлению в фильтрате ионов основных загрязняющих элементов Cu, Ni, Zn, Pb, Mn, Cr, сульфидов этих металлов и других токсичных соединений. Первое поле накопления отходов в большей степени загрязнено тяжелыми металлами, чем третье поле. Во всех опытных образцах воды отмечена самая большая концентрация ионов железа, почти в 200 раз превышающая допустимую норму HN 24:2003. Наименьшее загрязнение тяжелыми металлами отмечено в подземной воде источников (посты S11 и S17). Грунтовые воды больше всего загрязнены в скважине G13s. Поверхностные воды ручьев, в которые проникает фильтрат, а также сливается очищенный фильтрат, содержат небольшие концентрации тяжелых металлов, которые разбавляются течением и уменьшаются.


2018 ◽  
Vol 7 (16) ◽  
Author(s):  
Christopher R. Dumigan ◽  
Gregory E. Perry ◽  
K. Peter Pauls ◽  
Manish N. Raizada

Presented here is the draft genome sequence of Enterobacter cloacae 3D9. This candidate seed endophyte was isolated from Zea nicaraguensis.


2020 ◽  
Vol 9 (37) ◽  
Author(s):  
Israel García-Cano ◽  
Walaa E. Hussein ◽  
Diana Rocha-Mendoza ◽  
Ahmed E. Yousef ◽  
Rafael Jiménez-Flores

ABSTRACT The novel strain Lactobacillus rhamnosus OSU-PECh-69 was isolated from provolone cheese. It produces antimicrobial agents having a molecular mass of 5 to 10 kDa that are active against Gram-positive and Gram-negative bacteria. The strain has a genome sequence of 3,057,669 bp, a GC content of 46.6%, and up to two gene clusters encoding bacteriocins.


2019 ◽  
Vol 63 (6) ◽  
Author(s):  
Michael S. M. Brouwer ◽  
Kamaleddin H. M. E. Tehrani ◽  
Michel Rapallini ◽  
Yvon Geurts ◽  
Arie Kant ◽  
...  

ABSTRACT Food for human consumption is screened widely for the presence of antibiotic-resistant bacteria to assess the potential for transfer of resistant bacteria to the general population. Here, we describe an Enterobacter cloacae complex isolated from imported seafood that encodes two carbapenemases on two distinct plasmids. Both enzymes belong to Ambler class A β-lactamases, the previously described IMI-2 and a novel family designated FLC-1. The hydrolytic activity of the novel enzyme against aminopenicillins, cephalosporins, and carbapenems was determined.


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