scholarly journals Contribution of Polycomb Homologues Bmi-1 and Mel-18 to Medulloblastoma Pathogenesis

2007 ◽  
Vol 27 (13) ◽  
pp. 4968-4979 ◽  
Author(s):  
Dmitri Wiederschain ◽  
Lin Chen ◽  
Brett Johnson ◽  
Kimberly Bettano ◽  
Dowdy Jackson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Growth ◽  
Cancer Cell ◽  
Protein Complexes ◽  
Clonogenic Survival ◽  
Tumor Formation ◽  
Polycomb Group ◽  
Cancer Cell Growth ◽  
Inhibition Of Proliferation ◽  
Polycomb Proteins

ABSTRACT Bmi-1 and Mel-18 are structural homologues that belong to the Polycomb group of transcriptional regulators and are believed to stably maintain repression of gene expression by altering the state of chromatin at specific promoters. While a number of clinical and experimental observations have implicated Bmi-1 in human tumorigenesis, the role of Mel-18 in cancer cell growth has not been investigated. We report here that short hairpin RNA-mediated knockdown of either Bmi-1 or Mel-18 in human medulloblastoma DAOY cells results in the inhibition of proliferation, loss of clonogenic survival, anchorage-independent growth, and suppression of tumor formation in nude mice. Furthermore, overexpression of both Bmi-1 and Mel-18 significantly increases the clonogenic survival of Rat1 fibroblasts. In contrast, stable downregulation of Bmi-1 or Mel-18 alone does not affect the growth of normal human WI38 fibroblasts. Proteomics-based characterization of Bmi-1 and Mel-18 protein complexes isolated from cancer cells revealed substantial similarities in their respective compositions. Finally, gene expression analysis identified a number of cancer-relevant pathways that may be controlled by Bmi-1 and Mel-18 and also showed that these Polycomb proteins regulate a set of common gene targets. Taken together, these results suggest that Bmi-1 and Mel-18 may have overlapping functions in cancer cell growth.

scholarly journals HOXB4 inhibits the proliferation and tumorigenesis of cervical cancer cells by downregulating the activity of Wnt/β-catenin signaling pathway

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Dan Lei ◽  
Wen-Ting Yang ◽  
Peng-Sheng Zheng
Keyword(s):  
Cervical Cancer ◽  
Cell Growth ◽  
Signaling Pathway ◽  
Cancer Cell ◽  
Tumor Formation ◽  
Cervical Cancer Cell ◽  
Cancer Cell Growth ◽  
Bioinformatics Analyses

AbstractHomeobox B4 (HOXB4), which belongs to the homeobox (HOX) family, possesses transcription factor activity and has a crucial role in stem cell self-renewal and tumorigenesis. However, its biological function and exact mechanism in cervical cancer remain unknown. Here, we found that HOXB4 was markedly downregulated in cervical cancer. We demonstrated that HOXB4 obviously suppressed cervical cancer cell proliferation and tumorigenic potential in nude mice. Additionally, HOXB4-induced cell cycle arrest at the transition from the G0/G1 phase to the S phase. Conversely, loss of HOXB4 promoted cervical cancer cell growth both in vitro and in vivo. Bioinformatics analyses and mechanistic studies revealed that HOXB4 inhibited the activity of the Wnt/β-catenin signaling pathway by direct transcriptional repression of β-catenin. Furthermore, β-catenin re-expression rescued HOXB4-induced cervical cancer cell defects. Taken together, these findings suggested that HOXB4 directly transcriptional repressed β-catenin and subsequently inactivated the Wnt/β-catenin signaling pathway, leading to significant inhibition of cervical cancer cell growth and tumor formation.

scholarly journals A Coactivator Role of CARM1 in the Dysregulation of β-Catenin Activity in Colorectal Cancer Cell Growth and Gene Expression

2011 ◽  
Vol 9 (5) ◽  
pp. 660-670 ◽  
Author(s):  
Chen-Yin Ou ◽  
Melissa J. LaBonte ◽  
Philipp C. Manegold ◽  
Alex Yick-Lun So ◽  
Irina Ianculescu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Cell Growth ◽  
Cancer Cell ◽  
Colorectal Cancer Cell ◽  
Cancer Cell Growth

scholarly journals Anticancer Effects of Lingonberry and Bilberry on Digestive Tract Cancers

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 850
Author(s):  
Tuulia Onali ◽  
Anne Kivimäki ◽  
Matti Mauramo ◽  
Tuula Salo ◽  
Riitta Korpela
Keyword(s):  
Colorectal Cancer ◽  
Cell Growth ◽  
Digestive Tract ◽  
Cancer Cell ◽  
Tumor Formation ◽  
Cancer Cell Growth ◽  
Antitumor Effects ◽  
Anticancer Effects ◽  
Wild Berries

Wild berries are part of traditional Nordic diets and are a rich source of phytochemicals, such as polyphenols. Various berry treatments have shown to interfere with cancer progression in vitro and in vivo. Here, we systematically reviewed the anticancer effects of two Nordic wild berries of the Vaccinium genus, lingonberry (Vaccinium vitis-idaea) and bilberry (Vaccinium myrtillus), on digestive tract cancers. The review was conducted according to the PRISMA 2020 guidelines. Searches included four databases: PubMed, Scopus, Web of Science, and CAB abstracts. Publications not written in English, case-reports, reviews, and conference abstracts were excluded. Moreover, studies with only indirect markers of cancer risk or studies with single compounds not derived from lingonberry or bilberry were not included. Meta-analysis was not performed. The majority (21/26) of studies investigated bilberry and colorectal cancer. Experimental studies on colorectal cancer indicated that bilberry inhibited intestinal tumor formation and cancer cell growth. One uncontrolled pilot human study supported the inhibitory potential of bilberry on colorectal cancer cell proliferation. Data from all 10 lingonberry studies suggests potent inhibition of cancer cell growth and tumor formation. In conclusion, in vitro and animal models support the antiproliferative and antitumor effects of various bilberry and lingonberry preparations on digestive tract cancers.

scholarly journals Spatial control of oxygen delivery to three‐dimensional cultures alters cancer cell growth and gene expression

2019 ◽  
Vol 234 (11) ◽  
pp. 20608-20622 ◽  
Author(s):  
William J. Wulftange ◽  
Michelle A. Rose ◽  
Marcial Garmendia‐Cedillos ◽  
Davi da Silva ◽  
Joanna E. Poprawski ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Growth ◽  
Cancer Cell ◽  
Oxygen Delivery ◽  
Three Dimensional ◽  
Cancer Cell Growth ◽  
Spatial Control

scholarly journals Circadian clock gene expression regulates cancer cell growth through glutaminase

2014 ◽  
Vol 46 (5) ◽  
pp. 409-414 ◽  
Author(s):  
A. Huang ◽  
B. Bao ◽  
H. R. Gaskins ◽  
H. Liu ◽  
X. Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Growth ◽  
Circadian Clock ◽  
Cancer Cell ◽  
Clock Gene ◽  
Cancer Cell Growth ◽  
Circadian Clock Gene ◽  
Clock Gene Expression

scholarly journals Simvastatin inhibits cancer cell growth by inducing apoptosis correlated to activation of Bax and down-regulation of BCL-2 gene expression

2011 ◽  
Vol 40 (4) ◽  
pp. 935-941 ◽  
Author(s):  
CARMINE SPAMPANATO ◽  
SALVATORE DE MARIA ◽  
MADDALENA SARNATARO ◽  
ELISABETTA GIORDANO ◽  
MARIO ZANFARDINO ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Growth ◽  
Cancer Cell ◽  
Cancer Cell Growth ◽  
Down Regulation

scholarly journals The Potential of 9,10-Anthraquinone in Inhibiting Human Cancer Cells Growth

2021 ◽  
Vol 15 (1) ◽  
pp. 19
Author(s):  
Irmanida Batubara ◽  
Arif Rakhman Hakim ◽  
Silmi Mariya ◽  
Suminar Setiati Achmadi ◽  
Valentina Sokoastri Valentina Sokoastri ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Growth ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Hela Cells ◽  
Human Cancer ◽  
Cancer Cell Growth ◽  
Human Cancer Cells ◽  
Hct 116 ◽  
Mcf 7

Background: 9,10-Anthraquinone (9,10-AQ) is a contaminant on some agricultural products and considered as carcinogenic based on EU Regulation No. 1146/2014. Except for little evidence on experimental rats, there is no strong proof regarding the carcinogenicity in humans. Therefore, it is essential to find a safe dose of this compound since the difference in 9,10-AQ levels will affect cancer cell growth. This research aims to find the 9,10-AQ concentration that does not proliferate the human cancer cells under in vitro study.Methods: In determining the 9,10-AQ concentration that does not proliferate the cancer cells growth, 0.01 to 500 mg/L 9,10-AQ was directly tested on four human cancer cells (colorectal carcinoma HCT 116, colon adenocarcinoma WiDr, breast cancer MCF-7, and cervical cancer HeLa), and the viability of the cells was counted via (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay. In the gene expression level, the effects on a selected cancer cell line were determined by qRT-PCR against BAX, BCL-2, PCNA, and P53.Results: The result indicates that 9,10-AQ up to 500 mg/L concentration does not proliferate the cell’s growth but instead inhibits those four cancer cells’ growths. The concentration of 9,10-AQ that inhibits 50% the cancer cells growth (IC50) value was 321.8 mg/L (1.55 mM) against HCT 116 and above 500 mg/L (above 2.40 mM) against WiDr, MCF-7, and HeLa. The 9,10-AQ at 500 mg/L (or 2.40 mM) increases BAX expression and acts as an apoptotic agent on HeLa cells.Conclusions: The investigation has shown that 9,10-AQ up to 500 mg/L concentration does not proliferate the cancer cell growth; instead, it inhibits the HCT 116 and HeLa cells growth. We have preliminary evidence regarding the apoptotic mechanism of 9,10-AQ by increasing BAX gene expression on HeLa cells.

The polycomb group protein EZH2 inhibits lung cancer cell growth by repressing the transcription factor Nrf2

FEBS Letters ◽  
2014 ◽  
Vol 588 (17) ◽  
pp. 3000-3007 ◽  
Author(s):  
Ziming Li ◽  
Ling Xu ◽  
Naiwang Tang ◽  
Yunhua Xu ◽  
Xiangyun Ye ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Transcription Factor ◽  
Cell Growth ◽  
Cancer Cell ◽  
Polycomb Group ◽  
Polycomb Group Protein ◽  
Lung Cancer Cell ◽  
Cancer Cell Growth ◽  
Group Protein
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