scholarly journals Analysis of Spt7 Function in the Saccharomyces cerevisiae SAGA Coactivator Complex

2002 ◽  
Vol 22 (15) ◽  
pp. 5367-5379 ◽  
Author(s):  
Pei-Yun Jenny Wu ◽  
Fred Winston
Keyword(s):  
Saccharomyces Cerevisiae ◽  
The Other ◽  
Deletion Mutants ◽  
Saga Complex ◽  
Number Of Genes ◽  
Processed Form ◽  
Altered Form ◽  
And Function ◽  
Coactivator Complex

ABSTRACT The Saccharomyces cerevisiae SAGA complex is required for the normal transcription of a large number of genes. Complex integrity depends on three core subunits, Spt7, Spt20, and Ada1. We have investigated the role of Spt7 in the assembly and function of SAGA. Our results show that Spt7 is important in controlling the levels of the other core subunits and therefore of SAGA. In addition, partial SAGA complexes containing Spt7 can be assembled in the absence of both Spt20 and Ada1. Through biochemical and genetic analyses of a series of spt7 deletion mutants, we have identified a region of Spt7 required for interaction with the SAGA component Spt8. An adjacent Spt7 domain was found to be required for a processed form of Spt7 that is present in a previously identified altered form of SAGA called SLIK, SAGAalt, or SALSA. Analysis of an spt7 mutant with greatly reduced levels of SLIK/SAGAalt/SALSA suggests a subtle role for this complex in transcription that may be redundant with a subset of SAGA functions.

scholarly journals Knockout of Arabidopsis thaliana VEP1, Encoding a PRISE (Progesterone 5β-Reductase/Iridoid Synthase-Like Enzyme), Leads to Metabolic Changes in Response to Exogenous Methyl Vinyl Ketone (MVK)

Metabolites ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 11
Author(s):  
Jan Klein ◽  
Mona Ernst ◽  
Alexander Christmann ◽  
Marina Tropper ◽  
Tim Leykauf ◽  
...  
Keyword(s):  
Metabolic Networks ◽  
Vinyl Ketone ◽  
Methyl Vinyl Ketone ◽  
General Role ◽  
Methyl Vinyl ◽  
Knockout Mutants ◽  
Number Of Genes ◽  
Vein Patterning ◽  
And Function

Small or specialized natural products (SNAPs) produced by plants vary greatly in structure and function, leading to selective advantages during evolution. With a limited number of genes available, a high promiscuity of the enzymes involved allows the generation of a broad range of SNAPs in complex metabolic networks. Comparative metabolic studies may help to understand why—or why not—certain SNAPs are produced in plants. Here, we used the wound-induced, vein patterning regulating VEP1 (AtStR1, At4g24220) and its paralogue gene on locus At5g58750 (AtStR2) from Arabidopsis to study this issue. The enzymes encoded by VEP1-like genes were clustered under the term PRISEs (progesterone 5β-reductase/iridoid synthase-like enzymes) as it was previously demonstrated that they are involved in cardenolide and/or iridoid biosynthesis in other plants. In order to further understand the general role of PRISEs and to detect additional more “accidental” roles we herein characterized A. thaliana steroid reductase 1 (AtStR1) and compared it to A. thaliana steroid reductase 2 (AtStR2). We used A. thaliana Col-0 wildtype plants as well as VEP1 knockout mutants and VEP1 knockout mutants overexpressing either AtStR1 or AtStR2 to investigate the effects on vein patterning and on the stress response after treatment with methyl vinyl ketone (MVK). Our results added evidence to the assumption that AtStR1 and AtStR2, as well as PRISEs in general, play specific roles in stress and defense situations and may be responsible for sudden metabolic shifts.

Indigenous approaches to the past: ‘Creative histories’ at the Hyde Park Barracks, Sydney

2020 ◽  
Vol 9 (1) ◽  
pp. 83-102 ◽  
Author(s):  
Kiera Lindsey
Keyword(s):  
Visual Art ◽  
The Other ◽  
Cultural Relations ◽  
Form And Function ◽  
The Past ◽  
Cultural Responses ◽  
And Function ◽  
The Way

This article discusses a recent art project created by the Wiradjuri and Kamilaroi artist Jonathon Jones, which was commissioned to commemorate the opening of the revitalized Hyde Park Barracks in Sydney in early 2020. Jones’ work involves a dramatic installation of red and white crushed stones laid throughout the grounds of the barracks, merging the image of the emu footprint with that of the English broad convict arrow to ‘consider Australia’s layered history and contemporary cultural relations’. This work was accompanied by a ‘specially-curated programme’ of performances, workshops, storytelling and Artist Talks. Together, these elements were designed to unpack how certain ‘stories determine the ways we came together as a nation’. As one of the speakers of the Artist Talk’s programme, I had a unique opportunity to experiment with what colleagues and I have been calling ‘Creative histories’ in reference to the way some artists and historians are choosing to communicate their research about the past in ways that experiment with form and function and push disciplinary or generic boundaries. This article reflects upon how these two distinct creative history projects – one visual art, the other performative – renegotiate the complex and contested pasts of the Hyde Park Barracks. I suggest that both examples speak to the role of memory and creativity in shaping cultural responses to Australia’s colonial past, while Jones' programme illustrates how Indigenous artists and academics are making a profound intervention into contemporary understandings of how history is ‘done’ in Australia.

scholarly journals C-terminus of the P4-ATPase ATP8A2 functions in protein folding and regulation of phospholipid flippase activity

2017 ◽  
Vol 28 (3) ◽  
pp. 452-462 ◽  
Author(s):  
Madhavan Chalat ◽  
Kody Moleschi ◽  
Robert S. Molday
Keyword(s):  
Endoplasmic Reticulum ◽  
Atpase Activity ◽  
Deletion Mutants ◽  
Serine Residue ◽  
Cellular Processes ◽  
Phospholipid Asymmetry ◽  
C Terminus ◽  
And Function ◽  
Phospholipid Flippase

ATP8A2 is a P4-ATPase that flips phosphatidylserine and phosphatidylethanolamine across cell membranes. This generates membrane phospholipid asymmetry, a property important in many cellular processes, including vesicle trafficking. ATP8A2 deficiency causes severe neurodegenerative diseases. We investigated the role of the C-terminus of ATP8A2 in its expression, subcellular localization, interaction with its subunit CDC50A, and function as a phosphatidylserine flippase. C-terminal deletion mutants exhibited a reduced tendency to solubilize in mild detergent and exit the endoplasmic reticulum. The solubilized protein, however, assembled with CDC50A and displayed phosphatidylserine flippase activity. Deletion of the C-terminal 33 residues resulted in reduced phosphatidylserine-dependent ATPase activity, phosphatidylserine flippase activity, and neurite extension in PC12 cells. These reduced activities were reversed with 60- and 80-residue C-terminal deletions. Unlike the yeast P4-ATPase Drs2, ATP8A2 is not regulated by phosphoinositides but undergoes phosphorylation on the serine residue within a CaMKII target motif. We propose a model in which the C-terminus of ATP8A2 consists of an autoinhibitor domain upstream of the C-terminal 33 residues and an anti-autoinhibitor domain at the extreme C-terminus. The latter blocks the inhibitory activity of the autoinhibitor domain. We conclude that the C-terminus plays an important role in the efficient folding and regulation of ATP8A2.

scholarly journals The Polyphony of the Silent: The Specifics of Dostoevsky’s Philosophical Discourse

2021 ◽  
Vol 26 (3) ◽  
pp. 375-391
Author(s):  
Alexandr B. Krinitsyn
Keyword(s):  
Psychological Aspects ◽  
The Other ◽  
Philosophical Discourse ◽  
And Function

The article describes the phenomenon of silence in Dostoevskys later prose. Philosophical, plot-forming and psychological aspects of silence are looked at. A confessional type of monologue is defined as a discursive genre, with specific roles of the speaker and his silent listeners, whose predominance in Dostoevskys prose lets the author of the article adjust the notion of polyphony and dialogue within the context of Dostoevskys poetics. The conventionality of the other in the confessional type of monologue is proved by the absence of the completing functions in listeners. In most cases Dostoevskys later works retain some rudimental forms of frantic dialogue while the role of specific monologue discourse, caused and conditioned by silence, is increasing. By semantics and function types of silence in Dostoevsky fall into three groups: 1) the underground silence as expression of human disunity in the epoch of universal solitude; 2) role silence of listeners in the discursive genre of confessional monologue; 3) sacred silence of Christ indicating transition to a new higher level of communication

scholarly journals Bacterial Diversity and Community in Regional Water Microbiota between Different Towns in World’s Longevity Township Jiaoling, China

Diversity ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 361
Author(s):  
Lei Wu ◽  
Xinqiang Xie ◽  
Jumei Zhang ◽  
Yu Ding ◽  
Qingping Wu
Keyword(s):  
16S Rrna ◽  
High Throughput Sequencing ◽  
The Other ◽  
Rrna Gene ◽  
Metabolic Characteristics ◽  
Kegg Pathways ◽  
Healthy Longevity ◽  
And Function ◽  
Regional Water

Healthy longevity is associated with many factors, however, the potential correlation between longevity and microbiota remains elusive. To address this, we explored environmental microbiota from one of the world’s longevity townships in China. We used 16S rRNA gene high-throughput sequencing to analyze the composition and function of water microbiota. The composition and diversity of water microbiota significantly differed between the towns. Lactobacillus, Streptococcus, Bacteroides, Faecalibacterium, and Stenotrophomonas were only dominant in Xinpu, a town with an exceptionally high centenarian population. Several biomarkers were identified, including Flavobacterium, Acinetobacter, Paracoccus, Lactobacillales, Psychrobacter, Bacteroides, Ruminococcaceae, and Faecalibacterium, and these shown to be responsible for the significant differences between towns. The main species contributing to the differences between towns were Cyanobacteria, Cupriavidus and Ralstonia. Based on KEGG pathways showed that the predicted metabolic characteristics of the water microbiota in Xinpu towns were significantly different to those of the other towns. The results revealed significant differences in the composition and diversity of water microbiota in the longevity township. These findings provide a foundation for further research on the role of water microbiota in healthy longevity.

scholarly journals Is English a Killer Language or an International Auxiliary? Its Use and Function in a Globalised World

2012 ◽  
Vol 1 ◽  
pp. 9 ◽  
Author(s):  
Nick Ceramella
Keyword(s):  
British Empire ◽  
English Language ◽  
Mother Tongue ◽  
The Other ◽  
Font Size ◽  
Long Run ◽  
The World ◽  
The Usa ◽  
And Function

<strong><strong></strong></strong><p align="LEFT">I<span style="font-family: DejaVuSerifCondensed; font-size: small;">n the Introduction to this article, I deal with the importance of speaking one’s </span>own language as a way to assert one’s identity. Then I pass on to the evolution of the English language from its start as Old English, spoken by only a few thousand Angles and Saxons.</p><p align="LEFT"><span style="font-family: DejaVuSerifCondensed; font-size: small;">I remark how, at fi rst, it was contaminated by thousands of </span>Latin, French and Scandinavian words, of which contemporary English still bears many clear traces, but nobody has ever thought that English was ever in danger of disappearing. By contrast, in the long run, it became the mother tongue of the speakers in comparatively newly founded countries, such as the USA, Australia, and New Zealand, and owing to the spread of the British Empire, it has dramatically increased its appeal becoming the most spoken and infl uential language in the world. Thus, according to some linguists, it has led several languages virtually to the verge of disappearance. Therefore, I argue whether English has really vampirised them, or has simply contributed to make people understand each other, sometimes even in the same country where lots of diff erent tongues are spoken (e.g. Nigeria).</p><p align="LEFT">It is self-evident that English has gradually been taking the role of a common unifying factor in our globalised world. In this view, I envisage a scenario where English may even become the offi cial l anguage o f the E U with the c ontributions &amp; coming, though in varying doses, from all the speakers of the other EU languages.</p>

scholarly journals Regulation of Gene Expression by Glucose inSaccharomyces cerevisiae: a Role for ADA2and ADA3/NGG1

1999 ◽  
Vol 181 (16) ◽  
pp. 4755-4760 ◽  
Author(s):  
Mei Wu ◽  
Laura Newcomb ◽  
Warren Heideman
Keyword(s):  
Regulation Of Gene Expression ◽  
Deletion Mutants ◽  
Phase Growth ◽  
Transcriptional Responses ◽  
Positive Regulation ◽  
Environmental Signals ◽  
Rapid Induction ◽  
Number Of Genes ◽  
Global Increase

ABSTRACT When Saccharomyces cerevisiae cells are transferred from poor medium to fresh medium containing glucose, they rapidly increase the transcription of a large group of genes as they resume rapid growth and accelerate progress through the cell cycle. Among those genes induced by glucose is CLN3, encoding a G1 cyclin that is thought to play a pivotal role in progression through Start. Deletion of CLN3 delays the increase in proliferation normally observed in response to glucose medium. ADA2 and ADA3/NGG1 are necessary for the rapid induction of CLN3 message levels in response to glucose. Loss of either ADA2 or ADA3/NGG1 also affects a large number of genes and inhibits the rapid global increase in transcription that occurs in response to glucose. Surprisingly, these effects are transitory, and expression of CLN3 and total poly(A)+ RNA appear normal when ADA2 orADA3/NGG1 deletion mutants are examined in log-phase growth. These results indicate a role for ADA2 andADA3/NGG1 in allowing rapid transcriptional responses to environmental signals. Consistent with the role of the Ada proteins in positive regulation of CLN3, deletion of RPD3, encoding a histone deacetylase, prevented the down regulation ofCLN3 mRNA in the absence of glucose.

scholarly journals The role of the integral membrane nucleoporins Ndc1p and Pom152p in nuclear pore complex assembly and function

2006 ◽  
Vol 173 (3) ◽  
pp. 361-371 ◽  
Author(s):  
Alexis S. Madrid ◽  
Joel Mancuso ◽  
W. Zacheus Cande ◽  
Karsten Weis
Keyword(s):  
Electron Microscopy ◽  
Saccharomyces Cerevisiae ◽  
Nuclear Envelope ◽  
Lipid Bilayers ◽  
Nuclear Pore Complex ◽  
Transmembrane Proteins ◽  
Nuclear Pore ◽  
Large Channel ◽  
And Function

The nuclear pore complex (NPC) is a large channel that spans the two lipid bilayers of the nuclear envelope and mediates transport events between the cytoplasm and the nucleus. Only a few NPC components are transmembrane proteins, and the role of these proteins in NPC function and assembly remains poorly understood. We investigate the function of the three integral membrane nucleoporins, which are Ndc1p, Pom152p, and Pom34p, in NPC assembly and transport in Saccharomyces cerevisiae. We find that Ndc1p is important for the correct localization of nuclear transport cargoes and of components of the NPC. However, the role of Ndc1p in NPC assembly is partially redundant with Pom152p, as cells lacking both of these proteins show enhanced NPC disruption. Electron microscopy studies reveal that the absence of Ndc1p and Pom152p results in aberrant pores that have enlarged diameters and lack proteinaceous material, leading to an increased diffusion between the cytoplasm and the nucleus.

scholarly journals Genetic Analysis of the Shared Role of CLN3 and BCK2 at the G1-S Transition in Saccharomyces cerevisiae

Genetics ◽  
1999 ◽  
Vol 153 (3) ◽  
pp. 1131-1143
Author(s):  
Herman Wijnen ◽  
Bruce Futcher
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Analysis ◽  
Target Genes ◽  
S Phase ◽  
Negative Regulator ◽  
Growth Defect ◽  
Uncharacterized Protein ◽  
Number Of Genes ◽  
Transcription Complexes

Abstract The transcription complexes SBF and MBF mediate the G1-S transition in the cell cycle of Saccharomyces cerevisiae. In late G1, SBF and MBF induce a burst of transcription in a number of genes, including G1- and S-phase cyclins. Activation of SBF and MBF depends on the G1 cyclin Cln3 and a largely uncharacterized protein called Bck2. We show here that the induction of SBF/MBF target genes by Bck2 depends partly, but not wholly, on SBF and MBF. Unlike Cln3, Bck2 is capable of inducing its transcriptional targets in the absence of functional Cdc28. Our results revealed promoter-specific mechanisms of regulation by Cln3, Bck2, SBF, and MBF. We isolated high-copy suppressors of the cln3 bck2 growth defect; all of these had the ability to increase CLN2 expression. One of these suppressors was the negative regulator of meiosis RME1. Rme1 induces CLN2, and we show that it has a haploid-specific role in regulating cell size and pheromone sensitivity. Genetic analysis of the cln3 bck2 defect showed that CLN1, CLN2, and other SBF/MBF target genes have an essential role in addition to the degradation of Sic1.

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