scholarly journals Differential expression of individual members of the histone multigene family due to sequences in the 5' and 3' regions of the genes.

1988 ◽  
Vol 8 (5) ◽  
pp. 1887-1895 ◽  
Author(s):  
B J Levine ◽  
T J Liu ◽  
W F Marzluff ◽  
A I Skoultchi
Keyword(s):  
Multigene Family ◽  
Histone Gene ◽  
Mrna Accumulation ◽  
Mrna Pool ◽  
Different Types ◽  
Mouse Cells ◽  
Low Levels ◽  
High Level ◽  
Total Type ◽  
Specific Mrna

Histone proteins are encoded by a multigene family. The H3.2(614) and H2a(614) genes are present as single copies which are expressed at high levels, accounting for 30 to 40% of the H3 and H2a mRNAs, respectively, in different types of mouse cells. The other genes which have been isolated each contribute only a very small amount to the total type-specific mRNA pool. We demonstrate here that the differences in the level of expression of these genes are partly due to differences in their transcription rates. To investigate the sequences responsible for these differences in expression among the members of each family, we carried out DNA-mediated gene transfer experiments with both intact and chimeric histone genes. The 5' region of a highly expressed gene [H3.2(614) or H2a(614)] was attached to the 3' region of a histone gene which was expressed at low levels (H3-221 or H2a-291) and vice versa. The results show that sequences in both the 5' and 3' regions of the H3.2(614) and H2a(614) genes contribute to their high level of mRNA production by two independent mechanisms. The effect of the 3' sequences on mRNA accumulation has been narrowed to a 65-base-pair region including the 3'-terminal palindrome and downstream signal implicated in mRNA processing.

Differential expression of individual members of the histone multigene family due to sequences in the 5' and 3' regions of the genes

1988 ◽  
Vol 8 (5) ◽  
pp. 1887-1895
Author(s):  
B J Levine ◽  
T J Liu ◽  
W F Marzluff ◽  
A I Skoultchi
Keyword(s):  
Multigene Family ◽  
Histone Gene ◽  
Mrna Accumulation ◽  
Mrna Pool ◽  
Different Types ◽  
Mouse Cells ◽  
Low Levels ◽  
High Level ◽  
Total Type ◽  
Specific Mrna

Histone proteins are encoded by a multigene family. The H3.2(614) and H2a(614) genes are present as single copies which are expressed at high levels, accounting for 30 to 40% of the H3 and H2a mRNAs, respectively, in different types of mouse cells. The other genes which have been isolated each contribute only a very small amount to the total type-specific mRNA pool. We demonstrate here that the differences in the level of expression of these genes are partly due to differences in their transcription rates. To investigate the sequences responsible for these differences in expression among the members of each family, we carried out DNA-mediated gene transfer experiments with both intact and chimeric histone genes. The 5' region of a highly expressed gene [H3.2(614) or H2a(614)] was attached to the 3' region of a histone gene which was expressed at low levels (H3-221 or H2a-291) and vice versa. The results show that sequences in both the 5' and 3' regions of the H3.2(614) and H2a(614) genes contribute to their high level of mRNA production by two independent mechanisms. The effect of the 3' sequences on mRNA accumulation has been narrowed to a 65-base-pair region including the 3'-terminal palindrome and downstream signal implicated in mRNA processing.

PSYCHOSEMANTIC FEATURES OF PERCEPTION OF ANIMATED ADVERTISEMENT OF YOUTH TARGET AUDIENCE

2020 ◽  
pp. 188-208
Author(s):  
O. P. Sosniuk ◽  
І. Yu. Vlasiuk
Keyword(s):  
Emotional Intelligence ◽  
Empirical Research ◽  
Age Groups ◽  
Target Audience ◽  
Consumer Motivation ◽  
Different Types ◽  
Low Levels ◽  
High Level ◽  
The Relationship

The article is devoted to the analysis of the results of empirical research of psychosemantic features of perception of animation advertising by the representatives of the youth target audience. Differences in the perception of animation advertising between representatives of three age groups within the youth target audience were established. The authors identified four factors (“Confidence”, “Attractiveness”, “Trust”, “Consumer Activity”), which are manifested in the perception of animation advertising by young teens (12-15 y.o.). At the same time, for аdolescence teens (16-19 y.o.) and youth (20-24 y.o.) there are three factors (“Confidence”, “Attractiveness”, “Consumer activity”), which determine their attitude to animation advertising. The peculiarities of young people's attitude to animation advertising with different types of graphics (2-D, 3-D, combined) and consumer motivation of this target audience are revealed. It has been identified that commercials based on the use of 3-D graphics are evaluated by representatives of the youth audience (of all age groups) most positively and often make them want to buy the products of the advertised brand. Commercials based on the use of 2-D graphics technology attract the most attention of younger teens (12-15 y.o.) and create the appropriate consumer motivation. The commercials created on the basis of the combined graphics are the least interesting to the representatives of the youth target audience. However, the ideas underlying such advertising are the most understandable for youth, and the advertising itself is perceived as relevant and evokes a sense of trust in the product and brand. The connection between the level of emotional intelligence and creativity with the peculiarities of the perception of animation advertising is revealed. Subjects with higher level of emotional intelligence rate animation graphics in advertising more positively than people with low levels of emotional intelligence. Representatives of the youth target audience (all age groups) with high levels of emotional intelligence are most sensitive to animation advertising using 3-D graphics. A similar trend is observed in subjects with a high level of creativity, rate commercials using 3-D graphics higher and perceive it more positively (compared with subjects with lower level of creativity). It is established that young teenagers (12-15 y.o.) with a high level of creativity also positively perceive animation advertising using 2-D graphics technology. Prospects for further research include a more detailed study of the perception of animation advertising with different types of graphics by different age groups, determining the relationship between the perception of animation advertising and the value-semantic sphere of the personality of consumers.

The Implications of Providing Grandchild Care for Grandparents’ Marital Quality

2020 ◽  
Vol 41 (12) ◽  
pp. 2476-2501
Author(s):  
Shuangshuang Wang ◽  
Jan E. Mutchler
Keyword(s):  
Marital Quality ◽  
Health And Retirement Study ◽  
Negative Effects ◽  
Grandparent Caregivers ◽  
Grandchild Care ◽  
Different Types ◽  
Low Levels ◽  
High Level ◽  
Marital Strain ◽  
Retirement Study

This study distinguished among types of grandchild care (i.e., co-residence, high and low levels of babysitting, and no care), and examined their associations with grandparents’ marital quality. The sample consisted of 7,267 married grandparents aged 40 years and over from the 2008, 2010, and 2012 waves of the Health and Retirement Study. Providing grandchild care generally undermined grandparents’ marital quality; however, different types of grandchild care affected different aspects of marital quality. The negative effects of providing grandchild care were more pronounced among grandmothers than grandfathers. Grandmothers providing high-level babysitting care were at especially higher risk of experiencing marital strain among the caregiver groups. Findings suggest that providing grandchild care appears to be more of a stressor than a source of reward with respect to shaping grandparents’ marital quality. Sensitivity to such impact on marital quality may be usefully incorporated into developing supports and services meant for grandparent caregivers.

Quota Women Are Threatening to Men

2017 ◽  
Vol 76 (3) ◽  
pp. 107-116 ◽  
Author(s):  
Klea Faniko ◽  
Till Burckhardt ◽  
Oriane Sarrasin ◽  
Fabio Lorenzi-Cioldi ◽  
Siri Øyslebø Sørensen ◽  
...  
Keyword(s):  
Decision Making ◽  
Organizational Performance ◽  
Gender Stereotypes ◽  
Perceived Threat ◽  
Preferential Treatment ◽  
Public Sector Employees ◽  
Different Types ◽  
Low Levels ◽  
Women In Decision Making ◽  
The Impact

Abstract. Two studies carried out among Albanian public-sector employees examined the impact of different types of affirmative action policies (AAPs) on (counter)stereotypical perceptions of women in decision-making positions. Study 1 (N = 178) revealed that participants – especially women – perceived women in decision-making positions as more masculine (i.e., agentic) than feminine (i.e., communal). Study 2 (N = 239) showed that different types of AA had different effects on the attribution of gender stereotypes to AAP beneficiaries: Women benefiting from a quota policy were perceived as being more communal than agentic, while those benefiting from weak preferential treatment were perceived as being more agentic than communal. Furthermore, we examined how the belief that AAPs threaten men’s access to decision-making positions influenced the attribution of these traits to AAP beneficiaries. The results showed that men who reported high levels of perceived threat, as compared to men who reported low levels of perceived threat, attributed more communal than agentic traits to the beneficiaries of quotas. These findings suggest that AAPs may have created a backlash against its beneficiaries by emphasizing gender-stereotypical or counterstereotypical traits. Thus, the framing of AAPs, for instance, as a matter of enhancing organizational performance, in the process of policy making and implementation, may be a crucial tool to countering potential backlash.

High world sugar prices: is Asia’s production cycle to blame?

2010 ◽  
pp. 169-173
Author(s):  
Martin Todd
Keyword(s):  
Supply Chain ◽  
Supply And Demand ◽  
Production Cycle ◽  
Low Levels ◽  
High Level ◽  
High World

The current high world sugar prices reflect a major imbalance between global supply and demand, which has reduced stocks to very low levels. Although it remains to be seen whether prices will rise much above current values, it is clear that the supply chain will remain stretched throughout 2010 and this will help to maintain prices at a high level.

Using Histological and Chemical Methods for Detection of Unauthorized Tissues Addition in Emulsion Type Meat Product

2020 ◽  
Keyword(s):  
Vascular Tissue ◽  
Meat Product ◽  
Calcium Content ◽  
Processed Meat ◽  
Low Protein ◽  
Significant Difference ◽  
Different Types ◽  
Plant Stem ◽  
High Level ◽  
Reliable Methods

Total twenty different processed meat plant producing emulsion type sausage were histologically and chemically examined for detection of adulteration with unauthorized tissues. Results revealed that samples were adulterated with different types of animal tissues included; hyaline cartilage, tendon, spongy bone, peripheral nerve trunk, basophilic matrix, lymphatic tissue, fascia, fibrocartilage and vascular tissue. Moreover, these samples were adulterated Also, adulterated with plant tissue included; plant stem, leaves and root. Chemical analysis showed a significant difference in their chemical composition (moisture, fat, protein, ash and calcium) content. Moisture and fat content varied around the permissible limit of E.S.S. while low protein, high ash and calcium content was detected in the examined samples. Therefore, Histological and chemical examinations can be used as reliable methods to detect adultration using unauthorized addition of both animal and plant tissues in processed meat product samples which revealed a high level of falsification.

Profiles of Citizenship Orientations Among Youth

Young ◽  
2021 ◽  
pp. 110330882110086
Author(s):  
Iana Tzankova ◽  
Gabriele Prati ◽  
Elvira Cicognani
Keyword(s):  
Cluster Analysis ◽  
Young Adults ◽  
Adolescents And Young Adults ◽  
Political Activity ◽  
Late Adolescents ◽  
Institutional Politics ◽  
Different Types ◽  
Political Activist ◽  
Low Levels ◽  
Citizenship Orientations

Prior studies revealed that low levels of youth political activity are not necessarily indicative of complete disengagement from societal affairs but could be accompanied by interest and latent involvement stemming from a standby or monitorial attitude. However, no prior study has investigated patterns of citizenship orientations including both manifest and latent engagement defined by one’s position towards institutional politics, according to different forms of participation. A questionnaire was filled out by 1,732 late adolescents and young adults in Italy (15–30 years old, M = 19.73, 60.7% female). Cluster analysis identified six profiles of citizenship orientations across different types of participatory activities (political, activist, political online and civic): active trustful, active distrustful, standby trustful, standby distrustful, unengaged trustful and unengaged distrustful. The results showed that each level of engagement—active, standby and unengaged—could be further differentiated between trustful and distrustful based on their attitude towards institutions and the electoral process.

scholarly journals Testing Todd: family types and development

2021 ◽  
pp. 1-18
Author(s):  
Jerg Gutmann ◽  
Stefan Voigt
Keyword(s):  
Rule Of Law ◽  
Civil Society Organizations ◽  
The Rule Of Law ◽  
State Fragility ◽  
Family Types ◽  
Low Levels ◽  
Parsimonious Model ◽  
The One ◽  
High Level

Abstract Many years ago, Emmanuel Todd came up with a classification of family types and argued that the historically prevalent family types in a society have important consequences for its economic, political, and social development. Here, we evaluate Todd's most important predictions empirically. Relying on a parsimonious model with exogenous covariates, we find mixed results. On the one hand, authoritarian family types are, in stark contrast to Todd's predictions, associated with increased levels of the rule of law and innovation. On the other hand, and in line with Todd's expectations, communitarian family types are linked to racism, low levels of the rule of law, and late industrialization. Countries in which endogamy is frequently practiced also display an expectedly high level of state fragility and weak civil society organizations.

scholarly journals Space Precession Target Classification Based on Radar High-Resolution Range Profiles

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Yizhe Wang ◽  
Cunqian Feng ◽  
Yongshun Zhang ◽  
Sisan He
Keyword(s):  
Parameter Extraction ◽  
Classification Performance ◽  
Support Vector ◽  
Electromagnetic Data ◽  
Feature Extractor ◽  
Different Types ◽  
Radar Echo ◽  
High Level ◽  
Cone Target

Precession is a common micromotion form of space targets, introducing additional micro-Doppler (m-D) modulation into the radar echo. Effective classification of space targets is of great significance for further micromotion parameter extraction and identification. Feature extraction is a key step during the classification process, largely influencing the final classification performance. This paper presents two methods for classifying different types of space precession targets from the HRRPs. We first establish the precession model of space targets and analyze the scattering characteristics and then compute electromagnetic data of the cone target, cone-cylinder target, and cone-cylinder-flare target. Experimental results demonstrate that the support vector machine (SVM) using histograms of oriented gradient (HOG) features achieves a good result, whereas the deep convolutional neural network (DCNN) obtains a higher classification accuracy. DCNN combines the feature extractor and the classifier itself to automatically mine the high-level signatures of HRRPs through a training process. Besides, the efficiency of the two classification processes are compared using the same dataset.

Flavonoids and UV Photoprotection in Arabidopsis Mutants

2001 ◽  
Vol 56 (9-10) ◽  
pp. 745-754 ◽  
Author(s):  
Ken G Ryan ◽  
Ewald E Swinny ◽  
Chris Winefield ◽  
Kenneth R Markham
Keyword(s):  
Crucial Role ◽  
Hydroxycinnamic Acid ◽  
Ultraviolet B ◽  
Uvb Radiation ◽  
Wild Type ◽  
Hydroxycinnamic Acid Derivatives ◽  
Quercetin Glycosides ◽  
Kaempferol Glycosides ◽  
Low Levels ◽  
High Level

AbstractWild-type Arabidopsis L. leaves exposed to low ultraviolet-B (U V B ) conditions contained predominantly kaempferol glycosides, with low levels of quercetin glycosides. The flavonoid level doubled on treatment with UVB and an increase in the ratio of quercetin: kaempferol was observed. These results suggest that flavonols protect Arabidopsis plants from UVB damage, and indicate that the flavonoid 3’-hydroxylase (F3’H) enzyme, which converts dihydrokaempferol to dihydroquercetin, may play a crucial role. The tt7 mutant lacks this gene and, after treatment with sub-ambient UVB, contained kaempferol glycosides exclusively, to a level of total flavonols similar to that in wild-type Arabidopsis. Total flavonols after enhanced UVB treatment were higher in tt7 than in similarly treated wild-type plants, and only kaempferol glycosides were detected. Despite this high level, tt7 plants were less tolerant of UVB radiation than wild-type plants. These observations suggests that kaempferol is a less effective photoprotectant than quercetin. The chalcone isomerase (CHI) mutant (tt5) surprisingly did not accumulate naringenin chalcone, and this suggests that the mutation may not be restricted to the CHI gene alone. The concentration of hydroxycinnamic acid derivatives did not change with UVB treatment in most varieties indicating that their role in UV photoprotection may be subordinate to that of the flavonoids.

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