scholarly journals Melanin Produced by Bordetella parapertussis Confers a Survival Advantage to the Bacterium during Host Infection

mSphere ◽  
2021 ◽  
Author(s):  
Yukihiro Hiramatsu ◽  
Takashi Nishida ◽  
Dendi Krisna Nugraha ◽  
Fuminori Sugihara ◽  
Yasuhiko Horiguchi
Keyword(s):  
Virulence Factors ◽  
Respiratory Infection ◽  
Bordetella Pertussis ◽  
Survival Advantage ◽  
Etiological Agent ◽  
Negative Bacterium ◽  
Gram Negative ◽  
Content Type ◽  
Bordetella Parapertussis ◽  
Host Infection

In addition to the Gram-negative bacterium Bordetella pertussis , the etiological agent of pertussis, Bordetella parapertussis also causes respiratory infection in humans, with a mild pertussis-like disease. These bacteria are genetically closely related and share many virulence factors, including adhesins and toxins.

scholarly journals Draft Genome Sequence of the Industrially Significant Bacterium Pseudomonas fluorescens ATCC 13525

2018 ◽  
Vol 7 (17) ◽  
Author(s):  
M. J. Meier ◽  
R. M. Subasinghe ◽  
L. A. Beaudette
Keyword(s):  
Antibiotic Resistance ◽  
Pseudomonas Fluorescens ◽  
Virulence Factors ◽  
Draft Genome ◽  
Antibiotic Resistance Genes ◽  
Strain Atcc ◽  
Negative Bacterium ◽  
Gram Negative ◽  
Content Type ◽  
Metabolic Functions

Pseudomonas fluorescens is a Gram-negative bacterium with versatile metabolic functions and potential industrial uses. We sequenced P. fluorescens strain ATCC 13525 with the goal of determining virulence factors and antibiotic resistance genes to predict the potential impacts on human and environmental health in the event of exposure.

scholarly journals Draft Genome Sequence of Pantoea sp. Strain A4, a Rafflesia-Associated Bacterium That ProducesN-Acylhomoserine Lactones as Quorum-Sensing Molecules

2012 ◽  
Vol 194 (23) ◽  
pp. 6610-6610 ◽  
Author(s):  
Kar-Wai Hong ◽  
Han Ming Gan ◽  
Siew-Moon Low ◽  
Patrick Kok Yuen Lee ◽  
Yee-Meng Chong ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Negative Bacterium ◽  
Gram Negative ◽  
Content Type ◽  
First Time

ABSTRACTPantoeasp. strain A4 is a Gram-negative bacterium isolated from theRafflesiaflower. We present here, for the first time, the genome sequence ofRafflesia-associatedPantoeasp. strain A4, which exhibited quorum-sensing activity.

scholarly journals Complete Genome Sequence of Pseudomonas sp. Strain phDV1, an Isolate Capable of Efficient Degradation of Aromatic Hydrocarbons

2019 ◽  
Vol 8 (2) ◽  
Author(s):  
Hao Xie ◽  
Giannis Valsamidis ◽  
Eirini Mathioudaki ◽  
Georgios Tsiotis
Keyword(s):  
Genome Sequence ◽  
Aromatic Hydrocarbons ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Pseudomonas Sp ◽  
Negative Bacterium ◽  
Gram Negative ◽  
Content Type

Pseudomonas sp. strain phDV1 is a Gram-negative bacterium capable of degrading aromatic hydrocarbons. Here, we present the complete genome sequence of this strain, which consists of 4,727,682 bp, with a 62.3% G+C content and 4,574 genes.

scholarly journals Complete Genome Sequence of Klebsiella pneumoniae Strain TK421, a Conjugative Hypervirulent Isolate

2020 ◽  
Vol 9 (3) ◽  
Author(s):  
Travis J. Kochan ◽  
Egon A. Ozer ◽  
Nathan B. Pincus ◽  
Margaret A. Fitzpatrick ◽  
Alan R. Hauser
Keyword(s):  
Klebsiella Pneumoniae ◽  
Genome Sequence ◽  
Nosocomial Infections ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Multidrug Resistant ◽  
Negative Bacterium ◽  
Gram Negative ◽  
Content Type ◽  
Rapid Dissemination

Klebsiella pneumoniae is a Gram-negative bacterium that is a major cause of nosocomial infections worldwide. Here, we present the complete genome sequence of TK421, a clinical bacteremia isolate containing a hypervirulence plasmid carrying tra-associated conjugation machinery genes. Emergence of conjugative hypervirulence plasmids could portend rapid dissemination of hypervirulence among multidrug-resistant K. pneumoniae strains.

scholarly journals Draft Genome Sequences of Two Pseudomonas aeruginosa Isolates from the Female Urogenital Tract

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Genevieve Johnson ◽  
Carine R. Mores ◽  
Alan J. Wolfe ◽  
Catherine Putonti
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Environmental Conditions ◽  
Draft Genome ◽  
Urogenital Tract ◽  
Negative Bacterium ◽  
Genome Sequences ◽  
Gram Negative ◽  
Content Type

Pseudomonas aeruginosa is a Gram-negative bacterium that has the ability to survive in and readily adapt to a variety of environmental conditions. Here, we report 2 genome sequences of P. aeruginosa strains, UMB1046 and UMB5686, isolated from the female urogenital tract.

scholarly journals Multicenter Clinical Evaluation of the Automated AriesBordetellaAssay

2018 ◽  
Vol 57 (2) ◽  
Author(s):  
Ryan F. Relich ◽  
Amy Leber ◽  
Stephen Young ◽  
Ted Schutzbank ◽  
Ronald Dunn ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Clinical Evaluation ◽  
Bordetella Pertussis ◽  
Turnaround Time ◽  
False Positives ◽  
Molecular Methods ◽  
Nasopharyngeal Swab ◽  
Limits Of Detection ◽  
Content Type ◽  
Bordetella Parapertussis

ABSTRACTMolecular methods offer superior sensitivity and specificity and reduce testing turnaround time from days to hours for detection ofBordetella pertussisandBordetella parapertussis. In this study, we evaluated the performance of the automated PCR-based AriesBordetellaAssay, which detects bothB. pertussisandB. parapertussisdirectly from nasopharyngeal swab specimens. The limits of detection (LoDs) were 1,800 CFU·ml−1forB. pertussisand 213 CFU·ml−1forB. parapertussis. The assay detected 16/18 uniqueB. pertussis/B. parapertussisstrains. Of 71 potentially cross-reacting organisms, 5 generated false positives in 1/6 replicates; none of 6 additionalBordetellaspp. were erroneously detected. Specimens were stable at 20 to 25°C for at least 10 h, at 4 to 8°C for 10 days, and at temperatures not exceeding −70°C for 6 months. Of 1,052 nasopharyngeal specimens from patients with suspected pertussis, 3.0% (n= 32) wereB. pertussispositive and 0.2% (n= 2) wereB. parapertussispositive. Combining these data with AriesBordetellaAssay data from 57 nasopharyngeal samples with previously confirmedB. pertussisorB. parapertussisdata and with data from 50 contrivedB. parapertussissamples, the proportions of positive and negative agreement of the respective Aries assays with the reference assays were 97.1% and 99.0% forB. pertussisand 100% and 99.7% forB. parapertussis. The AriesBordetellaAssay provides accurate detection and distinction ofB. pertussisandB. parapertussisinfections within 2 h. (This study has been registered at ClinicalTrials.gov under registration no. NCT02862262.)

scholarly journals Draft Genome Sequences of 42 Environmental Vibrio vulnificus Strains Isolated from the Northern Gulf of Mexico

2019 ◽  
Vol 8 (28) ◽  
Author(s):  
Megan M. Mullis ◽  
I-Shuo Huang ◽  
Githzette M. Planas-Costas ◽  
Reavelyn Pray ◽  
Gregory W. Buck ◽  
...  
Keyword(s):  
Gulf Of Mexico ◽  
Necrotizing Fasciitis ◽  
Vibrio Vulnificus ◽  
Draft Genome ◽  
Opportunistic Pathogen ◽  
Negative Bacterium ◽  
Genome Sequences ◽  
Gram Negative ◽  
Content Type ◽  
Northern Gulf Of Mexico

Vibrio vulnificus is a Gram-negative bacterium and an opportunistic pathogen that can cause septicemia or necrotizing fasciitis. Here, we report the draft genome sequences of 42 environmental V. vulnificus strains collected from the northern Gulf of Mexico. These data will allow for more robust comparisons between clinical and environmental strains.

scholarly journals Genome Sequence of Pedobacter arcticus sp. nov., a Sea Ice Bacterium Isolated from Tundra Soil

2012 ◽  
Vol 194 (23) ◽  
pp. 6688-6688 ◽  
Author(s):  
Ye Yin ◽  
Guidong Yue ◽  
Qiang Gao ◽  
Zhiyong Wang ◽  
Fang Peng ◽  
...  
Keyword(s):  
Cell Surface ◽  
Sea Ice ◽  
Genome Sequence ◽  
Arctic Region ◽  
The Arctic ◽  
Tundra Soil ◽  
Negative Bacterium ◽  
Gram Negative ◽  
Content Type ◽  
The Arctic Region

ABSTRACTPedobacter arcticussp. nov. was originally isolated from tundra soil collected from Ny-Ålesund, in the Arctic region of Norway. It is a Gram-negative bacterium which shows bleb-shaped appendages on the cell surface. Here, we report the draft annotated genome sequence ofPedobacter arcticussp. nov., which belongs to the genusPedobacter.

scholarly journals Identification and Biochemical Characterization of a Novel Protein Phosphatase 2C-Like Ser/Thr Phosphatase in Escherichia coli

2018 ◽  
Vol 200 (18) ◽  
Author(s):  
Krithika Rajagopalan ◽  
Elizabeth Nagle ◽  
Jonathan Dworkin
Keyword(s):  
Escherichia Coli ◽  
Protein Phosphatase ◽  
Biochemical Characterization ◽  
Regulatory Protein ◽  
Negative Bacterium ◽  
Gram Negative ◽  
Content Type ◽  
E Coli ◽  
Novel Protein

Regulatory protein phosphorylation is a conserved mechanism of signaling in all biological systems. Recent phosphoproteomic analyses of phylogenetically diverse bacteria, including the model Gram-negative bacteriumEscherichia coli, demonstrate that many proteins are phosphorylated on serine or threonine residues. In contrast to phosphorylation on histidine or aspartate residues, phosphorylation of serine and threonine residues is stable and requires the action of a partner Ser/Thr phosphatase to remove the modification. Although a number of Ser/Thr kinases have been reported inE. coli, no partner Ser/Thr phosphatases have been identified. Here, we biochemically characterize a novel Ser/Thr phosphatase that acts to dephosphorylate a Ser/Thr kinase that is encoded in the same operon.

scholarly journals New Type of Outer Membrane Vesicle Produced by the Gram-Negative Bacterium Shewanella vesiculosa M7T: Implications for DNA Content

2013 ◽  
Vol 79 (6) ◽  
pp. 1874-1881 ◽  
Author(s):  
Carla Pérez-Cruz ◽  
Ornella Carrión ◽  
Lidia Delgado ◽  
Gemma Martinez ◽  
Carmen López-Iglesias ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Dna Content ◽  
Membrane Vesicle ◽  
Membrane Vesicles ◽  
Freeze Substitution ◽  
Outer Membrane Vesicles ◽  
Negative Bacterium ◽  
Inner Membrane ◽  
Gram Negative ◽  
Content Type

ABSTRACTOuter membrane vesicles (OMVs) from Gram-negative bacteria are known to be involved in lateral DNA transfer, but the presence of DNA in these vesicles has remained difficult to explain. An ultrastructural study of the Antarctic psychrotolerant bacteriumShewanella vesiculosaM7Thas revealed that this Gram-negative bacterium naturally releases conventional one-bilayer OMVs through a process in which the outer membrane is exfoliated and only the periplasm is entrapped, together with a more complex type of OMV, previously undescribed, which on formation drag along inner membrane and cytoplasmic content and can therefore also entrap DNA. These vesicles, with a double-bilayer structure and containing electron-dense material, were visualized by transmission electron microscopy (TEM) after high-pressure freezing and freeze-substitution (HPF-FS), and their DNA content was fluorometrically quantified as 1.8 ± 0.24 ng DNA/μg OMV protein. The new double-bilayer OMVs were estimated by cryo-TEM to represent 0.1% of total vesicles. The presence of DNA inside the vesicles was confirmed by gold DNA immunolabeling with a specific monoclonal IgM against double-stranded DNA. In addition, a proteomic study of purified membrane vesicles confirmed the presence of plasma membrane and cytoplasmic proteins in OMVs from this strain. Our data demonstrate the existence of a previously unobserved type of double-bilayer OMV in the Gram-negative bacteriumShewanella vesiculosaM7Tthat can incorporate DNA, for which we propose the name outer-inner membrane vesicle (O-IMV).

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