scholarly journals A Cold Chain-Independent Specimen Collection and Transport Medium Improves Diagnostic Sensitivity and Minimizes Biosafety Challenges of COVID-19 Molecular Diagnosis

2021 ◽  
Author(s):  
Vikram Saini ◽  
Priya Kalra ◽  
Manish Sharma ◽  
Chhavi Rai ◽  
Vikas Saini ◽  
...  
Keyword(s):  
Molecular Diagnosis ◽  
Urban Population ◽  
Viral Transmission ◽  
Diagnostic Sensitivity ◽  
Cold Chain ◽  
Transport Medium ◽  
Contagious Nature ◽  
Specimen Collection ◽  
Rural And Urban ◽  
Global Population

Approximately forty-four percent of the global population lives in villages, including 59% in Africa ( https://unhabitat.org/World%20Cities%20Report%202020 ). The fast-evolving nature of SARS-CoV-2 and its extremely contagious nature warrant early and accurate COVID-19 diagnostics across rural and urban population as a key to prevent viral transmission. Unfortunately, lack of adequate infrastructure, including the availability of biosafety-compliant facilities and an end-to-end cold chain availability for COVID-19 molecular diagnosis, limits the accessibility of testing in these countries.

A clinical specimen collection and transport medium for molecular diagnostic and genomic applications

2010 ◽  
Vol 139 (11) ◽  
pp. 1764-1773 ◽  
Author(s):  
L. T. DAUM ◽  
S. A. WORTHY ◽  
K. C. YIM ◽  
M. NOGUERAS ◽  
R. F. SCHUMAN ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Genetic Characterization ◽  
Clinical Specimen ◽  
Molecular Transport ◽  
Cold Chain ◽  
Molecular Diagnostic ◽  
Rt Pcr ◽  
Transport Medium ◽  
Specimen Collection

SUMMARYPathogen detection and genetic characterization has dramatically changed in recent years. Clinical laboratories are transitioning from traditional culture and primer-specific sequencing to more robust and rapid nucleic acid testing such as real-time PCR and meta-genomic characterization, respectively. Specimen collection is the first step in any downstream molecular diagnostic procedure. PrimeStore Molecular Transport Medium (MTM) is an optimized blend of nucleic acid stabilizing reagents that includes a non-specific internal positive control that can be amplified using real-time RT–PCR for tracking the integrity of a specimen from the point of collection to detection. PrimeStore MTM is shown here to effectively kill pathogens, including highly pathogenic H5 influenza virus, inactivate nucleases and to protect and preserve released RNA at ambient temperature for up to 30 days for downstream real-time and traditional RT–PCR detection and genetic characterization. PrimeStore MTM is also compatible with a variety of commercial extraction kits. PrimeStore is suited for routine clinical specimens and has added utility for field collection in remote areas, triage centres, border crossings and during pandemics where cold-chain, transport, and dissemination of potentially infectious pathogens are a concern.

scholarly journals ENVIRONMENTAL PSYCHOLOGY: THE URBAN BUILT ENVIRONMENT IMPACT ON HUMAN MENTAL HEALTH

2016 ◽  
Author(s):  
Wang Chen ◽  
Suzaini M. Zaid ◽  
Nurhanisah Nazarali
Keyword(s):  
Mental Health ◽  
Urban Population ◽  
Environmental Psychology ◽  
Eysenck Personality Questionnaire ◽  
Survey Questionnaire ◽  
Environment Impact ◽  
Anova Analysis ◽  
Rural And Urban ◽  
Global Population ◽  
One Way Anova

Growing urban population has increased environmental demands and also affects human health. As the global population becomes more urbanized, there is a concern that it will negatively affect not just physical health, but also mental health. Mental health can be categorized and studied through many different approaches, such as psychology, psychiatry, clinical and sociology. This paper aims to highlight the comparison of mental health of rural and urban dwellers, in terms of environmental psychology (EP) through the Eysenck Personality Questionnaire (EPQ). A survey questionnaire was distributed to respondents in Petaling Jaya to represent the urban area, and Pontian to represent the rural area. The survey questionnaire was analysed using one-way ANOVA analysis, and suggests a relationship between behaviour and age, but also in relation to ‘living area’ influence that suggests urbanization affects a person’s environmental psychology. The findings suggest that urban dwellers are more prone to certain personality traits that can be detrimental to a person’s mental health.

MO759COMPERATIVE STUDY OF THE CARDIOVASCULAR SYSTEM IN PATIENTS WITH CKD ON HEMODIALYSIS IN URBAN AND RURAL POPULATIONS OF UZBEKISTAN

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Olimkhon Sharapov ◽  
Botir Daminov
Keyword(s):  
Heart Failure ◽  
Rural Areas ◽  
Rural Population ◽  
Urban Population ◽  
Medical Center ◽  
Women Patients ◽  
Urban Group ◽  
Rural And Urban ◽  
Ckd Stage ◽  
Rural Group

Abstract Background and Aims According to recently published WHO data, kidney disease has been the 10th leading cause of death in the world over the past 20 years. The lethality of dialysis patients with cardiovascular pathology is 3 times higher than that of patients without CVD. This is especially pronounced in developing countries. Uzbekistan is a country with a population of 34 million and has an equal urban and rural population. In this regard, it is of interest to comparatively study the structure of CVD in patients with CKD stage 5D of the rural and urban population of Uzbekistan. Method We examined 165 (90 men and 75 women) patients with CKD stage 5 receiving dialysis in urban and rural areas of Uzbekistan. The patients were divided into 2 groups depending on their permanent place of residence and the medical center where they receive hemodialysis. 104 (51 men and 53 women) patients were included in the Urban group and 61 (39 men and 22 women) patients were included in the Rural group. The average age of the urban population was 49.7 ± 1.38 years, the rural population was 45.5 ± 1.83 years. All patients underwent programmed hemodialysis according to the standard scheme for 4 hours 3 times a day (12 hours/week). The average duration of hemodialysis in the Urban group was 37.0 ± 4.77 months (M ± m) and 16.6 ± 2.4 months in the Rural group. The main causes of CKD 5D in both groups were glomerulonephritis (Urban-36.5%, Rural-62%) and type 2 diabetes (Urban-31.7%, Rural-18%). Results CVD comorbidity occurred in 55,8% (n=92) of all 165 examined patients, of which 52 were men and 40 were women. The most common CVDs in all groups were hypertension (51%, n=84), coronary heart disease, presented as angina (28%, n=47), heart failure (14%, n=23) and various types of arrhythmias (5%, n=8). 77% (n=127) of patients had anemia due ESRD. In the group Urban(n=104), 62.5% (n=65) had CVD. The main CVD was Hypertension. It was found in 92% (n=60) of patients with CVD in this group. Less (65%, n=42) were patients with angina. Heart failure was detected in 31% (n=20) of patients. Arrhythmia was diagnosed in only 5% (n=5). A large number of combined CVD have been identified. 69% (n=45) of all patients with CVD had a combined CVDs in different combinations. The most common combination was hypertension + angina (n = 26). It accounted for almost 58% of all combined cases. Only 28% (n=17) of all cases with hypertension had "isolated" hypertension. The main combination with hypertension was hypertension + angina (43%, n=26), 11% (n=11) of patients had hypertension + angina + heart failure, a combination in the form of hypertension + angina + arrhythmia had 3% (n=3) patients. Relatively fewer (n = 27, 44%) CVD were found in the Rural group. The most frequent CVD was also a hypertension. Patients with hypertension made up 89% (n = 24) of all patients with CVD in this group. The second place is occupied by angina, it was found in 18.5% (n=5) cases among patients with CVD. Combined CVS pathologies were less common in the rural group. A total of 8 patients (29.6% of all CVD cases) had several CVDs. Conclusion Сardiovascular diseases in the urban population (62.5%) occur almost one and a half times more often than in the rural population (44%). Combined CVD pathology occupies a leading place in the structure of CVD in patients with CKD 5D, both urban and rural.

scholarly journals A CROSS SECTIONAL STUDY ON KNOWLEDGE OF TUBERCULOISIS AMONG RURAL AND URBAN POPULATION OF UDAIPUR DISTRICT, RAJASTHAN

2020 ◽  
pp. 1-2
Author(s):  
Ganesh Salvi ◽  
Rakhi Luthra
Keyword(s):  
Risk Factors ◽  
Pulmonary Tuberculosis ◽  
Urban Population ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Duration Of Treatment ◽  
Cross Sectional ◽  
Rural And Urban ◽  
Tuberculosis Risk

AIM: To assess the knowledge regarding Tuberculosis cause, spread, types and duration of treatment among rural and urban population of Udaipur, Rajasthan. Methodology: A cross sectional study was conducted during January to March 2020 in OPD’s of PHC and UHC of Udaipur city. Total of 216 participants were included. Results: Our study showed that 30.09% were believed that Tb spread form Air, 42.22% were aware about Pulmonary tuberculosis and 53.24% were know that treatment of Tb lasts for 6 months. Conclusion: There is a need to aware people more about Tuberculosis risk factors, symptoms and treatment.

scholarly journals A comparative study on knowledge and perception of vector borne diseases among rural and urban population in Tamil Nadu

2019 ◽  
Vol 6 (6) ◽  
pp. 2672
Author(s):  
Roselin V. ◽  
Srisanthanakrishnan V.
Keyword(s):  
Urban Population ◽  
Tamil Nadu ◽  
Community Education ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Vector Borne Diseases ◽  
Vector Borne Disease ◽  
Rural And Urban ◽  
Vector Borne ◽  
Knowledge And Practice

Background: Vector-borne diseases (VBD) remain a major public health challenge, in India. Knowledge about VBD, social, demographic and environmental factors strongly influence the vector transmission and results in major outbreaks. Hence this study was conducted to assess knowledge and practice along with environmental conditions prevailing in both rural and urban areas.Methods: Cross sectional study was conducted in rural and urban field practice area of Sri Muthukumaran Medical College and Research Institute, Chennai, during June 2018 to December 2018. A total of 472 participants with 236 participants from each urban and rural area were included. Data was collected using proforma and analysis was done using SPSS 16.Results: Knowledge about VBD like dengue was 63.6% and 76.7% among rural and urban population, respectively. Similarly malaria was known by knows 59.3% and 68.2% of rural and urban participants. Japanese Encephalitis was the least known mosquito borne disease in both the groups. (p=0.0136). Common breeding sites addressed by the rural population were artificial collected water (36.9%) and urban population was dirty water (42.8%).Conclusions: Knowledge and practice of preventing vector borne disease is still lacking among both rural and urban participants. Spreading knowledge about VBD is a part in effective vector borne disease control which can be achieved by community education alone rather than insecticides and sprays.

scholarly journals A study to assess the treatment adherence among patients with type 2 diabetes mellitus in rural and urban population of Bengaluru, South India

2020 ◽  
Vol 7 (8) ◽  
pp. 3105
Author(s):  
Usha Rani S. Padmanabha ◽  
Maheswaran R. ◽  
Shwetha Hiremath ◽  
Puneeth N. ◽  
Renuka Prithviraj
Keyword(s):  
Medication Adherence ◽  
Treatment Adherence ◽  
Urban Population ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Middle Income ◽  
Rural And Urban ◽  
The Mean ◽  
Significant Factors

Background: Globally, around 425 million adults are victims of diabetes with increased prevalence being noted in low and middle income countries. Diabetes was seventh leading cause of death leading to 1.6 million deaths in 2016 (WHO report). Management of diabetes is multifaceted which includes diet, physical activity, screening for complications and medications. Adherence to pharmacotherapy plays an important role in glycemic control. Hence, this study was done to assess the magnitude of treatment adherence and its associated factors among type 2 diabetics.Methods: A community based study was done on patients with type 2 DM in the rural and urban population of Bengaluru. A simple random sampling technique was followed. Medication adherence was assessed using 8 item Morisky's treatment adherence scale.Results: A total of 250 subjects were interviewed, among them 59% were male and 41% were female. The mean age of the subjects was 60.93±10.1 years. The mean treatment adherence scores was 25.9±3.1 years. Among the 250 diabetics, 62% were adherent and 38% were non adherent to anti diabetic medications. Forgetfulness to take medications was one of the reasons for non-adherence with mean scores of 3.12+0.6.Being illiterate, labourers, aged above 60 years, obesity, frequency and multiple dosage was found to be significant factors for non-adherence (p<0.05).Conclusions: Adherence to anti diabetic drugs was found to be 62%. Increased age, illiteracy, occupation, BMI, frequency and multiple doses of drugs were significant factors responsible for medication non adherence. There is a need for reinforcement of health education and motivation for diabetics to improve their medication adherence.

Features of the dynamics of hemorrhagic fever with renal syndrome incidence in the Republic of Bashkortostan

2021 ◽  
pp. 44-50
Author(s):  
Pavel Alexandrovich Mochalkin ◽  
◽  
Alexander Pavlovich Mochalkin ◽  
Evgeny Georgievich Stepanov ◽  
Lira Amirovna Farvazova ◽  
...  
Keyword(s):  
Urban Population ◽  
Hemorrhagic Fever ◽  
Forest Steppe ◽  
Rural Settlements ◽  
Administrative District ◽  
Entire Area ◽  
Rural And Urban ◽  
Republic Of Bashkortostan ◽  
The Republic

It was established that 17008 HFRS cases were reported in 53 (out of 54) administrative districts and 12 cities (out of 14) of the Republic of Bashkortostan in 2009-2018. The most intense epidemiological situation (a long-term incidence rate of 48,5 per 100 thousand of the population) was observed within the boundaries of the forest-steppe landscape zone, where. more than 86 % of all cases of infection are recorded of the Republic of Bashkortostan in 2009-2018. The share of infections among the urban population was 64.0 %, the rural one – 36.0 %. In the long-term aspect, according to the conditions of HFRS acquisition, short-term visits to the forest area prevailed – up to 80 %, in household settings – up to 14 % of all cases of the disease. Seasonal increases in the incidence of HFRS take place in May-June – up to 15 % and in September-December – over 46 % of the annual incidence. The presence of synchronicity in the dynamics of HFRS morbidity in rural and urban populations within the boundaries of the administrative district was detected. It has been substantiated that in order to reduce the level of HFRS morbidity among the rural and urban population, it is necessary to provide for an increase in the volume and frequency of preventive (anti-epidemic) measures in the vicinity of rural settlements, regional centers, urban-type settlements and large cities located near forests, within the boundaries of the entire area of the administrative district.

scholarly journals Etiological Factors& Clinical Courses of Birth Asphyxia in Rural and Urban Population of Kishanganj District.

2017 ◽  
Vol 16 (4) ◽  
pp. 554-556
Author(s):  
Dilip Kumar Mandal ◽  
Prasant Kumar ◽  
Uday Shankar Prasad ◽  
Shyamali Datta ◽  
Indranil Dawn ◽  
...  
Keyword(s):  
Urban Population ◽  
Medical Condition ◽  
Perinatal Asphyxia ◽  
Collaborative Study ◽  
Medical Science ◽  
Neonatal Resuscitation ◽  
Birth Asphyxia ◽  
Related Complication ◽  
Maternal Risk ◽  
Rural And Urban

Problem statement : perinatal asphyxia, neonatal or birth asphyxia is a medical condition from deprivation of oxygen to a newborn infant long enough during the process to cause physical usually to the brain. And it is almost all neonatal deaths occur in our rural and urban area. Where the majority is delivered at homes with negligible antenatal care and poor prenatal services.Methods: In this collaborative study conducted prospective, descriptive study. As a case of 150 newborn babies and as a control 1190 newborn babies are fulfilled the selection criteria for prenatal and birth asphyxia .Results: Incidence of birth asphyxia in relation to ante partum and intrapartum factors. And shows that mother with complication like eclampsia, APH, PROM, cord accidents, failed progress of labor, obstructive labor & prolong 2nd stage of labor, etc were more likely to deliver asphyxiated baby, and analysis of maternal risk factors for birth asphyxia. Many pathological, biochemical & metabolic changes occurs as a result of birth asphyxia. And the data were analyzed by slandered statistical test, namely, Z test, Chi square test, and uniovariate and ultivariate logistic regression analysis of risk factor.Conclusion: In our study it was observed that, Pregnancy related complication in rural & urban population of Kishanganj district was mostly Eclampsia, pre-eclamptic toxaemia, Oligohydramnios, PROM(M24hr) etc. To prevent birth asphyxia trained personal and neonatal resuscitation equipment should be mandatory in all maternity home/hospital because prevention is the best and be only option to reduce the Pre natal & birth asphyxia.Bangladesh Journal of Medical Science Vol.16(4) 2017 p.554-556

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