Lipid-induced changes in protein conformation as a means to regulate the immunogenicity of antigens incorporated in tubular immunostimulating complexes

BIOPHYSICS ◽  
2016 ◽  
Vol 61 (3) ◽  
pp. 380-386 ◽  
Author(s):  
N. M. Sanina ◽  
N. S. Vorobieva ◽  
O. D. Novikova ◽  
O. Yu. Portniagina ◽  
L. A. Davydova ◽  
...  
Keyword(s):  
Protein Conformation ◽  
Induced Changes ◽  
Immunostimulating Complexes

Real-time assessment of possible electromagnetic-field-induced changes in protein conformation and thermal stability

2014 ◽  
Vol 35 (7) ◽  
pp. 470-478 ◽  
Author(s):  
Christian Beyer ◽  
Philipp Christen ◽  
Ilian Jelesarov ◽  
Jürg Fröhlich
Keyword(s):  
Thermal Stability ◽  
Electromagnetic Field ◽  
Real Time ◽  
Protein Conformation ◽  
Induced Changes ◽  
Time Assessment

Electric field induced changes in protein conformation

Soft Matter ◽  
2014 ◽  
Vol 10 (3) ◽  
pp. 431-437 ◽  
Author(s):  
Innocent Bekard ◽  
Dave E. Dunstan
Keyword(s):  
Electric Field ◽  
Protein Conformation ◽  
Induced Changes

scholarly journals Mapping shifts in nanopore signal to changes in protein and protein-DNA conformation

2020 ◽  
Author(s):  
A. Carlsen ◽  
V. Tabard Cossa
Keyword(s):  
Protein Conformation ◽  
Signal Analysis ◽  
Structural Integrity ◽  
Geometric Model ◽  
Geometric Approach ◽  
Dna Conformation ◽  
Experimental Conditions ◽  
Biomolecular Structure ◽  
Induced Changes ◽  
Important Progress

ABSTRACTSolid-state nanopores have been used extensively in biomolecular studies involving DNA and proteins. However, the interpretation of signals generated by the translocation of proteins or protein-DNA complexes remains challenging. Here, we investigate the behavior of monovalent streptavidin and the complex it forms with short biotinylated DNA over a range of nanopore sizes, salts and voltages. We describe a simple geometric model that is broadly applicable and employ it to explain observed variations in conductance blockage and dwell time with experimental conditions. The general approach developed here underscores the value of nanopore-based protein analysis and represents progress toward the interpretation of complex translocation signals.STATEMENT OF SIGNIFICANCENanopore sensing allows investigation of biomolecular structure in aqueous solution, including electric-field-induced changes in protein conformation. This nanopore-based study probes the tetramer-dimer transition of streptavidin, observing the effects of increasing voltage with varying salt type and concentration. Binding of biotinylated DNA to streptavidin boosts the complex’s structural integrity, while complicating signal analysis. We describe a broadly applicable geometric approach that maps stepwise changes in the nanopore signal to real-time conformational transitions. These results represent important progress toward accurate interpretation of nanopore signals generated by macromolecular complexes.

scholarly journals Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Michelle R. Baker ◽  
Sean K. Benton ◽  
Christopher S. Theisen ◽  
Chad A. McClintick ◽  
Eugene E. Fibuch ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Chemical Modification ◽  
Protein Conformation ◽  
Cross Linking ◽  
Molecular Aspect ◽  
Release Agent ◽  
Persistent Effect ◽  
Irreversible Effect ◽  
Induced Changes

Persistent alteration of protein conformation due to interaction with isoflurane may be a novel molecular aspect of preconditioning. We preincubated human serum albumin with isoflurane, dialyzed to release agent, and assessed protein conformation. Susceptibility to chemical modification by methylglyoxal and nitrophenylacetate was also examined. Isoflurane had a persistent effect on protein conformation. An increase in the susceptibility of surface residues to chemical modification attended this change in conformation. Modification of isoflurane-treated HSA included intra- and intersubunit cross-linking that may be a consequence of anesthetic-induced changes in multimeric subpopulations. This irreversible effect of isoflurane may represent a mechanism for preconditioning.

scholarly journals Time-Resolved Polarized Fluorescence of C-Phycocyanin and Its Subunits from Mastigocladus laminosus

1984 ◽  
Vol 39 (6) ◽  
pp. 606-616 ◽  
Author(s):  
P. Hefferle ◽  
P. Geiselhart ◽  
T. Mindl ◽  
S. Schneider ◽  
W. John ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Protein Conformation ◽  
Streak Camera ◽  
Fluorescence Decay ◽  
Polarized Fluorescence ◽  
Fluorescence Depolarization ◽  
Time Resolved ◽  
Α Subunit ◽  
Induced Changes ◽  
Kinetics Of

The influence of aggregation and temperature on the excited state kinetics of C-phycocyanin from Mastiqocladus laminosus has been studied. Polarized fluorescence decay curves have been recorded using a synchronously pumped dye laser in conjunction with a synchroscan streak camera. The experimental data for all samples can be fit satisfactorily assuming a biexponential decay law. Fluorescence depolarization times have been interpreted in terms of energy transfer among the different chromophores. The influence of temperature is only moderate on the intra­molecular relaxation, but pronounced on the rates of energy transfer. Both are dependent on the size of the aggregate. The biexponential decay of the α-subunit containing only one chromophore, indicates the presence of different subsets of chromophores in these samples. The results are discussed in terms of variations of the chromophore arrangements upon temperature induced changes in the protein conformation.

Interpretation of irradiation-induced changes in electron diffractograms and images of extinction contours at low temperatures

1984 ◽  
Vol 42 ◽  
pp. 268-269
Author(s):  
E. Knapek ◽  
H. Formanek ◽  
G. Lefranc ◽  
I. Dietrich
Keyword(s):  
Low Temperatures ◽  
Carbon Films ◽  
Organic Crystals ◽  
Wide Spread ◽  
Lens System ◽  
Preparation Conditions ◽  
Thin Carbon ◽  
Electron Diffractograms ◽  
Diffraction Patterns ◽  
Induced Changes

A few years ago results on cryoprotection of L-valine were reported, where the values of the critical fluence De i.e, the electron exposure which decreases the intensity of the diffraction reflections by a factor e, amounted to the order of 2000 + 1000 e/nm2. In the meantime a discrepancy arose, since several groups published De values between 100 e/nm2 and 1200 e/nm2 /1 - 4/. This disagreement and particularly the wide spread of the results induced us to investigate more thoroughly the behaviour of organic crystals at very low temperatures during electron irradiation.For this purpose large L-valine crystals with homogenuous thickness were deposited on holey carbon films, thin carbon films or Au-coated holey carbon films. These specimens were cooled down to nearly liquid helium temperature in an electron microscope with a superconducting lens system and irradiated with 200 keU-electrons. The progress of radiation damage under different preparation conditions has been observed with series of electron diffraction patterns and direct images of extinction contours.

Drug Induced Changes in Cell Organelles

1968 ◽  
Vol 26 ◽  
pp. 110-111
Author(s):  
Sarah A. Luse
Keyword(s):  
Clinical Medicine ◽  
Cell Organelles ◽  
Riboflavin Deficiency ◽  
Drug Induced ◽  
New Era ◽  
Health And Disease ◽  
In The Beginning ◽  
Cellular Pathology ◽  
Induced Changes ◽  
The Impact

In the mid-nineteenth century Virchow revolutionized pathology by introduction of the concept of “cellular pathology”. Today, a century later, this term has increasing significance in health and disease. We now are in the beginning of a new era in pathology, one which might well be termed “organelle pathology” or “subcellular pathology”. The impact of lysosomal diseases on clinical medicine exemplifies this role of pathology of organelles in elucidation of disease today.Another aspect of cell organelles of prime importance is their pathologic alteration by drugs, toxins, hormones and malnutrition. The sensitivity of cell organelles to minute alterations in their environment offers an accurate evaluation of the site of action of drugs in the study of both function and toxicity. Examples of mitochondrial lesions include the effect of DDD on the adrenal cortex, riboflavin deficiency on liver cells, elevated blood ammonia on the neuron and some 8-aminoquinolines on myocardium.

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